Figure 4

Efficiency of dab2 deletion in embryonic and extraembryonic tissues by Sox2-Cre in E9.5 embryos. E9.5 embryos from a timed mating between female dab2 (fl/fl) and male dab2 (df/+);Sox2-Cre were dissected and harvested for analysis. (A) The example shows the region dissected as embryo (#1) and extraembryonic tissue (#2). (B) The embryonic (e) and extraembryonic (ex) tissues were separated and used for PCR genotyping of dab2 gene to determine the presence of Cre (400 bp), df (529 bp), fl (284 bp), and wildtype (wt, 180 bp) allele, to estimate the efficiency of dab2 gene deletion.