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nfi-1 affects behavior and life-span in C. elegansbut is not essential for DNA replication or survival

BMC Developmental Biology volume 5, Article number: 24 (2005) Cite this article

Abstract

Background

The Nuclear Factor I (one) (NFI) family of transcription/replication factors plays essential roles in mammalian gene expression and development and in adenovirus DNA replication. Because of its role in viral DNA replication NFI has long been suspected to function in host DNA synthesis. Determining the requirement for NFI proteins in mammalian DNA replication is complicated by the presence of 4 NFI genes in mice and humans. Loss of individual NFI genes in mice cause defects in brain, lung and tooth development, but the presence of 4 homologous NFI genes raises the issue of redundant roles for NFI genes in DNA replication. No NFI genes are present in bacteria, fungi or plants. However single NFI genes are present in several simple animals including Drosophila and C. elegans, making it possible to test for a requirement for NFI in multicellular eukaryotic DNA replication and development. Here we assess the functions of the single nfi-1 gene in C. elegans.

Results

C. elegans NFI protein (CeNFI) binds specifically to the same NFI-binding site recognized by vertebrate NFIs. nfi-1 encodes alternatively-spliced, maternally-inherited transcripts that are expressed at the single cell stage, during embryogenesis, and in adult muscles, neurons and gut cells. Worms lacking nfi-1 survive but have defects in movement, pharyngeal pumping and egg-laying and have a reduced life-span. Expression of the muscle gene Ce titin is decreased in nfi-1 mutant worms.

Conclusion

NFI gene function is not needed for survival in C. elegans and thus NFI is likely not essential for DNA replication in multi-cellular eukaryotes. The multiple defects in motility, egg-laying, pharyngeal pumping, and reduced lifespan indicate that NFI is important for these processes. Reduction in Ce titin expression could affect muscle function in multiple tissues. The phenotype of nfi-1 null worms indicates that NFI functions in multiple developmental and behavioral systems in C. elegans, likely regulating genes that function in motility, egg-laying, pharyngeal pumping and lifespan maintenance.

Background

We are studying the role of the highly conserved Nuclear Factor I (NFI) family of site-specific DNA-binding proteins in metazoan development. NFI was first identified as a protein from human HeLa cells required for efficient adenovirus (Ad) DNA synthesis in vitro [1]. A binding site for NFI proteins in the Ad origin of replication is essential for viral replication both in vitro and in vivo [1–5]. These and other studies suggested that NFI proteins may function in the replication of host cell DNA [6, 7]. However there is no direct evidence to support or refute a role for NFI proteins in host DNA synthesis. In contrast, many studies have identified NFI binding sites in the promoter and distal control regions of cellular genes, and deletion analysis of sites shows that NFI proteins are important for gene expression in a variety of cell types [8, 9].

Four conserved NFI genes are present in vertebrates (NFIA, NFIB, NFIC and NFIX in humans; Nfia, Nfib, Nfic and Nfix in mice) [10–13]. Single NFI genes have been identified in the simple metazoans Amphioxus, C. elegans, and Drosophila [14, 15], but no NFI genes are present in fungi, Arabidopsis or any sequenced prokaryotic genome. Thus the NFI gene family arose during early metazoan evolution and appears to be present only in multicellular animals. NFI proteins bind as either homo- or heterodimers [16, 17] to the symmetric consensus sequence TTGGC(N5)GCCAA in duplex DNA [18, 19]. NFI proteins also bind with lower affinity to sites containing a single TTGGC motif [20, 21]. NFI homo- and heterodimers bind to the same sites with similar affinities, making it difficult to determine which family members play essential roles at specific cellular promoters. In addition, the 4 NFI genes in vertebrates are alternatively spliced [16, 22] and are expressed in specific but widely overlapping patterns during embryogenesis and adult life [13] making it difficult to assess the role of specific NFI genes in development. The role of the NFI genes in development is of particular interest because binding sites for NFI proteins have been identified in genes expressed in virtually every tissue and organ system of vertebrates including brain [23, 24], muscle [25] and other tissues. NFI proteins have also been implicated in the control of gene expression by a number of hormones and physiological modulators including glucocorticoids [26, 27], insulin [28, 29], TGFβ [30, 31] and others.

To assess the roles of NFI genes in development we began a genetic analysis of the NFI genes in mice and C. elegans. Disruption of Nfia results in neurological defects including agenesis of the corpus callosum, loss of midline glial cells [32], hydrocephalus and perinatal lethality [33]. Disruption of Nfic causes defects in tooth development [34] while loss of Nfib results in perinatal lethality due to defects in lung maturation [35, 36]. In each knockout defects are seen in the presence of the other three vertebrate NFI genes, suggesting that the 4 mouse NFI genes each have essential roles in development. However the presence of 4 NFI genes in mice has made it impossible to test whether NFI activity per se is essential for survival.

Since C. elegans has only one NFI gene (nfi-1), it provides an ideal system to assess the role of NFI in DNA replication and simple animal development. We show here that C. elegans nfi-1 and its products share many properties with their vertebrate homologs including similar DNA-binding activity, alternative splicing and expression during embryogenesis and in adult tissues. Loss of nfi-1 results in viable worms with multiple defects in locomotion, pharyngeal pumping and egg-laying, and a shortened life-span. Thus, nfi-1 is non-essential for survival but plays an important role in C. elegans physiology.

Results

Alternative splicing and promoter of the nfi-1gene

The C. elegans nuclear factor I gene (nfi-1) was first identified by the C. elegans sequencing consortium by its homology to mammalian NFI genes [14]. Only a single NFI gene is present in the C. elegans genome (Fig. 1A) while vertebrates possess 4 NFI genes. To confirm the structure of the nfi-1 gene, we obtained cDNAs from the Kohara and TIGR libraries and made primers within predicted exons for production of cDNAs from RNA of adult worms and purified eggs. These cDNAs confirmed the presence of 14 exons and showed alternative splicing of exons 2 and 13 (Fig. 1A). The differential splicing pattern of nfi-1 indicates that different isoforms may be expressed in different cell types. Primary transcripts from most C. elegans genes are trans-spliced to SL1 leader sequences and detection of SL1-linked transcripts is frequently used to assess the initially transcribed exons of genes [37]. We confirmed the start site of the gene using PCR with an SL1 primer for SL1-containing transcripts and a primer in exon 2.

Figure 1
figure 1

A) Alternatively spliced products of the nfi-1 gene. The nfi-1 gene is shown as a line with exons as numbered solid boxes and alternatively spliced exons as gray boxes. Arrows show the direction of transcription. Below the line are cDNAs from the Kohara and TIGR libraries; the vertical bar indicates the 5' end of the cDNA. In yK213C10 the letters a and b above exon 2 denote that it is alternatively spliced generating 2a and 2b and the arrow on the right denote undetermined sequence in the cDNA. The asterisk (*) on yk42f10 denotes an alternative 3'splice acceptor site in exon 13 used in yk42f10 but not in CEESQ09. Below are depictions of cDNAs obtained by RT-PCR from total and polyA+ RNA of whole worms or isolated eggs using nfi-1 exon-specific primers (RT-PCR nfi-1 primers). Lastly we show cDNA obtained by PCR using SL1 primer and nfi-1 exon-specific primers (RT-PCR SL1-linked products). Some of the alternatively spliced cDNAs have been described previously [14]. B) Comparison of CeNFI-DBD with the consensus mouse NFI DBD.The sequence of the CeNFI-DBD (top line) is aligned to a consensus sequence from the 4 mouse NFI-DBDs (bottom line). Gaps in the mouse consensus indicate residues that are not identical between the 4 mouse NFI-DBDs. The dash in the 6th aligned row indicates a single insertion in the CeNFI-DBD sequence needed to align it with the mouse consensus. Dark gray boxes show identical residues in CeNFI-DBD and mouse NFI-DBDs, light gray boxes show residues not identical but similar between CeNFI and mouse NFI-DBDs, unboxed residues with black letters show residues that are not similar between the CeNFI and mouse DBDs, and gray letters in the CeNFI-DBD above gaps in the mouse consensus indicate positions where the 4 mouse genes are not identical. 151 of 190 residues are identical in all 4 mouse NFI-DBDs while 60 of these 151 residues are different in the CeNFI-DBD and 27 of these 60 differences are non-conservative substitutions. The alignment was done in Macvector 6.5.3 using the ClustalW similarity matrix.

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DNA binding by CeNFI is indistinguishable from that of human NFI-C

The predicted DNA-binding domain of nfi-1 (CeNFI-DBD) shares homology with the vertebrate NFI proteins, however 60 of 151 residues that are completely conserved among the 4 mouse NFI proteins are changed in CeNFI (Fig. 1B). To assess the DNA-binding activity and specificity of CeNFI, the DNA-binding domain (DBD) of CeNFI was cloned in frame with a 6 histidine-tag, expressed in E. coli, partially purified by nickel-affinity chromatography, and was used for in vitro DNA binding assays. The DNA-binding activity of CeNFI-H6 was indistinguishable from that of the human hNFI-C220H6, with both proteins binding the NFI-site oligo 2.6 (Fig. 2A) but not the C2 oligo containing a point mutation that prevents vertebrate NFI binding [19, 38]. To ask if wild-type C.elegans contains a protein with similar DNA binding properties as CeNFI-H6, extracts were prepared from a mixed population of worms and tested for binding to the 2.6 and C2 sites. As expected, proteins were detected that bound to the 2.6 but not the C2 site, confirming the binding specificity of CeNFI (Fig. 2B). The specificity of the native and recombinant CeNFI proteins was also measured by competition of binding of the 2.6 oligo with various unlabeled oligonucleotides and was indistinguishable from the specificity of hNFI-C220-H6 (data not shown). Thus despite the differences between CeNFI and vertebrate NFIs in conserved residues of their DNA-binding domains, their DNA-binding activities are indistinguishable.

Figure 2
figure 2

A) Specific NFI DNA-binding activity of recombinant CeNFI-H6. Partially purified recombinant H6-tagged CeNFI (containing parts of exons 2–8) and human NFI-C220 were incubated with a duplex oligonucleotide containing an NFI binding site (2.6, even lanes) or an oligo with a single point mutation that abolishes NFI binding (C2, odd lanes) and analyzed on a 6.5% non-denaturing polyacrylamide gel. Lanes 1 and 2, crude E. coli extract (neg. control); lanes 3 and 4, ~5 ng partially purified CeNFI-H6; lanes 5 and 6, ~40 ng partially purified CeNFI-H6; lanes 7 and 8, ~5 ng purified human NFI-C220H6. See bottom of panel for sequences of oligonucleotides. B) Specific NFI DNA-binding activity in worm extracts. Nuclear extracts of a mixed population of C. elegans were prepared and used in a gel mobility shift assay with an oligonucleotide that contains an NFI-binding site (lanes 1 & 2, 2.6) or the same oligo with a single point mutation that abolishes NFI binding (lane 3, C2). Lane 1, no extract; Lanes 2 and 3, C. elegans extract (~10 μg). See A for sequences of oligonucleotides.

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Expression of nfi-1in embryo and adults

In the mouse, the 4 NFI genes are expressed in a complex overlapping pattern during embryogenesis and in adult tissues [13]. To assess the expression pattern of nfi-1 transcripts in C. elegans, a digoxin-labeled antisense probe from the 3' end of the nfi-1 transcript was used for in-situ hybridization to fixed embryos and whole worms [39]. nfi-1 transcripts are present in the one-cell (not shown) and two-cell stages (Fig. 3a) prior to the onset of zygotic transcription [40, 41], indicating that the transcript is maternally inherited. Expression continues in most cells of the embryo throughout early and mid-embryogenesis (Fig. 3b–d) but decreases after gastrulation and no expression is seen in L1 larvae (Fig. 3f–i and data not shown). As expected for a maternally inherited transcript, expression of nfi-1 reappears in the adult gonad (Fig. 3j–m). Expression of nfi-1 transcripts are also seen in the cytoplasm of gut cells (Fig. 3j–m). No signal is seen using control sense probes (Fig. 3n–o and data not shown). This in situ expression pattern indicates that nfi-1 could function both early in embryogenesis and in adult worms.

Figure 3
figure 3

Expression of endogenous nfi-1 mRNA in embryo and adults. N2 worms were fixed and hybridized with digoxigenin (DIG)-labeled antisense nfi-1 probe (from plasmid yk42f10) and bound probe was detected using alkaline phosphatase-conjugated anti-DIG antibodies. Panels: a, 2 cell embryo; b, 4 cell embryo; c, 24 cell embryo; d, beginning gastrulation; e, mid-gastrulation; f, late gastrulation; g, comma stage; h, 1.5-fold stage and i, 2-fold stage. Embryos in a-d and f-i are positive for staining. We are currently investigating the apparent loss of signal at mid-gastrulation (panel e). Panels j-m, antisense probe, adults with exposed internal organs: mature oocytes; intestine; gonadal germ cells; n-o, control sense probe, no specific staining is seen. Right panels k, m, o are two-fold magnifications of those on the left.

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Isolation of a null allele of nfi-1

To assess the role of nfi-1 in worms, we isolated a nfi-1 mutant using a reverse genetic approach based on the insertion-excision of the transposon Tc1 [42]. Worms carrying an ~2 kb deletion in nfi-1 were isolated by PCR screening and sib-selection (Fig. 4A,B). Sequence analysis of the nfi-1 transposon excision allele (designated nfi-1(qa524)) showed loss of the genomic region corresponding to nucleotides 14754–16715 of cosmid ZK1290. This eliminates the first 6 exons of nfi-1 including sequences encoding the DNA-binding domain. RT-PCR shows the absence of nfi-1 transcripts in mutant worms (Fig. 4C). A gel mobility shift assay, using nuclear extracts prepared from mix-stage populations of C. elegans wild type and nfi-1 mutant worms confirmed the loss of CeNFI DNA-binding activity in the mutant worms (Fig 4D). Thus, this mutation in the nfi-1 gene is a null allele. The survival of worms homozygous for the null allele shows that nfi-1 is not essential for worm survival. The nfi-1 mutant allele was backcrossed 12 times to the wild-type N2 strain to remove unwanted mutations prior to assessment of the phenotype.

Figure 4
figure 4

A) Deletion in C. elegans nfi-1 gene. The relative locations of confirmed exons (boxes) are shown. Square brackets indicate the location of the NFI-1 DNA-binding domain and the region deleted in nfi-1 mutant. The arrows indicate the position of the Tc1 insertion in an intron of the nfi-1 gene in strain NL747 pk240 and locations of PCR primers used in the screening for the deletion. B) Single-worm PCR reactions on N2 worm and nfi-1 homozygous mutant isolated by sib-selection. The arrows indicate a 1007 bp and 2969 bp PCR products corresponding to the nfi-1 mutant (qa524) and wild type alleles respectively. Lane 2 is 1 kb DNA ladder. C) RT-PCR on N2 worms and nfi-1 homozygous mutants. The arrows indicate a 480 bp and 320 bp RT-PCR products amplified using total RNA obtained from N2 worms (lane 1). nfi-1 mutants show loss of nfi-1 transcripts (lane 2). Lane (3) is 100 bp DNA ladder. D) Loss of NFI DNA-binding activity in extracts of nfi-1 mutants. Nuclear extracts of a mixed population of N2 worms and nfi-1 mutants were prepared and used in a gel mobility shift assay with an oligonucleotide (2.6) that contains an NFI-binding site (lanes 3, 5) or the same oligo with a single point mutation that abolishes NFI binding (C2) (lane 4, 6). See Fig. 2A for sequences of oligonucleotides. Extract of nfi-1 mutants show loss of NFI DNA-binding activity (lanes 5, 6). Lanes 1 & 2, no extract.

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Phenotype of nfi-1mutant worms

Locomotion defect

Loss of nfi-1 results in a body movement defect (Unc, uncoordinated). nfi-1 mutant animals are fairly active and healthy, but are sluggish and flaccid at rest and slightly longer and thinner than N2 worms (Fig. 5A,D). While wild type worms usually move in straight long lines, nfi-1 mutant worms often change direction abruptly (Fig. 5B,E). The nfi-1mutant worms produce less regular tracks on the bacterial lawn with higher amplitude, and sometimes are slightly coiled when compared to wild-type worms (Fig. 5C,F). This phenotype is more severe in older adults.

Figure 5
figure 5

Locomotion in nfi-1 mutants. Single young adults were spotted in the center of fresh plates and left for 10 min. (A, D) Photographs of N2 worms and nfi-1 mutants; (B, E) Track patterns of N2 worms and nfi-1 mutants; (C, F) Track patterns of N2 worms and nfi-1 mutants with higher magnification. Note less regular tracks in nfi-1 mutant vs. N2 worms.

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Egg-laying defect (Egl)

17–35% of older nfi-1 mutants have a "bag of worms" phenotype, where the mother is unable to lay fertilized eggs and fills with hatched progeny (Fig. 6A). Appearance and severity of this egg-laying phenotype correlates with the progressive locomotion defect, as young adult nfi-1 mutant worms do not bag. In young adults, serotonin stimulated egg-laying in both the wild type and nfi-1 mutant animals to a similar extent (data not shown), indicating that the postsynaptic response to serotonin is normal and the contractile apparatus for egg-laying is intact in nfi-1 mutant worms.

Figure 6
figure 6

A) Egg-laying defect in nfi-1 mutants and transgenic rescue. Bagging was measured in wild-type N2, nfi-1, N2 worms carrying the transgenic array qaEx507(N2 qaEx507) and nfi-1 worms carrying this array (nfi-1 qaEx507). Bars represent % of bagging as the mean of 3–4 independent experiments and error bars show the standard deviation. 30–75 worms of each genotype were scored in each independent experiment. N2 and N2 qaEx507showed <5% bagging. The nfi-1 mutant worms showed ~30% bagging while the rescued nfi-1 qaEx507showed <5% bagging. B) Shortened life span in nfi-1 mutants and transgenic rescue. Survival curves for the strains described above N2 (n = 57), nfi-1 (n = 58), N2 qaEx507 (n = 52) and nfi-1 qaEx507 (n = 31) are shown. Kaplan-Meier analysis (SPSS11 software) was use to determine median, percentile and p values (log rank test) and Excel was used to construct survival curves. The array generated ~50% rescue of the life-span. The experiment was repeated twice with similar results.

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Life-span reduction

The nfi-1 mutant worms have a median life span of 10.00 ± 0.69 days as compared to 14.00 ± 0.76 days for wild type worms (p < 0.001) (Fig. 6B). Mutant worms become progressively more sluggish and flaccid as they age. We are currently investigating whether this lifespan reduction is due to the apparent progressive muscle weakness and partial paralysis seen or to a direct effect on known ageing pathways [43–45].

Pharyngeal pumping rate defect

Since the Unc and Egl phenotypes of nfi-1 mutant worms could reflect aberrant muscle function, we examined another process that can influenced by muscle defects, pharyngeal pumping rate. Pharyngeal pumping rates are reduced in nfi-1 mutant worms (Table 1, Days 1–4), with more severe reductions in older vs. younger adult animals (Table 1, Days 3&4 vs. Day 1).

Table 1 Rescue of pharyngeal pumping defect by nfi-1 transgene
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Rescue of nfi-1 mutant with nfi-1transgene

Transgenic rescue was performed to test whether loss of the nfi-1 gene was responsible for the observed phenotypes. Transgenic strain XA512 qaEx507 was made by injecting a plasmid containing a 10 kb region of genomic DNA including the nfi-1 coding region and ~4 kb of upstream promoter region together with a rol-6(gf) expressing plasmid into the gonads of N2 worms. We crossed the resulting transgenic array into nfi-1 mutant worms to produce strain XA550 nfi-1(qa524) qaEx507. Egg-laying was completely rescued in nfi-1 qaEx507 worms when compared to the nfi-1 mutant worms (Fig. 6A). In addition, the median life span in nfi-1 qaEx507 worms of 13.0 ± 0.7 days was significantly longer than the 10.0 ± 0.7 day life span of nfi-1 mutant worms (p < 0.05), but slightly less than the 14.0 ± 0.8 day life span of N2 worms (Fig. 6B). The N2 qaEx507 stain used as a control has a 14.00 ± 0.49 day median life span, identical to that in non-transgenic N2 worms. The pharyngeal pumping defect was also partially rescued by transgenic expression of nfi-1 (Table 1, nfi-1 qaEx507 vs. nfi-1). The nfi-1 transgene had little or no effect on pumping rates in N2 worms (N2 qaEx507 vs. N2). These data provide a well-defined developmental system affected by loss of nfi-1 that can be examined for cell-autonomous or inductive roles of nfi-1. The presence of the rol-6 marker gene prevented scoring of rescue of the locomotion phenotype in nfi-1 qaEx507 worms.

Our in situ hybridization data indicate that nfi-1 transcripts are provided maternally. To test whether maternal nfi-1 transcripts could rescue the nfi-1 Egl phenotype, nfi-1 qa524/qa524, nfi-1 qa524/+ and +/+ progeny of heterozygous nfi-1 qa524/+ parents were scored for the egg-laying defect (Fig. 7). Bagging was seen in 41% of the resulting nfi-1 qa524/ qa524 worms, in 20.7% of nfi-1 qa524/+ worms but in <3% of +/+ animals. These data indicate the absence of maternal transcript rescue and possible haploinsufficiency at the nfi-1 locus. Thus, transgenic replacement of nfi-1 yields either partial (pumping rate and lifespan) or complete (egg-laying) rescue of the phenotypes seen in the nfi-1 mutant worms whereas maternal nfi-1 transcripts are insufficient to rescue the egg-laying defect.

Figure 7
figure 7

Haploinsufficiency of nfi-1 locus. Egg-laying defect in progeny of nfi-1 heterozygous mutant animals are shown. Bagging was scored in all progeny of two nfi-1(qa524/+) heterozygous worms (n = 146) derived from eggs laid over 7 hours. All worms were genotyped by single-worm PCR. Bars represent % of bagging in wild type (+/+), nfi-1 heterozygous (qa524/+) and nfi-1 homozygous worms (qa524/qa524). The number of worms scored of each genotype are shown above the bars.

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Ce titin expression is reduced in nfi-1mutant worms

We used cDNA microarrays to identify genes whose expression is affected by loss of nfi-1. Such genes could be either direct or indirect targets of nfi-1. Poly A+ RNA was purified from wild type and nfi-1 mutant synchronized gravid adults, labeled, and used to probe DNA microarrays containing ~17,000 C. elegans genes (Stanford Microarray Database). We analyzed RNA from gravid adults because the phenotype differences between nfi-1 mutant and wild type animals are clearer in adults than at earlier stages. The nfi-1 gene was scored as the most down-regulated gene in nfi-1 mutant worms in all experiments (data not shown). Several dozen genes showed small apparent reductions or increases in levels (2–3 fold) in mutant adults (data not shown) but only one gene, C. elegans titin (Ce titin) showed larger changes.

Ce titin (also known as tag-58, temporarily assigned gene 58) was predicted to be 5.7-fold lower in mutant worms by microarray analysis. Quantitative PCR confirmed that Ce titin is reduced 8–11 fold in adult nfi-1mutant worms (Fig. 8). To date this is the gene that shows the largest decrease in expression in nfi-1 mutant worms. A search of the Ce titin gene reveals no overabundance of NFI binding sites (data not shown). Also, a CeTPro transgene expressing a translational fusion of GFP to the 5'-end of the Ce titin gene [46] appears to be expressed at similar levels in WT and nfi-1 mutant worms (data not shown). It will be important in future studies to determine whether Ce titin is a direct or indirect target of nfi-1 and the possible role of Ce titin in the phenotypes observed.

Figure 8
figure 8

Down regulation of Ce titin expression in nfi-1 mutants assessed by QPCR. Bars represent fold changes in Ce titin transcript level in wild type N2 vs. nfi-1 mutant worms. RNA samples were obtained from 3 independent synchronized adult worm populations for each genotype.

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Expression of nfi-1::GFPreporter transgenes

One limitation of in situ hybridization in C. elegans is that in older embryos and postembryonic stages it is sometimes not sensitive enough to unambiguously identify individual cells. Since we saw little or no nfi-1 expression in muscle by in situ hybridization, in an effort to develop a more sensitive assay for nfi-1 expression we constructed two GFP-reporter transgenes (Fig. 9A). In Pro1CeNFI::GFP, 4 kb of genomic DNA sequence upstream of the nfi-1 open reading frame and the sequence encoding the first four residues of the CeNFI protein was fused in frame to GFP. In Pro2CeNFI::GFP, the 4 kb promoter region and the sequence encoding the first 94 residues of CeNFI has been fused to GFP. GFP expression for both transgenes was detected in embryos (Fig. 9B). Faint GFP expression is first detected at the late gastrulation stage of embryogenesis (>300 cells) as a diffuse green glow throughout the embryo. By the comma stage expression is detected in many cells along the outer edge of the embryo and expression continues through embryogenesis and is detected in L1-L4 larvae in many of the same cells as in adults. Adult transgenic animals show GFP expression in muscles, neurons and intestinal cells (Fig. 9B–I). Among the muscles, fluorescence was strongest in the pharynx and head muscles, was observed with less frequency in other body wall muscles and was seen occasionally in vulva muscles. Expression was also seen in two pairs of neurons located near the posterior bulb of the pharynx, and in several as yet unidentified tail neurons. Expression patterns in multiple transgenic lines from each reporter were similar with the exception that Pro2CeNFI::GFP expression was detected more consistently in head neurons and Pro1CeNFI::GFP was seen with higher frequency in body-wall muscles. However expression of both transgenes was mosaic, showing expression in only subsets of cells and animals in each population. Since mosaic expression of GFP was seen in transgenic strains from both arrays, transgenic array Pro1CeNFI::GFP was integrated by γ-irradiation. However similar mosaic expression was seen with the integrated array (data not shown). These data may indicate that additional elements are needed for stable regulation of nfi-1 expression and that such elements may be located further downstream in the nfi-1 genomic sequence.

Figure 9
figure 9

Expression pattern of the nfi-1::GFP reporter transgenes. The nfi-1 locus and structure of the nfi-1-GFP fusion constructs are shown (A). Nfi-1 coding regions are shown in black, gray boxes indicate alternatively spliced exons, untranslated regions are in white. GFP is shown as a hatched box. Expression of nfi-1-GFP reporter constructs was observed in embryos (B), intestinal cells (C), body wall muscles (D, E), pharynx (F), egg-laying muscles (G), several head (H) and tail neurons (I). Expression was assessed using a FXA Nikon microscope (B-D, F-I) and a Bio-Rad confocal microscope (E).

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Discussion

nfi-1gene structure and DNA binding properties

These data show that nfi-1 is not essential for embryogenesis and thus is not essential for DNA replication. However the multiple defects seen in nfi-1-deficient worms, abnormal locomotion, pharyngeal pumping and egg-laying defects, and a reduced lifespan indicate that nfi-1 is essential for these developmental and behavioral processes. nfi-1 shares a number of properties with the vertebrate NFI genes, including alternative splicing (Fig. 1) and the DNA-binding properties of its protein product CeNFI (Fig. 2B,C). The alternative splicing seen in nfi-1 is reminiscent of the complex splicing pattern seen in the vertebrate NFI genes [22]. While the relevance of this alternative splicing in C. elegans is unclear, the finding that alternatively spliced forms of vertebrate NFIs have different transcriptional modulation properties [16, 47, 48] suggests that the multiple C. elegans isoforms may also have distinct functions. It will be of particular interest to determine whether alternatively spliced isoforms of nfi-1 are differentially expressed in worm cells during development and in adults. In addition, when specific downstream targets of nfi-1 are identified it will be important to determine whether the isoforms differ in their ability to modulate gene expression in C. elegans.

The sequence conservation of the predicted DNA-binding domain of nfi-1 with the vertebrate NFI proteins was the initial indication that nfi-1 encodes the single C. elegans NFI gene. However among the 4 mouse (and human) NFI genes, 151 of 190 residues of their DNA-binding domains are completely conserved, while 60 of these 151 residues are changed in CeNFI (Fig. 1B). We show that CeNFI binds to the same DNA sequence as hNFI-C and that binding is abolished by a point mutation known to abolish binding by products of the 4 vertebrate NFI genes (Fig. 2) [38]. This raises the question of why there has been so little divergence of the 4 vertebrate NFI DNA-binding domains during evolution. While we have shown similar DNA binding specificities of CeNFI and hNFI-C to a matched set of WT and mutant NFI binding sites, it is possible that subtle differences exist in their DNA-binding specificity or affinity or that the conserved vertebrate residues are more important for binding within the more complex vertebrate genome. Since the 4 vertebrate NFI genes are sometimes expressed in the same cells in vivo [13], it is possible that there has been selection for conservation of residues involved in DNA binding due to competition between the 4 NFI gene products, and that this has led to the observed high degree of sequence conservation. Similar high levels of sequence conservation are seen in the DNA-binding domains of all the homologous vertebrate NFI genes from Xenopus [49, 50] to humans [12, 51].

It is possible that the additional conserved residues in the vertebrate NFI DBDs compared to nfi-1 confer additional functions to the vertebrate NFI proteins, such as interacting with proteins required for transcriptional modulation specifically in vertebrates, or for the stimulation of Ad DNA replication. Indeed, 2 sets of mutations in the NFI-C DBD that abolish Ad replication without affecting DNA-binding or dimerization are in residues not conserved between NFI-C and CeNFI [52]. However, each of the 4 cysteine residues shown previously to be essential for DNA-binding activity and redox-regulation of DNA-binding activity of vertebrate NFIs are conserved in CeNFI (Fig. 1B, labeled C) [53, 54]. The conservation of these cysteine residues is consistent with our observation that the DNA-binding activity of CeNFI is sensitive to reversible inactivation by chemical oxidizing agents such as diamide in vitro (data not shown), as is the activity of the vertebrate NFIs [53, 54]. Given the divergence of the CeNFI and vertebrate NFI DBDs it will be important to determine whether the nfi-1 DBD can substitute for the vertebrate NFI DBDs in vivo, and whether the vertebrate NFI DBDs possess equivalent activities in C. elegans in vivo.

Defects in nfi-1mutant worms

Deletion of the nfi-1 gene results in a number of behavioral defects, each of which may be related to defects in muscle or neural function. The C. elegans hermaphrodite has four major muscles types, body wall, pharyngeal, vulval and enteric [55]. Body-wall muscles and pharyngeal muscles, used in locomotion and pumping food, respectively, function in the processes affected in nfi-1 mutants. The structures most often affected in Unc mutants are the body-wall muscles, the nervous system and the hypodermis [56]. Polarized light microscopy showed no obvious deformities in the body wall-muscles of nfi-1 mutant animals, such as defects in muscle attachment and overall sarcomere structure. Thus, the muscles appear normal at a gross level. Likewise, we analyzed the expression pattern of the body wall muscle specific hlh-1::GFP [57] and muscle specific CeTPro transgenes [46] in the nfi-1 deletion mutant background by confocal microscopy and saw no differences between mutant and wild type animals (data not shown). Thus any muscle defects are not due to gross structural changes within the muscle. Many older nfi-1 deficient animals display egg-laying defects, suggesting that vulval or/and uterine muscles or their controlling neurons may be affected. The normal stimulation of egg-laying by serotonin suggests that there is no major loss of vulval or uterine muscle function in young adult nfi-1 deficient worms [58, 59]. We have visually examined the functions of the enteric muscles involved in food movement through the gut and defecation and found no obvious defects in young adults. In older adults, contractions of the enteric muscles were less regular, but it is unclear whether this is an intrinsic defect or related to the defects in pharyngeal pumping seen in older adults.

The absence of obvious muscle defects in nfi-1 null worms raises the possibility that neural defects could underlie the phenotypes seen in these animals. For example, nfi-1 mutants show a reduced pharyngeal pumping rate, a process regulated primarily by the M3, M4 and MC pharyngeal neurons [60]. Some or all of defects seen in nfi-1 mutant worms, mild Unc with flaccidity, pharyngeal pumping and egg-laying are also seen in worms containing single mutations in genes expressed in neurons. For example, some mutations in egl-30, a heterotrimeric Gqα subunit expressed in muscles and neurons, have weak Unc, egg-laying and pharyngeal defects [61]. In addition, some loss of function mutations in the G protein signaling molecule egl-10 and the Gβ5 ortholog eat-11 cause sluggish movement, egg-laying, and pharyngeal pumping defects [62, 63]. Since a number of eat genes are expressed specifically in neurons, these data indicate that neural dysfunction caused by loss of nfi-1 could generate this range of phenotypes. It will be important in future studies to determine whether nfi-1 expression is needed neurons, muscles, both cell types or other cell types to alleviate the phenotypes seen in nfi-1 mutant worms. While our preliminary QPCR data indicate no changes in the transcript levels of egl-30, egl-10 or eat-11 (N. Butz, unpublished data), it is possible that the activity of the G protein signaling system is affected in nfi-1 mutant worms. Thus, it would be useful in future studies to directly test whether nfi-1 is involved in G protein signaling in C. elegans.

The only gene whose expression has been shown to change in response to loss of nfi-1 is the muscle-specific gene Ce titin. This reduction in a muscle-specific gene indicates that muscle defects could contribute to the observed locomotion and other phenotypes. Ce titin is a massive protein, with isoforms in C. elegans of 2.2MDa, 1.2MDa and 301KDa [46]. Ce titin is found in the I-bands of larval and adult muscle in worms. Mutations in mouse titin genes cause muscular dystrophy [64], cardiac development defects and muscle weakness [65]. Thus, down regulation of Ce titin expression could contribute to the motility and egg-laying defects observed in nfi-1 mutants. However, the large size of the Ce titin gene has made it difficult to generate transgenic lines that conditionally express Ce titin. Recent studies have reported that neither existing mutations in Ce titin, nor RNAi experiments, have provided clues as to the function of Ce titin in worms [46]. Thus it will be important in the future to test directly the role of Ce titin in the defects seen in nfi-1 mutant worms.

Finally, it is unknown whether the observed motility, egg-laying and pharyngeal pumping defects contribute to the shortened life-span of nfi-1 null worms. For example, as pharyngeal pumping becomes more defective the animals could starve, leading to progressive muscle wasting, paralysis and death. However, most severe eat mutants have extended lifespans rather than reduced lifespans, most likely due to caloric restriction [66]. These data indicate that the reduced lifespan seen in nfi-1 deficient worms could be independent from the pharyngeal pumping defect. In addition, while bagging could reduce apparent lifespan, we eliminated bagged animals from our lifespan analysis so as to exclude bagging as a direct cause of the shortened lifespan. Lastly, the motility defect is seen in young adults a few days prior to bagging and is seen in all adults, even those that do not bag. Thus it appears unlikely that bagging contributes directly to the movement defect. It will be important to use directed expression of nfi-1 in different cell types to test the dependence or independence of the observed phenotypes from each other. We are also currently testing whether nfi-1 is part of the genetic pathways known to be important in worm aging including the daf pathway [67].

Roles of NFI transcription factors in development

Since NFI genes have been found in all metazoa sequenced to date and multiple NFI genes are present in complex animals [68], it was our initial hypothesis that nfi-1 would be essential in worms and that deletion of the gene would be lethal. Lethality would be predicted if nfi-1 has an essential role in DNA replication. However, all of the defects observed are late physiological defects and are most severe in older adults. This set of late defects can be compared to the late gestational defects seen with deletion of single NFI genes in mice. Disruption of the mouse Nfia gene causes agenesis of the corpus callosum, the loss of specific midline glial populations and perinatal lethality [32, 33]. Loss of Nfic produces specific defects in tooth development including aberrant incisor formation and failure of root formation in molar teeth [34]. Targeted insertion into the Nfib locus causes perinatal lethality due to a failure of late fetal lung maturation [35, 36]. One common feature of the defects seen in NFI-deficient mice is that they occur either late in fetal development (Nfia and Nfib), or early in postnatal development (Nfic). While some of the developmental systems affected by the loss of NFI genes in mice are not present in worms (e.g. lungs and teeth), it is possible that the underlying molecular mechanisms disrupted in NFI-deficient mice are also affected by loss of nfi-1 in worms. Thus it will be important to test genes identified as important in the phenotypes of NFI-deficient mice for potential roles in the motility, egg-laying and pharyngeal-pumping defects seen in nfi-1mutant worms.

Conclusion

These data show that nfi-1 is not essential for worm survival but plays important roles in locomotion, egg-laying, pharyngeal pumping and maintenance of a normal life-span. These are the first data to show that while NFI proteins are essential for adenovirus DNA replication, they are not essential for DNA replication in simple animals.

We show that while many residues of the DNA binding domain of worm NFI differ from those found in vertebrate NFIs, the DNA binding activity and specificity of the worm protein is indistinguishable from that of the vertebrate NFIs. These data suggest that the very strong conservation of many residues in the DNA-binding domains of vertebrate NFIs is not needed for DNA-binding specificity but may serve another function, perhaps for interactions with specific vertebrate proteins. In addition, like the vertebrate NFI genes nfi-1 is alternatively spliced, raising the possibility that different CeNFI isoforms may have different biological functions.

The phenotypes of nfi-1 null worms are rescued by zygotic expression of nfi-1 from its natural promoter but not by maternal transcripts. We propose that nfi-1 regulates gene expression directly in cells in which it is expressed and affect the function of these cells. However, the precise cell types in which loss of nfi-1 causes these defects in motility, egg-laying, pharyngeal pumping and lifespan is unknown. Directed expression of nfi-1 in different cells of the worm should allow us to determine in which cells nfi-1 is needed for normal lifespan, motility, egg-laying and pharyngeal pumping.

Future studies will assess whether C. elegans can be used as an experimental system to assess the potentially distinct physiological functions of the vertebrate NFI gene products. For example, if the 4 vertebrate NFI genes have segregated the functions of the single worm nfi-1 gene into 4 distinct entities, then each vertebrate NFI gene might have distinct properties when expressed in nfi-1mutant worms. It will also be important to determine the specific nfi-1 target genes in C. elegans whose altered expression is responsible for the phenotypes observed, the specific cell types in which nfi-1 must be expressed to prevent each phenotype, and to use C. elegans genetics to identify genes essential for NFI-regulated transcription.

Methods

Strains and transgene plasmids

The Bristol strain N2 was used as wild type and worms were grown at 20°C using standard techniques [69]. Strains containing hlh-1:GFP, CeTPro and NL747 pk240 were kindly provided by Michael Krause (NIH/NIDDK/LMB, Bethesda, MD), Guy M. Benian (Emory University School of Medicine, Atlanta, GA) and Ronald Plasterk (Hubrecht Laboratory, Utrecht, The Netherlands), respectively.

For rescue experiments the nfi-1 locus from cosmid ZK1290 was cloned into pBluescriptKS+ to generate pCeNFIG. pCeNFIG (25 ng/ul) was injected in N2 wild type worms along with the rol-6(gf) marker plasmid pRF4 (125 ng/ul) using standard microinjection procedures to produce the transgenic strain XA512 qaEx507 [69]. Transgenic arrays were crossed onto the nfi-1 deletion strain (XA549 nfi-1(qa524), see below) screening F1 and F2 progeny by PCR for the wild-type and nfi-1(qa524) deletion alleles.

Two GFP-reporter constructs were made to assess nfi-1 expression. Pro1CeNFI::GFP was made by cloning a PCR-generated HindIII-XmaI fragment of ZK1290 into pPD95.69 (kindly provided by A. Fire) to generate a translational fusion of nfi-1 and GFP. The 5'end of the construct was extended by cloning the HindIII-BglII fragment of pCeNFIG into the vector. Pro2CeNFI::GFP was made by cloning a HindIII-PstI fragment of pCeNFIG into corresponding sites of pPD95.69. The constructs were coinjected with rol-6 marker into N2 worms as described above. Three independent transgenic lines were analyzed for Pro1CeNFI::GFP expression and two lines for Pro2CeNFI::GFP expression.

A vector expressing the 6his-tagged DNA-binding domain of CeNFI (pCeNFI-H6) in E. coli was produced by cloning a fragment of nfi-1 cDNA encompassing the predicted DNA-binding domain of nfi-1 downstream of a 6 histidine tag into the pET8C vector [70].

Generation of the nfi-1deletion mutant

C. elegans NL747 pk240 contains a Tc1 transposon insertion in the 6 th intron of nfi-1. The location of the Tc1 element was mapped by genomic PCR and sequencing. 100 populations of worms were established at 15°C on 3 cm NGM/OP-50 plates starting with 10–20 worms each, and the worms were collected in M9 buffer when nearing starvation. One third of the worms were put onto fresh plates and the rest were used to prepare two separate lysates for DNA. Thirteen of the 100 populations showed a strong excision bands by PCR screening (Platinum Taq Polymerase, Invitrogen) with primers 1 (5'GTATTTGTACGACCCTCTGCG) and 2 (5'TGCTGTTGAACGGAATGCACC). To distinguish between germ line and somatic mutations the second lysate for each positive population was screened by PCR. Only two populations showed deletions with both lysates indicating that more than one worm carried the deletion and therefore the deletions were in the germ line. The locations of the deletions were determined by subcloning PCR products into pCRII-TOPO (Invitrogen) and sequencing. A clonal strain of worms carrying one of these deletions was isolated by multiple rounds of PCR screening and sib-selection (strain XA549 nfi-1(qa524)). The mutant worms were backcrossed 12 times onto the wild-type N2 strain to remove unwanted mutations. For genotyping, PCR with primers 1 and 3 (5'TCGGAGGAGGTGGTAGACAT) amplified a 623 bp product from the deletion allele and primers 1 and 4 (5'GTGAGTCTTGAGGTGCTTCTG) amplified a 968 bp product from the wild type allele.

RT-PCR cloning and cDNA isolation

Total RNA was prepared from adult worms or eggs (Trizol, Life Technologies) and was reverse transcribed using oligo dT (Superscript, Life Technologies) according to the manufacturers instructions. Poly A+ mRNA was isolated from total RNA using standard techniques (Oligotex, Qiagen). PCR was performed as described previously [14] using primers from within predicted exons 1–14 of nfi-1 or SL1 primers (GGTTTAATTACCCAAGTTTGAG) (nfi-1 primer sequences available upon request) and PCR products were cloned into the pCRII-TOPO vector (Invitrogen). DNA from individual clones was isolated (Wizard, Promega) and sequenced (Roswell Park Cancer Institute DNA Sequencing Core) and the sequence obtained compared with that of the predicted exons of nfi-1 (C. elegans cosmid ZK1290, Genbank Acc. #U21308). The cDNA clones yk42f10 and yk213C10 were from the C. elegans cDNA sequencing/expression project (CREST and Gene Network Lab, National Institute of Genetics, Mishima) and CEESQ09 was from The Institute for Genomic Research (TIGR).

In situhybridization

Endogenous nfi-1 transcripts were analyzed as described previously [39], using digoxin-labeled antisense probe for nfi-1. Embryos were either freeze-fractured or were treated with chitinase to digest the eggshell prior to hybridization.

DNA-binding assays

Electrophoretic mobility shift assays for the analysis of NFI protein binding to oligonucleotides was performed as described previously [38, 54]. Worm extracts were prepared from dounce homogenized mixed-age worms using the NP40-based extraction buffer described previously [38]. CeNFI-H6 (332aa) and hNFI-C220-H6 (230aa) proteins were produced and partially purified from extracts of E. coli as described previously [54]. The labeled oligonucleotides used contained a wild-type NFI binding site (2.6) or a site with a single point mutation (C2) shown previously to abolish the binding of vertebrate NFI proteins [19, 38, 54, 71].

Behavioral and functional assays

For locomotion assays wild type N2 and nfi-1 mutants were raised at 20°C on NGM/OP50 plates. To obtain age-synchronized worms young adults were placed on a bacterial lawn to allow them to lay eggs for 3 hours and then removed. Worms were observed at all larval stages. To photograph worms tracks, single young adult worms were moved to new plates containing a 1 day old bacterial lawn and were left undisturbed for 10 min.

"Bag of worms" phenotype was scored for each strain using age-synchronized worms. Worms were observed each day from the start of egg laying until one day after cessation of egg laying. They were moved to new plates every second day to prevent overcrowding and starvation. Other aspects of egg-laying behavior such as the brood size, stage of newly laid eggs, and response to serotonin were assessed as described previously [58, 59]. Brood size was the same in WT and nfi-1 null worms.

For life-span assays wild type N2 and nfi-1 mutants were raised and synchronized as described above except that 250 μg/ml of fungizone was included to reduce fungal contamination. Worms were maintained at 20°C and adult worms were moved to new plates every second day until progeny production ceased. Worms were observed every day and were scored as dead when they failed to respond to touch. Animals that crawled off the plate or died from internally hatched progeny were censored, but incorporated into the data until the day of disqualification.

Pharyngeal pumping was counted for one minute starting on the first day of adulthood for four consecutive days [60]. Worms were placed on NGM/OP50 plates and left undisturbed for 1 hour before measuring. All animals remained on food during the period of observation.

Microarray assays and QPCR

Synchronized populations were generated by hatching eggs after alkaline hypochlorite-treatment and the worms were collected as gravid adults. Total RNA was prepared with Trizol (Life Techologies) and poly A RNA was purified using Poly(A)Puristâ„¢ kit (Ambion). DNA microarray assays were performed and analyzed in the Stanford Microarray Database (Stanford). cDNA for QPCR was made using random primers and Super-Script (Invitrogen). QPCR was performed using AmpliTaq GoldR with the Gene AmpR SYBR green kit (Applied Biosystems) on a Bio-Rad iCycler. The inf-1 gene was used as an internal control for RT-PCR reactions and for normalized quantification in QPCR reactions. Primers used in QPCR are available on request.

References

  1. Nagata K, Guggenheimer RA, Hurwitz J: Specific binding of a cellular DNA replication protein to the origin of replication of adenovirus DNA. Proc Natl Acad Sci USA. 1983, 80: 6177-6181.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  2. Nagata K, Guggenheimer RA, Hurwitz J: Adenovirus DNA replication in vitro: Synthesis of full-length DNA with purified proteins. Proc Natl Acad Sci USA. 1983, 80: 4266-4270.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  3. Hay RT: Origin of adenovirus DNA replication: Role of the nuclear factor I binding site in vivo. J Mol Biol. 1985, 186: 129-136. 10.1016/0022-2836(85)90263-3.

    Article  CAS  PubMed  Google Scholar 

  4. Leegwater PAJ, van Driel W, van der Vliet PC: Recognition site of nuclear factor I, a sequence-specific DNA-binding protein from HeLa cells that stimulates adenovirus DNA replication. EMBO J. 1985, 4: 1515-1521.

    PubMed Central  CAS  PubMed  Google Scholar 

  5. de Jong RN, van der Vliet PC: Mechanism of DNA replication in eukaryotic cells: cellular host factors stimulating adenovirus DNA replication. Gene. 1999, 236: 1-12. 10.1016/S0378-1119(99)00249-8.

    Article  CAS  PubMed  Google Scholar 

  6. Santoro C, Mermod N, Andrews PC, Tjian R: A family of human CCAAT-box-binding proteins active in transcription and DNA replication: cloning and expression of multiple cDNAs. Nature. 1988, 334: 218-224. 10.1038/334218a0.

    Article  CAS  PubMed  Google Scholar 

  7. Guo ZS, DePamphilis ML: Specific transcription factors stimulate simian virus 40 and polyomavirus origins of DNA replication. Mol Cell Biol. 1992, 12: 2514-2524.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  8. Gronostajski RM, Knox J, Berry D, Miyamoto NG: Stimulation of transcription in vitro by binding sites for nuclear factor I. Nucl Acids Res. 1988, 16: 2087-2098.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  9. Cooke DW, Lane MD: The transcription factor nuclear factor I mediates repression of the GLUT4 promoter by insulin. J Biol Chem. 1999, 274: 12917-12924. 10.1074/jbc.274.18.12917.

    Article  CAS  PubMed  Google Scholar 

  10. Gil G, Smith JR, Goldstein JL, Slaughter CA, Orth K, Brown MS, Osborne TF: Multiple genes encode nuclear factor 1-like proteins that bind to the promoter for 3-hydroxy-3-methylglutaryl-coenzyme A reductase. Proc Natl Acad Sci USA. 1988, 85: 8963-8967.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  11. Rupp RAW, Kruse U, Multhaup G, Gobel U, Beyreuther K, Sippel AE: Chicken NFI/TGGCA proteins are encoded by at least three independent genes: NFI-A, NFI-B and NFI-C with homologues in mammalian genomes. Nucl Acids Res. 1990, 18: 2607-2616.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  12. Qian F, Kruse U, Lichter P, Sippel AE: Chromosomal localization of the four genes NFIA, B, C, and X for the human transcription factor Nuclear Factor I by FISH. Genomics. 1995, 28: 66-73. 10.1006/geno.1995.1107.

    Article  CAS  PubMed  Google Scholar 

  13. Chaudhry AZ, Lyons CE, Gronostajski RM: Expression patterns of the four Nuclear Factor I genes during mouse embryogenesis indicate a potential role in development. Dev Dyn. 1997, 208: 313-325. 10.1002/(SICI)1097-0177(199703)208:3<313::AID-AJA3>3.0.CO;2-L.

    Article  CAS  PubMed  Google Scholar 

  14. Fletcher CF, Jenkins NA, Copeland NG, Chaudhry AZ, Gronostajski RM: Exon structure of the nuclear factor I DNA-binding domain from C. elegans to mammals. Mamm Genome. 1999, 10: 390-396. 10.1007/s003359901008.

    Article  CAS  PubMed  Google Scholar 

  15. Gronostajski RM: Role of NFI/CTF gene family in transcription and development. Gene. 2000, 249: 31-45. 10.1016/S0378-1119(00)00140-2.

    Article  CAS  PubMed  Google Scholar 

  16. Mermod N, O'Neill EA, Kelly TJ, Tjian R: The proline-rich transcriptional activator of CTF/NF-1 is distinct from the replication and DNA binding domain. Cell. 1989, 58: 741-753. 10.1016/0092-8674(89)90108-6.

    Article  CAS  PubMed  Google Scholar 

  17. Kruse U, Sippel AE: Transcription factor nuclear factor I proteins form stable homo- and heterodimers. FEBS Lett. 1994, 348: 46-50. 10.1016/0014-5793(94)00585-0.

    Article  CAS  PubMed  Google Scholar 

  18. Gronostajski RM, Adhya S, Nagata K, Guggenheimer RA, Hurwitz J: Site-specific DNA binding of nuclear factor I: Analyses of cellular binding sites. Mol Cell Biol. 1985, 5: 964-971.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  19. Gronostajski RM: Analysis of nuclear factor I binding to DNA using degenerate oligonucleotides. Nucl Acids Res. 1986, 14: 9117-9132.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  20. Meisterernst M, Gander I, Rogge L, Winnacker EL: A quantitative analysis of nuclear factor I/DNA interactions. Nucl Acids Res. 1988, 16: 4419-4435.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  21. Roulet E, Bucher P, Schneider R, Wingender E, Dusserre Y, Werner T, Mermod N: Experimental analysis and computer prediction of CTF/NFI transcription factor DNA binding sites. J Mol Biol. 2000, 297: 833-848. 10.1006/jmbi.2000.3614.

    Article  CAS  PubMed  Google Scholar 

  22. Kruse U, Sippel AE: The genes for transcription factor nuclear factor I give rise to corresponding splice variants between vertebrate species. J Mol Biol. 1994, 238: 860-865. 10.1006/jmbi.1994.1343.

    Article  CAS  PubMed  Google Scholar 

  23. Bedford FK, Julius D, Ingraham HA: Neuronal expression of the 5HT3 serotonin receptor gene requires nuclear factor 1 complexes. J Neurosci. 1998, 18: 6186-6194.

    CAS  PubMed  Google Scholar 

  24. Baumeister H, Gronostajski RM, Lyons GE, Margolis FL: Identification of NFI-binding sites and cloning of NFI-cDNAs suggest a regulatory role for NFI transcription factors in olfactory neuron gene expression. Mol Brain Res. 1999, 72: 65-79. 10.1016/S0169-328X(99)00210-7.

    Article  CAS  PubMed  Google Scholar 

  25. Spitz F, Salminen M, Demignon J, Kahn A, Daegelen D, Maire P: A combination of MEF3 and NFI proteins activates transcription in a subset of fast-twitch muscles. Mol Cell Biol. 1997, 17: 656-666.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  26. Archer TK, Lefebvre P, Wolford RG, Hager GL: Transcription factor loading on the MMTV promoter - A bimodal mechanism for promoter activation. Science. 1992, 255: 1573-1576.

    Article  CAS  PubMed  Google Scholar 

  27. Spangenberg C, Eisfeld K, Stunkel W, Luger K, Flaus A, Richmond TJ, Truss M, Beato M: The mouse mammary tumour virus promoter positioned on a tetramer of histones H3 and H4 binds nuclear factor 1 and OTF1. J Mol Biol. 1998, 278: 725-739. 10.1006/jmbi.1998.1718.

    Article  CAS  PubMed  Google Scholar 

  28. Ortiz L, Aza-Blanc P, Zannini M, Cato AC, Santisteban P: The interaction between the forkhead thyroid transcription factor TTF-2 and the constitutive factor CTF/NF-1 is required for efficient hormonal regulation of the thyroperoxidase gene transcription. J Biol Chem. 1999, 274: 15213-15221. 10.1074/jbc.274.21.15213.

    Article  CAS  PubMed  Google Scholar 

  29. Cooke DW, Lane MD: Transcription Factor NF1 Mediates Repression of the GLUT4 Promoter by Cyclic-AMP. Biochem Biophys Res Commun. 1999, 260: 600-604. 10.1006/bbrc.1999.0959.

    Article  CAS  PubMed  Google Scholar 

  30. Rossi P, Karsenty G, Roberts AB, Roche NS, Sporn MB, de Crombrugghe B: A nuclear factor 1 binding site mediates the transcriptional activation of a type I collagen promoter by transforming growth factor-b. Cell. 1988, 52: 405-414. 10.1016/S0092-8674(88)80033-3.

    Article  CAS  PubMed  Google Scholar 

  31. Alevizopoulos A, Dusserre Y, Tsai-Pflugfelder M, von der Weid T, Wahli W, Mermod N: A proline-rich TGF-beta-responsive transcriptional activator interacts with histone H3. Genes Dev. 1995, 9: 3051-3066.

    Article  CAS  PubMed  Google Scholar 

  32. Shu T, Butz KG, Plachez C, Gronostajski RM, Richards LJ: Abnormal development of forebrain midline glia and commissural projections in Nfia knock-out mice. J Neurosci. 2003, 23: 203-212.

    CAS  PubMed  Google Scholar 

  33. das Neves L, Duchala CS, Godinho F, Haxhiu MA, Colmenares C, Macklin WB, Campbell CE, Butz KG, Gronostajski RM: Disruption of the murine Nuclear Factor I-A gene (Nfia) results in perinatal lethality, hydrocephalus and agenesis of the corpus callosum. Proc Natl Acad Sci USA. 1999, 96: 11946-11951. 10.1073/pnas.96.21.11946.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  34. Steele-Perkins G, Butz KG, Lyons GE, Zeichner-David M, Kim HJ, Cho MI, Gronostajski RM: Essential role for NFI-C/CTF transcription-replication factor in tooth root development. Mol Cell Biol. 2003, 23: 1075-1084. 10.1128/MCB.23.3.1075-1084.2003.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  35. Grunder A, Ebel TT, Mallo M, Schwarzkopf G, Shimizu T, Sippel AE, Schrewe H: Nuclear factor I-B (Nfib) deficient mice have severe lung hypoplasia. Mech Dev. 2002, 112: 69-77. 10.1016/S0925-4773(01)00640-2.

    Article  CAS  PubMed  Google Scholar 

  36. Steele-Perkins G, Plachez C, Butz KG, Yang GH, Bachurski CJ, Kinsman SL, Litwack ED, Richards LJ, Gronostajski RM: The transcription factor Nfib is essential for both lung maturation and brain development. Mol Cell Biol. 2005, 25: 685-698. 10.1128/MCB.25.2.685-698.2005.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  37. Blumenthal T, Steward K: RNA processing and gene structure. C elegans II. Edited by: Riddle DL, Blumenthal T, Meyer BJ and Priess JR. 1999, Cold Spring Harbor, Cold Spring Harbor Laboratory Press, 33: 117-145.

    Google Scholar 

  38. Goyal N, Knox J, Gronostajski RM: Analysis of multiple forms of nuclear factor I in human and murine cell lines. Mol Cell Biol. 1990, 10: 1041-1048.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  39. Tabara H, Motohashi T, Kohara Y: A multi-well version of in situ hybridization on whole mount embryos of Caenorhabditis elegans. Nucl Acids Res. 1996, 24: 2119-2124. 10.1093/nar/24.11.2119.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  40. Edgar LG, Wolf N, Wood WB: Early transcription of Caenorhabditis elegans embryos. Development. 1994, 120: 443-451.

    CAS  PubMed  Google Scholar 

  41. Seydoux G, Fire A: Soma-germline asymmetry in the distributions of embryonic RNAs in Caenorhabditis elegans. Development. 1994, 120: 2823-2834.

    CAS  PubMed  Google Scholar 

  42. Zwaal RR, Broeks A, van Meurs J, Groenen JT, Plasterk RH: Target-selected gene inactivation in Caenorhabditis elegans by using a frozen transposon insertion mutant bank. Proc Natl Acad Sci USA. 1993, 90: 7431-7435.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  43. Murphy CT, McCarroll SA, Bargmann CI, Fraser A, Kamath RS, Ahringer J, Li H, Kenyon C: Genes that act downstream of DAF-16 to influence the lifespan of Caenorhabditis elegans. Nature. 2003, 424: 277-283. 10.1038/nature01789.

    Article  CAS  PubMed  Google Scholar 

  44. Kimura KD, Tissenbaum HA, Liu Y, Ruvkun G: daf-2, an insulin receptor-like gene that regulates longevity and diapause in Caenorhabditis elegans. Science. 1997, 277: 942-946. 10.1126/science.277.5328.942.

    Article  CAS  PubMed  Google Scholar 

  45. Holzenberger M, Dupont J, Ducos B, Leneuve P, Geloen A, Even PC, Cervera P, Le Bouc Y: IGF-1 receptor regulates lifespan and resistance to oxidative stress in mice. Nature. 2003, 421: 182-187. 10.1038/nature01298.

    Article  CAS  PubMed  Google Scholar 

  46. Flaherty DB, Gernert KM, Shmeleva N, Tang X, Mercer KB, Borodovsky M, Benian GM: Titins in C.elegans with unusual features: coiled-coil domains, novel regulation of kinase activity and two new possible elastic regions. J Mol Biol. 2002, 323: 533-549. 10.1016/S0022-2836(02)00970-1.

    Article  CAS  PubMed  Google Scholar 

  47. Chaudhry AZ, Vitullo AD, Gronostajski RM: Nuclear factor I (NFI) isoforms differentially activate simple versus complex NFI-responsive promoters. J Biol Chem. 1998, 273: 18538-18546. 10.1074/jbc.273.29.18538.

    Article  CAS  PubMed  Google Scholar 

  48. Chaudhry AZ, Vitullo AD, Gronostajski RM: Nuclear factor I-mediated repression of the mouse mammary tumor virus promoter is abrogated by the coactivators p300/CBP and SRC-1. J Biol Chem. 1999, 274: 7072-7081. 10.1074/jbc.274.11.7072.

    Article  CAS  PubMed  Google Scholar 

  49. Roulet E, Armentero MT, Krey G, Corthesy B, Dreyer C, Mermod N, Wahli W: Regulation of the DNA-binding and transcriptional activities of Xenopus laevis NFI-X by a novel C-terminal domain. Mol Cell Biol. 1995, 15: 5552-5562.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  50. Puzianowska-Kuznicka M, Shi YB: Nuclear factor I as a potential regulator during postembryonic organ development. J Biol Chem. 1996, 271: 6273-6282. 10.1074/jbc.271.11.6273.

    Article  CAS  PubMed  Google Scholar 

  51. Kruse U, Qian F, Sippel AE: Identification of a fourth Nuclear Factor I gene in chicken by cDNA cloning: NFIX. Nucl Acids Res. 1991, 19: 6641-

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  52. Armentero MT, Horwitz M, Mermod N: Targeting of DNA polymerase to the adenovirus origin of DNA replication by interaction with nuclear factor I. Proc Natl Acad Sci USA. 1994, 91: 11537-11541.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  53. Bandyopadhyay S, Starke DW, Mieyal JJ, Gronostajski RM: Thioltransferase (Glutaredoxin) reactivates the DNA-binding activity of oxidation-inactivated Nuclear Factor I. J Biol Chem. 1998, 273: 392-397. 10.1074/jbc.273.1.392.

    Article  CAS  PubMed  Google Scholar 

  54. Bandyopadhyay S, Gronostajski RM: Identification of a conserved oxidation-sensitive cysteine residue in the NFI family of DNA-binding proteins. J Biol Chem. 1994, 269: 29949-29955.

    CAS  PubMed  Google Scholar 

  55. Moerman DG, Fire A: Muscle: Structure, Function, and Development. C elegans II. Edited by: Riddle DL, Blumenthal T, Meyer BJ and Priess JR. 1997, Cold Spring Harbor, Cold Spring Harbor Laboratory Press, 33: 417-470.

    Google Scholar 

  56. Harris TW, Chen N, Cunningham F, Tello-Ruiz M, Antoshechkin I, Bastiani C, Bieri T, Blasiar D, Bradnam K, Chan J, Chen CK, Chen WJ, Davis P, Kenny E, Kishore R, Lawson D, Lee R, Muller HM, Nakamura C, Ozersky P, Petcherski A, Rogers A, Sabo A, Schwarz EM, Van Auken K, Wang Q, Durbin R, Spieth J, Sternberg PW, Stein LD: WormBase: a multi-species resource for nematode biology and genomics. Nucleic Acids Res. 2004, 32 Database issue: D411-7. 10.1093/nar/gkh066.

    Article  Google Scholar 

  57. Krause M, Harrison SW, Xu SQ, Chen L, Fire A: Elements regulating cell- and stage-specific expression of the C. elegans MyoD family homolog hlh-1. Dev Biol. 1994, 166: 133-148. 10.1006/dbio.1994.1302.

    Article  CAS  PubMed  Google Scholar 

  58. Trent C, Tsuing N, Horvitz HR: Egg-laying defective mutants of the nematode Caenorhabditis elegans. Genetics. 1983, 104: 619-647.

    PubMed Central  CAS  PubMed  Google Scholar 

  59. Shyn SI, Kerr R, Schafer WR: Serotonin and Go modulate functional states of neurons and muscles controlling C. elegans egg-laying behavior. Curr Biol. 2003, 13: 1910-1915. 10.1016/j.cub.2003.10.025.

    Article  CAS  PubMed  Google Scholar 

  60. Avery L, Horvitz HR: Pharyngeal pumping continues after laser killing of the pharyngeal nervous system of C. elegans. Neuron. 1989, 3: 473-485. 10.1016/0896-6273(89)90206-7.

    Article  CAS  PubMed  Google Scholar 

  61. Brundage L, Avery L, Katz A, Kim UJ, Mendel JE, Sternberg PW, Simon MI: Mutations in a C. elegans Gqalpha gene disrupt movement, egg laying, and viability. Neuron. 1996, 16: 999-1009. 10.1016/S0896-6273(00)80123-3.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  62. Koelle MR, Horvitz HR: EGL-10 regulates G protein signaling in the C. elegans nervous system and shares a conserved domain with many mammalian proteins. Cell. 1996, 84: 115-125. 10.1016/S0092-8674(00)80998-8.

    Article  CAS  PubMed  Google Scholar 

  63. Robatzek M, Niacaris T, Steger K, Avery L, Thomas JH: eat-11 encodes GPB-2, a Gbeta(5) ortholog that interacts with G(o)alpha and G(q)alpha to regulate C. elegans behavior. Curr Biol. 2001, 11: 288-293. 10.1016/S0960-9822(01)00074-4.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  64. Witt CC, Ono Y, Puschmann E, McNabb M, Wu Y, Gotthardt M, Witt SH, Haak M, Labeit D, Gregorio CC, Sorimachi H, Granzier H, Labeit S: Induction and Myofibrillar Targeting of CARP, and Suppression of the Nkx2.5 Pathway in the MDM Mouse with Impaired Titin-based Signaling. J Mol Biol. 2004, 336: 145-154. 10.1016/j.jmb.2003.12.021.

    Article  CAS  PubMed  Google Scholar 

  65. Gotthardt M, Hammer RE, Hubner N, Monti J, Witt CC, McNabb M, Richardson JA, Granzier H, Labeit S, Herz J: Conditional expression of mutant M-line titins results in cardiomyopathy with altered sarcomere structure. J Biol Chem. 2003, 278: 6059-6065. 10.1074/jbc.M211723200.

    Article  CAS  PubMed  Google Scholar 

  66. Lakowski B, Hekimi S: The genetics of caloric restriction in Caenorhabditis elegans. Proc Natl Acad Sci USA. 1998, 95: 13091-13096. 10.1073/pnas.95.22.13091.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  67. Nasrin N, Ogg S, Cahill CM, Biggs W, Nui S, Dore J, Calvo D, Shi Y, Ruvkun G, Alexander-Bridges MC: DAF-16 recruits the CREB-binding protein coactivator complex to the insulin-like growth factor binding protein 1 promoter in HepG2 cells. Proc Natl Acad Sci USA. 2000, 97: 10412-10417. 10.1073/pnas.190326997.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  68. Murtagh J, Martin F, Gronostajski RM: The Nuclear Factor I (NFI) gene family in mammary gland development and function. J Mamm Gland Biol Neopl. 2003, 8: 241-254. 10.1023/A:1025909109843.

    Article  Google Scholar 

  69. Wood WB: The Nematode Caenorhabditis elegans. Edited by: Wood WB. 1988, Cold Spring Harbor, New York, Cold Spring Harbor Laboratory

    Google Scholar 

  70. Studier FW, Rosenberg AH, Dunn JJ, Dubenforff JW: Use of T7 RNA polymerase to direct expression of cloned genes. Meth Enzymol. 1990, 185: 60-89.

    Article  CAS  PubMed  Google Scholar 

  71. Gronostajski RM: Site-specific DNA binding of nuclear factor I: effect of the spacer region. Nucl Acids Res. 1987, 15: 5545-5559.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

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Acknowledgements

The authors thank Ann Billetz and Emily Harville for initial studies on the nfi-1 gene. We also thank the Plasterk lab for isolation of the Tc1 insertion in the nfi-1 gene, the Kim lab and the Stanford Microarray Database facility for cDNA array screening, Drs. Michael Krause (NIH/NIDDK/LMB, Bethesda, MD), Wayne Materi and Dave Pilgrim (Univ. of Alberta, Edmonton, Alberta, CA), Jim McGhee (Univ. of Calgary, Calgary, Alberta, CA) and the C. elegans Genetic Center (Univ. of Minnesota, St. Paul, MN) for transgenic strains, the C. elegans Genomic Sequencing Consortium (Sanger Center, Cambridge UK for cosmid ZK1290 and TIGR for cDNA clones. Drs. Phil Morgan and Marge Sedensky (Case Western Reserve Univ., Cleveland, OH) and their lab members were instrumental in helping RMG. learn C. elegans biology. The authors also thank Drs. Christine Campbell (UB) and Paul Mains (U. Calgary) for helpful discussions and reading the manuscript. These studies were supported in part by the National Institutes of Health grant HD34908 to RMG.

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Authors and Affiliations

  1. Dept. of Biochemistry, SUNY at Buffalo, 140 Farber Hall, 3435 Main St., Buffalo, NY, 14214, USA

    Elena Lazakovitch, Reiko Matsumoto & Richard M Gronostajski

  2. Dept. of Biology, Canisius College, Buffalo, NY, USA

    John M Kalb

  3. CREST and Gene Network Lab, National Institute of Genetics, Mishima, Japan

    Keiko Hirono & Yuji Kohara

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  1. Elena Lazakovitch
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  2. John M Kalb
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  6. Richard M Gronostajski
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Correspondence to Richard M Gronostajski.

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Authors' contributions

EL isolated the nfi-1 deletion strain and most transgene plasmids, performed most transgenic generation and phenotype characterization, and helped draft the manuscript. JMK performed microinjections for transgenic rescue and some phenotype characterization and contributed to manuscript preparation. RM performed QPCR. KH performed in situ hybridizations and β gal staining. YK identified specific cell types in which nfi-1 is expressed. RMG initiated the project and designed some of the experiments, generated some transgenics, produced vectors and proteins used in the study, and finalized preparation of the manuscript.

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Lazakovitch, E., Kalb, J.M., Matsumoto, R. et al. nfi-1 affects behavior and life-span in C. elegansbut is not essential for DNA replication or survival. BMC Dev Biol 5, 24 (2005). https://doi.org/10.1186/1471-213X-5-24

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Keywords

BMC Developmental Biology

ISSN: 1471-213X

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