Use of KikGR a photoconvertible green-to-red fluorescent protein for cell labeling and lineage analysis in ES cells and mouse embryos

Table 1 Comparison of genetically-encoded photomodulatable fluorescent proteins.

Fluorescent reporter	Color pre-activation	Color post-activation	Comments
PA-GFP	Non-fluorescent	green	Considerable autoactivation (i.e. photoactivation through daylight).
PS-CFP2	cyan	green	Dim pre-activation. Inefficient photoconversion. Considerable overlap between pre- and post-activation spectral profiles.
Kaede	green	red	Dim. Efficient photoconversion. Aggregates form in COS cells, toxic at when expressed at high levels in ES cells.
KikGR	green	red	Bright pre- and post-activation. Efficient photoconversion.

ISSN: 1471-213X