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Figure 4 | BMC Developmental Biology

Figure 4

From: Use of KikGR a photoconvertible green-to-red fluorescent protein for cell labeling and lineage analysis in ES cells and mouse embryos

Figure 4

Efficiency of KikGR photoconversion over time with constant exposure to short wavelength laser light. Continuous scanning over time with 405 nm laser light excitation (30% laser power; 25.0 mW) of an ES cell colony, constitutively expressing KikGR, shows fast and complete photoconversion. Fluorescence in green and red channels at different times of 405 nm exposure (A-H). Yellow dashed ellipse depicts region of interest (ROI) exposed to 405 nm laser light. Plot of time series in A-H shows shift in emission spectra from 513 nm to 596 nm (I).

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BMC Developmental Biology

ISSN: 1471-213X

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