- Research article
- Open Access
Bunched, the Drosophilahomolog of the mammalian tumor suppressor TSC-22, promotes cellular growth
© Gluderer et al; licensee BioMed Central Ltd. 2008
- Received: 27 November 2007
- Accepted: 28 January 2008
- Published: 28 January 2008
Transforming Growth Factor-β1 stimulated clone-22 (TSC-22) is assumed to act as a negative growth regulator and tumor suppressor. TSC-22 belongs to a family of putative transcription factors encoded by four distinct loci in mammals. Possible redundancy among the members of the TSC-22/Dip/Bun protein family complicates a genetic analysis. In Drosophila, all proteins homologous to the TSC-22/Dip/Bun family members are derived from a single locus called bunched (bun).
We have identified bun in an unbiased genetic screen for growth regulators in Drosophila. Rather unexpectedly, bun mutations result in a growth deficit. Under standard conditions, only the long protein isoform BunA – but not the short isoforms BunB and BunC – is essential and affects growth. Whereas reducing bunA function diminishes cell number and cell size, overexpression of the short isoforms BunB and BunC antagonizes bunA function.
Our findings establish a growth-promoting function of Drosophila BunA. Since the published studies on mammalian systems have largely neglected the long TSC-22 protein version, we hypothesize that the long TSC-22 protein is a functional homolog of BunA in growth regulation, and that it is antagonized by the short TSC-22 protein.
- Leucine Zipper
- Short Isoforms
- Allelic Series
- Mutant Larva
- Dorsal Compartment
Tumorigenesis is frequently associated with a loss of a tumor suppressor, allowing tumor cells to become self-sufficient in growth signals, to become insensitive to growth-inhibitory signals, or to evade apoptosis (reviewed in ). Thus, the functional characterization of tumor suppressors is key to a better understanding of the signaling events leading to aberrant growth.
Transforming Growth Factor-β1 stimulated clone-22 (TSC-22) is a putative negative growth regulator and tumor suppressor in mammals. TSC-22 has first been isolated as a TGF-β1 responsive gene from a mouse osteoblastic cell line . It encodes a putative transcription factor that binds to DNA in vitro via its TSC-box . TSC-22 expression has been found to be lowered in different mouse and human tumors, including liver , brain , prostate , and salivary gland tumors . Consistently, downregulation of TSC-22 enhances growth in the salivary gland cell line TYS , whereas upregulation of TSC-22 is associated with apoptosis [8, 9] and growth inhibition . Increased TSC-22 expression also correlates with growth inhibition in primary human prostatic cancer cells [11, 12]. Furthermore, in the mammary carcinoma cell line T47D, TSC-22 is a target gene of progesterone, which is used to treat hormone dependent breast tumors . However, TSC-22 has also been found to be upregulated in renal cell carcinoma, challenging its proposed function in tumor suppression . Furthermore, most studies on the role of TSC-22 in tumor formation rely on cell culture experiments, and no information is available on the in vivo function of TSC-22 in growth regulation.
The genetic characterization of TSC-22 in mammals is hampered in two ways. First, the TSC-22 locus gives rise to two transcripts encoding a longer and a shorter isoform (TSC22D1.1 and TSC22D1.2, respectively). They share the C-terminally located TSC-box and a leucine zipper domain, but their N-termini are distinct. In most of the studies the two isoforms were not examined separately, or only the short isoform TSC22D1.2 has been analyzed. The possibility of diverse (or even antagonizing) functions of the TSC-22 isoforms has been largely neglected. Second, there are four genomic loci (TSC22D1 to TSC22D4) encoding TSC-22/Dip/Bun family members with diverse functions in mammals. All TSC-22/Dip/Bun proteins possess a TSC-box and a leucine zipper. TSC22D3 encodes three short isoforms with different N-termini, and a recent study shows that murine TSC22D3 isoforms have differential functions in cultured kidney cells . One isoform, TSC22D3.2 or Gilz (glucocorticoid-induced leucine zipper), has been investigated intensively. Gilz is induced by glucocorticoids, is highly expressed in lymphoid tissue, and plays a role in the regulation of T cell receptor mediated cell death [16–19]. Besides its function in the immune system, Gilz seems to be important for the aldosterone response and sodium homeostasis of cultured kidney cells [20, 21]. Via its N-terminus, Gilz binds to NF-kappaB , to c-Jun and c-Fos , and to Raf-1 . Furthermore, Gilz is a direct FoxO3 target gene . The function of TSC22D2 (TILZ4 = TSC-22 related inducible leucine zipper 4) is less well understood. In humans, two very similar long TSC22D2 isoforms are known [Swiss-Prot:O75157], and mice have several TSC22D2 transcripts potentially coding for short TSC22D2 isoforms with distinct N-termini . TSC22D2 is involved in the osmotic stress response of mouse kidney cells . Finally, TSC22D4 (THG-1 = TSC-22 homologous gene-1) can form heterodimers with TSC-22 (TSC22D1.2)  and is important in pituitary development in mice . Since the potential redundancy among the various TSC-22/Dip/Bun family members renders a genetic analysis in mammals very difficult, it is important to assess the in vivo function of TSC-22 in a simpler model organism.
Drosophila melanogaster is a suitable model organism to study growth regulation. For example, the involvement of insulin signaling [29–31] or of the proto-oncogene dMyc  in growth control has been genetically analyzed in Drosophila. In addition, screens for genes restricting growth have identified the Hippo-Salvador-Warts signaling cassette that may also have a tumor suppressor function in humans [33, 34]. The Drosophila genome contains a single gene, bunched (bun), that encodes proteins homologous to the TSC-22/Dip/Bun family members. bun has been found to influence the development of the embryonic peripheral nervous system , to be expressed during eye development , and to be required for proper oogenesis . Like TSC-22/Dip/bun genes in mammals, the Drosophila bun gene gives rise to alternatively spliced transcripts (six different transcripts, bun-RA to bun-RF), and little is known about the functions of the individual proteins so far.
Here we report that bun functions as a positive growth regulator in Drosophila. In a tissue-specific screen for genes involved in growth control, we have isolated eight bun alleles. We demonstrate that only the long Bun isoform, BunA/F, promotes cellular growth.
Identification of bunas a positive growth regulator
The genomic locus of bun spans 90 kb and comprises at least 12 (partially overlapping) exons (Figure 1E). Based on the existence of ESTs, the bun locus gives rise to at least six different transcripts (bun-RA – bun-RF) . Since the bunD-F transcripts have been annotated only recently, our study mainly focused on bunA-C. bunA and bunF have largely overlapping ORFs, but the BunF protein lacks the first 109 N-terminal amino acids present in BunA. The six bun transcripts have distinct promoter regions and code for six putative transcription factors that contain a DNA-binding domain called TSC-box and an adjacent leucine zipper that likely serves as a dimerization domain (Figure 1D). Proteins of the TSC-22/Dip/Bun family are found in various organisms ranging from C. elegans to mammals. Apart from the TSC-box and the leucine zipper, the amino acid sequences of the Drosophila Bun proteins are poorly conserved when compared to their mammalian homologs. However, for long TSC-22/Dip/Bun protein isoforms, namely human TSC22D1.1 (TSC-22 long), human TSC22D2.1, human TSC22D4, and Drosophila BunA and BunF, two short stretches of high conservation but unknown function have been identified (domain 1 and domain 2, Figure 1D, ). Interestingly, two alleles recovered in our screen carry a mutation leading to an amino acid exchange in domain 2, supporting the functional importance of this domain. The other six EMS alleles cause a premature termination of translation.
The short isoforms BunB and BunC are not involved in growth regulation
Our EMS alleles of bun are the first point mutations in the bun locus. Strikingly, all eight mutations exclusively affect the long Bun isoforms, BunA and BunF (Figure 1D). Furthermore, ubiquitous overexpression of bunA, but not of bunB or bunC, was sufficient to rescue the pinhead phenotype and the recessive lethality of bun (Figure 1C, Methods, and data not shown). The fact that neither bunB nor bunC mutations were found in our screen could be explained in two ways. Either bunB and bunC specific exons (as well as the 3' exon common to all transcripts) were not hit by the mutagenesis because they represented considerably smaller targets, or mutations in bunB and bunC did not result in a growth phenotype. In order to assess the growth function of the individual isoforms, we generated isoform-specific deletions presumably resulting in a complete loss-of-function of the respective isoform. We also generated a deletion affecting all isoforms (Figure 1E, Methods). All EMS mutations and deletions affecting both bunA and bunF are subsequently referred to as bunA alleles. Animals homozygous for the bunA deletion alleles (A-211B and A-149B) as well as for the allele affecting all isoforms (200B) died mostly at the larval stage. The lethality of all hetero- and homoallelic combinations was rescued by ubiquitous expression of a bunA transgene (data not shown). Conversely, the homozygous bunB and bunC mutants were viable, fertile, and of normal size. Functional redundancy of BunB and BunC could be excluded because ubiquitous overexpression of bunA was sufficient to rescue the lethality of allele 200B, thus reflecting a bunB and bunC double mutant situation (data not shown).
Allelic series of bunAalleles
We attempted to further characterize the bunA specific growth deficit. The recessive lethal bunA alleles were crossed to a deletion removing the bun locus (Methods; and data not shown) to classify the alleles according to the strength of the hemizygous larval phenotypes. The allele affecting all Bun isoforms (200B) was considered to be null because Bun proteins lacking the TSC-box and the leucine zipper are likely to be non-functional. 200B mutant larvae were massively reduced in body size, reached the third larval instar with a delay of 24 hours, and died within few days after having reached this stage. However, the bunA deletion alleles (A-149B and A-211B) and the bunA EMS alleles leading to a stop codon displayed stronger phenotypes. They developed more slowly and died during the second and third larval instars. In the case of the bunA EMS alleles leading to a stop codon, very few L3 larvae survived up to 14 days (control larvae pupariate after five days) and in rare cases they initiated pupariation but died as pseudo-prepupae. A-R508W and A-P519L displayed much milder phenotypes. These mutant larvae accumulated more mass, most of them developed into L3 larvae, and some into prepupae.
We concluded the following allelic series: strong bunA alleles (bunA deletion alleles > bunA EMS alleles resulting in a premature stop) > 200B > A-R508W, A-P519L. The larval phenotypes of strong bunA deletion and EMS alleles are more severe than those displayed by the deletion allele affecting all bun isoforms, indicating that lacking bunA function alone is more deleterious than lacking all Bun isoforms. Consistently, heteroallelic combinations of strong bunA alleles with 200B resulted in intermediate larval phenotypes. The balance of Bun isoforms may indeed be important because the Bun proteins share the C-terminal putative DNA-binding TSC-box and the leucine zipper for dimerization. Thus, if only bunA is lacking, the short isoforms may form unfavorable dimers, or they may take over the binding to common interaction partners or the regulation of common target genes.
BunA function is required to promote cellular growth
The reduced cell number in clones of bunA mutant cells could be due to a decrease in cellular growth or to an increase in apoptosis. Caspase-3 is one of the key executioners of apoptosis  and it is activated by proteolytic cleavage . Staining for cleaved Caspase-3 in proliferating larval wing discs did not reveal enhanced apoptosis in bunA mutant tissue (data not shown). Furthermore, blocking caspase-mediated apoptosis by the expression of either baculovirus p35  or Drosophila inhibitor of apoptosis 1 (DIAP1)  did not substantially suppress the bunA pinhead phenotype (data not shown). Thus, the bunA growth phenotype is caused by an autonomous reduction in cell size and a reduction in cell number, and apoptosis does not significantly contribute to the reduced proliferation rate.
Flies with reduced bunAfunction are growth-deficient
A quantification of ommatidia number and size in eyes of hypomorphic bunA mutant viable females revealed fewer and smaller ommatidia (Figure 4C). Consistently, a reduction in wing area was detected in females carrying one of the strong bunA alleles, A-149B or A-211B, in combination with either ΔGE12327 (a precise excision allele that we used as control) or GE12327 (data not shown). The small wing phenotype was predominantly caused by a reduced cell number since the cell density was not significantly increased (data not shown). Additionally, we found that the small bun A-149B or A-211B/GE12327 females contained more lipids (total triglycerides) per weight than controls (Figure 4D).
However, the defects observed in hypomorphic bunA mutants were not solely related to growth and metabolism. bunA mutant viable flies, primarily females and combinations of GE12327 with strong bunA alleles, also displayed a rough eye phenotype. Various subtle differentiation defects contributed to the rough eye, including under-rotation of ommatidia (especially around the equator, Figure 4E and 4E'), fusions of ommatidia, and cell fate transformations. With a low frequency, the R4 photoreceptor cell adopted the cell fate of the R3 cell, and a few R7 cells transformed to R1/R6 cells (Figure 4F and 4F'). Eye sections containing large bunA mutant clones (produced with EMS or deletion alleles) revealed the same differentiation defects (data not shown). The cell fate transformation phenotypes are similar to the eye phenotypes associated with low Notch activity [45–48], consistent with a role of bun in Notch signaling .
A sensitized system reveals dominant negative effects of bunB and bunC
Taken together, bunA displayed a gain-of-function growth phenotype in a sensitized system caused by compartment-specific expression of a growth-promoting gene (dS6K) in the developing wing. This sensitized system additionally revealed opposite growth effects of both bunB and bunC. Because lowering the gene dosage of bunA did slightly enhance the bunC overexpression phenotype and because bunA and bunC overexpression neutralize one another, bunC (and possibly bunB) is likely to act on bunA in a dominant negative manner.
Here we show that bunA functions in growth control. BunA positively regulates growth by adjusting cell number and cell size during Drosophila development. Additionally, we found that the short Bun isoforms can act in a dominant negative way on BunA function.
The bun genomic locus gives rise to six different transcripts. Since each transcript has at least one distinct 5' exon, the expression of the six mRNAs is likely to be controlled by separate promoters. The distinct 5' exons result in Bun proteins with individual N-termini, except for BunF that is almost identical to BunA. All Bun isoforms have a common C-terminus comprising a conserved DNA-binding domain (TSC-box) and a leucine zipper for homo- and heterodimerization [27, 51]. It is conceivable that the Bun isoforms exert different functions, since BunA, but not BunB and BunC, is involved in growth control. BunB and BunC might be (partially) redundant to other proteins, for example BunD and BunE, and hence they would only exhibit their mutant phenotypes in double mutant situations. However, our data allow us to conclude that BunA is the major Bun isoform involved in growth control because restoring bunA function suffices to rescue the lethality and the growth deficit associated with a deletion allele that removes the TSC-box and the leucine zipper and thus presumably represents a complete loss-of-function for all Bun isoforms.
Flies with impaired bunA function are small due to fewer and smaller cells. Consistently, clones of cells lacking bunA function remain smaller than their sister clones, and the reduction in clone area is also caused by a diminution of both cell size and cell number. Since apoptosis is not obviously enhanced in clones of bunA mutant cells, we conclude that bunA is required to adjust cellular growth. In line with our results, Wu and colleagues (manuscript submitted) found that BunA exerts similar growth effects in follicle cells and in cultured Drosophila S2 cells.
The bunA growth phenotypes are reminiscent of the phenotypes caused by an impairment of insulin signaling [52–56]. Furthermore, bunA also affects lipid metabolism, as has been shown for insulin signaling [53, 57]. Therefore, we tested whether bunA would genetically interact with insulin signaling components (data not shown). However, we concluded that BunA is probably not a core component of the insulin signal transduction cascade because we did not detect a clear epistatic relationship with the lipid phosphatase PTEN. It is also unlikely that BunA acts directly in the TOR signaling branch because bunA mutant larvae do not display the pronounced growth deficit of the endoreplicative tissues (salivary glands, fat body) that has been observed in dTOR and Rheb mutant larvae [58, 59].
BunA is clearly distinct from insulin signaling components in that it also affects pattern formation. Flies with lowered bunA function display various eye phenotypes reminiscent of defects associated with reduced Notch signaling activity [45–48]. Dobens and colleagues  have proposed a model whereby bun modulates Notch signaling by indirectly adjusting the amount of the Notch ligand Serrate during eggshell development. A similar relationship between bun and Notch signaling may account for the function of bun in patterning processes such as photoreceptor cell differentiation. bun genetically interacts with the EGF receptor and Dpp (BMP-2/-4 ortholog) signaling cascades during eye development  as well as during oogenesis . Presently, it is unclear whether bunA has distinct patterning and growth functions or whether it operates at the interface between pattern formation and growth regulation by integrating various patterning signals to adjust cellular growth.
BunA influences cellular growth and proliferation yet the mechanism remains unknown. In light of the putative transcriptional regulator function of BunA, it is conceivable that bunA induces the expression of growth-promoting genes or it represses the expression of growth inhibitors. However, Treisman and colleagues  have reported that BunA predominantly localizes to the cytoplasm in the larval eye disc. In addition, we could not detect any nuclear signal upon expression of an N- or C-terminally GFP-tagged BunA in Drosophila S2 or Kc cells (data not shown). Thus, BunA might shuttle between the cytoplasm and the nucleus, and its translocation to the nucleus might be tightly regulated. Alternatively, BunA could function in the cytoplasm in a process distinct from transcriptional regulation. The identification of BunA binding partners should shed light on the subcellular environment in which BunA exerts its function.
Our study on the growth-promoting function of bunA in Drosophila may influence the perspective on the mammalian homologs of Bun, especially on TSC-22 (TSC22D1). Whereas the longer isoform of TSC-22 (TSC22D1.1) is similar to BunA (and BunF), the shorter isoform (TSC22D1.2) resembles BunB, BunD, and BunE. Data from numerous studies suggest that TSC22D1.2 acts as a tumor suppressor [4–7, 10–12], which is at odds with the fact that only bunA is involved in growth regulation in Drosophila, and that BunA behaves rather opposite to a tumor suppressor. The results from our in vivo analysis may be of special interest in this context, since the relative balance of bun transcripts is important (allelic series) and overexpression of bunC (and also bunB) interferes with bunA function in a dominant negative manner. If this interaction is conserved in mammals, we can envision the following scenario for how the TSC-22 locus may be involved in tumor suppression. Whereas the long TSC-22 isoform, TSC22D1.1, positively regulates cellular growth (as does BunA), the short isoform, TSC22D1.2, inhibits growth by competing with TSC22D1.1. The antagonism between the long and the short isoforms can be achieved at several levels. An excess of the short isoform could lead to the formation of non-functional heterodimers, or the two isoforms could compete for another dimerization partner. Provided that TSC-22 functions in transcriptional regulation, the two isoforms might also contribute to differential regulation of target genes. In either case, the long TSC-22 isoform could be hyperactivated as a consequence of the loss of the short isoform. Thus, the short isoform could act as a tumor suppressor by keeping the long isoform in check. Our findings should encourage further studies in mammals that distinguish between the TSC-22 isoforms and that primarily focus on the function of the long TSC-22 protein, TSC22D1.1.
In an unbiased screen for growth-regulating genes in Drosophila, we have isolated mutations in bunched, the only Drosophila locus that encodes proteins homologous to the mammalian TSC-22 family proteins. Our genetic analysis of bun revealed BunA as a positive growth regulator that adjusts cellular growth and proliferation. The short isoforms BunB and BunC are not required for normal growth, but they can interfere with BunA function in a dominant negative manner. This is the first report on the different in vivo functions of long and short isoforms of TSC-22 family members. In light of our findings, the analysis of the tumor suppressor function of mammalian TSC-22 requires a rigorous distinction of the long and short isoform. We propose that the long TSC-22 protein (TSC22D1.1) is a functional homolog of BunA in growth regulation, and that its function is antagonized by the short TSC-22 protein (TSC22D1.2). Thus, loss of TSC22D1.2 may result in deregulated TSC22D1.1 activity.
Breeding conditions and fly stocks
Flies were kept at 25°C on food described in . For the genetic mosaic screen y, w, eyFLP; FRT40A, w+, cl2L3/CyO, y+  flies were used. Clonal analyses in the adult eyes and imaginal wing discs were carried out with y, w, hsFLP; FRT40A, w+  and y, w, hsFLP; FRT40, Ubi-GFP (Bloomington Drosophila Stock Center, modified) flies, respectively. Complementation tests were performed with bun alleles 00255 (Bloomington Drosophila Stock Center; described in ), 04230, 06903, and rI043 . The bunA pinhead phenotype was rescued by driving UAS-bunA  with ey-Gal4 (insertion on 3rd chromosome; U. Walldorf, Medizinische Fakultät, Universität des Saarlandes, Homburg, D) recombined with GMR-Gal4 (insertion on 3rd chromosome, unpublished). In the four independent jump-out screens the EP-elements GE12327, GE14917, GE11969, and GE12921 (GenExel Inc., commercially available) were mobilized using a Δ2–3 transposase strain (; Bloomington Drosophila Stock Center). The resulting deletion alleles were recombined onto FRT40A chromosomes . For allelic series Df(2L)Exel6033 was used (Bloomington Drosophila Stock Center). For the overexpression studies in the adult wing the following fly strains were used: ap-Gal4 (described in ); ap-Gal4, UAS-dS6K ; UAS-bunB , and UAS-bunC (XW and LR, manuscript submitted).
eyFLP/FRT screen, mapping of EMS mutations, and rescue experiments
The eyFLP/FRT technique  was used to produce mosaic flies with eyes and head capsules largely homozygous for a randomly induced mutation. The rest of the body (including the germ line) remained heterozygous and was therefore phenotypically wild-type (screen described in ).
The eight EMS alleles of a complementation group on 2L were mapped using visible markers and large deletions (Df(2L)prd1.7 and Df(2L)Prl failed to complement the EMS alleles; Bloomington Drosophila Stock Center) to the cytological interval 33B2-F2. Mapping data obtained with molecular markers (P-elements and SNPs, details available upon request) further narrowed down the candidate region to 33E7-F2 and pointed to the distal border of the candidate region where 5' exons of bun were located.
Using the UAS/Gal4 system , we tested whether ubiquitous overexpression at different levels – achieved by armadillo-Gal4, daughterless-Gal4, and actin5C-Gal4 – of bunA, bunB or bunC transgenes would rescue the lethality of bunA alleles. Although the bunB and bunC transgenes resulted in strong protein expression (as assessed by Western blots on larval lysates), they could not rescue the lethality associated with bunA mutations.
Jump-out screens and allelic series
The GenExel EP-element insertions were isogenized (y, w; GE iso [w+]/CyO) prior to mobilization achieved by crossing to Δ2–3 flies (y, w; Sp/CyO; Δ2–3, Sb/TM6B). F2 males lacking the mini-white eye marker were collected after mating, and DNA of 10 flies was pooled and amplified by PCR using primers flanking the regions of interest (primer sequences available upon request). Deletions were identified by gel electrophoresis and analyzed by sequencing. Positive pools were split up to single flies to identify the individuals carrying the deletions. Deletions A-149B and A-211B, both beginning 343 bp upstream of the bunA start codon, removed 2513 bp and 2038 bp of genomic DNA, respectively, including regions coding for domain 1 and 2. In alleles B-132A and B-181A, the deletions extended from 217 bp upstream to 126 bp and 20 bp downstream of the bunB start codon, respectively. The deletion C-158B started 341 bp upstream of the bunC start codon and eliminated the whole ORF of the first bunC exon (613 bp in total). 200B removed the entire coding region and the splice acceptor site of the common bun exon (starting 29 bp upstream of the common bun exon and extending for 641 bp).
Allelic series was determined by crossing bun alleles (y, w; bun-/CyO, y+) to a deficiency removing the bun locus (y, w; Df(2L)Exel6033/CyO, y+). Animals were reared on agar plates supplemented with yeast at 25°C.
Clones in the adult eyes were induced 24–48 hours AED by a heat shock for 1 hour at 34°C in animals of the genotype y, w, hsFLP/y, w; FRT40A, w+/FRT40A, bun-. For tangential eye sections adult fly heads were cut in half using a razor blade and shortly stored in Ringers on ice. Eyes were then fixed as described in . For the clonal analysis in the larval wing discs, y, w, hsFLP/y, w; FRT40A, Ubi-GFP/FRT40A, bun- animals were given a heat shock for 25 minutes at 34°C 24–48 hours AED. Larvae were dissected in Ringers 51–52 hours after the heat shock, and the discs were fixed in 4% paraformaldehyde (in 1 × PBS) for at least 1 hour on ice. Nuclei were stained by incubation for 30 minutes in DAPI (0.5 μg/ml in 1 × PBS) at room temperature, and wing discs were mounted in Vectashield Mounting Medium. Pictures were taken using a Leica SP2 confocal laser scanning microscope.
For the quantification of the clones, ommatidia in mosaic eyes and cell number in larval wing discs were counted, and the clone area in larval wing discs was determined using Adobe Photoshop 7.0. In tangential eye sections, the area enclosed by rhabdomeres from photoreceptor cells R1–R6 was measured in mutant ommatidia (lacking pigmentation) and in neighboring wild-type sized ommatidia (pigmented). Student's t-tests were used to test for significance.
Analysis of adult flies
Adult flies reduced in BunA function: Freshly eclosed males and females of the genotype y, w; bunGE12327/FRT40A, bunA-149B or A-211Bor y, w; bunGE12327/ΔGE12327 were kept together on fresh food for two days. For weight experiments the flies were exposed to 95°C for 5 minutes and air-dried at room temperature for 3 days. The dry weight of individual flies was assessed using a Mettler Toledo MX5 microbalance. For the analysis of adult eyes and lipid contents the flies were frozen at -20°C. Single ommatidia were counted on scanning electron micrographs, and the areas of seven adjacent ommatidia in the center of the compound eye were measured using Adobe Photoshop 7.0. Lipid levels were quantified as described in .
Overexpression of Bun isoforms using the UAS/Gal4 system : Several ubiquitous and wing-, eye-, and fat body-specific Gal4 driver lines – namely armadillo-Gal4, actin5C-Gal4, daughterless-Gal4, GMR-Gal4, ey-Gal4, MS1096-Gal4, C10-Gal4, ap-Gal4, and pumpless-Gal4 – were tested with GE12327, UAS-bunA, UAS-bunB, and UAS-bunC. Neither single nor combined overexpression of the constructs led to altered growth. The combinations of actin-Gal4 with UAS-bunA or GE12327 were lethal. GE12327 led to expression of BunA but not of the short Bun isoforms (as assessed by Western blots on larval lysates).
Genotypes of adult flies with wing phenotypes: y, w; ap-Gal4/GE12327; y, w; ap-Gal4, UAS-dS6K/UAS-eGFP; y, w; ap-Gal4, UAS-dS6K/GE12327; y, w; ap-Gal4, UAS-dS6K/+; UAS-bunA, UAS-bunB or UAS-bunC/+; y, w; ap-Gal4, UAS-dS6K/bunA-211B; UAS-bunC/+; y, w; ap-Gal4, UAS-dS6K/+; UAS-bunA, UAS-bunC/+.
We thank G. Dietzl, B. Dickson, J. Treisman, U. Walldorf, and the Bloomington Drosophila Stock Center for fly strains; C. Köpfli and M. Jünger for reagents; C. Hugentobler, A. Baer, B. Brühlmann, A. Strässle, and R. Grunder for technical assistance, and P. Gallant and K. Basler for helpful suggestions. This work was supported by the Swiss National Science Foundation and the Kanton of Zürich.
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