Differential requirement for Dab2 in the development of embryonic and extra-embryonic tissues
© Moore et al.; licensee BioMed Central Ltd. 2013
Received: 13 May 2013
Accepted: 25 October 2013
Published: 29 October 2013
Disabled-2 (Dab2) is an endocytic adaptor protein involved in clathrin-mediated endocytosis and cargo trafficking. Since its expression is lost in several cancer types, Dab2 has been suggested to be a tumor suppressor. In vitro studies indicate that Dab2 establishes epithelial cell polarity and organization by directing endocytic trafficking of membrane glycoproteins. Dab2 also modulates cellular signaling pathways by mediating the endocytosis and recycling of surface receptors and associated signaling components. Previously, two independent gene knockout studies have been reported, with some discrepancies in the observed embryonic phenotypes. To further clarify the in vivo roles of Dab2 in development and physiology, we designed a new floxed allele to delete dab2 gene.
The constitutive dab2 deleted embryos showed a spectrum in the degree of endoderm disorganization in E5.5 and no mutant embryos persisted at E9.5. However, the mice were grossly normal when dab2 deletion was restricted to the embryo proper and the gene was retained in extraembryonic tissues using Meox2-Cre and Sox2-Cre. Adult Dab2-deficient mice had a small but statistically significant increase in serum cholesterol levels.
The study of the new dab2 mutant allele in embryos and embryoid bodies confirms a role for Dab2 in extraembryonic endoderm development and epithelial organization. Experimental results with embryoid bodies suggest that additional endocytic adaptors such as Arh and Numb could partially compensate for Dab2 loss. Conditional deletion indicates that Dab2 is dispensable for organ development, when the vast majority of the embryonic cells are dab2 null. However, Dab2 has a physiological role in the endocytosis of lipoproteins and cholesterol metabolism.
Mouse Disabled-2 (Dab2) was isolated as a 96 kDa phosphoprotein involved in CSF-1 signaling in macrophages, and was initially referred to as p96 . A fragment of the Dab2 human cDNA was also isolated based on its frequent loss of expression in ovarian cancer, and was termed DOC-2 (Differentially expressed in ovarian carcinoma gene 2) . Sequence homology suggests that the p96 protein is one of the two mammalian orthologs of the Drosophila Disabled gene ; hence, that was the origin of the naming for the neuronal expressed mammalian Dab1  and the more ubiquitously expressed Dab2 [1, 5].
The loss of expression of Dab2 in ovarian cancer and growth regulatory properties in cell culture studies led to the suggestion that Dab2 is a tumor suppressor in ovarian cancer [6, 7]. Subsequently, loss or reduction of Dab2 expression was found in other cancer types including rat mammary tumors , breast cancer [9, 10], colon cancer , esophageal cancer , urothelial carcinomas , prostate cancer , head and neck cancer , and nasopharyngeal carcinomas . Mechanisms were also suggested for Dab2 in epithelial organization [10, 17, 18], and in the regulation of Ras/MAPK [19–22], TGF beta [15, 23, 24], and Wnt [25–28] signaling pathways.
Cell biology studies revealed that Dab2 is an endocytic adaptor protein . Dab2 contains an N-terminal PTB domain that binds cell surface proteins with an NPXY motif in their cytoplasmic tails ; several motifs that bind clathrin and adaptin proteins ; and a C-terminal region that binds myosin VI, a directional motor protein [32, 33]. Thus, Dab2 mediates the simultaneous attachment of clathrin-coated cargos containing transmembrane proteins with one or more NPXY motifs, such as the low density lipoprotein (LDL) receptor, megalin, and integrins, to the myosin motor, enabling endocytosis and directional trafficking. A role of Dab2 in endocytosis and trafficking of integrins and thus cell mobility has also been suggested [34, 35]. Modulation of LDL receptor endocytosis by Dab2 has also been studied in cultured cells , though a role in vivo has not yet been established. The polarized trafficking of cell adhesion molecules such as integrins and E-cadherin may explain the role of Dab2 in epithelial polarity and organization  and trafficking of signaling surface receptors may account for its activity in modulating multiple signaling pathways [5, 28, 37].
To determine if any of these cellular mechanisms may be biologically relevant and significant, gene deletions in mice have been performed [17, 38]. A gene replacement of dab2 allele by betaGal-Neo led to the finding of early embryonic lethality in the knockout mice . In the mutants, extraembryonic endoderm cells intermixed with ectodermal cells in the E5.5 embryos, and a visceral endoderm layer failed to develop [17, 18]. The aged heterozygous mice were found to develop ovarian cysts and preneoplastic lesions in both ovaries and uteri . A flox dab2 mutation was made and the homozygous deleted mutant mice were also embryonic lethal, but the mutant embryos were found to persist to a later stage . Additionally, a mosaic dab2 deletion using a Meox2-Cre line was found to produce remarkably normal mice with minor defects in kidney function .
One possibility to explain the discrepancy between these two dab2 mutant lines is that the expression of betaGal-Neo in the dab2 mutant embryos  might lead a more severe phenotype. Another idea is that the dab2 flox mutant allele  may produce truncated proteins from an alternative translation start site. Hence, the later knockouts may not be complete nulls and the mutant embryos may be able to persist longer.
To investigate the differences in the two knockouts and to study further the relevant biological functions and possible tumor suppressor role of Dab2, we have designed another flox dab2 mutant allele with a more extensive exon deletion, produced the constitutive and conditional mutant mice, and examined the phenotypes. Here, we report a detailed characterization of the embryonic phenotypes, the conditional knockout mice, the mutant embryonic stem cells, and the embryoid bodies.
Construction of floxed dab2 mutant allele and mice
Upon transfection of ES cells with the linearized targeting construct and G418 selection, 380 independent drug-resistant clones were picked and screened for homologous recombination by PCR (Figure 1B). A total of 6 clones were identified as potentially homologous targeted lines, and 2 of the clones were characterized by PCR as containing the complete targeting construct and correct homologous replacement at both 5’ and 3’ ends (Figure 1C). These two lines of ES cells were used for blastocyst injections, chimeric mouse production, germ-line transmission, and establishment of the mutant colonies. The Neo locus was then removed by crossing with FLPeR mice [40, 41] (Figure 1A). The two lines of the conditional mutant mice were bred to homozygous dab2 flox, and the transmission of dab2 (+/+), (+/fl), and (fl/fl) followed a Mendelian ratio (Additional file 1: Table S1 and Table S2). Homozygous flox mice exhibited wildtype characteristics with normal Dab2 expression, reproductive capability, and lifespan. We conclude that the flox alleles do not influence dab2 gene activity and the flox mice are essentially wildtype, and the two dab2 mutant lines are identical.
Characterization of Dab2 constitutive null mutant embryos
The dab2 flox mice were crossed with Meox2-Cre transgenics [42, 43] to delete the dab2 gene to generate delta flox (df) (Figure 1A). Here, we refer to the dab2-deleted allele in mosaic mice as “df” and constitutive null in germ-line deletion as “-”. Next, we selected both male and female progenies with the dab2 (+/-) genotype for further matings to investigate the embryonic phenotype of dab2 constitutive knockouts, referred to as dab2 (-/-).
By E5.5, the disorganization of the extraembryonic tissues derived from the primitive endoderm was apparent (Figure 2B). For comparison, all the Dab2-positive E5.5 embryos, of either dab2 (+/+) or (+/-), were well structured with a Gata4- and Dab2-positive ring of visceral endoderm surrounding the epiblast (Figure 2B, WT). A thin layer of parietal endoderm was present at the outer layer (Figure 2B, WT, only one Gata4-positive nucleus is visible in this section, indicated by an arrow). Of the 65 E5.5 embryos sectioned and analyzed, 55 were Dab2-positive (either wildtype or heterozygous), and 10 were confirmed as null. In the Dab2-negative E5.5 embryos, Gata4-positive endoderm cells were misplaced and intermixed within the egg cylinder core in all mutant embryos analyzed (Figure 2B), as shown in the 5 representative examples of sections through the center of the embryos. Unlike wildtype embryos that had uniformly well patterned endoderm epithelium, all mutant embryos showed a spectrum in the degree of endoderm disorganization ranging from mild to severe (Figure 2B, mutant embryos #1-5).
Genotyping of dab2 constitutive mutant embryos recovered at E9.5
Characterization of dab2 conditional mutant mice
Subsequently, we used another Cre line, the Sox2-Cre transgenic , in an attempt to delete dab2 to a greater extent. Like Meox2, Sox2 is expressed in the epiblast but not in extraembryonic tissues, and Sox2-Cre line can efficiently delete the floxed gene in the embryo proper [42, 43]. The conditional mutants were generated by crossing female dab2 (fl/fl) with male dab2 (df/+);Sox2-Cre mice. Here, male rather than female carriers of Sox2-Cre were used to avoid a maternal effect, which, due to Cre expression in oocytes, causes non-Mendelian transmission [42, 43]. We found that the dab2 “fl” allele was consistently undetectable by PCR in tails of conditional knockouts at 3 weeks of age (Figure 3D). Comparisons of representative PCR amplifications from heterozygous, conditional knockouts using Sox2-Cre or Meox2-Cre, respectively, are shown (Figure E). The kidney from adult Sox2-Cre conditional knockouts lacked any Dab2-positive cells (Figure 3F-H). Nevertheless, dab2 (df/fl);Sox2-Cre mice were produced at a Mendelian ratio and were devoid of any obvious developmental defects. Both male and female mutant mice were fertile and had normal breeding ability when paired with a wildtype partner (Additional file 1: Table S1 and Table S2).
Up to now, we have characterized more than 200 progenies with a dab2-deleted genotype and have observed no obvious developmental phenotype. However, the aged mice exhibited an increased incidence of preneoplastic lesions, mainly in the uterus, ovary, mammary gland, and colon, similar to the previous report for the dab2 heterozygous mice . The studies of the tumor phenotypes of aged Dab2 knockout mice and in combination with p53 mutation will be reported elsewhere.
Role of Dab2 in cholesterol metabolism in vivo
We made additional attempts to determine possible physiological phenotypes of the Dab2-deficient mice based on known cellular functions of the Dab2 protein. Dab2 is an endocytic adaptor protein for several NPXY motif-containing cell surface receptors, including lipoprotein (LDL) receptor, megalin, and integrins. Endocytosis of megalin mediated by Dab2 is thought to play a role in protein re-uptake in the proximal tubule cells of kidney, and previously it was found that excessive proteins were present in the urine from Dab2-deficient mice . A mild proteinuria phenotype was also observed in the Dab2-deficient (dab2 (df/fl);Sox2-Cre) mice in our current study.
In our breeding scheme of using male dab2 (+/df);Sox2-Cre and female dab2 (fl/fl) parents, no dab2 wildtype littermates were generated to be used as controls. However, the serum lipid profiles of dab2 (fl/fl) mice were found identical to those of heterozygous and containing Cre transgene (dab2 (+/df); Sox2-Cre), indicating there was no dosage effect of dab2 gene nor an impact of Cre expression on cholesterol metabolism.
Thus, the results support the findings from cell culture studies [36, 45] that Dab2 is involved in LDL receptor endocytosis and LDL uptake, and suggest that Dab2 has a physiological role in cholesterol metabolism.
Analysis of dab2-null embryoid bodies and embryos for adaptor proteins
We further determined if compensatory expression of Arh and Numb adaptor proteins occurred in dab2 knockout embryos. E9.5 embryos from matings between female dab2 (fl/fl) and male dab2 (+/df);Sox2-Cre were harvested, and the entire embryo propers dissected free of extraembryonic tissues were analyzed by Western blotting (Figure 7C). Although some variation in levels of Numb and Arh expression were visible in dab2 heterozygous E9.5 embryos, global expression of Numb and Arh proteins was consistently elevated in the four dab2-deleted embryos analyzed (Figure 7C), suggesting there was compensatory expression of Arh and Numb in the absence of Dab2 during embryonic development. Dab1, the other Drosophila Disabled ortholog, was undetectable in ES cells or E9.5 embryos, either wildtype or mutant. Involved in neuronal migration and with a brain-restricted expression , Dab1 likely has little function or expression overlap with Dab2.
The current dab2-conditional deletion mouse model has verified the critical role of Dab2 in the organization of the primitive endoderm [17, 18]. However, normal development following its deletion in the embryo proper indicates that the previously suggested roles of Dab2 in the regulation of signaling pathways in unchallenged physiological situations are either very subtle or redundant in vivo.
Mechanism of epithelial organization and the embryonic lethal phenotype of dab2 knockout mice
The current study confirms the previous observations that Dab2 deficiency leads to disorganization of the extraembryonic endoderm [17, 18]. Moreover, we observed primitive endoderm disorganization at E4.5, a stage that immediately follows blastocyst implantation. The extraembryonic endoderm cells are present in Dab2-deficient embryos, but intermingled with the epiblast cells instead of forming a surface epithelial layer. This phenotype of Dab2-deficient embryos prompts us to describe dab2 as an “epithelial surface positioning gene” , in parallel to the manner in which dab1 is referred to as a “neuronal positioning gene” . The proposed mechanism is that Dab2 mediates directional endocytic transport in the endoderm epithelial cells, leading to the establishment of an apical polarity [18, 46]. Previously, it was considered that the highly adhesive embryonic cells sort to inside and less adhesive cells sort to the periphery , according to the Differential Adhesion Hypothesis [48, 49]. However, in the case of the mouse primitive endoderm, the ability to establish apical polarity rather than differential adhesive affinity determines the anchoring of the primitive endoderm layer at the surface . Thus, deletion of dab2 leads to the loss or reduced trafficking of cell surface adhesion molecules, consequently a reduced ability to establish a polarity, and finally the failure of primitive endoderm cells to position at the surface [18, 46].
Additionally, deletion of c-fos can partially rescue endoderm disorganization in the Dab2-deficient embryos . Dab2 can negatively modulate c-Fos expression , and c-Fos can regulate epithelial polarity . Thus, Dab2 may also impact endoderm cell organization through its influence on the MAPK/c-Fos signaling pathway. Although, it was reasoned previously that additional activities of Dab2 other than suppression c-For might impact organogenesis , the current result indicates Dab2 developmental requirement is restricted to extraembrynic endoderm. However, it is still possible that Dab2 expression within extraembryonic endoderm may influence embryonic development by a non-cell autonomous mechanism.
Lack of developmental phenotypes in dab2 mosaic mutant mice
Considering that the loss of Dab2 in primitive endoderm leads to the disruption of epithelial organization and a severe phenotype in early embryos, we were perplexed that dab2 conditional knockouts generated using either Meox2-Cre or Sox2-Cre showed no developmental defects and had relatively mild adult phenotypes. One possibility is that remnant Dab2-positive cells in the mosaic dab2 mutant mice are sufficient for essential developmental functions. Alternatively, expression of endocytic adaptor proteins such as Numb and Arh, which have similar structures and functions as Dab2 , may compensate when dab2 is deleted. Based on PCR genotyping of tail tissues and immunostaining of kidney, we estimated that around 5-10% of the cells still contain undeleted dab2 gene in Meox2-Cre conditional knockout. Conversely, the “fl” allele was undetectable in Sox2-Cre knockout lines, yet the percentage of Dab2-positive cells could be higher in the earlier stages of embryonic development. Sox2 is expressed in and essential for the trophoblast extraembryonic lineage [51, 52]. However, Sox2-Cre-mediated deletion of dab2 gene did not affect trophectoderm development, thus Dab2 is also not essential in at least a subset of the trophoblast lineage. Although the dab2 gene can be deleted in the majority of somatic cells without affecting embryonic development, we considered the possibility that a miniscule fraction of Dab2-positive cells may be sufficient to fulfill its potentially critical roles. Nevertheless, we did not detect any remaining dab2 allele in E9.5 Sox2-Cre conditional knockout embryos. Most likely, compensatory expression of Dab2-like proteins such as Arh and Numb accounted for a mild phenotype and the actual formation of primitive endoderm structures observed in some of the Dab2-deficient embryoid bodies.
Dab2 mediates endocytosis and contributes to lipoprotein metabolism in vivo
Although Dab2-deficient mice were overtly normal, mutant mice did have several physiological differences, such as excessive excretion of plasma proteins in urine and increased serum cholesterol and LDL. These physiological phenotypes might be attributable to the adaptor activity of Dab2 for megalin and LDL receptor, respectively. Another well-documented Dab2 binding ligand is integrin [34, 35]. Though an impact of Dab2 on cell migration was observed in cultured cells, we found no related physiological phenotype in the mutant mice. Dab2 also has been reported to have many other activities, but these phenotypes were also not readily observable in the mutant mice.
In the case of LDL receptor endocytosis, the increased cholesterol and low density lipoprotein levels in Dab2 deficient mice was small but readily detectable. The increased serum cholesterol and LDL levels was likely due to the absence of Dab2 protein in mediating uptake of LDL particles from circulation by Dab2-mediated endocytosis. The results support the findings in cell culture studies [36, 45] that Dab2 is involved in LDL receptor endocytosis and LDL uptake, and suggest that Dab2 has a physiological role in cholesterol metabolism. Normally, mice have a relatively low level of serum LDL. Possibly, the difference in serum cholesterol and LDL levels would be more significant in Dab2-deficient mice when the animals are placed on a high fat diet. The impact of Dab2 on cholesterol metabolism, the cell types affected, and comparison with LDL receptor and Arh mutant mice, will be interesting topics of future investigations.
Likely, as an endocytic adaptor for several cell surface receptors, Dab2 may influence the endocytosis and secretion of multiple ligands, and may regulate multiple signaling pathways. The function of Dab2 is pleiotropic, although it may serve as a fine tune regulator of signaling and an endocytic adaptor with overlapping specificity with other adaptors, but the impact of its deletion is mild when observed in normal physiological setting. However, additional phenotypes and Dab2 functions may be observed in deeper analysis of the Dab2 deficient mice, and also when placing the mutant mice in a challenged situation.
Cellular actions of Dab2 in signaling and cell adhesion
Dab2 has been implicated in regulating signaling pathways, including the Ras/MAPK/c-Fos [19–22], the TGF-beta [15, 23, 24], and the Wnt pathways [25–28]. Dab2 also participates in the endocytic recycling of E-cadherin  and integrins  and thereby influences cell adhesion and mobility. It is plausible that Dab2 affects these diverse cellular signaling pathways due to its role in endocytosis and trafficking; however, whether these activities are physiologically significant has not been resolved previously. The Ras/MAPK pathway [53–59], the TGF-beta pathway , the Wnt pathway [61, 62], E-cadherin [63, 64], and integrins  are all critical in embryonic development and their perturbations impair the process. Since Dab2 deletion in the embryo proper minimally impacts embryonic development, we would argue that the majority of the proposed Dab2 functions generated from these in vitro studies have little relevance in vivo. Dab2 may fine-tune the activities of these signaling pathways, but the effects are subtle and not readily observable in the normal physiology of whole animals.
Loss of Dab2 in epithelial disorganization and cancer
One common observation in both early embryonic development and carcinogenesis is that loss of Dab2 leads to epithelial morphological transformation. The loss of Dab2 correlates well with morphological changes, specifically the transition from a simple epithelial monolayer to a multiple layered neoplasm in ovarian cancer . This is remarkably reminiscent of the disorganization and loss of surface position of primitive endoderm in dab2-deficient embryos and embryoid bodies. Dab2-mediated directional endocytic trafficking leads to the generation of an apical-basal epithelial polarity, and polarity plays critical role in surface positioning . Thus, loss of Dab2 results in epithelial depolarization and subsequent disorganization, as found in ovarian carcinomas and early embryos [17, 18, 39, 66]. However, ovarian surface epithelia are monolayered and intact in dab2-deficient mice (not shown), indicating the loss of Dab2 alone is insufficient to induce epithelial disorganization. Unlike the primitive endoderm epithelium in rapidly growing embryos, mature epithelia in adults most likely possess additional mechanisms to ensure stable architecture.
The current results in the study of a new dab2 conditional mutant allele are consistent with the prior report that dab2 deletion leads to an early embryonic lethality at E5.5 [17, 18]. This lethality occurs much earlier than the other report of mutants derived from a previously reported conditional allele . Consistent with our prior conclusion [18, 19], the current analysis of both embryos and embryoid bodies indicates that loss of Dab2 leads to disorganization of primitive endoderm and subsequent extraembryonic endoderm, although the differentiation to endoderm lineage is not impaired. Thus, the current study clarifies the discrepancy in embryonic phenotypes described for the two previous knockouts [17, 38].
Conditional deletion indicated that Dab2 is dispensable for organ development, when the vast majority of the embryonic cells are dab2 null. A possibility is that additional endocytic adaptors such as Arh and Numb partially compensate for Dab2 loss. However, Dab2 has a physiological role in the endocytosis of lipoproteins and cholesterol metabolism.
Construction of the dab2 homologous recombination targeting vector and conditional mutant mice
Using the mouse Dab2 cDNA as a probe, three clones (λ20, λ24, and λ28) of mouse genomic DNA containing the dab2 gene were isolated from a 129/Sv genome library in λZap (Stratagene) . The λ24 fragment, spanning exons 2 to 11 of the dab2, was cloned at the Eco RI site of pBSKS, and was used as a template for PCR amplifications. A 5.8 kb genomic fragment covering exons 5 to 9 was PCR-amplified with a Not I-anchored sense primer (5′-ATG GAT GCG GCC GCT CCC GGA AAT GGT TAC-3′) and antisense primer (5′-ATG ATG GAT CTT TGG TTG TTG T-3′). The resulting PCR fragment was recloned into Apa I/Not I restricted pK11/pM30 Frt-PGKNeo-Frt-LoxP-pBSSK plasmid (from Dr. David W. Martin, Emory University) which was first modified by cloning a polylinker (Apa I-Sac I-Not I-Stu I-Kpn I) at Apa I/Kpn I sites with the sequence of 5′-CAT GAG CTC AGG CGG CCG CAT AGG CCT AAG GTA C-3′. A 1.7 kb genomic fragment containing exon 2 was PCR-amplified with Cla I-anchored sense primer (5′-ATC GAT CTG CAG TGA GGA TCC TGA ATA CTA TCT CTC GGT ACT-3′) and Sal I-Sac I-anchored antisense primer (5′-ATC GAT GTC GAC TGA GAG CTC CAC ATT CTG CTA ATA TGT CAT C-3′) and inserted into Cla I/Sal I restricted BstLox2 vector (also provided by Dr. D.W. Martin) upstream of the LoxP site near the T7 RNA promoter. A 2.8 kb fragment containing exons 3 and 4 was PCR-amplified with Sac II-anchored sense primer (5′-ATC GAT CCG CGG GAA TGA ATC CTA CCA TGG-3′) and Eco RV-anchored antisense primer (5′-ATC GTA GAT ATC AGC CTG CCA GAG CTG GAG-3′) and recloned into Eco RV/Sac II restricted BstLox2 vector downstream of the LoxP site. The subcloned fragment of 4.4 kb containing exon 2-Loxp-exon 3 and 4 of the dab2 gene was then digested with Sac I and Sac II and recloned into pK11/pM30 Frt-PGKNeo-Frt-LoxP-exons 5 to 9 to generate the final targeting construct. Each PCR fragment generated was amplified using cloned-Pfu DNA polymerase (Stratagene), which has a proofreading activity that results in high-fidelity DNA replication. At each cloning step, several clones were verified by restriction analysis and sequencing. The targeting vector was linearized with Sac I and Avr II to obtain an 11.7 kb fragment that was electroporated into RW4 ES cells.
The selection, screening, and blastocyst injection to produce chimeric mice were performed with assistance from the institutional transgenic mouse facility according to standard procedures. Neomycin resistant ES cell clones were screened for homologous recombination by PCR using discriminating primers spanning sequences both inside and outside the targeting construct. The sequences of the primers are listed: P5′: 5′-CCA GTA CAC CAC GTA AGA AAG-3′; P3′ for WT: 5′-ACA GTC ACT GAT ACC AGC CAA-3′; P3′ for KO: 5-AGT TAT TAG GTC CCT CGA CCT-3′. The fragments from PCR amplification were predicted to be 886 bp for wildtype (WT) and 1714 bp for the recombinant allele. The positive clones were further characterized by PCR to ensure the allele contained all the component of the targeting construct: LoxP 1, 1714 bp (P5′: 5′-CCA GTA CAC CAC GTA AGA AAG-3′; P3′: 5-AGT TAT TAG GTC CCT CGA CCT-3′); LoxP 2, 795 bp (P5′: 5′-ACA TTA TAC GAA GTT ATT CGA GG-3′; P3′: 5′-ATC ACA GTT GGC GTC ATA ACA A-3′); Frt1, 635 bp (P5′: 5′-CAC CTG ATC TGA CTG TGG TT-3′; P3′: 5′-AGA GAA TAG GAA CTT CGG CC-3′); Frt2, 837 bp (P5′: 5′-ACT ATA GGA ACT TCG TCG ACC-3′; P3′: 5′-ATC ACA GTT GGC GTC ATA ACA A-3′); Neo, 617 bp (P5′: 5′-GGC ATT CTC GCA CGC TTC AA-3′; P3′: 5′-CTT GAG CCT GGC GAA CAG TT-3′).
Two selected recombinant ES clones, #28 and #121, were used for blastocyst injection to establish mutant lines. The neo locus flanked by Frt sites was then excised by crossing with FLPeR mice [40, 41]. Two lines of dab2 flox conditional knockout mice (lines 239 and 270) were established from the two independent ES clones and the mouse colonies were maintained in the C57BL/6 J background. The two lines gave identical phenotypes and were not distinguished here unless specifically mentioned.
Mouse strains and husbandry
Flp expression mice (129S4/SvJaeSor-Gt(ROSA)26Sortm1(FLP1)Dym/J) [40, 41], Meox2-Cre mice (B6.129S4-Meox2tm1(cre)Sor/J) , and Sox2-Cre mice (Tg(Sox2-cre)#Amc/J) [42, 68] were purchased from Jackson Labs.
Control and mutant mice were kept in the institutional mouse facility as inbred colonies by pairing parents of selected genotypes. Upon weaning, the mice were genotyped and separated into experimental and control groups accordingly. The mice were caged in barricaded viral pathogen-free rooms under a 12-hour light–dark cycle, with free access to food and autoclaved water. For dietary studies, the mice were kept on a 5 K20 chow (LabDiet: 17% protein, 10% fat, 2.5% fiber, 8% ash, and 3.5% added minerals). All procedures for experiments using animals were reviewed and approved by the University of Miami Miller School of Medicine Institutional Animal Care and Use Committee and followed National Institutes of Health guidelines.
Measurement of serum lipids
Mice were fasted for 6 hours and then anesthetized before blood was collected by cardiac puncture. Blood samples were allowed to clot for 15 min and centrifuged for 10 min in a bench top centrifuge. The serum supernatants were collected and stored at -80°C until use. Lipid analysis was performed by the University of Miami Comparative Pathology Laboratory using a Vitros 250 chemistry analyzer (manufacturer: Johnson & Johnson). Cholesterol, triglycerides, and high-density lipoprotein (HDL) levels were measured colorimetrically. Low-density lipoproteins (LDL) and very low-density lipoprotein (VLDL) levels were derived from the following formula: VLDL = 1/5 of triglycerides; LDL = total cholesterol minus HDL and VLDL).
Derivation of dab2 null ES cells and the formation of embryoid bodies
Blastocysts were harvested at E3.5 after timed-matings between dab2 (+/-) parents. The blastocysts were individually cultured in single wells of 24-well tissue culture plates previously coated with irradiated murine embryonic fibroblasts (MEF). The blastocysts were allowed to hatch and attach to the fibroblast feeder layer. Cells from the outgrowth of blastocysts were expanded and these clones were established and characterized to confirm as ES cells, as described previously . Established ES cells were maintained in a pluripotent state by culturing on an irradiated MEF feeder layer in media supplemented with 1000 U/ml of recombinant LIF (ESGRO, Chemicon International).
To produce embryoid bodies, approximately 6 × 106 ES cells were suspended in 10 ml of medium lacking LIF on non-adhesive Petri dishes and allowed to aggregate for 5 to 7 days. After the first two days, 5 ml of fresh medium was added to the plates. Afterward, the medium was then changed every other day by collecting the cell aggregates with brief centrifugation (2,000 g for 1 min) and re-suspending the aggregates in fresh medium by gently pipetting the aggregates. In some experiments, retinoic acid (1 μM) was added in the medium to enhance endoderm differentiation. At the end of each experiment, the cells aggregates were collected and processed for Western blot or histology analysis. The starting concentrations of ES cells were adjusted to ensure the resulting embryoid bodies are in a size range of 20–100 μm, which is comparable to the size of embryo cross sections at E5.5 stage.
At earlier stages (E5.5 and E4.5), dab2 genotyping was performed by examining the morphology and/or Dab2 immunostaining of the embryos to distinguish dab2 (+/+) or (+/-) from (-/-) genotypes.
For embryos at later stages (E9.5) and adult mice, DNA was extracted from entire embryo proper, yolk sacs, or tail biopsies. PCR amplification with primers 5′-TAC AGG CAT CCC CAT TTT TG -3′, 5′-TGC CAC CTA CAA GGA AGG AC-3′, and 5′-ACA GGC TGT GCA GTC TCG TA-3′ generated amplicons of 180 bp (dab2 wildtype, or wt), 284 bp (flox, or fl), and 529 bp (delta flox, or df). Cre alleles were amplified using the generic Cre primers: 5′-CCT GGA AAA TGC TTC TGT CCG -3′ and 5′-CAG GGT GTT ATA AGC AAT CCC-3′ to generate a 400 bp fragment.
Histology, histochemistry, and immunofluorescence microscopy
Embryos from timed matings were harvested either while in utero (up to E7.5) or as individually dissected embryos (E9.5). Part of the later stage embryos was used for genotyping. The collected specimens were processed by formalin fixation and paraffin embedding. The archived tissues were then sectioned and processed for histology and immunofluorescence microscopy.
Primary antibodies used in this study were: mouse monoclonal anti-Dab2 (BD Transduction Laboratories); rabbit polyclonal anti-Dab2 ; rabbit polyclonal anti-Gata4 antibodies (Santa Cruz Biotechnology, Inc.); rabbit pan anti-laminin (Abcam); rabbit polyclonal anti-GATA6 ; mouse monoclonal anti-Oct3/4 (Santa Cruz Biotechnology); mouse monoclonal anti-Arh (Santa Cruz Biotechnology, Inc.); goat polyclonal anti-Numb (Abcam), and mouse monoclonal anti-beta-actin (BD Transduction Laboratories). DAPI (4′-6-diamidino-2-phenylindole) was used as a generic nuclear counterstain and applied at the terminal stages of the procedure.
Conventional wide-field microscopy was performed on an inverted Zeiss AxioObserver Z1 operated by Axio Vision 4.8 software. Images were acquired digitally with a monochrome Zeiss AxioCam MRm CCD camera.
We appreciate the excellent technical assistance of Malgorzata Rula, Isabelle Roland, and Jennifer Smedberg, who have contributed to the preparation of the targeting construct and the production of the dab2 conditional knockout mice. We also acknowledge the technical assistance from Ms. Toni Yeasky. The work was supported by R01 CA095071, R01 CA79716, and R01 CA75389 to X.X. Xu from NCI, NIH. R. Moore was also funded by an institutional American Cancer Society grant and the Sylvester Comprehensive Cancer Center. The early stages in the production of Dab2 flox mutant mice were performed at the Fox Chase Cancer Center (Philadelphia, PA) and the completion and subsequent analyses were done at the University of Miami. We appreciate the assistance and contribution of Xiang Hua from the transgenic mouse facility, Sharon Howard of the cell culture facility, and Tony Lerro and Jackie Valvardi of the animal facility at Fox Chase Cancer Center for their help in making the mutant mice used in this study. We appreciate the assistance from Division of Veterinarian Resources and Animal Facility at the University Miller School of Medicine for maintaining the mouse colonies. We thank our colleagues, Jeffery Tse, Yue Meng, Santas Rosario, and James Hoy, for reading, suggestions, and comments on the manuscript.
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