- Research article
- Open Access
Development of the mammalian liver and ventral pancreas is dependent on GATA4
© Watt et al; licensee BioMed Central Ltd. 2007
Received: 22 January 2007
Accepted: 23 April 2007
Published: 23 April 2007
In the mouse, the parenchyma of both the liver and ventral pancreas is specified from adjacent domains of the ventral foregut endoderm. GATA4, a zinc finger transcription factor, is strongly expressed in these endodermal domains and molecular analyses have implicated GATA4 in potentiating liver gene expression during the onset of hepatogenesis. We therefore hypothesized that GATA4 has an integral role in controlling the early stages of pancreatic and liver development.
To determine whether GATA4 contributes to development of either the pancreas or liver we characterized the formation of pancreatic and hepatic tissues in embryos derived from Gata4-/- ES cells by tetraploid embryo complementation. In the absence of GATA4, development of the liver and ventral pancreas was disrupted. At embryonic day (E) 9.5, the liver bud failed to expand although, contrary to expectations, the hepatic endoderm was able to form a pseudo-stratified epithelial liver bud that expressed hepatic genes. Moreover, as we had shown previously, the embryos lacked septum transversum mesenchyme suggesting that liver defects may be cell non-autonomous. Analyses of pancreatic development revealed a complete absence of the ventral but not the dorsal pancreas in Gata4-/- embryos. Moreover, Gata6-/- embryos displayed a similar, although less dramatic phenotype, suggesting a critical role for multiple GATA factors at the earliest stages of ventral pancreas development.
This study defines integral roles for GATA factors in controlling early development of the mammalian liver and pancreas.
In the mouse, the ventral foregut endoderm differentiates to form the parenchymal components of the liver and ventral pancreas. This process begins at approximately embryonic day (E) 8.0 with the ventral foregut positioned such that a portion of it lies immediately adjacent to the cardiac mesoderm with the most ventral region distal from the heart [1, 2]. Growth factor signalling from the cardiac mesoderm and septum transversum mesenchyme specifies the underlying endoderm to adopt a hepatic fate such that by the 6–7 somite stage hepatic gene expression can be detected in the ventral foregut endoderm [3–5]. Concurrent with these events, the most distal region of the foregut endoderm starts to express pancreatic genes . Specification of these cells is dependent on their position being outside of the proximity of the hepatic inductive effects of the heart [1, 6]. Closure of the foregut pocket positions the newly specified hepatic and ventral pancreatic endoderm in close apposition to inductive mesenchyme that subsequently drives the proliferation and expansion of these organs from approximately E9.0 (reviewed in [7, 8]).
Molecular and genetic analyses have identified several transcription factors central to these processes including forkhead box proteins A1 and A2 (FOXA1 and FOXA2), the homeodomain transcription factors HEX and PROX1, and the PTF1a subunit of the PTF1 transcription factor (reviewed in [7–9]). Careful analysis of transcription factor function in the foregut endoderm has, in turn, helped define the fundamental contributions that cell proliferation and transient tissue interactions make to both hepatic and pancreatic development (for reviews see [10–12]).
The GATA family of zinc finger transcription factors have also been shown to be involved in the differentiation of the endoderm in several evolutionarily diverse organisms [13–15]. In the mouse, GATA4, 5 and 6 are expressed in the definitive endoderm and its derivatives, and are required to modulate endodermal gene expression [16–21]. Moreover, we have demonstrated previously that hepatic development is blocked at the primary liver bud stage in embryos derived from Gata6-/- ES cells by tetraploid complementation . In these embryos, low levels of hepatic mRNAs were detected in the foregut endoderm at the 6–7 somite stage suggesting that Gata6 is not essential for hepatic specification. However, the hepatic endoderm subsequently failed to expand and commit to a normal liver developmental program .
GATA4 was the first GATA factor to be implicated in development of the ventral foregut. In vivo footprinting analyses revealed that GATA4 occupied the hepatic Albumin (Alb1) enhancer in ventral foregut endoderm prior to the onset of hepatic gene expression . The ability of GATA4, in conjunction with FOXA2, to reposition nucleosomes around this enhancer has lead to the hypothesis that GATA4 potentiates hepatic gene expression. In this model, GATA4 acts with FoxA to ensure that hepatic transcriptional regulatory elements are structurally capable of interacting with liver-expressed transcriptional activators whose own expression could be induced by paracrine signals from surrounding tissues [23–25]. From a developmental perspective, GATA4 would act as a 'pioneer' transcription factor that, along with FOXA, would help define the competency of the endoderm to adopt a hepatic fate.
Further support for GATA4 contributing to hepatic and pancreatic development also comes from studies in zebrafish . In these studies, morpholino-mediated depletion of zfGATA4 resulted in defective development of multiple organs including the liver and pancreas. With regard to development of the liver, depletion of either zfGATA4 or zfGATA6 resulted in a failure of the liver lineage to expand although hepatic specification was intact. However, when both zfGATA4 and zfGATA6 were simultaneously depleted there was no indication that the liver had initiated development, suggesting that in zebrafish zfGATA4 and zfGATA6 had redundant roles during the onset of hepatic development but had independent roles during organogenesis of the liver.
The phenotype observed in zebrafish depleted of zfGATA6 closely resembles that described for mouse embryos generated from Gata6-/- ES cells, implying that the role for GATA6 is evolutionarily conserved between fish and mammals [22, 26]. However, there has been debate over whether zfGATA5 is in fact the functional homolog of mouse GATA4 , and so it remained an open question as to whether GATA4 is required for development of the mammalian liver. Development of Gata4-/- mouse embryos arrests prior to the onset of pancreatic and liver specification due to deficiencies in the differentiation of the extraembryonic endoderm [28, 29]. However, as is the case for Gata6-/- embryos, this early embryonic lethality can be circumvented by providing Gata4-/- embryos with a Gata4+/+ extraembryonic endoderm by tetraploid embryo complementation [30, 31]. We therefore used this approach to generate post-gastrulation stage embryos from Gata4-/- ES cells in order to definitively determine whether GATA4 controls development of the mammalian pancreas or liver. Examination of such Gata4-/- ES cell-derived embryos revealed that GATA4 is necessary for expansion of the primary hepatic rudiment and is required for the onset of ventral pancreatic development but dispensable for formation of the dorsal pancreas.
GATA4 is expressed in multiple tissues with roles in development of the pancreas and liver
GATA4 is necessary for expansion of the liver bud
Although GATA4 is expressed in the early developing endoderm, the level of GATA4 protein drops to levels that are undetectable by immunohistochemistry at around the time the liver bud expands and the cells delaminate. This implies that the failure of the liver bud to expand and invade the surrounding mesenchyme is likely to reflect a non-cell autonomous role for GATA4 during hepatogenesis. Consistent with this proposal, GATA4 was also identified in the cardiac mesoderm, septum transversum mesenchyme and endothelium surrounding the liver bud, all of which are crucial for the development of the hepatic domain [5, 37]. We have previously shown that although loss of GATA4 affects development of the ventricular cardiomyocytes, there is relatively little impact on cardiac gene expression possibly due to compensation by GATA6 . A comparison of transverse sections from Gata4+/- and Gata4-/- embryos reveals that the septum transversum mesenchyme, which lies adjacent to the presumptive liver bud in control embryos, is absent in Gata4-/- embryos (Fig. 2A,B). The apparent absence of this mesenchyme was confirmed by immunohistochemical staining (Fig. 2G,H) for WT1 . These data are consistent with our previously published analyses of the septum transversum and proepicardium in Gata4-/- embryos . Next, we examined whether endothelial cells, which normally line the ventro-lateral surface of the hepatic epithelium, were present in Gata4-/- embryos. We were able to detect platelet endothelial cell adhesion molecule (PECAM) positive cells on the surface of the liver bud in Gata4+/- and Gata4-/- embryos (Fig. 2I,J) suggesting that the presence of endothelial cells alone are insufficient to induce the outgrowth of the hepatic diverticulum. Although it is possible that GATA4 specifically regulates expression of an essential signalling molecule from the endothelium or cardiomycoytes, we believe it more likely that the absence of the septum transversum mesenchyme in Gata4 null embryos accounts for the failure of the outgrowth of the hepatic endoderm in these embryos.
GATA4 is essential for formation of the ventral pancreatic bud
The transcription factor PDX1 is among the earliest expressed proteins in both the ventral and dorsal pancreatic rudiments and provides one of the few markers that define the ventral pancreas at this developmental stage [6, 39]. As expected, we were able to detect PDX1 expression by immunohistochemistry in the ventral pancreas of Gata4+/- embryos at E9.5 (Fig. 4C). In contrast, PDX1 expression was undetectable in the equivalent endodermal region in Gata4-/- embryos (Fig. 4D). Indeed, we failed to observe any PDX1 expression in the ventral aspect of the entire gut endoderm (data not shown). However, we were able to detect PDX1, as well as a second pancreatic protein HB9, in the dorsal pancreatic domain of both control and mutant embryos (Fig. 4E,F,G) implying that the loss of GATA4 specifically affects development of the ventral pancreas.
The pancreatic phenotype described above exhibits many similarities to the ventral pancreatic defects observed in Hex null embryos [6, 33]. The hepatic and ventral pancreatic lineages are specified from two distinct domains of the ventral foregut endoderm. Signals from the overlying cardiac mesoderm instruct the more dorsal endoderm to assume a hepatic fate . The ventral-most endoderm is beyond the influence of these signals and as a result adopts a pancreatic fate. In Hex null embryos, reduced proliferation of the ventral foregut endoderm results in a failure to elaborate endoderm beyond the hepatic inductive influence of the cardiac mesoderm. Consequently, the ventral pancreas is not specified, with PDX1 expression absent in E9.5 Hex null embryos . To examine whether the ventral pancreatic defects observed in Gata4 null embryos are a consequence of a similar mechanism, we examined the development of the ventral foregut endoderm at around the 6 somite stage. In Hex null embryos, the failure of the ventral foregut endoderm to proliferate is characterised by a dorsal ingression of the visceral extraembryonic endoderm . Using HNF4α as a marker that specifically identifies the extraembrynoic visceral endoderm at this developmental stage, we found that the positioning of the visceral extraembryonic endoderm relative to the ventral foregut and cardiac mesoderm between Gata4+/- or Gata4-/- embryos was indistinguishable. These data suggest that the failure of ventral pancreas specification in Gata4 null embryos is via a mechanism distinct from that observed in Hex-/- embryos. This interpretation is supported by RT-PCR analysis of the ventral endoderm that shows comparable levels of Hex mRNA expression between Gata4+/- and Gata4-/- embryos (Fig. 3).
The generation of embryos from Gata4 null ES cells by tetraploid complementation has allowed us to examine the development of the mammalian liver and pancreas from the ventral foregut endoderm in the absence of either GATA4 or GATA6. Studies on the transcriptional control of the Alb1 gene have implicated GATA transcription factors, and in particular GATA4, as being crucial during the early development of the liver. In the ventral foregut endoderm, GATA4, along with FOXA2, has been shown to bind to Alb1 transcriptional regulatory elements prior to the onset of hepatic gene expression [3, 23]. The ability of GATA4 and FOXA2 to alter chromatin structure has led to the hypothesis that these two proteins act to potentiate hepatic gene expression by preparing the promoters and enhancers of hepatic genes for the action of transcriptional activators . While our studies support a role for GATA4 and GATA6 during liver development, it is clear that the contribution of these two GATA factors is complex, with both factors acting dynamically at distinct stages of hepatic development and likely having both cell autonomous and non-autonomous roles.
The expression pattern of GATA4 points to a transient function for this gene during the earliest stages of hepatic development. This is compatible with GATA4 acting as a pioneer factor within the ventral endoderm during liver development. However, our finding that expression of liver genes is relatively unaffected in the hepatic endoderm between the 6–12 somite stage and that formation of a pseudostratified epithelial bud can be detected in Gata4 null embryos suggests that GATA4 is not essential for the onset of a hepatic developmental program. However, since both GATA4 and GATA6 are expressed in the ventral endoderm and both bind identical regulatory elements in target promoters, it seems reasonable to consider that the function of these two factors overlaps to facilitate the expression of liver genes during hepatic specification. GATA6 has been predicted to compensate for GATA4 function in a number of other studies, and Gata4+/-Gata6+/- mouse embryos arrest during midgestation stages of development due to cardiovascular defects [29–31, 40]. Moreover, direct evidence supporting the proposal that GATA4 and GATA6 have functionally redundant roles during specification of the liver that are evolutionarily conserved come from studies in zebrafish . Holtzinger and Evans have demonstrated that depletion of either zfGATA4 or zfGATA6 prevents expansion of the primary liver bud but does not block specification whereas depletion of both factors results in the absence of any detectable hepatic endoderm.
Although the specification of the hepatic lineage and initial formation of the liver bud appears relatively normal in Gata4 null mouse embryos, the liver bud fails to expand. Based on the observation that GATA4 is undetectable in the hepatic endoderm as it expands into the adjacent septum transversum mesenchyme, it appears unlikely that GATA4 has a cell autonomous role in this process in mammals. Tissue explant studies in chick and mouse have shown that an interaction with the septum transversum mesenchyme is crucial for the expansion of the liver bud, an effect that can be mediated by the growth factors BMP2 and 4 [5, 41, 42]. In Gata4 null embryos, the absence of septum transversum mesenchyme adjacent to the liver bud would, therefore, be predicted to result in a failure in growth factor signalling necessary to drive expansion of the liver diverticulum. This in turn would imply that the failure of the Gata4-/- liver bud to expand reflects a secondary effect caused by the failure of the septum transversum mesenchyme to develop rather than a cell autonomous requirement for GATA4 within the hepatic endoderm.
The identification of defects in ventral pancreas development in Gata4 and Gata6 null embryos reveals roles for GATA factors in this tissue. Specification of the ventral pancreas, as defined by expression of Pdx1, can be detected at the 7–8 somite stage in the ventral-most foregut endoderm. Specification of the ventral pancreas is dependent on the endoderm being positioned beyond the influence of FGF signals that originate from the cardiac mesoderm that specify the liver primordium . Examination of Gata4 null embryos at the 20 somite stage implied a complete absence of ventral pancreatic tissue that expresses PDX1. In Gata6 null embryos, although some PDX1 positive cells could be detected, their abundance was reduced. However, in contrast to the ventral pancreas, we were able to detect PDX1 and Hb9 in the dorsal pancreatic rudiment in Gata4 or Gata6 null embryos. This presumably reflects the fact that the tissue interactions and growth factor requirements necessary for development of the ventral pancreas are distinct from those of the dorsal pancreas .
The absence of a ventral pancreas in Gata4 null embryos is similar to that of embryos that lack the transcription factor Hex [6, 44]. In the absence of Hex, the morphology of the ventral foregut endoderm is compromised such that the subset of cells normally specified to become pancreas failed to extend beyond the inductive affect of the cardiac mesoderm. Consequently, the ventral pancreas was absent in these embryos as the ventral foregut endoderm followed an alternate fate . However, analyses of explant cultures of endoderm revealed that Hex-/-foregut endoderm retained the capacity to express pancreatic genes demonstrating that the absence of the ventral pancreas was not cell autonomous. Although, like Hex-/- embryos, loss of GATA4 prevents formation of the ventral pancreas, the mechanism seems to be distinct because in contrast to Hex-/- embryos morphogenesis of the foregut appears to occur normally in Gata4-/- embryos. The possibility therefore exists that the GATA factors regulate ventral pancreatic development by potentiating pancreatic gene expression as has been proposed for the liver. This possibility is supported by a recent study showing that the Glucagon promoter can be directly activated by GATA4 in cell culture . Although both GATA4 and GATA6 are expressed (data not shown) in the mesenchyme adjacent to the pancreatic endoderm and could, therefore, have cell non-autonomous roles during pancreatic development, studies using transgenic mouse embryos are consistent with a requirement for the GATA factors in the endoderm . Decker et al generated transgenic mouse embryos that expressed GATA4 and GATA6 proteins fused to the Engrailed repressor domain within the developing pancreatic rudiments of transgenic mouse embryos using a Pdx1 promoter. Although the mechanism of action of GATA-Engrailed fusion proteins is likely to be complex, examination of five independent E17.5 GATA6-Engrailed transgenic embryos revealed that the pancreas was undetectable in three, while two displayed a severe disruption to pancreatic morphology. Similar expression of a GATA4-Engrailed fusion was less dramatic with one out of eight embryos displaying an abnormal pancreatic morphology.
We have identified GATA4 and GATA6 as key transcription factors that act to control the development of the hepatic and pancreatic lineages from the ventral foregut endoderm. A comparison of the expression profiles of these factors implies that these two factors have a complex functional relationship during this developmental regulation that likely involves both cell autonomous and non-autonomous roles. The precise cell type-specific contribution made by these factors during pancreatic and liver development of these tissues should be resolved by the endodermal or mesenchymal specific deletion of GATA4 and GATA6 by Cre-loxP technologies using available conditional alleles for Gata4 and Gata6 [31, 45]. In addition, it is likely that these factors have both redundant and specific functions during development, which could be resolved by generating embryos from ES cells that are null for both GATA4 and GATA6.
Generation of ES cell-derived embryos
The generation of Gata4-/- and Gata6-/- ES cells and formation of ES-cell derived embryos by complementation with tetraploid embryos has been reported previously [22, 31, 46]. The Medical College of Wisconsin's Animal Care Committee approved all animal procedures used in this study. PMSG used in super ovulation was obtained from A.F. Parlow at the National Hormone and Peptide Program.
Histochemistry and Immunohistochemistry
Immunohistochemistry was performed using antigen retrieval as described previously  using the following antibodies: GATA4 and HNF4α (Santa Cruz Biotechnology), Wilms' Tumor (WT1) (Cell Marque), Platelet endothelial cell adhesion molecule (PECAM, CD31, BD Pharmingen) and PDX1 (gift of Dr Christopher Wright, Vanderbilt University, Tennessee).
Embryos were dehydrated by alcohol series and embedded in paraffin and sections were cut at 7 μm. Slides were subsequently processed for in situ hybridization with P33-labeled anti-sense RNA probes as described previously [32, 48]. Experimental and control images were processed identically using Adobe Photoshop.
RT-PCR was carried out using total RNA isolated (RNeasy Mini Prep Kit, Qiagen) from ventral endoderm dissected from embryos as described previously  using the following primers: Hprt1; agcgcaagttgaatctgc, agcgacaatctaccagag, Hnf4α ; cttccttcttcatgccag, acacgtccccatctgaag, Alb1; ccccactagcctctggcaaaat, cttaaaccgatgggcgatctcact, Afp; tcgtattccaacaggagg, aggcttttgcttcaccag. Gata6, atggcgtagaaatgctgagg, tgaggtggtcgcttgtgtag; Gata4, tggccgacgtgggagcat, cggcgggaagcggacag, Rbp, atccagtggtcatcgtttcctcgct, gaacttcgacaaggctcgtttctctgg; Ttr, ctcaccacagatgagaag, ggctgagtctctcaattc, Foxa2; actggagcagctactacg, cccacataggatgacatg, Hex; cccgctcacccgacgcccttcta, aacctcacttgaccgcctttccttttgt.
- Deutsch G, Jung J, Zheng M, Lora J, Zaret KS: A bipotential precursor population for pancreas and liver within the embryonic endoderm. Development. 2001, 128 (6): 871-881.PubMedGoogle Scholar
- Tremblay KD, Zaret KS: Distinct populations of endoderm cells converge to generate the embryonic liver bud and ventral foregut tissues. Dev Biol. 2005, 280 (1): 87-99. 10.1016/j.ydbio.2005.01.003.View ArticlePubMedGoogle Scholar
- Gualdi R, Bossard P, Zheng M, Hamada Y, Coleman JR, Zaret KS: Hepatic specification of the gut endoderm in vitro: cell signaling and transcriptional control. Genes Dev. 1996, 10: 1670-1682.View ArticlePubMedGoogle Scholar
- Jung J, Zheng M, Goldfarb M, Zaret KS: Initiation of mammalian liver development from endoderm by fibroblast growth factors. Science. 1999, 284 (5422): 1998-2003. 10.1126/science.284.5422.1998.View ArticlePubMedGoogle Scholar
- Rossi JM, Dunn RN, Hogan BLM, Zaret KS: Distinct mesodermal signals, including BMPs from septum transversum mesenchyme, are required in combination for hepatogenesis from the endoderm. Genes Dev. 2001, 15 (15):Google Scholar
- Bort R, Martinez-Barbera JP, Beddington RS, Zaret KS: Hex homeobox gene-dependent tissue positioning is required for organogenesis of the ventral pancreas. Development. 2004, 131 (4): 797-806. 10.1242/dev.00965.View ArticlePubMedGoogle Scholar
- Duncan SA: Mechanisms controlling early development of the liver. Mech Dev. 2003, 120 (1): 19-33. 10.1016/S0925-4773(02)00328-3.View ArticlePubMedGoogle Scholar
- Zaret KS: Regulatory phases of early liver development: paradigms of organogenesis. Nat Rev Genet. 2002, 3 (7): 499-512. 10.1038/nrg837.View ArticlePubMedGoogle Scholar
- Bort R, Zaret K: Paths to the pancreas. Nat Genet. 2002, 32 (1): 85-86. 10.1038/ng0902-85.View ArticlePubMedGoogle Scholar
- Wilson ME, Scheel D, German MS: Gene expression cascades in pancreatic development. Mech Dev. 2003, 120 (1): 65-80. 10.1016/S0925-4773(02)00333-7.View ArticlePubMedGoogle Scholar
- Lemaigre F, Zaret KS: Liver development update: new embryo models, cell lineage control, and morphogenesis. Curr Opin Genet Dev. 2004, 14 (5): 582-590. 10.1016/j.gde.2004.08.004.View ArticlePubMedGoogle Scholar
- Zhao R, Duncan SA: Embryonic development of the liver. Hepatology. 2005, 41 (5): 956-967. 10.1002/hep.20691.View ArticlePubMedGoogle Scholar
- Reiter JF, Kikuchi Y, Stainier DY: Multiple roles for Gata5 in zebrafish endoderm formation. Development. 2001, 128 (1): 125-135.PubMedGoogle Scholar
- Zhu J, Fukushige T, McGhee JD, Rothman JH: Reprogramming of early embryonic blastomeres into endodermal progenitors by a Caenorhabditis elegans GATA factor. Genes Dev. 1998, 12 (24): 3809-3814.PubMed CentralView ArticlePubMedGoogle Scholar
- Rehorn KP, Thelen H, Michelson AM, Reuter R: A molecular aspect of hematopoiesis and endoderm development common to vertebrates and Drosophila. Development. 1996, 122 (12): 4023-4031.PubMedGoogle Scholar
- Decker K, Goldman DC, Grasch CL, Sussel L: Gata6 is an important regulator of mouse pancreas development. Dev Biol. 2006, 298 (2): 415-429. 10.1016/j.ydbio.2006.06.046.PubMed CentralView ArticlePubMedGoogle Scholar
- Gao X, Sedgwick T, Shi YB, Evans T: Distinct functions are implicated for the GATA-4, -5, and -6 transcription factors in the regulation of intestine epithelial cell differentiation. Mol Cell Biol. 1998, 18 (5): 2901-2911.PubMed CentralView ArticlePubMedGoogle Scholar
- Her GM, Yeh YH, Wu JL: Functional conserved elements mediate intestinal-type fatty acid binding protein (I-FABP) expression in the gut epithelia of zebrafish larvae. Dev Dyn. 2004, 230 (4): 734-742. 10.1002/dvdy.20081.View ArticlePubMedGoogle Scholar
- Molkentin JD: The zinc finger-containing transcription factors GATA-4, -5, and -6. Ubiquitously expressed regulators of tissue-specific gene expression. J Biol Chem. 2000, 275 (50): 38949-38952. 10.1074/jbc.R000029200.View ArticlePubMedGoogle Scholar
- Nemer G, Nemer M: Transcriptional activation of BMP-4 and regulation of mammalian organogenesis by GATA-4 and -6. Dev Biol. 2003, 254 (1): 131-148. 10.1016/S0012-1606(02)00026-X.View ArticlePubMedGoogle Scholar
- Ritz-Laser B, Mamin A, Brun T, Avril I, Schwitzgebel VM, Philippe J: The zinc finger-containing transcription factor Gata-4 is expressed in the developing endocrine pancreas and activates glucagon gene expression. Mol Endocrinol. 2005, 19 (3): 759-770. 10.1210/me.2004-0051.View ArticlePubMedGoogle Scholar
- Zhao R, Watt AJ, Li J, Luebke-Wheeler J, Morrisey EE, Duncan SA: GATA6 is essential for embryonic development of the liver but dispensable for early heart formation. Mol Cell Biol. 2005, 25 (7): 2622-2631. 10.1128/MCB.25.7.2622-2631.2005.PubMed CentralView ArticlePubMedGoogle Scholar
- Bossard P, Zaret KS: GATA transcription factors as potentiators of gut endoderm differentiation. Development. 1998, 125 (24): 4909-4917.PubMedGoogle Scholar
- Cirillo LA, Lin FR, Cuesta I, Friedman D, Jarnik M, Zaret KS: Opening of compacted chromatin by early developmental transcription factors HNF3 (FoxA) and GATA-4. Mol Cell. 2002, 9 (2): 279-289. 10.1016/S1097-2765(02)00459-8.View ArticlePubMedGoogle Scholar
- Cirillo LA, Zaret KS: An early developmental transcription factor complex that is more stable on nucleosome core particles than on free DNA. Mol Cell. 1999, 4 (6): 961-969. 10.1016/S1097-2765(00)80225-7.View ArticlePubMedGoogle Scholar
- Holtzinger A, Evans T: Gata4 regulates the formation of multiple organs. Development. 2005, 132 (17): 4005-4014. 10.1242/dev.01978.View ArticlePubMedGoogle Scholar
- Wallace KN, Pack M: Unique and conserved aspects of gut development in zebrafish. Dev Biol. 2003, 255 (1): 12-29. 10.1016/S0012-1606(02)00034-9.View ArticlePubMedGoogle Scholar
- Kuo CT, Morrisey EE, Anandappa R, Sigrist K, Lu MM, Parmacek MS, Soudais C, Leiden JM: GATA4 transcription factor is required for ventral morphogenesis and heart tube formation. Genes Dev. 1997, 11 (8): 1048-1060.View ArticlePubMedGoogle Scholar
- Molkentin JD, Lin Q, Duncan SA, Olson EN: Requirement of the transcription factor GATA4 for heart tube formation and ventral morphogenesis. Genes Dev. 1997, 11 (8): 1061-1072.View ArticlePubMedGoogle Scholar
- Narita N, Bielinska M, Wilson DB: Wild-type endoderm abrogates the ventral developmental defects associated with GATA-4 deficiency in the mouse. Dev Biol. 1997, 189 (2): 270-274. 10.1006/dbio.1997.8684.View ArticlePubMedGoogle Scholar
- Watt AJ, Battle MA, Li J, Duncan SA: GATA4 is essential for formation of the proepicardium and regulates cardiogenesis. Proc Natl Acad Sci U S A. 2004, 101 ((34)): 12573-12578. 10.1073/pnas.0400752101.PubMed CentralView ArticlePubMedGoogle Scholar
- Duncan SA, Manova K, Chen WS, Hoodless P, Weinstein DC, Bachvarova RF, Darnell JE: Expression of transcription factor HNF-4 in the extraembryonic endoderm, gut, and nephrogenic tissue of the developing mouse embryo: HNF-4 is a marker for primary endoderm in the implanting blastocyst. Proc Natl Acad Sci USA. 1994, 91: 7598-7602. 10.1073/pnas.91.16.7598.PubMed CentralView ArticlePubMedGoogle Scholar
- Bort R, Signore M, Tremblay K, Martinez Barbera JP, Zaret KS: Hex homeobox gene controls the transition of the endoderm to a pseudostratified, cell emergent epithelium for liver bud development. Dev Biol. 2006, 290 (1): 44-56. 10.1016/j.ydbio.2005.11.006.View ArticlePubMedGoogle Scholar
- Cascio S, Zaret KS: Hepatocyte differentiation initiates during endodermal-mesenchymal interactions prior to liver formation. Development. 1991, 113: 217-225.PubMedGoogle Scholar
- Garrison WD, Battle MA, Yang C, Kaestner KH, Sladek FM, Duncan SA: Hepatocyte nuclear factor 4alpha is essential for embryonic development of the mouse colon. Gastroenterology. 2006, 130 (4): 1207-1220. 10.1053/j.gastro.2006.01.003.PubMed CentralView ArticlePubMedGoogle Scholar
- Meehan RR, Barlow DP, Hill RE, Hogan BLM, Hastie ND: Pattern of serum protein gene expression in the mouse visceral yolk sac and foetal liver. EMBO J. 1984, 3 (8): 1881-1885.PubMed CentralPubMedGoogle Scholar
- Matsumoto K, Yoshitomi H, Rossant J, Zaret KS: Liver organogenesis promoted by endothelial cells prior to vascular function. Science. 2001, 294 (5542): 559-563. 10.1126/science.1063889.View ArticlePubMedGoogle Scholar
- Moore AW, Schedl A, McInnes L, Doyle M, Hecksher-Sorensen J, Hastie ND: YAC transgenic analysis reveals Wilms' tumour 1 gene activity in the proliferating coelomic epithelium, developing diaphragm and limb. Mech Dev. 1998, 79 (1-2): 169-184. 10.1016/S0925-4773(98)00188-9.View ArticlePubMedGoogle Scholar
- Gu G, Dubauskaite J, Melton DA: Direct evidence for the pancreatic lineage: NGN3+ cells are islet progenitors and are distinct from duct progenitors. Development. 2002, 129 (10): 2447-2457.PubMedGoogle Scholar
- Xin M, Davis CA, Molkentin JD, Lien CL, Duncan SA, Richardson JA, Olson EN: A threshold of GATA4 and GATA6 expression is required for cardiovascular development. Proc Natl Acad Sci U S A. 2006, 103 (30): 11189-11194. 10.1073/pnas.0604604103.PubMed CentralView ArticlePubMedGoogle Scholar
- Fukuda-Taira S: Hepatic induction in the avian embryo: specificity of reactive endoderm and inductive mesoderm. J Embryol Exp Morphol. 1981, 63: 111-125.PubMedGoogle Scholar
- LeDouarin NM: An experimental analysis of liver development. Med Biol. 1975, 53: 427-455.Google Scholar
- Edlund H: Pancreatic organogenesis--developmental mechanisms and implications for therapy. Nat Rev Genet. 2002, 3 (7): 524-532. 10.1038/nrg841.View ArticlePubMedGoogle Scholar
- Martinez Barbera JP, Clements M, Thomas P, Rodriguez T, Meloy D, Kioussis D, Beddington RS: The homeobox gene Hex is required in definitive endodermal tissues for normal forebrain, liver and thyroid formation. Development. 2000, 127 (11): 2433-2445.PubMedGoogle Scholar
- Sodhi CP, Li J, Duncan SA: Generation of mice harbouring a conditional loss-of-function allele of Gata6. BMC Dev Biol. 2006, 6 (1): 19-10.1186/1471-213X-6-19.PubMed CentralView ArticlePubMedGoogle Scholar
- Duncan SA, Nagy A, Chan W: Murine gastrulation requires HNF-4 regulated gene expression in the visceral endoderm: tetraploid rescue of HNF-4-/- embryos. Development. 1997, 124: 279-287.PubMedGoogle Scholar
- Parviz F, Matullo C, Garrison WD, Savatski L, Adamson JW, Ning G, Kaestner KH, Rossi JM, Zaret KS, Duncan SA: Hepatocyte nuclear factor 4alpha controls the development of a hepatic epithelium and liver morphogenesis. Nat Genet. 2003, 34 (3): 292-296. 10.1038/ng1175.View ArticlePubMedGoogle Scholar
- Rausa FM, Ye H, Lim L, Duncan SA, Costa RH: In situ hybridization with 33P-labeled RNA probes for determination of cellular expression patterns of liver transcription factors in mouse embryos [published erratum appears in Methods 1998 Nov;16(3):359-60]. Methods. 1998, 16 (1): 29-41. 10.1006/meth.1998.0642.View ArticlePubMedGoogle Scholar
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