The developmental basis of mesenchymal stem/stromal cells (MSCs)
© Sheng. 2015
Received: 17 April 2015
Accepted: 13 November 2015
Published: 20 November 2015
Mesenchymal Stem/Stromal Cells (MSCs) define a population of progenitor cells capable of giving rises to at least three mesodermal lineages in vitro, the chondrocytes, osteoblasts and adipocytes. The validity of MSCs in vivo has been questioned because their existence, either as a homogeneous progenitor cell population or as a stem cell lineage, has been difficult to prove. The wide use of primary MSCs in regenerative and therapeutic applications raises ethical and regulatory concerns in many countries. In contrast to hematopoietic stem cells, a parallel concept which carries an embryological emphasis from its outset, MSCs have attracted little interest among developmental biologists and the embryological basis for their existence, or lack thereof, has not been carefully evaluated.
This article provides a brief, embryological overview of these three mesoderm cell lineages and offers a framework of ontological rationales for the potential existence of MSCs in vivo.
Emphasis is given to the common somatic lateral plate mesoderm origin of the majority of body’s adipose and skeletal tissues and of the major sources used for MSC derivation clinically. Support for the MSC hypothesis also comes from a large body of molecular and lineage analysis data in vivo.
It is concluded that despite the lack of a definitive proof, the MSC concept has a firm embryological basis and that advances in MSC research can be facilitated by achieving a better integration with developmental biology.
The concept of mesenchymal stem/stromal cells (MSCs) [1, 2], also referred to as skeletal stem cells  or adipose stem cells , was first introduced by Alexander Friedenstein about half a century ago. Building on the hematopoietic stem cell (HSC) work pioneered by another Russian scientist Alexander Maximow, Friedenstein described a population of bone marrow derived cells which are distinct from the HSC population and are osteogenic in vivo and clonogenic in vitro [5–7]. These bone marrow-derived MSCs were later shown to be able to self-renew, form colonies and differentiate into a multitude of mesodermal cell types in vitro . MSC populations with similar multi-lineage differentiation potentials in vitro have since been obtained from many non-bone marrow tissues , including the adipose tissue [10, 11], amniotic fluid [12, 13], placenta , umbilical cord [15–17] and peripheral blood . Clinical relevance of MSCs has been highlighted by their capacity for in vivo differentiation and engraftment and by their efficacy in promoting wound healing, tissue regeneration and immunosuppression [19–25].
Common for a field attracting a wide scope of interest from researchers, MSC biology has witnessed confusions and controversies concerning its name, definition, isolation and characterization criteria, in vivo relevance, and institutional and ethical regulations of its clinical use. In an attempt to standardize studies in this field, the International Society for Cellular Therapy came up with guidelines in 2006 for MSC characterization . The name “multipotent mesenchymal stromal cells” was preferred and three minimal criteria were outlined: 1) being plastic-adherent in culture; 2) exhibiting a set combination of surface antigens (CD73+, CD90+, CD105+, CD34-, CD45-, CD11b-, CD14-, CD19-, CD79a- and HLA-DR-); and 3) being able to differentiate in vitro into osteoblasts, chondrocytes and adipocytes. These standards, however, have not been widely adopted and criteria for MSC isolation and identification continue to vary, making cross-study comparison difficult [3, 26–31]. As a consequence, physiological nature of their therapeutic effect and cellular and molecular nature of their differentiation potentials in vivo remain ill-characterized.
This article will take an embryological approach to evaluate the evidence for the possible existence of MSCs in vivo. From the perspectives of both cell lineage specification and mesoderm germ layer patterning, developmental ontogeny of the three main mesoderm cell types of concern to the MSC biology, the adipocytes, osteoblasts and chondrocytes, will be discussed in detail. The evidence for multi-potential progenitor cell populations from molecular and lineage analysis studies in vivo will be examined. Conceptual differences between mesenchymal stem cells and mesenchymal stromal cells and between mesenchymal stem cells and mesodermal stem cells will also be compared in the broader context of stem cell biology.
Results and discussion
Except for a small, cephalic neural crest-derived population in the head, all adipocytes in the adult body are of the mesoderm origin [32, 33]. Based on their morphology and location, adipocytes are categorized as either of a white or brown adipose tissue type (WAT and BAT, respectively) [34–38]. WAT adipocytes function as energy store and BAT adipocytes as heat dissipater. A third, minor type (brite or beige adipocytes) exhibits an intermediate feature with their location associated with WATs and their function resembling BAT adipocytes . It is noteworthy that cellular and molecular features associated with mammalian adipocytes, e.g., regulated fusion of cytoplasmic lipid droplets through perilipins and respiratory uncoupling of lipid breakdown through mitochondrial uncoupling protein UCPs, are evolutionarily ancient and are present not only in adipocytes, but also in other mesoderm cell lineages and in cells derived from other germ layers [40–43]. The WAT adipocytes, of relevance to the MSC biology, are further divided into visceral and subcutaneous subtypes [34–38]. The visceral WATs have recently been shown to come from the splanchnic/visceral lateral plate mesoderm (LPM) . The subcutaneous WATs, which constitute the bulk of human body fat and are found mainly in the abdominal and gluteofemoral regions, are primarily derived from the somatic/parietal LPM (discussed in more detail below). Understanding WAT ontogeny and adipogenesis in development therefore requires proper understanding of the LPM.
Chondrogenesis and osteogenesis
These are two separate, but tightly-linked skeletogenic processes. Neural crest-derived cells make a significant contribution to the cranial bones and cartilages [45–47]. All other skeletal elements are of mesoderm origin [48, 49]. With the exception of the clavicle, which is generated through a mixture of intramembranous and endochondrial ossification , all post-cranial bones form through endochondrial ossification, i.e., secretion of bone-specific matrix proteins and subsequent mineralization of this matrix take place in a tissue architecture modeled by the chondrocytes. Therefore, percentage-wise, most cartilaginous tissues in the embryo exist only temporarily. Three mesoderm lineages in development, the axial, paraxial and lateral plate, are capable of generating skeletal elements. The axial mesoderm gives rise to the embryonic notochord and the adult nucleus pulposus and expresses many cartilage-specific markers (e.g., type II collagen and aggrecan) [51, 52]. However, cellular morphogenesis in the notochord and the nucleus pulposus is very different from what is known for the cartilage and these axial mesoderm-derived cells are generally not considered to be chondrocytes. The paraxial/somitic mesoderm generates all axial and associated skeletal elements (the vertebrae, ribs and part of the shoulder girdle), whereas all distal skeletal elements (bones in the limbs, the pelvic girdle, the sternum and part of the shoulder girdle) are derived from the somatic/parietal layer of the LPM [53, 54]. In endochondrial bones, chondrogenic differentiation of osteochondrogenic progenitors proceeds first, and osteogenic differentiation takes place later from a pool of quiescent progenitors located on the cartilage surface (perichondrium) . Recent reports suggested that osteogenesis can also take place through chondrocyte de-differentiation or transdifferentiation [56, 57].
Major lineages of the mesoderm germ layer
The LPM and its somatic and splanchnic layers
In vivo evidence for multipotential progenitors in the somatic layer of the LPM
The ontological evidence discussed above suggests the three cell types of interest to the MSC biology have the somatic LPM as their common developmental origin. It is also evident that the majority of the bone and adipose tissues that are used as clinical sources for MSC enrichment/purification in vitro are derived from the somatic LPM. Although differentiation of MSCs in vitro may not necessarily mimic their in vivo behavior, it is worthwhile first reviewing the in vivo evidence for the existence of MSC-like progenitor/stem cell populations in the somatic LPM.
The somatic LPM, after its formation but before its differentiation, expresses many genes (e.g., αSMA) which are considered to be MSC markers later on [67–71]. Inducible αSMA-Cre-mediated lineage labeling suggested that αSMA positive cells in the bone marrow and periosteum are positive for MSC markers and these cells can give rise to chondrocytes and osteoblasts during fracture repair in vivo and to multiple mesenchymal lineages in vitro . Furthermore, αSMA also marks an adult adipose stem cell population  which resembles adipose and bone marrow MSCs with multilineage differentiation potential in vitro [70, 71]. TWIST1, another marker for early somatic LPM , is highly expressed together with its close homolog TWIST2 in primary MSCs [75, 76]. TWIST1 and TWIST2 expression prevents MSC differentiation in vivo [75, 76] and TWIST2-positive cells were shown to give rise to both osteoblasts and chondrocytes in vivo in a Cre-mediated lineage analysis .
The epithelial-shaped early somatic LPM cells differentiate by undergoing EMT and generate a population of mesenchymal cells located between the overlying ectoderm and the remaining epithelial somatic LPM (the future parietal mesothelium). This population of somatic LPM-derived mesenchymal cells is present throughout the antero-posterior axis of the embryo, but is most prominent in areas where limb buds develop . These mesenchymal cells are initially homogenous and at the limb levels they are uniformly positive for Prx1, a paired-related homeobox gene . Results from virus-mediated single-cell labeling of the limb mesenchyme suggest that these are multi-potential cells capable of giving rise to two or more of the limb cell types, including those in the cartilage, perichondrium, tendon, muscle connective and dermis . Sox9, an early molecular marker for chondrogenic condensation, labels in fact a multi-lineage sub-population of these mesenchymal cells, including those contributing to the cartilage, bone, tendon and ligament [81, 82]. Regulated by a pre-adipocyte marker Pref-1, Sox9 also inhibits the adipogenic potential of these mesenchymal cells .
In a more differentiated tissue environment derived from the somatic LPM, multi-potential progenitors have been shown to exist for the osteoblasts, adipocytes and perivascular stromal cells by Osterix-Cre mediated lineage labeling , for the osteoblasts, chondrocytes, endothelial and bone marrow stromal cells by Nestin-Cre mediated lineage analysis [27, 85], for the osteoblasts, chondrocytes and marrow stromal cells by Gremlin1-Cre mediated lineage and Rainbow Actin-Cre mediated clonal analyses [86, 87], for the chondrocytes and osteoblasts by Col2-Cre mediated lineage labeling , and for the osteoblasts and adipocytes by fate-mapping and transplantation analyses of leptinR+ cells in the adult bone marrow .
Mesenchymal stem cells or mesenchymal stromal cells
Taken together, data from the ontological, molecular and cellular analyses strongly support the hypothesis that there exists a MSC-like progenitor population both during somatic LPM differentiation and during homeostatic maintenance of somatic LPM-derived tissues. The multi-potential stemness of MSCs in vivo, with the capacity to generate tissues including the bone, cartilage, tendon, muscle, fat and marrow stroma , however, has not been satisfactorily demonstrated. This gap can be viewed from three perspectives in the broader context of stem cell biology.
Mesenchymal stem cells or mesodermal stem cells
Although most of the cell and tissue types which have been associated with MSCs come ontologically from the somatic LPM, many of them do not. For example, as target differentiation lineages, the cardiac and most of the smooth muscles come from the splanchnic LPM and the skeletal muscles are from the somitic mesoderm. As tissue sources, the neural crest is of ectoderm origin, and axial bones and dorsal dermis are of somitic mesoderm origin. The peripheral blood in the adult and the umbilical cord blood in the fetus are of splanchnic LPM/splanchnic extraembryonic mesoderm origin, the amniotic mesoderm is of somatic extraembryonic mesoderm origin and the placental and the umbilical cord mesenchyme is derived from a mixture of somatic and splanchnic extraembryonic mesoderm cells.
Leaving aside the neural crest-derived cell populations (not discussed here), one may ask whether the term “mesodermal stem cells” is more suitable to describe the properties associated with the MSCs. To answer this question one needs to take a fresh look at the epigenetic landscape proposed by Waddington about 60 years ago . The original concept of epigenetic landscape of animal development outlined progressive restriction of differentiation potentials from a totipotent zygote to terminally differentiated functional cell types. Uni-, bi- or multi-potential stem cells are perceived to exist in each branch or at the branching point. Cell lineages that are closely related ontologically are more likely to be represented by a common progenitor/stem cell population and those distantly related less likely so. For example, paths and barriers for the germ cells and the soma and for the three principal germ layers are set early in development and fate conversion crossing these barriers is almost never observed in vivo. One may therefore argue that the MSCs are a sub-population of, and are distinct from, a pan-mesodermal stem cell population, the latter of which can be viewed as the equivalent of primitive streak-like mesoderm progenitors in vivo (Fig. 3). Although other sub-populations of lineage-biased, multi-potential mesodermal stem cells may exist, cell types not strictly derived with the somatic LPM should preferably not be associated with the MSCs.
Advances from iPSC-related research, however, have complicated such a simplistic paradigm. Reversion from a terminally differentiated fate to a pluripotent fate can now be achieved relatively easily through molecular or chemical perturbations of this epigenetic landscape and paths of reprogramming may not necessarily mirror the conventional landscape of progressive differentiation in vivo. As a consequence, it is unclear whether the knowledge obtained from in vivo studies should serve as the guiding principle for achieving fate reversion and targeted lineage differentiation in vitro. It will be fascinating to see whether the epigenetic landscape manifested in vitro can teach us about unrealized potentials in vivo. MSC studies in vitro therefore should not be confined by MSC behaviors in vivo.
This article has outlined the ontological, molecular and cellular evidence in support of the existence of MSCs in vivo. The somatic LPM is the most important mesoderm compartment for the cells and tissues commonly associated with MSCs. The term “mesenchymal stem cells” is preferred by this author and a clear distinction should be made between somatic LPM-derived MSCs and mesenchymal-shaped stem-like cells derived from other mesodermal compartments. Such understanding of MSCs based on in vivo evidence would benefit in vitro endeavors in harnessing the therapeutic powers of MSCs. The ultimate goal of MSC-related research is to integrate in vitro-reconstituted cells or tissues into an in vivo environment, which will be facilitated by an awareness of how endogenous MSC populations differentiate and self-organize in vivo.
Views expressed in this article are based partially on research carried out in the author's laboratory at RIKEN Center for Developmental Biology. All animal experiments performed in the author's laboratory were approved by the institute's ethical committee.
I would like to thank RIKEN for financial support and past and current members of my laboratory for the concepts presented in this article. The author would also like to thank Dr. Wei Weng for critical reading of the manuscript.
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- Dominici M, Le Blanc K, Mueller I, Slaper-Cortenbach I, Marini FC, Krause DS, et al. Minimal criteria for defining multipotent mesenchymal stromal cells. The international society for cellular therapy position statement. Cytotherapy. 2006;8(4):315–7.PubMedView ArticleGoogle Scholar
- Caplan AI. Mesenchymal stem cells. J Orthop Res. 1991;9(5):641–50.PubMedView ArticleGoogle Scholar
- Bianco P. “Mesenchymal” stem cells. Annu Rev Cell Dev Biol. 2014;30(2014):677–704.PubMedView ArticleGoogle Scholar
- Cawthorn WP, Scheller EL, MacDougald OA. Adipose tissue stem cells meet preadipocyte commitment: going back to the future. J Lipid Res. 2012;53(2):227–46.PubMed CentralPubMedView ArticleGoogle Scholar
- Friedenstein AJ, Piatetzky II S, Petrakova KV. Osteogenesis in transplants of bone marrow cells. J Embryol Exp Morphol. 1966;16(3):381–90.PubMedGoogle Scholar
- Friedenstein AJ, Petrakova KV, Kurolesova AI, Frolova GP. Heterotopic of bone marrow. Analysis of precursor cells for osteogenic and hematopoietic tissues. Transplantation. 1968;6(2):230–47.PubMedView ArticleGoogle Scholar
- Afanasyev BV, Elstner EE, Zander AR. A. J. Friedenstein, founder of the mesenchymal stem cell concept. Cell Ther Transplant. 2009:1(35–38)Google Scholar
- Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, et al. Multilineage potential of adult human mesenchymal stem cells. Science. 1999;284(5411):143–7.PubMedView ArticleGoogle Scholar
- Williams AR, Hare JM. Mesenchymal stem cells: biology, pathophysiology, translational findings, and therapeutic implications for cardiac disease. Circ Res. 2011;109(8):923–40.PubMed CentralPubMedView ArticleGoogle Scholar
- Zuk PA, Zhu M, Ashjian P, De Ugarte DA, Huang JI, Mizuno H, et al. Human adipose tissue is a source of multipotent stem cells. Mol Biol Cell. 2002;13(12):4279–95.PubMed CentralPubMedView ArticleGoogle Scholar
- Gimble J, Guilak F. Adipose-derived adult stem cells: isolation, characterization, and differentiation potential. Cytotherapy. 2003;5(5):362–9.PubMedView ArticleGoogle Scholar
- Anker PS I’t, Scherjon SA, Kleijburg-van der Keur C, Noort WA, Claas FH, Willemze R, et al. Amniotic fluid as a novel source of mesenchymal stem cells for therapeutic transplantation. Blood. 2003;102(4):1548–9.View ArticleGoogle Scholar
- De Coppi P, Bartsch Jr G, Siddiqui MM, Xu T, Santos CC, Perin L, et al. Isolation of amniotic stem cell lines with potential for therapy. Nat Biotechnol. 2007;25(1):100–6.PubMedView ArticleGoogle Scholar
- Vellasamy S, Sandrasaigaran P, Vidyadaran S, George E, Ramasamy R. Isolation and characterisation of mesenchymal stem cells derived from human placenta tissue. World J Stem Cells. 2012;4(6):53–61.PubMed CentralPubMedView ArticleGoogle Scholar
- Lee OK, Kuo TK, Chen WM, Lee KD, Hsieh SL, Chen TH. Isolation of multipotent mesenchymal stem cells from umbilical cord blood. Blood. 2004;103(5):1669–75.PubMedView ArticleGoogle Scholar
- Wang HS, Hung SC, Peng ST, Huang CC, Wei HM, Guo YJ, et al. Mesenchymal stem cells in the Wharton’s jelly of the human umbilical cord. Stem Cells. 2004;22(7):1330–7.PubMedView ArticleGoogle Scholar
- Tondreau T, Meuleman N, Delforge A, Dejeneffe M, Leroy R, Massy M, et al. Mesenchymal stem cells derived from CD133-positive cells in mobilized peripheral blood and cord blood: proliferation, Oct4 expression, and plasticity. Stem Cells. 2005;23(8):1105–12.PubMedView ArticleGoogle Scholar
- Zvaifler NJ, Marinova-Mutafchieva L, Adams G, Edwards CJ, Moss J, Burger JA, et al. Mesenchymal precursor cells in the blood of normal individuals. Arthritis Res. 2000;2(6):477–88.PubMed CentralPubMedView ArticleGoogle Scholar
- Karp JM, Leng Teo GS. Mesenchymal stem cell homing: the devil is in the details. Cell Stem Cell. 2009;4(3):206–16.PubMedView ArticleGoogle Scholar
- Sivanathan KN, Gronthos S, Rojas-Canales D, Thierry B, Coates PT. Interferon-gamma modification of mesenchymal stem cells: implications of autologous and allogeneic mesenchymal stem cell therapy in allotransplantation. Stem Cell Rev. 2014;10(3):351–75.PubMedView ArticleGoogle Scholar
- Nemeth K, Keane-Myers A, Brown JM, Metcalfe DD, Gorham JD, Bundoc VG, et al. Bone marrow stromal cells use TGF-beta to suppress allergic responses in a mouse model of ragweed-induced asthma. Proc Natl Acad Sci U S A. 2010;107(12):5652–7.PubMed CentralPubMedView ArticleGoogle Scholar
- Ren G, Zhang L, Zhao X, Xu G, Zhang Y, Roberts AI, et al. Mesenchymal stem cell-mediated immunosuppression occurs via concerted action of chemokines and nitric oxide. Cell Stem Cell. 2008;2(2):141–50.PubMedView ArticleGoogle Scholar
- English K, French A, Wood KJ. Mesenchymal stromal cells: facilitators of successful transplantation? Cell Stem Cell. 2010;7(4):431–42.PubMedView ArticleGoogle Scholar
- Murphy MB, Moncivais K, Caplan AI. Mesenchymal stem cells: environmentally responsive therapeutics for regenerative medicine. Exp Mol Med. 2013;45:e54.PubMed CentralPubMedView ArticleGoogle Scholar
- Tyndall A. Mesenchymal stem cell treatments in rheumatology: a glass half full? Nat Rev Rheumatol. 2014;10(2):117–24.PubMedView ArticleGoogle Scholar
- Al-Nbaheen M, Vishnubalaji R, Ali D, Bouslimi A, Al-Jassir F, Megges M, et al. Human stromal (mesenchymal) stem cells from bone marrow, adipose tissue and skin exhibit differences in molecular phenotype and differentiation potential. Stem Cell Rev. 2013;9(1):32–43.PubMed CentralPubMedView ArticleGoogle Scholar
- Mendez-Ferrer S, Michurina TV, Ferraro F, Mazloom AR, Macarthur BD, Lira SA, et al. Mesenchymal and haematopoietic stem cells form a unique bone marrow niche. Nature. 2010;466(7308):829–34.PubMed CentralPubMedView ArticleGoogle Scholar
- Wetzig A, Alaiya A, Al-Alwan M, Pradez CB, Pulicat MS, Al-Mazrou A, et al. Differential marker expression by cultures rich in mesenchymal stem cells. BMC Cell Biol. 2013;14:54.PubMed CentralPubMedView ArticleGoogle Scholar
- Bianco P, Cao X, Frenette PS, Mao JJ, Robey PG, Simmons PJ, et al. The meaning, the sense and the significance: translating the science of mesenchymal stem cells into medicine. Nat Med. 2013;19(1):35–42.PubMed CentralPubMedView ArticleGoogle Scholar
- Feisst V, Brooks AE, Chen CJ, Dunbar PR. Characterization of mesenchymal progenitor cell populations directly derived from human dermis. Stem Cells Dev. 2014;23(6):631–42.PubMedView ArticleGoogle Scholar
- Keating A. Mesenchymal stromal cells: new directions. Cell Stem Cell. 2012;10(6):709–16.PubMedView ArticleGoogle Scholar
- Billon N, Iannarelli P, Monteiro MC, Glavieux-Pardanaud C, Richardson WD, Kessaris N, et al. The generation of adipocytes by the neural crest. Development. 2007;134(12):2283–92.PubMedView ArticleGoogle Scholar
- Sowa Y, Imura T, Numajiri T, Takeda K, Mabuchi Y, Matsuzaki Y, et al. Adipose stromal cells contain phenotypically distinct adipogenic progenitors derived from neural crest. PLoS One. 2013;8(12):e84206.PubMed CentralPubMedView ArticleGoogle Scholar
- Cristancho AG, Lazar MA. Forming functional fat: a growing understanding of adipocyte differentiation. Nat Rev Mol Cell Biol. 2011;12(11):722–34.PubMedView ArticleGoogle Scholar
- Berry DC, Stenesen D, Zeve D, Graff JM. The developmental origins of adipose tissue. Development. 2013;140(19):3939–49.PubMed CentralPubMedView ArticleGoogle Scholar
- Frontini A, Cinti S. Distribution and development of brown adipocytes in the murine and human adipose organ. Cell Metab. 2010;11(4):253–6.PubMedView ArticleGoogle Scholar
- Rosen ED, Spiegelman BM. What we talk about when we talk about fat. Cell. 2014;156(1–2):20–44.PubMed CentralPubMedView ArticleGoogle Scholar
- Gesta S, Tseng YH, Kahn CR. Developmental origin of fat: tracking obesity to its source. Cell. 2007;131(2):242–56.PubMedView ArticleGoogle Scholar
- Long JZ, Svensson KJ, Tsai L, Zeng X, Roh HC, Kong X, et al. A smooth muscle-like origin for beige adipocytes. Cell Metab. 2014;19(5):810–20.PubMed CentralPubMedView ArticleGoogle Scholar
- Brasaemle DL, Wolins NE. Packaging of fat: an evolving model of lipid droplet assembly and expansion. J Biol Chem. 2012;287(4):2273–9.PubMed CentralPubMedView ArticleGoogle Scholar
- Ohsaki Y, Suzuki M, Fujimoto T. Open questions in lipid droplet biology. Chem Biol. 2014;21(1):86–96.PubMedView ArticleGoogle Scholar
- Ottaviani E, Malagoli D, Franceschi C. The evolution of the adipose tissue: a neglected enigma. Gen Comp Endocrinol. 2011;174(1):1–4.PubMedView ArticleGoogle Scholar
- Li Y, Lasar D, Fromme T, Klingenspor M. White, brite, and brown adipocytes: the evolution and function of a heater organ in mammals. Can J Zool. 2014;92:615–26.View ArticleGoogle Scholar
- Chau YY, Bandiera R, Serrels A, Martinez-Estrada OM, Qing W, Lee M, et al. Visceral and subcutaneous fat have different origins and evidence supports a mesothelial source. Nat Cell Biol. 2014;16(4):367–75.PubMed CentralPubMedView ArticleGoogle Scholar
- Noden DM, Trainor PA. Relations and interactions between cranial mesoderm and neural crest populations. J Anat. 2005;207(5):575–601.PubMed CentralPubMedView ArticleGoogle Scholar
- Bronner ME, LeDouarin NM. Development and evolution of the neural crest: an overview. Dev Biol. 2012;366(1):2–9.PubMed CentralPubMedView ArticleGoogle Scholar
- Gross JB, Hanken J. Review of fate-mapping studies of osteogenic cranial neural crest in vertebrates. Dev Biol. 2008;317(2):389–400.PubMedView ArticleGoogle Scholar
- Lefebvre V, Bhattaram P. Vertebrate skeletogenesis. Curr Top Dev Biol. 2010;90:291–317.PubMed CentralPubMedView ArticleGoogle Scholar
- Zhang G, Eames BF, Cohn MJ. Chapter 2. Evolution of vertebrate cartilage development. Curr Top Dev Biol. 2009;86:15–42.PubMedView ArticleGoogle Scholar
- Hall BK. Development of the clavicles in birds and mammals. J Exp Zool. 2001;289(3):153–61.PubMedView ArticleGoogle Scholar
- Stemple DL. Structure and function of the notochord: an essential organ for chordate development. Development. 2005;132(11):2503–12.PubMedView ArticleGoogle Scholar
- Rodrigues-Pinto R, Richardson SM, Hoyland JA. Identification of novel nucleus pulposus markers: interspecies variations and implications for cell-based therapiesfor intervertebral disc degeneration. Bone Joint Res. 2013;2(8):169–78.PubMed CentralPubMedView ArticleGoogle Scholar
- Christ B, Huang R, Scaal M. Amniote somite derivatives. Dev Dyn. 2007;236(9):2382–96.PubMedView ArticleGoogle Scholar
- Malashichev Y, Christ B, Prols F. Avian pelvis originates from lateral plate mesoderm and its development requires signals from both ectoderm and paraxial mesoderm. Cell Tissue Res. 2008;331(3):595–604.PubMedView ArticleGoogle Scholar
- Long F. Building strong bones: molecular regulation of the osteoblast lineage. Nat Rev Mol Cell Biol. 2012;13(1):27–38.View ArticleGoogle Scholar
- Zhou X, von der Mark K, Henry S, Norton W, Adams H, de Crombrugghe B. Chondrocytes transdifferentiate into osteoblasts in endochondral bone during development, postnatal growth and fracture healing in mice. PLoS Genet. 2014;10(12):e1004820.PubMed CentralPubMedView ArticleGoogle Scholar
- Yang L, Tsang KY, Tang HC, Chan D, Cheah KS. Hypertrophic chondrocytes can become osteoblasts and osteocytes in endochondral bone formation. Proc Natl Acad Sci U S A. 2014;111(33):12097–102.PubMed CentralPubMedView ArticleGoogle Scholar
- Alev C, Wu Y, Kasukawa T, Jakt LM, Ueda HR, Sheng G. Transcriptomic landscape of the primitive streak. Development. 2010;137(17):2863–74.PubMedView ArticleGoogle Scholar
- Kimelman D. Mesoderm induction: from caps to chips. Nat Rev Genet. 2006;7(5):360–72.PubMedView ArticleGoogle Scholar
- Nakaya Y, Sheng G. An amicable separation: chick’s way of doing EMT. Cell Adh Migr. 2009;3(2):160–3.PubMed CentralPubMedView ArticleGoogle Scholar
- Alev C, Wu Y, Nakaya Y, Sheng G. Decoupling of amniote gastrulation and streak formation reveals a morphogenetic unity in vertebrate mesoderm induction. Development. 2013;140(13):2691–6.PubMedView ArticleGoogle Scholar
- Nakaya Y, Sukowati EW, Wu Y, Sheng G. RhoA and microtubule dynamics control cell-basement membrane interaction in EMT during gastrulation. Nat Cell Biol. 2008;10(7):765–75.PubMedView ArticleGoogle Scholar
- Nakaya Y, Sukowati EW, Sheng G. Epiblast integrity requires CLASP and Dystroglycan-mediated microtubule anchoring to the basal cortex. J Cell Biol. 2013;202(4):637–51.PubMed CentralPubMedView ArticleGoogle Scholar
- Tam PP, Williams EA, Chan WY. Gastrulation in the mouse embryo: ultrastructural and molecular aspects of germ layer morphogenesis. Microsc Res Tech. 1993;26(4):301–28.PubMedView ArticleGoogle Scholar
- Tam PP, Beddington RS. The formation of mesodermal tissues in the mouse embryo during gastrulation and early organogenesis. Development. 1987;99(1):109–26.PubMedGoogle Scholar
- Nowotschin S, Hadjantonakis AK. Cellular dynamics in the early mouse embryo: from axis formation to gastrulation. Curr Opin Genet Dev. 2010;20(4):420–7.PubMed CentralPubMedView ArticleGoogle Scholar
- Shin M, Nagai H, Sheng G. Notch mediates Wnt and BMP signals in the early separation of smooth muscle progenitors and blood/endothelial common progenitors. Development. 2009;136(4):595–603.PubMedView ArticleGoogle Scholar
- Dyment NA, Hagiwara Y, Matthews BG, Li Y, Kalajzic I, Rowe DW. Lineage tracing of resident tendon progenitor cells during growth and natural healing. PLoS One. 2014;9(4):e96113.PubMed CentralPubMedView ArticleGoogle Scholar
- Crisan M, Yap S, Casteilla L, Chen CW, Corselli M, Park TS, et al. A perivascular origin for mesenchymal stem cells in multiple human organs. Cell Stem Cell. 2008;3(3):301–13.PubMedView ArticleGoogle Scholar
- Kalajzic Z, Li H, Wang LP, Jiang X, Lamothe K, Adams DJ, et al. Use of an alpha-smooth muscle actin GFP reporter to identify an osteoprogenitor population. Bone. 2008;43(3):501–10.PubMed CentralPubMedView ArticleGoogle Scholar
- Cai X, Lin Y, Hauschka PV, Grottkau BE. Adipose stem cells originate from perivascular cells. Biol Cell. 2011;103(9):435–47.PubMedView ArticleGoogle Scholar
- Grcevic D, Pejda S, Matthews BG, Repic D, Wang L, Li H, et al. In vivo fate mapping identifies mesenchymal progenitor cells. Stem Cells. 2012;30(2):187–96.PubMed CentralPubMedView ArticleGoogle Scholar
- Jiang Y, Berry DC, Tang W, Graff JM. Independent stem cell lineages regulate adipose organogenesis and adipose homeostasis. Cell Rep. 2014;9(3):1007–22.PubMed CentralPubMedView ArticleGoogle Scholar
- Fuchtbauer EM. Expression of M-twist during postimplantation development of the mouse. Dev Dyn. 1995;204(3):316–22.PubMedView ArticleGoogle Scholar
- Isenmann S, Arthur A, Zannettino AC, Turner JL, Shi S, Glackin CA, et al. TWIST family of basic helix-loop-helix transcription factors mediate human mesenchymal stem cell growth and commitment. Stem Cells. 2009;27(10):2457–68.PubMedView ArticleGoogle Scholar
- Yang DC, Yang MH, Tsai CC, Huang TF, Chen YH, Hung SC. Hypoxia inhibits osteogenesis in human mesenchymal stem cells through direct regulation of RUNX2 by TWIST. PLoS One. 2011;6(9):e23965.PubMed CentralPubMedView ArticleGoogle Scholar
- Yu K, Xu J, Liu Z, Sosic D, Shao J, Olson EN, et al. Conditional inactivation of FGF receptor 2 reveals an essential role for FGF signaling in the regulation of osteoblast function and bone growth. Development. 2003;130(13):3063–74.PubMedView ArticleGoogle Scholar
- Gros J, Tabin CJ. Vertebrate limb bud formation is initiated by localized epithelial-to-mesenchymal transition. Science. 2014;343(6176):1253–6.PubMed CentralPubMedView ArticleGoogle Scholar
- Logan M, Martin JF, Nagy A, Lobe C, Olson EN, Tabin CJ. Expression of Cre Recombinase in the developing mouse limb bud driven by a Prxl enhancer. Genesis. 2002;33(2):77–80.PubMedView ArticleGoogle Scholar
- Pearse 2nd RV, Scherz PJ, Campbell JK, Tabin CJ. A cellular lineage analysis of the chick limb bud. Dev Biol. 2007;310(2):388–400.PubMed CentralPubMedView ArticleGoogle Scholar
- Akiyama H, Kim JE, Nakashima K, Balmes G, Iwai N, Deng JM, et al. Osteo-chondroprogenitor cells are derived from Sox9 expressing precursors. Proc Natl Acad Sci U S A. 2005;102(41):14665–70.PubMedView ArticleGoogle Scholar
- Sugimoto Y, Takimoto A, Akiyama H, Kist R, Scherer G, Nakamura T, et al. Scx+/Sox9+ progenitors contribute to the establishment of the junction between cartilage and tendon/ligament. Development. 2013;140(11):2280–8.PubMedView ArticleGoogle Scholar
- Wang Y, Sul HS. Pref-1 regulates mesenchymal cell commitment and differentiation through Sox9. Cell Metab. 2009;9(3):287–302.PubMed CentralPubMedView ArticleGoogle Scholar
- Liu Y, Strecker S, Wang L, Kronenberg MS, Wang W, Rowe DW, et al. Osterix-cre labeled progenitor cells contribute to the formation and maintenance of the bone marrow stroma. PLoS One. 2013;8(8):e71318.PubMed CentralPubMedView ArticleGoogle Scholar
- Ono N, Ono W, Mizoguchi T, Nagasawa T, Frenette PS, Kronenberg HM. Vasculature-associated cells expressing nestin in developing bones encompass early cells in the osteoblast and endothelial lineage. Dev Cell. 2014;29(3):330–9.PubMed CentralPubMedView ArticleGoogle Scholar
- Worthley DL, Churchill M, Compton JT, Tailor Y, Rao M, Si Y, et al. Gremlin 1 identifies a skeletal stem cell with bone, cartilage, and reticular stromal potential. Cell. 2015;160(1–2):269–84.PubMedView ArticleGoogle Scholar
- Chan CK, Seo EY, Chen JY, Lo D, McArdle A, Sinha R, et al. Identification and specification of the mouse skeletal stem cell. Cell. 2015;160(1–2):285–98.PubMedView ArticleGoogle Scholar
- Maes C, Kobayashi T, Selig MK, Torrekens S, Roth SI, Mackem S, et al. Osteoblast precursors, but not mature osteoblasts, move into developing and fractured bones along with invading blood vessels. Dev Cell. 2010;19(2):329–44.PubMed CentralPubMedView ArticleGoogle Scholar
- Zhou BO, Yue R, Murphy MM, Peyer JG, Morrison SJ. Leptin-receptor-expressing mesenchymal stromal cells represent the main source of bone formed by adult bone marrow. Cell Stem Cell. 2014;15(2):154–68.PubMed CentralPubMedView ArticleGoogle Scholar
- Lee CC, Christensen JE, Yoder MC, Tarantal AF. Clonal analysis and hierarchy of human bone marrow mesenchymal stem and progenitor cells. Exp Hematol. 2010;38(1):46–54.PubMed CentralPubMedView ArticleGoogle Scholar
- Phinney DG. Functional heterogeneity of mesenchymal stem cells: implications for cell therapy. J Cell Biochem. 2012;113(9):2806–12.PubMedView ArticleGoogle Scholar
- Caplan AI. New era of cell-based orthopedic therapies. Tissue Eng B Rev. 2009;15(2):195–200.View ArticleGoogle Scholar
- Gokcinar-Yagci B, Uckan-Cetinkaya D, Celebi-Saltik B. Pericytes: Properties. Stem Cell Rev: Functions and Applications in Tissue Engineering; 2015.Google Scholar
- Blasi A, Martino C, Balducci L, Saldarelli M, Soleti A, Navone SE, et al. Dermal fibroblasts display similar phenotypic and differentiation capacity to fat-derived mesenchymal stem cells, but differ in anti-inflammatory and angiogenic potential. Vascular cell. 2011;3(1):5.PubMed CentralPubMedView ArticleGoogle Scholar
- Lv FJ, Tuan RS, Cheung KM, Leung VY. Concise review: the surface markers and identity of human mesenchymal stem cells. Stem Cells. 2014;32(6):1408–19.PubMedView ArticleGoogle Scholar
- Waddington CH. The strategy of the genes; a discussion of some aspects of theoretical biology. London: Allen & Unwin; 1957.Google Scholar