Figure 6
A) Egg-laying defect in nfi-1 mutants and transgenic rescue. Bagging was measured in wild-type N2, nfi-1, N2 worms carrying the transgenic array qaEx507(N2 qaEx507) and nfi-1 worms carrying this array (nfi-1 qaEx507). Bars represent % of bagging as the mean of 3–4 independent experiments and error bars show the standard deviation. 30–75 worms of each genotype were scored in each independent experiment. N2 and N2 qaEx507showed <5% bagging. The nfi-1 mutant worms showed ~30% bagging while the rescued nfi-1 qaEx507showed <5% bagging. B) Shortened life span in nfi-1 mutants and transgenic rescue. Survival curves for the strains described above N2 (n = 57), nfi-1 (n = 58), N2 qaEx507 (n = 52) and nfi-1 qaEx507 (n = 31) are shown. Kaplan-Meier analysis (SPSS11 software) was use to determine median, percentile and p values (log rank test) and Excel was used to construct survival curves. The array generated ~50% rescue of the life-span. The experiment was repeated twice with similar results.