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Fig. 7 | BMC Developmental Biology

Fig. 7

From: Electrochemical patterns during Drosophila oogenesis: ion-transport mechanisms generate stage-specific gradients of pH and membrane potential in the follicle-cell epithelium

Fig. 7

The a-p Vmem-gradient in the FCE is affected by most inhibitors during S10b (SIM-experiment; DiBAC). a Pseudocolour SIM-fluorescence images of S10b-follicles. Compared to the control (ethanol), blocking with verapamil for 20 min resulted in strong hyperpolarisation of the FCE. b Glibenclamide and verapamil led to strong hyperpolarisation in all FC types, but especially in pFC, thus lowering the inclination of the a-p gradient (angle × 0.5 and × 0.3, respectively). Furosemide and 9-anthroic acid hyperpolarised only the pFC, thereby lowering the a-p gradient as well (both × 0.5). Amiloride and bafilomycin showed no significant effects on the a-p gradient (cf. Additional file 2: Table S6). For statistics, see legend to Fig. 4

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