Use of KikGR a photoconvertible green-to-red fluorescent protein for cell labeling and lineage analysis in ES cells and mouse embryos
© Nowotschin and Hadjantonakis; licensee BioMed Central Ltd. 2009
Received: 6 August 2009
Accepted: 9 September 2009
Published: 9 September 2009
The use of genetically-encoded fluorescent proteins has revolutionized the fields of cell and developmental biology and in doing so redefined our understanding of the dynamic morphogenetic processes that shape the embryo. With the advent of more accessible and sophisticated imaging technologies as well as an abundance of fluorescent proteins with different spectral characteristics, the dynamic processes taking place in situ in living cells and tissues can now be probed. Photomodulatable fluorescent proteins are one of the emerging classes of genetically-encoded fluorescent proteins.
We have compared PA-GFP, PS-CFP2, Kaede and KikGR four readily available and commonly used photomodulatable fluorescent proteins for use in ES cells and mice. Our results suggest that the green-to-red photoconvertible fluorescent protein, Kikume Green-Red (KikGR), is most suitable for cell labeling and lineage studies in ES cells and mice because it is developmentally neutral, bright and undergoes rapid and complete photoconversion. We have generated transgenic ES cell lines and strains of mice exhibiting robust widespread expression of KikGR. By efficient photoconversion of KikGR we labeled subpopulations of ES cells in culture, and groups of cells within ex utero cultured mouse embryos. Red fluorescent photoconverted cells and their progeny could be followed for extended periods of time.
Transgenic ES cells and mice exhibiting widespread readily detectable expression of KikGR are indistinguishable from their wild type counterparts and are amenable to efficient photoconversion. They represent novel tools for non-invasive selective labeling specific cell populations and live imaging cell dynamics and cell fate. Genetically-encoded photomodulatable proteins such as KikGR represent emergent attractive alternatives to commonly used vital dyes, tissue grafts and genetic methods for investigating dynamic behaviors of individual cells, collective cell dynamics and fate mapping applications.
Cell fate, pattern formation and morphogenesis depend on dynamic cell interactions involving a multitude of cell behaviors and cell populations. One way to gain insight into these events is to label and observe single or groups of cells over time. Several approaches have been established for labeling cells in developing mouse embryos, the mammalian genetically-tractable model of choice, including dye injections, electroporation of nucleic acids or proteins into single or groups of cells [1, 2] as well as grafting of genetically-distinct tissues [3, 4] or using chimeras [5–7]. Unfortunately, most of these techniques are invasive, and only effective when the tissue or cells of interest are easy accessible for manipulation. Therefore, it has been a challenge to tag single or groups of cells that are not superficially located.
The advent of genetically-encoded fluorescent proteins has afforded the ability to label cells ubiquitously or selectively depending on the cis-regulatory elements used in transgene design. Whereas native fluorescent proteins are cytosolic , subcellularly-localized fluorescent proteins can be used for higher resolution image information . For example, fluorescent proteins fused to a human histone H2B, since they are bound to active chromatin, allow the visualization and tracking of individual cells within a group [10–12]. Likewise, fluorescent protein fusions that localize to the plasma membrane provide information on membrane dynamics and cell morphology [11, 13]. Thus in combination with increasingly sophisticated imaging technology, for example laser scanning confocal or multiphoton microscopy, and on-stage cultures, genetically-encoded fluorescent proteins can provide high resolution information on dynamic cell behaviors.
Binary genetic approaches such as genetically inducible fate mapping (GIFM) based on the Cre/loxP system can also be used for cell labeling and fate mapping in mice. GIFM is non-invasive and allows the tagging and tracking of non-superficial cell types . However, cell type specificity relies on availability of cis-regulatory elements to drive Cre recombinase transgene expression within specific populations of cells. To date, binary genetic methods have been used to gain information on large groups of cells rather than smaller groups or even individual cells in spatially-defined regions of interest (ROIs). Therefore, methods that combine lineage labeling through transgenesis with spatially-defined ROIs are likely to provide greater flexibility in cell labeling and lineage tracing.
Photomodulatable fluorescent proteins should help overcome the limitations of these approaches by enabling non-invasive selective labeling of cells in ROIs. Various photomodulatable fluorescent proteins including PA-GFP which acquires fluorescence upon activation, PS-CFP2 which changes color from cyan to green fluorescence upon activation, as well as Kaede, KikGR and EosFP which go from a green to a red fluorescence upon activation, have been used to study cell behaviors in different organisms, including Drosophila, chick, Xenopus and zebrafish [15–19], with preliminary proof-of-principle applications recently reported in mice [20, 21]. For example, an alpha-tubulin PA-GFP fusion has used to investigate the dynamics of mesoderm cell migration during Drosophila development , and KikGR has been used to study the migratory behaviors of neural crest cells in chick embryos .
With so many available photomodulatable proteins, choice of the most suitable for any given application and in any particular organism is not obvious as few direct comparisons have been reported. An extensive comparison of PA-GFP, PS-CFP2, Kaede and KikGR for their applicability in cell migratory behavior and cell lineage analysis in the chick embryo has been reported . Importantly, applications in mice are in their infancy and to date are limited one study in pre-implantation embryos  and the characterization of adult transgenic mice. Transgenic strains expressing Kaede have been described in two independent studies [20, 24]. One of these studies investigated the movement of cells of the immune system and employed flow cytometry to analyze photoconverted cells . Therefore, live cell imaging experiments using photomodulatable reporters have yet to be fully explored in mice.
Recognizing the need for a comparison of commonly used photomodulatable fluorescent proteins for their prospective use in mice, we compared PA-GFP, PS-CFP2, Kaede and KikGR. We found the green-to-red photoconvertible protein KikGR, engineered from the coral Favia favus , to be the best suited for cell labeling and fate mapping due to its specific and efficient photoconversion and outstanding brightness compared to the other photomodulatable fluorescent proteins tested in this study. We therefore generated and characterized transgenic mouse strains constitutively expressing KikGR under the CAG promoter . We also report the generation of transgenic ES cell lines exhibiting widespread expression of KikGR. We observed bright fluorescence in KikGR transgenic ES cells, embryos and adult mice. We noted rapid and efficient photoconversion of KikGR at various developmental stages. To study cell dynamics in mouse embryos, groups of cells in spatially defined ROIs were photoconverted and followed over time.
This study therefore provides the first comparison of photomodulatable fluorescent proteins for use in ES cells and mice, and reveals the potential utility of these genetically-encoded reporters for investigating individual cell behaviors, population dynamics and cell fate in the mouse embryo.
Results and Discussion
Photoconversion of cells and ex utero culture of embryos to visualize cell dynamics in situ
Construction and evaluation of different photomodulatable proteins in cells
Comparison of genetically-encoded photomodulatable fluorescent proteins.
Considerable autoactivation (i.e. photoactivation through daylight).
Dim pre-activation. Inefficient photoconversion. Considerable overlap between pre- and post-activation spectral profiles.
Dim. Efficient photoconversion. Aggregates form in COS cells, toxic at when expressed at high levels in ES cells.
Bright pre- and post-activation. Efficient photoconversion.
We next established transgenic ES cell lines constitutively expressing KikGR. Fluorescent transgenic ES cell colonies were identified by their green fluorescence and picked under an epifluorescence stereo dissecting microscope. Clones were expanded and passaged in 96-well plates, and scored for the maintenance and level of green fluorescent reporter after extended maintenance in culture in the absence of selection. Clones that failed to meet these criteria were discarded from further analysis. The photoconversion and photoefficiency of KikGR was quantified in CAG::KikGR transgenic ES cells.
Photoconversion of KikGR in ES cells
Exposure of a circular ROI defined within in an ES cell colony to 405 nm light (30% laser power, 25.0 mW) demonstrated fast photoconversion of KikGR (Figure 4A-E). Complete photoconversion was reached in 79 seconds under the experimental conditions used. Continued exposure of photoconverted cells eventually lead to photobleaching of the fluorophore and a slight reduction in red fluorescence (Figure 4G-H). Fluorescent intensities of the green and red channel plotted over time illustrated the rapid shift in the emission spectrum from 516 nm to 593 nm after exposure to 405 nm light (Figure 4I).
Expression and function of KikGR in adult transgenic mice and post-implantation embryos
Live imaging population dynamics using KikGR
Coordinated behaviors within cell populations are integral to a wide variety of morphogenetic events. Using transgenesis to direct KikGR expression in embryos and photoconversion of ROIs in combination with 3D time-lapse imaging and ex utero embryo culture, the collective cell behaviors directing the morphogenesis of the mouse embryo can be probed. For example, given the precision of cell labeling using defined ROIs, it can be determined if collective cell behaviors such as convergence and extension occur in mouse embryos. A convergence and extension type cell behavior can be investigated through photoconversion of cells contained in a rectangular ROI, and measurement of the height-to-width over time. One can then determine if the starting ratio is maintained or skews in any particular dimension over time, with the latter trend suggestive of a convergent extension type cell behavior.
Live imaging of cell fate in the mouse embryo
Tracking cells over time using 3D time-lapse imaging and on-stage mouse embryo culture
We have compared four widely available photomodulatable fluorescent proteins: PA-GFP which photoactivates to produce green fluorescence, the cyan-to-green PS-CFP2, and Kaede and KikGR, two green-to-red photoconvertible fluorescent proteins. Our data demonstrate that the photomodulatable fluorescent protein KikGR is suitable for a number of live imaging applications in ES cells and in mouse embryos. KikGR is developmentally-neutral, does not autoactivate after exposure to broad spectrum white light, undergoes rapid and complete photoconversion when exposed to light of a specific wavelength, and exhibits bright fluorescence before and after photoconversion.
CAG::KikGR transgenic mice lend themselves to a variety of live imaging applications for investigating individual cell behaviors, population dynamics and cell fate in the mouse embryo. Defined ROIs permit high temporal and spatial specificity for cell labeling, yielding high-resolution information on cell dynamics in vivo. Having established widespread expressing KikGR strains and demonstrated their utility, the development of strains exhibiting lineage-specific KikGR should in the future should provide greater spatial specificity for cell labeling. Moreover, the future development of mouse strains expressing subcellularly-localized KikGR or monomeric KikGR fusion proteins should provide greater spatial resolution for live imaging.
Plasmids and constructs
CAG::KikGR was generated by PCR of KikGR from pKIkGR1-S1 (MBL), (GenBank Accession number: AB193293)  using oligos EcoRI-KikGR_5': GTTACCGGAATTCCGGATGGTGAGTGTGATTACATCAGAA and EcoRI-KikGR_3': CAATCCGGAATTCCGGTTACTTGGCCAGCCTTGGCAGCCCGGA. The resulting PCR product was cut with EcoRI cloned into pCAGGS . CAG::PA-GFP was generated by PCR of PA-GFP-N1  using oligos EcoRI-PAGFP_5': GTTACCGGAATTCCGGATGGTGAGCAAGGGCGAGGAGCTG and EcoRI-PAGFP_3': CAATCCGGAATTCCGGTTATCTAGATCCGGTGGATCCCGG. CAG::PSCFP2 was generated by PCR of PS-CFP2 from pPS-CFP2-N (Evrogen)  using oligos EcoRI-PSCFP2_5': GTTACCGGAATTCCGGATGAGCAAGGGCGCCGAGCTGTTC and EcoRI-PSCFP2_3': CAATCCGGAATTCCGGTTACTTGTACAGCTCATCCATGCC. The resulting PCR products for each vector were cut with EcoRI and cloned into pCAGGS . CAG::Kaede was generated by PCR of Kaede from pCS2-Kaede  using oligos Kaede-5F-EcoR1: CCGGAATTCCGG ATGGTGAGTCTGATTAAACCAGAAATGAAG and Kaede-3R_EcoR1: CCGGAATTCCGGTTACTTGACGTTGTCCGGCAATCCAGAATG. The resulting PCR products for each vector were cut with EcoRI and cloned into pCAGGS .
Generation of transgenic ES cells
All constructs were tested for fluorescence in COS7 cells and R1 ES cells . R1 ES cells were maintained under standard conditions . Transgenic ES cell lines constitutively expressing CAG::KikGR were generated by co-electroporation of SalI linearized CAG::KikGR construct and a circular PGK-Puro-pA plasmid conferring transient puromycin resistance. Puromycin selection was carried out exactly as described previously .
CAG::KikGR was linearized with SalI and gel-purified using routine protocols  DNA was injected into C57BL/6 zygotes at the Memorial Sloan-Kettering Cancer Center Transgenic Core Facility. F0 founders were screened by PCR and expression of green fluorescence, resulting in 3 founder lines. Founders were mated to ICR females to recover embryos and subsequent F1 generation adults.
Embryo collection and culture
Post-implantation embryos and organs were dissected in DMEM/F12 containing 5% fetal calf serum and cultured in media comprising 50% rat serum, 50% DMEM/F12 supplemented with 1% L-glutamine and 1% Penicillin/Streptomycin.
All images of mouse embryos or organs presented in the figures are of living embryos or freshly dissected (unfixed) tissues maintained under physiological conditions. ES cells were also imaged live. Wide-field images were acquired on a Leica MZFLIII stereo dissecting microscope or Zeiss Axiovert 200 M inverted microscope equipped with epifluorescent illumination using appropriate filter sets. Laser scanning confocal data was acquired using a Zeiss LSM510 META scan head fitted onto a Zeiss Axiovert 200 M. Fluorophores were excited with a 488 nm Argon laser line (green) and a 543 nm HeNe laser (red). Objectives used were a plan-apochromat 20×/0.75 and a plan-apochromat 10×/0.45. Confocal images were acquired as sequential optical x-y sections taken at 1-2 μm z intervals.
COS-7 cell expressing PS-CFP2, KikGR and Kaede were visualized under standard conditions for visualization of CFP and GFP, respectively using a 458 Argon laser (45%) and a 488 nm Argon laser (5% power), respectively, with a plan-apochromat 20×/0.75 objective. A 405 nm Diode laser (set at 35% power) was used for photoconversion of COS7 cells in culture with 1× 50 iterations. Photoconverted KikGR and Kaede were imaged using a 543 nm HeNe laser (80% power) for red fluorescence excitation. Both photoconverted and unconverted KikGR and Kaede were imaged using 488 nm Ag laser (5% power) for green fluorescence and 543 nm HeNe laser (80% power) for red fluorescence in multi-track mode. Photoconverted PS-CFP2 was imaged using a 458/488 nm Argon laser (45%/5% laser power) for cyan and green fluorescence respectively, in multi-track mode. Cells and embryos expressing KikGR were visualized under standard conditions for visualization of GFP, using a 488 nm Argon laser (5% power) with a plan-apochromat 20×/0.75 objective. A 405 nm Diode laser (set at 100% power) was used for photoconversion of ES cells in culture with 1 × 50 iterations, and cells in vivo in mouse embryos with 1 × 75 iterations. Photoconverted KikGR was imaged using a 543 nm HeNe laser (80% power) for red fluorescence excitation. Both photoconverted and unconverted KikGR were imaged using 488 nm Ag laser (5% power) for green fluorescence and 543 nm HeNe laser (80% power) for red fluorescence in multi-track mode. For time-lapse imaging after photoconversion, 488 nm and 543 nm lasers (3% and 50% power, respectively) excitation, were used with plan-apochromat 10×/0.45 or plan-apochromat 20×/0.75 objectives capturing 512 × 512 pixels per frame. For quantifying the photoefficiency of KikGR continuous exposure at 405 nm (30% laser power; 25.0 mW) was used.
Raw data was processed using Zeiss LSM software (Carl Zeiss Microsystems at http://www.zeiss.com/) and Adobe Photoshop CS2 (Adobe Systems, Inc., San Jose). Re-animation of data to generate movies of time-lapses was performed using QuickTime Pro (Apple Computer, Inc at http://www.apple.com/quicktime/).
We thank the MSKCC Mouse Genetics Core facility for production of transgenic mice; Jennifer Lippincott-Schwartz and George Patterson for the PA-GFP plasmids; Paul Kulesa and Danny Stark for advice on photoactivation; Rodrigo Fernandez-Gonzalez for assistance with MatLab; Ann Foley for comments on the manuscript. Work in our laboratory is supported by the National Institutes of Health (RO1-HD052115) and NYSTEM. SN is supported by an American Heart Association postdoctoral fellowship.
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