- Research article
- Open Access
Electrochemical patterns during Drosophila oogenesis: ion-transport mechanisms generate stage-specific gradients of pH and membrane potential in the follicle-cell epithelium
BMC Developmental Biology volume 19, Article number: 12 (2019)
Alterations of bioelectrical properties of cells and tissues are known to function as wide-ranging signals during development, regeneration and wound-healing in several species. The Drosophila follicle-cell epithelium provides an appropriate model system for studying the potential role of electrochemical signals, like intracellular pH (pHi) and membrane potential (Vmem), during development. Therefore, we analysed stage-specific gradients of pHi and Vmem as well as their dependence on specific ion-transport mechanisms.
Using fluorescent indicators, we found distinct alterations of pHi- and Vmem-patterns during stages 8 to 12 of oogenesis. To determine the roles of relevant ion-transport mechanisms in regulating pHi and Vmem and in establishing stage-specific antero-posterior and dorso-ventral gradients, we used inhibitors of Na+/H+-exchangers and Na+-channels (amiloride), V-ATPases (bafilomycin), ATP-sensitive K+-channels (glibenclamide), voltage-dependent L-type Ca2+-channels (verapamil), Cl−-channels (9-anthroic acid) and Na+/K+/2Cl−-cotransporters (furosemide). Either pHi or Vmem or both parameters were affected by each tested inhibitor. While the inhibition of Na+/H+-exchangers (NHE) and amiloride-sensitive Na+-channels or of V-ATPases resulted in relative acidification, inhibiting the other ion-transport mechanisms led to relative alkalisation. The most prominent effects on pHi were obtained by inhibiting Na+/K+/2Cl−-cotransporters or ATP-sensitive K+-channels. Vmem was most efficiently hyperpolarised by inhibiting voltage-dependent L-type Ca2+-channels or ATP-sensitive K+-channels, whereas the impact of the other ion-transport mechanisms was smaller. In case of very prominent effects of inhibitors on pHi and/or Vmem, we also found strong influences on the antero-posterior and dorso-ventral pHi- and/or Vmem-gradients. For example, inhibiting ATP-sensitive K+-channels strongly enhanced both pHi-gradients (increasing alkalisation) and reduced both Vmem-gradients (increasing hyperpolarisation). Similarly, inhibiting Na+/K+/2Cl−-cotransporters strongly enhanced both pHi-gradients and reduced the antero-posterior Vmem-gradient. To minor extents, both pHi-gradients were enhanced and both Vmem-gradients were reduced by inhibiting voltage-dependent L-type Ca2+-channels, whereas only both pHi-gradients were reduced (increasing acidification) by inhibiting V-ATPases or NHE and Na+-channels.
Our data show that in the Drosophila follicle-cell epithelium stage-specific pHi- and Vmem-gradients develop which result from the activity of several ion-transport mechanisms. These gradients are supposed to represent important bioelectrical cues during oogenesis, e.g., by serving as electrochemical prepatterns in modifying cell polarity and cytoskeletal organisation.
The development and maintenance of complex multicellular structures, like tissues and organs, is controlled by an interplay of various regulatory processes. Besides genetical and biochemical mechanisms, bioelectrical phenomena, i.e. localised ion fluxes, gradients of ion concentrations, intracellular pH (pHi) and membrane potential (Vmem), are known to function as wide-ranging signals to guide polarity in development, regeneration and wound-healing [1,2,3,4,5]. It has been shown that gradual modifications of bioelectrical properties mediate cellular processes like migration , proliferation , differentiation [8,9,10], and cell-cycle control [11, 12] in various species. For example, Vmem plays a role in specifying the left-right axis in Xenopus and chick embryos , in the regeneration of either head or tail in planarians , in zebrafish pigment-pattern formation , and in planar cell-polarity pathway regulation in Drosophila epithelial tissues . Defects in ion transport have been associated with several human diseases, like e.g. cancer development or Alzheimer’s disease, or with developmental defects caused by human channelopathies [17,18,19,20,21].
The generation and maintenance of electrochemical gradients within cells or tissues requires a polarised distribution and/or activation of specific ion-transport mechanisms [22, 23]. In addition, gap junctions are a prerequisite in coupling groups of cells electrically [24,25,26]. In contrast to classical methods, like e.g., single-cell electrode measurements, new techniques using specific fluorescent probes allow visualisation and analysis of the spatiotemporal characteristics of pHi and Vmem in a large number of cells and in whole tissues [27, 28].
The follicle-cell epithelium (FCE) of the Drosophila ovary provides an appropriate model system for studying bioelectrical phenomena during epithelial development [29,30,31,32,33]. It has been shown that in Drosophila ovarian follicles stage-specific patterns of extracellular currents [29, 30], Vmem [31, 32, 34], and pHi  exist that depend mainly on the exchange of protons, potassium ions and sodium ions [31, 34,35,36]. For studying and manipulating pHi and Vmem in the FCE, we used the fluorescent pH-indicator 5-CFDA,AM and the potentiometric dye DiBAC4(3) as well as several inhibitors of ion-transport mechanisms.
The Drosophila follicle consists of 16 germ-line cells, 15 nurse cells (NC) and one oocyte (Oo), surrounded by a single-layered somatic FCE . During the course of oogenesis, the FCE differentiates into several morphologically distinct follicle-cell (FC) populations (Fig. 1a) [38,39,40]. In addition to establishing embryonic polarity  and building up the eggshell , the FCE plays a significant role in shaping the elongated egg [42, 43], a process which requires planar cell polarity and a polarised arrangement of the cytoskeleton.
The purpose of the present study is to clarify the roles that various ion-transport mechanisms play in regulating pHi and Vmem and in generating stage-specific electrochemical gradients in the FCE. Such gradients are likely to be involved in regulating, e.g., cell polarity, cell migration, and the organisation of the cytoskeleton during oogenesis of Drosophila.
We analysed the pHi in the FCE during the course of oogenesis. In vitellogenic stages (S8–12), we found distinct alterations of the pHi-patterns (Figs. 1b-d and 2a; for typical follicles, see Additional file 1: Figure S1; for variability between follicles of the same stage, see Additional file 2: Table S1).
In S8, the pHi in the FCE is relatively acidic compared to the pHi in the germ-line cells. An antero-posterior (a-p) gradient is visible, with more alkaline pHi in the anterior FC (aFC) and more acidic pHi in the posterior FC (pFC). In S9 and 10a, the a-p gradient reverses and the centripetal FC (cFC) show more acidic pHi than the pFC. At S10b, this a-p gradient becomes very distinct and is preserved during S11 and 12. In contrast to earlier stages, the pHi in the FCE is now more alkaline compared to the pHi in the germ-line cells.
In S8, the FCE does not yet exhibit a dorso-ventral (d-v) gradient. A significant gradient with relatively acidic dorsal and relatively alkaline ventral FC arises in S9 and increases during S10b-12.
During the course of vitellogenesis (S8–12), we also observed distinct changes in the Vmem-patterns (Fig. 2; for typical follicles, see Additional file 1: Figure S2; for variability between follicles of the same stage, see Additional file 2: Table S2).
Up to S8, all FC show a similar Vmem which is relatively depolarised compared to the germ-line cells. At S9 and 10a, an a-p gradient starts to establish with relatively depolarised FC in the centripetal and posterior regions of the follicle (cFC and pFC) compared to the mainbody FC (mbFC). During S10b-12, the gradient changes so that only the pFC are depolarised compared to mbFC and cFC.
During S8-10a, a significant d-v gradient could not be detected in the FCE. However, a d-v gradient emerges during S10b-12, with relative depolarisation on the ventral side of the follicle (vFC). Dorsal cFC show a striking increase in depolarisation during late S10b and 11. But on average, the dorsal FCE is hyperpolarised compared to the ventral FCE.
Inhibition of ion-transport mechanisms
Six inhibitors were used to determine the roles that specific ion-transport mechanisms play in the regulation of pHi and Vmem as well as in the generation of electrochemical gradients in the FCE during S10b. We found that either pHi or Vmem or both parameters were affected by each tested inhibitor.
Effects of inhibitors on pHi
The inhibitors furosemide (Na+/K+/2Cl−-cotransporters), glibenclamide (ATP-sensitive K+-channels), 9-anthroic acid (Cl−-channels) and verapamil (L-type Ca2+-channels) showed significant alkalising effects in the FCE. The inhibitors amiloride (NHEs and Na+-channels) and bafilomycin (V-ATPases) had no significant effects on the pHi in the FCE under these experimental conditions. The strongest effects were observed with both glibenclamide and furosemide (Fig. 3, Additional file 2: Table S3).
The treatment with the inhibitors furosemide, glibenclamide, 9-anthroic acid and verapamil, respectively, resulted again in alkalisation. These effects were significant in all types of FC and particularly distinct with furosemide and glibenclamide (Figs. 4 and 5a, Additional file 2: Table S5). Amiloride led to slight acidification which was significant in pFC. The increase in fluorescence intensity elicited by bafilomycin was due to granular staining in the FCE (Fig. 5 b). Considering the localisation of V-ATPases in plasma membranes and in vesicle membranes of FC [33, 46], it is obvious that bafilomycin caused alkalisation of vesicles, while the cytoplasm of FC became more acidic. Modifications of the a-p and/or d-v pHi-gradients were observed with all tested inhibitors. The influences were particularly strong with both glibenclamide and furosemide, which enhanced the a-p and d-v gradients (increasing angle; alkalisation), as did verapamil. 9-Anthroic acid resulted in a shallower d-v gradient (decreasing angle; acidification) because of its stronger effect on vFC, whereas the a-p gradient became slightly enhanced (alkalisation). Amiloride, on the other hand, led to both shallower a-p and d-v gradients (acidification), as did bafilomycin (increasing cytoplasmic acidification due to increasing vesicle alkalisation). The inclinations of the gradients were inferred from the mean values of relative fluorescence intensity depicted in Figs. 4b and 5a.
Effects of inhibitors on Vmem
Almost all tested inhibitors (verapamil, amiloride, 9-anthroic acid, furosemide and bafilomycin) had significant hyperpolarising effects on the Vmem in the FCE. The strongest effect was observed with verapamil, the weakest with bafilomycin. Glibenclamide caused a slight hyperpolarisation which was not significant. Depolarisation was not observed (Fig. 6, Additional file 2: Table S4).
All inhibitors caused more or less hyperpolarisation in the FCE (Figs. 7 and 8, Additional file 2: Table S6). The strongest effects on Vmem were observed with verapamil and glibenclamide, the weakest with amiloride and bafilomycin (not significant). Glibenclamide and verapamil lowered the a-p gradient as well as the d-v gradient (decreasing angle; hyperpolarisation), whereas furosemide and 9-anthroic acid had prominent decreasing effects only on the a-p gradient. The inclinations of the gradients were inferred from the mean values of relative fluorescence intensity depicted in Figs. 7b and 8.
Stage-specific pHi- and Vmem-gradients are established in the FCE
We have shown that the FCE exhibits stage-specific a-p and d-v gradients of pHi and Vmem, respectively, which are most distinctive during S10b. The pHi- and Vmem-gradients arise approximately at the same stage, but they are not congruent. The pHi increases (alkalisation) from anterior (cFC) to posterior (pFC) and from dorsal (dFC) to ventral (vFC), and, therefore, the dorsal cFC possess the most acidic pHi.
In a-p direction, the developing Vmem-gradient differs from the pHi-gradient. Since the mbFC are relatively hyperpolarised compared to the cFC and pFC, no continuous a-p Vmem-gradient exists. However, from dFC to vFC, the Vmem becomes continuously depolarised. For both pHi and Vmem, the stages differ during which the a-p and d-v gradients become established. Since they start in S9, the a-p gradients are already existing when the d-v gradients become obvious during S10b.
It is expected that the establishment of electrochemical gradients is depending on the asymmetric distribution and/or activity of ion-transport mechanisms in different types of FC. Recent studies have shown that e.g. gap junctions, V-ATPases, Na+/K+-pumps and Na+-channels are enriched in certain regions of the FCE compared to other regions [32, 33, 36, 44,45,46]. To function as temporal and spatial signals during development, electrochemical gradients and/or local changes of pHi and Vmem have to be translated into cellular responses. For example, this could be achieved by voltage-dependent L-type Ca2+-channels that become asymmetrically activated in the FCE .
Inhibitors of ion-transport mechanisms affect pHi and Vmem in the FCE
To identify mechanism that are involved in regulating pHi and Vmem in the FCE, we used inhibitors of several ion-transport mechanisms that have already been detected and/or localised in the Drosophila ovary [32, 33, 44, 47,48,49,50]. All used inhibitors affected either pHi or Vmem or both parameters in S10b (for summary, see Fig. 9). If an inhibitory effect was detectable, it could be observed throughout the entire columnar FCE - though, in different FC types, often to varying extents.
The most prominent effects on pHi were obtained with both glibenclamide (ATP-sensitive K+-channels) and furosemide (Na+/K+/2Cl−-cotransporters). These inhibitors led to strong relative alkalisation in all FC types by blocking H+-transport indirectly. Inhibition of ATP-sensitive K+-channels by glibenclamide reduces K+-efflux. Since the intracellular K+-concentration and the pHi are known to be interrelated, a higher intracellular K+-concentration would reduce H+-influx (K+/H+-antiport [51, 52]). In addition, it has been shown that K+-uptake into Drosophila follicles is strongly dependent on extracellular pH . Na+/K+/2Cl−-cotransport, which can be blocked by furosemide (or bumetanide, an analogue of furosemide), is typically coupled with Cl−/HCO3−-antiport, so that furosemide has no impact on Cl−-transport in total [53, 54]. Due to this coupling, HCO3−-transport might be affected, causing a change of pHi in the FCE [55, 56]. The alkalisation obtained with 9-anthroic acid, which blocks Cl−-channels, is also likely to be due to an effect on Cl−/HCO3−-antiport.
Amiloride (NHEs and Na+-channels) and bafilomycin (V-ATPases), that are both direct inhibitors of H+-transport, led to slight acidification of the FCE. While NHEs are known for their role in pHi-homeostasis [47, 56,57,58], one of the key functions of V-ATPases, besides acidification of cytoplasmic vesicles, is to energise the plasma membranes of most insect cells [59, 60]. Since the generated H+-gradient drives secondary active transport mechanisms like NHEs [51, 60], a strong effect of bafilomycin on pHi was not expected. Similarly, the relatively small impact of amiloride on pHi is supposed to be due to compensatory effects exerted by other ion transporters.
Vmem in the FCE was clearly influenced by glibenclamide (ATP-sensitive K+-channels) and, most strongly, by verapamil (voltage-dependent L-type Ca2+-channels). Although Ca2+-channels are not likely to contribute much to Vmem directly, blocking of Ca2+-influx can result in diverse cellular reactions, since Ca2+ is a second messenger . A strong effect of amiloride on Vmem was not expected, since Na+/H+-antiport is electroneutral. Moreover, the effect of blocking Na+-channels is relatively weak and can be compensated by other ion-transport mechanisms. Compensatory effects always have to be taken into account, and they are supposed to be the reason for the absence of a strong impact of bafilomycin (V-ATPases) on Vmem as well. Similarly, inhibition with furosemide (Na+/K+/2Cl−-cotransporters) and 9-anthroic (Cl−-channels) had only minor effects on Vmem.
Inhibitors of ion-transport mechanisms affect pHi- and Vmem-gradients in the FCE
Since with some inhibitors different types of FC were affected to different extents, the a-p and/or d-v gradients during S10b became modified in several ways (for summary, see Fig. 10).
The treatment with glibenclamide (ATP-sensitive K+-channels) resulted in both an enhancement of the a-p and d-v pHi-gradients (increasing alkalisation) and a reduction of the a-p and d-v Vmem-gradients (increasing hyperpolarisation). This could be due to the higher concentrations of ATP-sensitive K+-channels found in dorsal cFC (unpublished results). It has been shown that both acidic pH and low K+-concentrations result in reduced gap-junctional communication in Drosophila follicles . Therefore, glibenclamide is supposed to enhance gap-junctional communication via higher intracellular K+-concentrations as well as alkalisation of pHi, leading to increasing hyperpolarisation in the Vmem-gradients. Furosemide (Na+/K+/2Cl−-cotransporters) also led to markedly steeper a-p and d-v pHi-gradients as well as to a shallower a-p Vmem-gradient. Whether these effects depend on an unequal distribution of Na+/K+/2Cl−-cotransporters remains to be analysed. Likewise, the distributions of NHEs (amiloride) as well as Cl−-channels (9-anthroic acid), which both modified electrochemical gradients in the FCE, are not yet known. However, it has been shown that V-ATPases are accumulated in the membranes of pFC and vFC [32, 33]. A stronger effect of bafilomycin on these FC types led to reduction of a-p and d-v pHi-gradients (increasing cytoplasmic acidification due to increasing vesicle alkalisation). The application of verapamil both enhanced the a-p and d-v pHi-gradients and reduced the a-p and d-v Vmem-gradients (increasing hyperpolarisation). This is in accordance with the observed higher concentrations of activated L-type Ca2+-channels in pFC and vFC . It is known that high intracellular Ca2+-concentrations result in reduced gap-junctional communication in Drosophila follicles . Therefore, verapamil is supposed to enhance gap-junctional communication by lowering intracellular Ca2+-concentrations, leading to both increasing alkalisation in pHi-gradients and increasing hyperpolarisation in Vmem-gradients.
In the FCE of Drosophila, gap junctions and ion-transport mechanisms, like NHEs, Na+-channels, V-ATPases, ATP-sensitive K+-channels, voltage-dependent L-type Ca2+-channels, Cl−-channels and Na+/K+/2Cl−-cotransporters, are involved in the generation of stage-specific pHi- and Vmem-gradients. Since bioelectrical phenomena are known to affect, e.g., cytoskeletal dynamics, such gradients are supposed to serve as electrochemical prepatterns that control planar cell polarity and guide gradual changes in cytoskeletal organisation. In the FCE, a particular arrangement of cytoskeletal elements is involved in shaping the follicle [42, 43, 62], and stage-specific correlations with pHi- and Vmem-gradients can be observed (unpublished results). Therefore, manipulating the electrochemical gradients and analysing related changes in microfilament and microtubule patterns will help to understand the influence of bioelectrical cues during development (I. Weiß and J. Bohrmann, manuscript in prep.).
Preparation of Drosophila follicles
Oregon R (wild-type) Drosophila melanogaster were reared at 20–23 °C on standard medium with additional fresh yeast. 2–3 days old females were killed by crushing the head and thorax with tweezers without anaesthesia. The ovaries were dissected and single follicles of all stages (S1–14) were isolated. The preparations were carried out in R-14 medium  which is best suited for in-vitro culture of Drosophila follicles .
Analysis of intracellular pH
Intracellular pH (pHi) of FC was analysed using the pH-sensitive fluorescent dye CFDA (5-carboxyfluorescein diacetate, acetoxymethyl ester; 5-CFDA,AM; Molecular Probes/Thermo Fisher Scientific, USA) [27, 32]. Relative fluorescence intensities were stated, i. e. lower fluorescence intensity indicates lower pHi (more acidic) while higher fluorescence intensity indicates higher pHi (more alkaline). Living follicles were incubated for 20 or 60 min, depending on the type of experiment (see below), in R-14 medium containing 4 μM CFDA (dissolved in dimethyl sulfoxide; DMSO). For controls, see Additional file 1: Fig. S3.
Analysis of membrane potential
To analyse membrane potentials (Vmem) of FC, the voltage-sensitive fluorescent dye DiBAC (bis-(1,3-dibutylbarbituric acid) trimethine oxonol; DiBAC4(3); Molecular Probes) was used [28, 32]. Relative fluorescence intensities were stated, i. e. lower fluorescence intensity indicates relative hyperpolarisation and higher fluorescence intensity indicates relative depolarisation. Living follicles were incubated for 20 or 60 min, depending on the type of experiment (see below), in R-14 medium containing 4 μM DiBAC (dissolved in 70% ethanol).
Fluorescence microscopy and optical sectioning
To investigate pHi and Vmem as well as their changes in detail, two types of experiments were performed. ImageJ (NIH, USA) was used to generate pseudocolour images.
Groups of four to ten follicles were imaged for 60 min during staining in covered glass block dishes on a Zeiss Axiovert 200 wide-field fluorescence microscope (WFM), equipped with a Hamamatsu Orca ER camera, using a × 5 objective and a × 1.6 optovar. An image was taken every 5 min. Settings and exposure time remained unchanged.
Single follicles were imaged in R-14 medium after staining for 20 min on a Zeiss AxioImager.M2 structured-illumination microscope (SIM), equipped with a Zeiss ApoTome and a Zeiss AxioCamMRm camera, using a × 20 objective. Median optical sections were produced as shown in Fig. 2 a.
Inhibition of ion-transport mechanisms
To compensate for the variability observed between different flies, all S10b-follicles of a single fly (approximately 10–20 follicles) were divided into a control group and an experimental group. Inhibition and staining of living follicles, using either CFDA or DiBAC, were done simultaneously for 20 or 60 min, depending on the type of experiment, in R-14 medium containing the respective inhibitor and the fluorescent probe.
The following inhibitors of ion-transport mechanisms were used: Na+/H+-exchangers (NHE) and amiloride-sensitive Na+-channels were blocked with amiloride (Sigma-Aldrich, Germany; 10 μM; dissolved in DMSO), V-ATPases with bafilomycin A1 (Sigma-Aldrich; 160 nM; dissolved in DMSO), ATP-sensitive K+-channels with glibenclamide (Biomol, Germany; 100 μM; dissolved in DMSO), voltage-dependent L-type Ca2+-channels with verapamil-HCl (Sigma-Aldrich; 50 μM; dissolved in ethanol), Cl−-channels with 9-anthroic acid (Sigma-Aldrich; 100 μM; dissolved in ethanol) and Na+/K+/2Cl−-cotransporters with furosemide (Sigma-Aldrich; 1 mM; dissolved in DMSO). Control experiments were performed in R-14 medium containing 0.1–1% v/v ethanol or DMSO, respectively, without the inhibitor.
Quantitative analysis of fluorescence intensities of CFDA and DiBAC
The original grey-scale images were used to measure the fluorescence intensities in the FCE with ImageJ (“mean grey value”).
WFM-images of each point of time were evaluated by measuring the columnar FCE of every single follicle. The mean grey values of the experimental group and the control group, respectively, were averaged (all follicles from the same fly). To compare the long-term effects of the inhibitors on either pHi or Vmem, the averaged values of each point of time were summed up and normalised. Three experiments for each inhibitor were included in this evaluation (mean intensity ratio).
SIM-images were used to measure individual regions of different types of FC: cFC, mbFC, pFC, dFC and vFC (see Fig. 1 a). All mean grey values of each FC type of the experimental group and the control group, respectively, were averaged for comparative analysis (all follicles from the same fly). For each inhibitor, at least five repetitions of the experiment were performed. The normalised values of the single experiments were merged into one evaluation (relative intensity).
Multiple t-tests with Holm-Sidak correction, Microsoft Excel and GraphPad Prism were used for statistical analysis. Graphpad Prism was also used for representation of the data.
Availability of data and materials
The datasets used during the current study are available from the corresponding author on reasonable request.
anterior follicle cells
centripetal follicle cells
- CFDA :
5-carboxyfluorescein diacetate, acetoxymethyl ester
bis-(1,3-dibutylbarbituric acid) trimethine oxonol
mainbody follicle cells
posterior follicle cells
- pHi :
- Vmem :
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We are indepted to Susanne Schotthöfer for technical assistance.
IW carried out the experiments and analysed the data under the supervision of JB. JB conceived the study and reviewed the data. Both authors wrote the manuscript and read and approved the final version.
Financial support by RWTH Aachen University is acknowledged.
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Figure S1. Development of pHi-gradients in the FCE during S8–12 (CFDA; SIM). Examples corresponding to Fig. 1b. Figure S2. Development of Vmem-gradients in the FCE during S8–12 (DiBAC; SIM). Examples corresponding to Fig. 2b. Figure S3. Controls for pHi (CFDA; WFM). Examples of S10b follicles incubated for 60 min in R-14 medium pH 5.5 (hydrochloric acid) and pH 8.0 (sodium hydroxide), respectively. (PPTX 9873 kb)
Table S1. Development of pHi-gradients in the FCE during S8–12 (CFDA; SIM). Numerical values corresponding to Fig. 1 c. Table S2. Development of Vmem-gradients in the FCE during S8–12 (DiBAC; SIM). Numerical values corresponding to Fig. 2 c. Table S3. Inhibitors of ion-transport mechanisms exert influence on the pHi in the FCE during S10b (WFM-experiment; CFDA). Numerical values corresponding to Fig. 3a. Table S4. Inhibitors of ion-transport mechanisms exert influence on the Vmem in the FCE during S10b (WFM-experiment; DiBAC). Numerical values corresponding to Fig. 6a. Table S5. The a-p and d-v pHi-gradients in the FCE are affected by all inhibitors in S10b (SIM-experiment; CFDA). Numerical values corresponding to Figs. 4b and 5a. Table S6. The a-p Vmem-gradient in the FCE is affected by most inhibitors in S10b. Some inhibitors exert influence on the d-v Vmem-gradient (SIM-experiment; DiBAC). Numerical values corresponding to Figs. 7b and 8. (PPTX 78 kb)
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Weiß, I., Bohrmann, J. Electrochemical patterns during Drosophila oogenesis: ion-transport mechanisms generate stage-specific gradients of pH and membrane potential in the follicle-cell epithelium. BMC Dev Biol 19, 12 (2019) doi:10.1186/s12861-019-0192-x
- Drosophila melanogaster
- Follicle cell
- Intracellular pH
- Membrane potential
- Cell polarity
- Pattern formation
- Ion pump
- Ion channel