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Figure 3 | BMC Developmental Biology

Figure 3

From: Development of the mammalian liver and ventral pancreas is dependent on GATA4

Figure 3

Analysis of hepatic gene expression in Gata4-/- embryos. RT-PCR was performed on RNA from the foregut endoderm of 4 individual Gata4+/- embryos (lanes 1–4) and 7 individual Gata4-/- embryos (lanes 5–11). The somite number of each embryo is given above each lane. Gata4-/- ventral endoderm does not express Gata4 (lanes 5–11) confirming the genotype of these embryos and the clean dissection of the foregut endoderm from wild type extraembryonic visceral endoderm. Amplification of Hprt was used to control for loading and amplification of samples lacking reverse transcriptase (-RT) or cDNA (lane 12) confirmed the absence of contaminating DNA.

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