Figure 9

Live imaging photoconverted KikGR-positive cells in a mouse embryo: photoconversion, ex utero culture and 3D time-lapse laser scanning confocal imaging do not affect embryonic development. Panels show sequence 3D stills over a time of 640 min of a CAG::KikGR^Tg/+ embryo developing from an early head fold stage to an early somite stage (A-G). The series depicts the developing cardiac crescent (cc), headfolds (hf) and condensing somites (som). Two regions of photoconverted cells are shown over time. Cells of photoconverted region located in the left side of the embryo migrate anteriorly into the cardiac crescent. Bright field images (A-G). Merge of RFP channel and bright field (A'-G'). RFP channel (A"-G"). GFP and RFP channel (A"'). m, midline; n, node.