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Fig. 2 | BMC Developmental Biology

Fig. 2

From: The Aurora A-HP1γ pathway regulates gene expression and mitosis in cells from the sperm lineage

Fig. 2

Spatiotemporal phosphorylation and expression of HP1γ increases during early embryonic development. a. Immunofluorescence of P-S83-HP1γ in mouse oocytes and embryos. Immunofluorescence depicting P-S83-HP1γ (green), γ Tubulin (red) and DAPI DNA staining (blue). P-S83-HP1γ colocalizes to centrosomes at the time of mouse early embryonic genome activation (Day 0.5; arrow) and maintains this location through the remainder of mitotic divisions. After syngamy this protein then localizes to its euchromatic location during interphase in cells in the preimplantation embryo.b. HP1γ mRNA expression during early embryonic development. mRNA from mouse oocytes and embryos were analyzed by Q-PCR. Relative expression was normalized using ΔΔCt for HP1γ levels to GAPDH. Lowest value in each analysis (Day 0) was normalized as 1 with fold changes depicted in logarithmic units on the Y-axis. Error bars represent S.E.M. Time course of embryos post HCG injection was Day 0 (18 hrs), Day 0.5 (29.5 hrs), Day 1 (42 hrs), Day 3 (90 hrs), Day 5 (138 hrs)

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