Figure 7
Photoconversion of cells in the lateral plate mesoderm (lpm) of an E8.0 CAG::KikGRTg/+embryo: live imaging of behaviors of cell cohorts. Panels show 2D (bright field) and 3D (green and red channels) laser scanning confocal images of a posterior view of an E8.0 CAG::KikGRTg/+ embryo (A-H). Bright field image (A). KikGR expression in an E8.0 embryo prior to photoconversion. Yellow dashed rectangle, region of interest (ROI), demarcates population of cells to be excited with laser light of 405 nm wavelength (B). Loss of green fluorescence in boxed ROI after photoconversion (C). Cells in boxed ROI fluoresce red after photoconversion (D). Merge of red and green channels after photoconversion showing labeled lpm cells at start of embryo culture (E). Panels show migration of photoconverted cells in lpm of embryo after 6 hrs. Dimensions of cell population photoconverted have become longer and thinner (F-G). Merge of green and red channel (F). Red channel (G). Overlay of red channel onto bright field image (H). ESom, early somite stage; ps, primitive streak. Scale bar indicates 200 μm.