The Aurora A-HP1γ pathway regulates gene expression and mitosis in cells from the sperm lineage
© Leonard et al.; licensee BioMed Central. 2015
Received: 20 June 2014
Accepted: 12 May 2015
Published: 29 May 2015
HP1γ, a well-known regulator of gene expression, has been recently identified to be a target of Aurora A, a mitotic kinase which is important for both gametogenesis and embryogenesis. The purpose of this study was to define whether the Aurora A-HP1γ pathway supports cell division of gametes and/or early embryos, using western blot, immunofluorescence, immunohistochemistry, electron microscopy, shRNA-based knockdown, site-directed mutagenesis, and Affymetrix-based genome-wide expression profiles.
We find that the form of HP1γ phosphorylated by Aurora A, P-Ser83 HP1γ, is a passenger protein, which localizes to the spermatozoa centriole and axoneme. In addition, disruption in this pathway causes centrosomal abnormalities and aberrations in cell division. Expression profiling of male germ cell lines demonstrates that HP1γ phosphorylation is critical for the regulation of mitosis-associated gene expression networks. In female gametes, we observe that P-Ser83-HP1γ is not present in meiotic centrosomes of M2 oocytes, but after syngamy, it becomes detectable during cleavage divisions, coinciding with early embryonic genome activation.
These results support the idea that phosphorylation of HP1γ by Aurora A plays a role in the regulation of gene expression and mitotic cell division in cells from the sperm lineage and in early embryos. Combined, this data is relevant to better understanding the function of HP1γ in reproductive biology.
KeywordsEpigenetics Heterochromatin Protein 1 HP1γ CBX3 Gametes Preimplantation embryo Meiosis Mitosis Embryonic genome activation
Strong experimental evidence generated during the last decade has established that epigenetic mechanisms are both necessary and required for the development and maintenance of reproductive function . At the mechanistic level, epigenetic regulation primarily consists of establishing, maintaining, and reversing posttranslational modifications (chemical marks) present on DNA and histones, as well as the function of several types of both small and large non-coding RNA molecules . Within a particular cell type, these epigenetic regulators function as signals that define gene expression patterns, which establish and maintain normal and diseased phenotypes. Epigenetic marks are generated by a highly specialized group of proteins responsible for their deposition (writers), interpretation (readers), and when necessary, erasure from chromatin (erasers). The HP1 family of reader proteins was one of the first discovered epigenetic regulators and most versatile of the proteins that regulate chromatin-based inheritance . There are three HP1 subtypes α, β, and γ, encoded in humans by CBX5, CBX1, and CBX3, respectively . These proteins have three domains with the N-terminal chromodomain binding to lysine 9 methylated histone marks, joined by a flexible linker region to a carboxy-terminal chromoshadow domain, which supports dimerization and interaction with other chromatin proteins . HP1α and HP1β are mostly associated with transcriptionally inactive heterochromatin, whereas HP1γ is observed in both heterochromatin and transcriptionally active euchromatin . Functionally, HP1 proteins regulate development through their ability to determine various cellular phenotypes by regulating entire gene expression networks at the appropriate level, time, and place. HP1 proteins are also emerging as candidate regulators of reproductive function; however, their roles in these phenomena have just begun to be elucidated. For instance, recent studies demonstrate that genetic inactivation of HP1γ in the germ line results in adult males carrying severe spermatogenic defects and growth retardation [7, 8]. Interestingly, previous work in somatic cells has shown that HP1γ is phosphorylated, acetylated, ubiquitinated, sumoylated, and methylated , though whether these posttranslational modifications have any impact on the biology of cells from reproductive organs remains to be defined. In the current study, we describe the phosphorylation, localization, and genome-wide regulatory functions of HP1γ in gonadal tissue, gametes, and the pre-implantation embryo. We demonstrate that phosphorylation of this protein at S83, which occurs in response to Aurora A , is necessary for supporting proper mitotic cell division in cells from the sperm lineage. Therefore, we conclude that phosphorylation of HP1γ, prior to meiosis, differentiation, and maturation, is necessary to maintain a viable pool of male gametes, extending our understanding of signaling cascades that regulate the function of this important chromatin protein in reproductive biology. Since drugs that target both Aurora A and the HP1γ pathway are emerging as tools for the treatment of diverse diseases including addiction and cancer, the new knowledge derived from the current study should be taken into consideration to predict potential side effects of these therapies on reproductive functions.
Cell lines, tissue, and gametes
Human and mouse tissue and gamete use was approved through Mayo Clinic Internal Review Board (IRB) and Institutional Animal Care and Use Committee (IACUC), respectively.
GC-1spg and GC-2spd(ts) cell lines were obtained from American Type Culture Collection and maintained according to their recommendations. GC-1spg cells were originally generated by immortalizing mouse Type B spermatogonia with SV40 that demonstrate characteristics of a stage between type B spermatogonia and primary spermatocytes . GC-2spd(ts) cells were first established by transfecting mouse spermatocytes with SV40 large T antigen [12, 13]. HP1γ (Cbx3) knockdown was achieved by lentiviral shRNA (Santa Cruz Biotechnology, Inc), according to manufacturer’s instructions with puromycin selection (2 μg/mL). Quantification of mitoses was performed through immunofluorescence with γ-tubulin. For each condition, 200 mitotic cells were analyzed. Chi-square test was used for analysis with significance of p < 0.05. To determine the percentage of cells undergoing cell division, the colorimetric Mitotic Assay Kit (Active Motif) was used according to manufacturer’s instructions with normalization for cell number by Crystal Violet staining.
Human spermatozoa were obtained from a fertile sperm donor collected through masturbation after 48 hours of abstinence. The semen specimen was processed on a single 90 % layer of Isolate™ (Irvine Scientific), centrifuged, washed and re-suspended in protein-free HTF. Murine spermatozoa were obtained from 8 week old C57BL/6 mice. Epididymi were dissected and sperm were collected via the swim up method.
Oocyte and embryo collection and culture
Four week old FVB female mice (Charles River Laboratories) were superovulated with 5 IU of intraperitoneal pregnant mare serum (NHPP) followed 48 hours later with 5 IU of intraperitoneal human chorionic gonadotropin (hCG; APP Pharmaceuticals). For oocyte collection, females were sacrificed approximately 16 hours after hCG injection and oocytes were obtained from oviducts . For embryos, females were caged individually with male CF1 mice and mating confirmed by vaginal plug. Females were sacrificed and 1-cell mouse embryos were obtained from oviducts 18 hours after hCG injection. Subsequently, embryos were cultured in 25 uL microdrops of Global media (LifeGlobal) covered with 1 mL of mineral oil (Fisher Scientific). Embryos were cultured in groups of 10 at 37 °C in 6.5 % CO2 to obtain a target pH between 7.20 and 7.30  and fixed at various time intervals. Day of sacrifice was considered Day 0, 18 hours after hCG injection, Day 0.5 (29.5hrs), Day 1 (42hrs), Day 3 (90hrs), Day 5 (138hrs).
Western blot analysis, immunofluorescence, immunohistochemistry (IHC), and confocal microscopy
Formalin-fixed mouse testis tissues were paraffin-embedded and sectioned (5 μm). Subsequently, IHC was performed as described . Dilution of the P-Ser83-HP1γ antibody [9, 10] was 1:50. Light microscopy slides were incubated with biotinylated goat anti-rabbit secondary antibody (Vector Labs), followed by HRP-streptavidin (Invitrogen), and immunoreactivity was monitored with Nova Red (Vector Labs). Negative control stains were done with incubation of secondary antibody only. Sections were counterstained with hematoxylin solution.
Fluorescence images were obtained using confocal microscopy at 40X magnification using a Zeiss LSM-780 confocal microscope and images were analyzed using ZEN software. Light microscopy images were obtained at 40X magnification using a Zeiss AxioPlan2 with AxioVision software.
Spermatozoa were processed for immunolabeling using a BioWave laboratory microwave (Ted Pella, Inc). Isolated spermatozoa were suspended in 1 % agar and fixed in 4 % paraformaldehyde + 0.1 % glutaraldehyde, dehydrated in a series of ethanol, and embedded in LR White resin. Sections (0.1 M) were mounted onto Ni mesh grids for immunolabeling. Grids were hydrated on drops of phosphate buffered saline (PBS) + 0.1 % TWEEN 20 followed by incubation with antigen retrieval solution (modified citrate, pH 6.1, Dako North America Inc.). Primary antibodies were incubated at room temperature in PBS + 0.1 % TWEEN 20 followed by secondary gold (10 nm) conjugate (BBI Research). Electron micrographs were acquired using a JEOL 1400 TEM operating at 80 kV (Jeol USA, Inc.).
mRNA isolation and RT-PCR
mRNA isolation was performed on gametes and embryos using Dynabeads mRNA DIRECT Micro Kit (Invitrogen). Oocytes and embryos at different developmental stages based on experimental protocol were placed in a lysis buffer with a concentration of 5 embryos/oocytes in 20 μL and frozen at −80 °C. cDNA synthesis was performed using the Super Script VILO cDNA synthesis Kit (Invitrogen) as per manufacturer’s protocol. Real time PCR was performed using RT2 SYBR® Green qPCR Mastermix (Qiagen) and RT2 qPCR Primers (Qiagen) on the Bio-Rad CFX96 system. Fold changes and standard error of the means (S.E.M.) were calculated using Bio-Rad CFX manager or SABioscience’s RT2 Profiler PCR Array Data Analysis software. Relative levels were normalized using ΔΔCt to GAPDH. Fold-change was calculated relative to Day 0 (Day 0 = 1).
Recombinant adenovirus and whole-genome expression profiling
Epitope-tagged (6XHis-Xpress™) wild type HP1γ, S83A or S83D as well as empty vector (EV, Ad5CMV), were generated as described . GC1-HP1γ knockdown cells were infected with recombinant adenoviruses at a multiplicity of infection (MOI) of 1:200 for 48 hours prior to RNA isolation. Global gene expression profiling was carried out at the Microarrays Facility of the Research Center of Laval University CRCHUL utilizing the Affymetrix Mouse Gene 2.0 ST arrays, as previously described . A threshold of log2 fold change ±1.25 and a p-value < 0.05 were used to select significantly altered genes. A threshold of log2 fold change between −2 and 2 and a p-value > 0.95 were used to identify unchanged genes. Data analysis, hierarchical clustering, and ontology were performed as previously described . Selected probes and their fold changes were loaded into Ingenuity Pathways Analysis Software (IPA; Ingenuity Systems) for annotation and network enrichment/analysis. Semantic relationship analysis with IPA generated significant networks of well-characterized pathways with the satisfaction of Fisher’s Exact Test. A subset of genes was validated by qPCR (Additional file 1: Fig. S1) as previously described [10, 16].
Function of HP1γ phosphorylation during mitotic cell division in male germ cells
Genetic inactivation of HP1γ in cultured male germ cell lines leads to mitotic aberrations
HP1γ regulates gene expression networks that are key for supporting normal spermatogenesis
Phosphorylation at Ser83 plays a role in HP1γ-mediated regulation of spermatogenesis-associated gene expression networks
To characterize the relationship of HP1γ phosphorylation on the regulation of genes identified by HP1γ knockdown, we performed a rescue experiment by expressing wild type HP1γ or phosphorylation mutants. Toward this end, we utilized our GC1-HP1γ knockdown cells and transduced them with adenoviral vectors expressing empty vector (EV), wild type HP1γ (WT-HP1γ), the non-phosphorylatable mutant (HP1γ-S83A), or a phosphomimetic form of Ser83-HP1γ (HP1γ-S83D) for Affymetrix whole genome gene expression analysis (Additional file 3: Table S6). Genes that were not significantly regulated by WT-HP1γ, HP1γ-S83A, or HP1γ-S83D (p > 0.95, fold change ±2, adjusted to EV expression) were compared to genes significantly altered in the presence of HP1γ knockdown (p < 0.05, fold change ±1.25). Rescue was a priori defined as a significant reversal in expression of the gene loci identified by HP1γ knockdown in the presence of either wild type or phospho-mutant HP1γ (S83A or S83D). Of the 273 genes affected by HP1γ knockdown identified in the previous experiment (Fig. 4a), 79 genes were not rescued by WT-HP1γ or either mutant (Additional file 4: Table S2), which suggests that their expression is not directly modulated by HP1γ or is an artifact of the gene knockdown. Expression of the phosphomimetic (S83D) and the non-phosphorylatable (S83A) forms rescued 77 genes (39.69 %; Additional file 4: Table S2), indicating that a significant portion of HP1γ function in these cells is dependent on phosphorylation. Notably, both mutants altered expression of a large subset of genes not identified in the knockdown rescue unique from wild type HP1γ overexpression, suggesting that mutation of the serine 83 site and altered phosphorylation status may possess profound pathway disruption effects. Additionally, 117 genes were rescued by WT-HP1γ (43 %; Additional file 4: Table S2). As the serine 83 site on the wild type HP1γ molecule is intact, the dependency of phosphorylation on the rescue of these genes is possible but indeterminate. From these data, we conclude that the expression of a subset of spermatogenesis-associated genes identified by HP1γ knockdown requires not only the expression but also the phosphorylation of this protein for their transcriptional control.
This study demonstrates the importance of HP1γ and its phosphorylated Ser83 form in gametes and early embryonic development. Here, we describe for the first time the temporal and spatial relationship of HP1γ with gametes and the preimplantation embryo. P- Ser83-HP1γ is present at the centriole and mid-piece of the mature spermatozoa and lacking at the spindle pole of the M2 oocyte. After fertilization, this protein resumes its mitotic role , as well as its euchromatic interphase localization upon mouse early embryonic genome activation at the 1 to 2-cell transition. The concerted regulation of relevant gene networks further confirms the importance of HP1γ in fertility.
HP1γ and its P-Ser83 form are localized to highly proliferating cells of the testis. HP1γ is present in both mitotic and meiotic cell populations with high levels localizing to spermatogonia and spermatocytes, whereas the P-Ser83 form is preferentially located in mitotically active spermatogonia, indicating the importance for the modification of this protein specifically during mitotic divisions. The regulation of relevant gene networks further confirms the importance of this protein and its Ser83 phosphorylation during spermatogenesis, as mitotic, meiotic, cellular proliferation, and cellular differentiation processes are dependent on HP1γ phosphorylation status. Thus, our data provide evidence that the appropriate transmission of HP1γ-mediated epigenetic signals is necessary for normal spermatogenesis and suggest that its disruption may contribute to problems with male fertility.
Using immunofluorescence and electron microscopy, we also localized P- Ser83-HP1γ to the midpiece of the spermatozoa, where it is highly concentrated in the centriole. Although murine sperm do not contribute their centrosome during fertilization , interestingly, studies in human Intra Cytoplasmic Sperm Injection (ICSI) have demonstrated that the intact axoneme along with the sperm head carrying the genetic material are required for successful fertilization . Furthermore, embryo development with this procedure and embryos resulting from injection of dissected sperm revealed chromosomal mosaicism with lack of bipolar spindle . Therefore, it would be interesting to see what role, if any, this modified form of HP1γ and its association with the centriole plays in this process.
The current study focused primarily on the pre-meiotic spermatogenic cell population, for which we observed the highest P-Ser83-HP1γ levels, by utilizing GC1, a SV40-immortalized mouse Type B spermatogonia cell line with characteristics of a stage between type B spermatogonia and primary spermatocytes. Our previous work has shown mitotic aberrations, cells with multiple centrosomes, and irregular spindle poles in somatic cells with HP1γ knockdown . Here, we confirm these findings in the GC1 cell line by demonstrating a significantly elevated number of abnormal mitoses in the knockdown group when compared with control. This protein was not present at the spindle poles of M2 oocytes that were awaiting fertilization, indicating that this may be an important spermatogenic contribution to the developing embryo, in particular, during early genome activation.
This is the first study to demonstrate the relationship of HP1γ and its P-Ser83 form in mouse oocytes and early embryonic development. We demonstrate that P-Ser83-HP1γ is not present in the euchromatin of pronuclear embryos and does not take on a mitotic localization at the spindle poles until the first or second cell division. After this transition, P-Ser83-HP1γ is found in the euchromatin of interphase nuclei. PCR of mouse oocytes and early embryos supported these findings demonstrating low transcription of HP1γ in mouse oocytes and pronuclear embryos with increased transcript levels at the 1–2 cell (Day 0.5) transition. Interestingly, the 1–2 cell mouse embryonic transition has been shown to coincide with early embryonic genome activation , suggesting that this epigenetic regulator is immediately engaged in its role in chromatin organization, as well as subsequent cell divisions.
In summary, the results of this study provide evidence that HP1γ in sperm may contribute to early embryonic mitotic divisions that occur at the time of embryonic genome activation. Furthermore, disruption of this HP1γ-Aurora Kinase pathway may contribute to spermatogenic as well as early embryonic cleavage abnormalities leading to infertility or early pregnancy loss.
This work was supported by funding, from the National Institutes of Health (grants R01 CA178627 to GL, R01 DK52913 to RU and T32CA148073 to AG), the Mayo Foundation, as well as the Mayo Clinic Center for Cell Signaling in Gastroenterology (P30DK084567) and a Career Development Award from the Mayo Clinic SPORE in Pancreatic Cancer (P50 CA102701, both to GL).
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