- Research article
- Open Access
Heat shock factor-1 intertwines insulin/IGF-1, TGF-β and cGMP signaling to control development and aging
BMC Developmental Biology volume 12, Article number: 32 (2012)
Temperature affects virtually all cellular processes. A quick increase in temperature challenges the cells to undergo a heat shock response to maintain cellular homeostasis. Heat shock factor-1 (HSF-1) functions as a major player in this response as it activates the transcription of genes coding for molecular chaperones (also called heat shock proteins) that maintain structural integrity of proteins. However, the mechanisms by which HSF-1 adjusts fundamental cellular processes such as growth, proliferation, differentiation and aging to the ambient temperature remain largely unknown.
We demonstrate here that in Caenorhabditis elegans HSF-1 represses the expression of daf-7 encoding a TGF-β (transforming growth factor-beta) ligand, to induce young larvae to enter the dauer stage, a developmentally arrested, non-feeding, highly stress-resistant, long-lived larval form triggered by crowding and starvation. Under favorable conditions, HSF-1 is inhibited by crowding pheromone-sensitive guanylate cyclase/cGMP (cyclic guanosine monophosphate) and systemic nutrient-sensing insulin/IGF-1 (insulin-like growth factor-1) signaling; loss of HSF-1 activity allows DAF-7 to promote reproductive growth. Thus, HSF-1 interconnects the insulin/IGF-1, TGF-β and cGMP neuroendocrine systems to control development and longevity in response to diverse environmental stimuli. Furthermore, HSF-1 upregulates another TGF-β pathway-interacting gene, daf-9/cytochrome P450, thereby fine-tuning the decision between normal growth and dauer formation.
Together, these results provide mechanistic insight into how temperature, nutrient availability and population density coordinately influence development, lifespan, behavior and stress response through HSF-1.
In the nematode Caenorhabditis elegans the insulin/IGF-1 and TGF-β signaling pathways co-regulate metabolism, aging, stress tolerance and development [1–5]. Harsh environmental conditions (e.g., starvation, high population density or high temperatures) and mutations that reduce the activity of either of these pathways cause lipid accumulation in the adipose tissues, lifespan extension, an enhanced tolerance against heat stress, and a switch in the developmental program from normal reproductive growth to dauer development (the dauer is an alternative third larval stage, a developmental diapause, in which the animal shifts metabolism and alters behavior in order to maximize survival and dispersal) [6, 7]. The membrane bound receptor guanylate cyclase (GC) DAF-11 (dauer formation constitutive; Daf-c) is an upstream regulator of both pathways. It generates the messenger molecule cGMP, which activates the TGF-β ligand DAF-7 and the insulin/IGF-1 ligand DAF-28 (Figure 1A) . Interestingly, mutations blocking insulin/IGF-1 or TGF-β signaling promote dauer development in a temperature-dependent fashion. For example, at 20°C mutant animals defective for DAF-2 (the worm IGF-1 receptor) or DAF-7 develop as dauer larvae with a relatively moderate penetrance. At 25°C, however, both single mutants develop exclusively as dauer larvae. Thus, expression of the Daf-c phenotype in daf 2(−) and daf 7(−) mutants relies on the perception of the ambient temperature. This implies that the effects of nutrient supply, population density and temperature are somehow orchestrated in the animal to influence reproductive growth, longevity, stress resistance and metabolism.
In divergent eukaryotic species, the transcription factor HSF-1 adjusts various cellular processes in response to heat stress through initiating a conserved transcriptional program [9–11]. Upon temperature increase, HSF-1 becomes activated via trimerization and phosphorylation, then translocates into the nucleus to promote the transcription of genes that encode heat shock proteins (HSPs) such as Hsp70, Hsp72, and Hsp90 [12–14]. These factors largely contribute to the protection of cells from protein-damaging stress. In C. elegans, insulin/IGF-1 signaling inhibits HSF-1 activity [9, 15]; DAF-2 was recently shown to inhibit phosphorylation of DDL-1 (DAF-16-dependent longevity) and DDL-2, two negative regulators of HSF-1, in order to keep HSF-1 in an inactive (the DHIC protein complex bound) form . When insulin/IGF-1 signaling is attenuated, HSF-1 and the insulin/IGF-1 signaling target DAF-16/FoXO (Forkhead box-O) stimulate the expression of genes required for longevity and stress resistance (Figure 1A) . Among their common targets, several small heat shock protein encoding genes can be found .
HSF-1 represses daf-7expression
Under favorable conditions, DAF-7/TGF-β accumulates in two chemosensory neurons, the ASIs, to promote reproductive growth cell non-autonomously . DAF-11/GC activity, which is inhibited by the crowding (dauer) pheromone DAF-22, leads to daf 7 upregulation (Figure 1A). Indeed, an integrated daf 7::gfp reporter (ksIs2) showed a strong ASI-specific expression in an otherwise wild-type, but only a faint expression in a daf 11(−) mutant genetic background (Figure 1B, C). We found that HSF-1 function is required for daf 7 repression in animals defective for DAF-11: daf 11(−); hsf 1(−) double mutants displayed nearly wild-type (i.e., strong) levels of daf 7 expression (Figure 1B, C). A similar result was observed with variable penetrance in daf 11(−) mutant animals depleted for HSF-1 [on average, 82% of daf 11(m47); hsf 1(RNAi) animals showed wild-type levels of daf 7 expression; N=233]. These results suggest that HSF-1 mediates the regulatory effect of DAF-11 on daf 7 transcription, and that HSF-1 represses daf 7.
daf 11 shares many functions with daf 21 encoding an Hsp90 chaperone; daf 11 and daf 21 have similar epistasis relationships with other genes in the dauer pathway (Figure 1A) [8, 17]. A daf 21 allele, p673, causes a massive downregulation of daf 7 expression in the ASIs. This prompted us to monitor whether inactivation of HSF-1 suspends daf 7 repression in daf 21(p673) mutants, too. Expression of daf 7 in the daf 21(p673); hsf 1(sy441) double mutant genetic background was indeed as strong as in the wild-type background (Figure 1B, C).
To confirm the results above, we assayed dauer formation in daf-11(−) single mutant versus daf-11(−); hsf-1(−) double mutant animals, and found that HSF-1 deficiency strongly suppresses dauer development in daf-11(−) genetic background (Figure 1D). Thus, HSF-1 activity is required for dauer formation in daf-11(−) mutants animal. Dauer development in daf-21(p673) mutant animals was also blocked by the hsf-1(sy441) mutation [the amorph hsf-1(ok600) mutation arrests development at the L1/L2 larval stages, therefore hsf-1(ok600) mutants were excluded from the dauer formation assays] (Figure 1D). DAF-11/GC and DAF-21/Hsp90 hence upregulate daf-7 through inhibiting its upstream transcriptional repressor, HSF-1. We suggest that HSF-1 is a target of cGMP signaling, and links the cGMP and TGF-β pathways in development control.
Next, we measured daf-7 transcript levels by quantitative RT-PCR. Levels of daf-7 mRNA were reduced by half when animals were grown at 27°C, as compared with those maintained at 20°C (Figure 1D). High temperature-induced downregulation of daf-7 was abolished by the hsf-1(sy441) mutation, suggesting that HSF-1 mediates the modulatory effect of temperature on daf-7 transcription. Moreover, hyperactivation of HSF-1 decreased daf-7 transcriptional activity by half at 20°C (Figure 1E). These data imply that DAF-11 and DAF-21 negatively regulate HSF-1, whose activity in turn represses daf-7 expression (Figure 1F).
As HSF-1 acts as a transcription factor, we next asked whether it directly influences daf 7 activity. Our genome-wide sequence analysis identified a canonical HSF-1 binding site (GAANNTTCNNGAA, see Ref. 1) in the regulatory region of daf 7, 278 base pairs (bp) upstream of the ATG translational initiation site (Figure 2A) (previously, this approach effectively identified substantive DNA-protein interactions; [18–20]). This site is highly conserved in the daf 7 genomic region of closely related Caenorhabditis species (Figure 2B). We generated a transcriptional fusion daf 7::gfp (green fluorescent protein) reporter and a mutated version of this reporter in which 6 critical bases were removed from the canonical HSF-1 binding site (p mut daf 7::gfp) (Figure 2C). Both reporters were strongly expressed in the ASIs (Figure 2D). However, while the wild-type reporter was downregulated in daf 11(−) single mutants and remained strong in daf 11(−); hsf 1(−) double mutants, expression of the mutant reporter was not significantly changed upon DAF-11 deficiency (Figure 2D). These results provide an in vivo evidence for the functionality of this particular HSF-1 binding site in the daf 7 promoter. We conclude that HSF-1 acts together with DAF-11, DAF-21 and DAF-7 along the same signaling axis to control reproductive growth. In this cascade DAF-11 and DAF-21 inhibit HSF-1, thereby stimulating daf 7 expression. Under dauer-inducing conditions, HSF-1 becomes active and represses daf 7. This epistasis model (Figure 1F) may explain why nematodes can develop as dauer larvae at 27°C even when they are well-fed and propagated at a low population density .
HSF-1 also acts downstream of DAF-7/TGF-β to regulate aging and development
TGF-β signaling influences longevity via the insulin/IGF-1 signaling pathway; loss-of-function mutations in daf 7 and daf 4 (daf 4 encodes a type I TGF-β receptor; Figure 1A) can double the animal’s natural lifespan and this longevity effect requires DAF-16 activity . Signaling through DAF-7 negatively regulates the Smad-like transcriptional cofactor DAF-3 (Figure 1A). In the absence of DAF-7 activity, DAF-3 inhibits certain insulin-like peptides and upregulates other insulin-like peptides (indicated by the green broken bar in Figure 1A) to decrease DAF-2/IGF-1 function and extend lifespan [5, 22]. As HSF-1 is required for longevity in insulin/IGF-1 signaling deficient animals [1, 9], we hypothesized that it mediates lifespan extension in daf 7(−) mutants as well. Indeed, the long-lived phenotype of daf 7(e1372) mutant animals was strongly, but not completely, suppressed by the hsf 1(sy441) mutation (Figure 3). daf 7(e1372); hsf 1(sy441) double mutants lived nearly as short as hsf 1(sy441) single mutants. This raises the possibility that HSF-1 functions downstream of DAF-7 in ageing control. It is worth to note that the incomplete suppression of longevity in daf 7(e1370) mutants by the hsf 1(sy441) mutation may result from the fact that sy441 represents a hypomorphic, and not a genetic null, allele.
Dauer formation in daf 7(−) null mutant animals correlates with the ambient temperature: the higher the temperature is between 15-25°C, the larger proportion of the population enters the dauer diapause . This phenomenon prompted us to examine whether dysregulation of HSF-1 modulates the ability of these mutants to develop as dauer larvae. We found that inactivation of HSF-1 increases, while its hyperactivation decreases the penetrance of the Daf-c phenotype of DAF-7 deficient animals (Figure 4A). A series of daf 7 mutant alleles, including e1372, ok3125 and m62, exhibited an HSF-1-dependent ability to induce dauer development at 20°C. These results imply that HSF-1 acts both upstream and downstream of DAF-7 to modulate the developmental choice between reproductive growth and dauer formation. First, it promotes dauer development by acting downstream of daf 11 and daf 21 to repress daf 7 expression (the upstream effect). Thus, hsf 1 functions between daf 11/daf 21 and daf 7 in the genetic (TGF-β) pathway that regulates dauer development. Second, HSF-1 inhibits dauer development through interacting with (a) downstream component(s) of the TGF-β pathway (the downstream effect). Interestingly, a previous study also reported that depletion of HSF-1 significantly modulates the percentage of dauer larvae in daf 7(−) mutant populations . However, the authors showed that RNAi-mediated knockdown of HSF-1 suppresses, rather than enhances (what actually happened in our experiments), the Daf-c phenotype of daf 7(−) mutant animals. This discrepancy may result from specific RNAi conditions used in that early study. For example, daf 7(e1372) mutants (e1372 is an amorph mutation) maintained at 25°C could enter the dauer stage with only 20%, although numerous other studies reported that these mutant animals develop as dauer larvae exclusively under identical conditions.
HSF-1 upregulates daf-9/cytochrome P450
Our data above indicate that HSF-1 may control the expression of a TGF-β component that functions downstream of DAF-7 to affect development. A good candidate for this component is daf 9 that codes for a cytochrome P450 [6, 7]. The cytochrome P450 superfamily of enzymes is involved in the metabolism of a large number of organic substances, including synthesis and breakdown of steroid hormones. DAF-9 modulates the activity of the TGF-β dauer formation pathway by inhibiting the nuclear hormone receptor DAF-12 (Figure 1A). Interestingly, daf 9, which determines two DAF-9 isoforms, is known to be expressed in the hypodermis at early larval stages in a temperature dependent manner (fluorescent images in Figure 4) . Consistent with these results, further sequence analysis identified a conserved HSF-1 binding site in the second intron of daf 9, which actually is located in the upstream regulatory region of the shorter daf 9 isoform (Figure 4B). This conserved site is also present in the C. briggsae and C. remanei daf 9 loci (Figure 4C). A daf 9::gfp reporter system (dhEx67; Ref. 6) containing this potential HSF-1 binding element displayed a faint hypodermal expression in wild-type, but a strong activity in daf 11(−) mutant background (as DAF-11 deficiency hyperactivates HSF-1) (Figure 4D). Upregulation of daf 9 in daf 11(−) mutant animals was, however, suppressed by the hsf 1(sy441) mutation, suggesting that HSF-1 mediates temperature-induced stimulation of daf 9. As DAF-11 accumulates in certain amphid neurons in the head, its negative regulatory effect on the hypodermal daf 9 expression should occur cell non-autonomously. Together, daf 9 may serve as another transcriptional target for HSF-1, and HSF-1 modulates the effect of TGF-β signaling on larval development at multiple points.
DAF-2/IGF-1 promotes daf-7expression via inhibition of HSF-1
Insulin/IGF-1 signaling inhibits HSF-1 through modulating the phosphorylation status, and thereby the activity, of the HSF-1 regulator DDL-1/2 proteins . This interaction raises the possibility that DAF-2 also promotes daf 7 expression via HSF-1 (as HSF-1 represses daf 7). To address this issue we first assayed daf 7 expression in daf 2(−) mutant dauer larvae (Figure 5A). In these animals, daf 7 was expressed in the ASIs at relatively low levels. In contrast, impairment of HSF-1 function strongly induced daf 7 expression in the ASIs and caused a strong ectopic daf 7 expression in neurons of the ventral nerve cord (Figure 5A). Excessive and ectopic expression of daf 7 was obvious in each hsf 1(−) mutant dauer larva examined. We next analyzed daf 7 activity in wild-type versus daf 2(−) mutant background at the L1 stage, and found that daf 7 expression is downregulated upon DAF-2 deficiency (Figure 5B, C). Although a reduction in gfp glowing was evident in daf 2(−) mutant L1 larvae, this expressional change was less robust than in daf 11(−) mutant L1 larvae. Based on these data one can speculate that DAF-2 only reduces, but does not completely eliminate HSF-1 activity. We also tested the modulatory effect of hsf 1(sy441) mutation on daf 7 expression in daf 2(−) mutant background. Expression levels of daf 7 in daf 2(−); hsf 1(−) double mutants were as nearly strong as in the wild-type background (Figure 3B). This suggests that HSF-1 mediates—via two sequential inhibitory steps—stimulation of daf 7 by DAF-2 (Figure 5B, C). Accordingly, the proportion of dauer larvae in daf 2(−) mutant populations maintained at 20-23°C was significantly enhanced by HSF-1 hyperactivity (Figure 6). In accordance with previously published data , these results point to a regulatory link through which nutritional status of the animal can influence developmental decision between normal reproductive growth and dauer larva formation in a temperature-dependent fashion (Figure 5D).
HSF-2 promotes reproductive growth
The C. elegans genome encodes an HSF-1 paralog, HSF-2 (the WormBase database; http://www.wormbase.org). hsf-2 (the ORF Y53C10A.3) is predicted to be composed of 4 exons (Figure 7A). tm4607 is a deletion allele of hsf-2 which removes nearly 200 bp of upstream regulatory sequences and most part of the first exon. hsf-2(tm4607) mutant hermaphrodites were backcrossed with wild-type males 4 times, and then subjected to a phenotypic characterization at 25 and 27°C. According to our data, the vast majority of hsf-2(tm4607) mutant L1 larvae developed into adulthood at both temperatures, i.e. they did not exhibited a high temperature-induced dauer formation constitutive (Hid) phenotype. Next, we generated an hsf-2(tm4607); daf-11(m47) double mutant strain, and found a significant increase in dauer larva formation in these animals, as compared with daf-11(m47) single mutants (Figure 7B). Thus, unlike HSF-1, HSF-2 inhibits dauer development. Since we do not know whether HSF-2 also functions as a transcription factor, further experiments are needed to clarify the molecular nature of this inhibition.
Inhibitory mutations in unc 3 (uncoordinated) and unc 31 cause a Hid phenotype: the mutant animals do not form dauers at 25°C, but develop as dauer larvae at 27°C . UNC-31 and UNC-3 function upstream of the insulin/IGF-1 and TGF-β signaling pathways, respectively. We assessed whether the hsf 2(tm4607) mutation affects dauer formation in unc 3(−) and unc 31(−) mutant animals (Figure 7C). The tm4607 allele had no influence on dauer development in unc 31(−) mutants, but significantly increased the ability of unc 3(−) mutant animals to enter the dauer stage. This synergism suggests that hsf 2 acts in parallel to unc 3, probably upstream of unc 31, to promote dauer development (Figure 7D). At the moment, however, we cannot exclude the possibility that hsf 2 may function upstream of daf 11 (indicated by the question mark in Figure 7D). Further epistasis analysis combining hsf 2(tm4607) with ins(−), daf 2(−) and daf 16(−) mutations would be required to place hsf 2 unambiguously in this signaling network.
Most of the HSF-1 targets identified so far encode heat shock proteins protecting cells from protein-damaging agents . A few examples where HSF-1 transcriptionally represses non-heat shock proteins were also reported [24, 25]. In this study we identified two C. elegans TGF-β pathway-related genes, daf 7/TGF-β and daf 9/cytochrome P450, whose regulatory regions contain a conserved binding site for HSF-1 (Figures 2A, B and 4B, C), and whose expression highly depends on HSF-1 activity (Figures 1B, C, 2D and 4D). daf 7 and daf 9 are likely to be under the direct control of HSF-1. daf 7 expression is repressed, while daf 9 expression is upregulated, by HSF-1, resulting in opposite effects on dauer formation. The overall impact of (dual) HSF-1 activity on this developmental choice (reproductive growth vs. dauer larva formation) is to promote dauer development in the wild type at high temperatures (over 27°C) with only a moderate percentage. Consistent with these results, another cytochrome P450 encoding gene, cyp35B1/dod 13, was recently identified as a HSF-1 target gene . HSF-1 also upregulates cyp35B1/dod 13 expression in intestinal cells . Taken together, our present results further suggest that the HSF-1-initiated transcriptional program involves up- and downregulation of “metabolic” genes, the protein products of which have no chaperone function. Rather, these proteins operate as key components of signal transduction pathways implicated in metabolism, development and lifespan control. In the light of these data, one can mechanistically explain how upshift in the ambient temperature affects—in addition to protein homeostasis—diverse cellular processes such as growth, proliferation, fat metabolism, differentiation and aging. Nevertheless, once the developmental decision favoring dauer larva formation is made, several hsp genes become also upregulated by HSF-1, and their products contribute to increased stress resistance in the animal.
Our data presented here indicate that HSF-1 represses daf-7 through a direct transcriptional interaction (Figure 2). daf-7 codes for a TGF-β ligand that activates the TGF-β signaling axis promoting reproductive growth. daf-7 regulation by HSF-1 elucidates why nematodes can enter into the dauer stage at high (27°C) temperature even when they are well-fed and not crowded. Moreover, HSF-1 activity is influenced by the receptors DAF-11 and DAF-2 (Figures 1, 5 and 6). DAF-11 is responsive to concentration of the dauer pheromone (i.e., to population density), while DAF-2 senses the animal’s nutritional status. Nematodes deficient in either of these proteins form dauer larvae and age in a temperature-dependent manner. For example, daf-2(−) mutant animals develop reproductively at 15°C, but develop as dauer larvae at 25°C. More significantly, daf-2(−) mutants that reach the adulthood live much longer at 15°C than at 25°C. Thus, a complex regulatory interaction mediated by HSF-1 among the TGF-β, guanylate cyclase/cGMP and insulin/IGF-1 signaling systems exists to affect development and lifespan in response to various environmental factors, including population density, nutrient availability and temperature.
HSF-1 stimulates daf 9/cytochrome P450 activity (Figure 4B-E), implying that it acts both upstream and downstream of DAF-7 to control nematode development (Figures 4E, 7D). daf 7 repression by HSF-1 promotes, while daf 9 upregulation by HSF-1 suppresses dauer formation under a given condition. Why is a dual function of HSF-1 needed for dauer formation control? At 27°C, only a minor portion (<2-5%) of C. elegans populations enters the dauer stage . Without stimulating daf 9 activity, HSF-1 would completely inhibit reproductive growth. Actually, this occurs in sensitized [e.g., daf 2(−) and daf 11(−)] mutant genetic backgrounds. Since in the temperate and tropical zones the temperature is often above 27°C, nematode populations would frequently exist as non-reproducing dauer larvae under these conditions. This way, HSF-1 regulates both a dauer-inhibiting (daf 7) and a dauer-inducing (daf 9) TGF-β pathway component in order to allow the populations to survive (some dauer larvae) and propagate (many reproductive adults) simultaneously under environmental stress, thereby maximizing the chance of the population to survive.
Finally, here we revealed that HSF-1 mediates the regulatory effect of DAF-11 on C. elegans development. DAF-11 is a receptor guanylate cyclase that is implicated in chemosensation and behavior . Interestingly, several behavioral patterns in various animal species depend on the ambient temperature. Our present finding that guanylate cyclase acts through HSF-1 in controlling C. elegans dauer formation may help to understand how heat stress, food deprivation or crowding also affects behavior in humans.
Fundamental insights into how genes and environmental factors influence metazoan metabolism, development and aging have emerged from a genetic dissection of the C. elegans dauer diapause, which is an arrested (non-reproductive), long-lived, and highly stress-resistant larval form triggered by starvation and crowding. Identified molecular pathways that regulate the developmental choice between reproductive growth and dauer larva formation include the insulin/IGF-1, TGF-β and guanylate cyclase/cGMP neuroendocrine systems. Here we show that heat shock factor-1 (HSF-1), a major player in cellular response to heat stress, intertwines these signaling systems in development and aging control. Under adverse environmental conditions HSF-1 promotes dauer larva development through repression of daf-7, which encodes a TGF-β ligand. When conditions are favorable, HSF-1 is inhibited by crowding pheromone-sensitive guanylate cyclase/cGMP and systemic nutrient-sensing insulin/IGF-1 signaling; loss of HSF-1 activity allows DAF-7 to induce reproductive growth. These results provide mechanistic insight into how temperature, nutrient availability and hormonal factors coordinately influence development, stress response, behavior and lifespan through HSF-1. In humans, an orthologous HSF-1-mediated signaling system may be dysregulated in diabetes, cancer and obesity.
Strains and alleles
The wild-type strain corresponds to var. Bristol (N2). The following mutant and transgenic strains were used in this study: PS3551 hsf-1(sy441)I, FX04607 hsf-2(tm4607)I, CB1370 daf-2(e1370)III, CB1372 daf-7(e1372)III, RB2302 daf-7(ok3125)III, DR62 daf-7(m62)III, DP38 unc-119(ed3)III, DR47 daf-11(m47)V, PR673 daf-21(p673)V, CB169 unc-31(e169)IV, CB151 unc-3(e151)X, FK181 ksIs2[pdaf-7::gfp + rol-6(su1006)], VC3071 hsf-1(ok600)/hIn1[unc-101(sy241)]I, CF1824 muEx265[hsf-1p::hsf-1cDNA + myo-3::GFP], TTV200 bjIs10[hsf-1p::hsf-1cDNA + myo-3::GFP] backcrossed 4x, TTV201 hsf-2(tm4607)I backcrossed 4x, TTV116 bjEx1[pdaf-7::daf-7::gfp + unc-119(+)], TTV117 bjEx9[pmutdaf-7::daf-7::gfp + unc-119(+)].
Generation of pdaf-7::gfp and pmutdaf-7::gfptransgenic strains
To generate a pdaf-7::gfp reporter, a genomic fragment containing 3,8 kb of the 5’ regulatory region and the first exon of daf-7 was amplified by the following forward and reverse primers: 5’-aaa tcc gag tcc gtg aaa tg-3’ and 5’-aaa cac cgg gag tga aga tg-3’. The resulting fragment was cloned into pGEM®-T Easy vector and subcloned into the vector pPD95.75, using the SphI and SalI restriction enzymes. QuikChange® II Site-Directed Mutagenesis Kit (Stratagene) was used to generate the mutant version of this reporter, with the primers: 5'-caa ttc cgc aaa att ttc tgg ctt ttc tct acg gta tag atg-3' and 5'-cat cta tac cgt aga gaa aag cca gaa aat ttt gcg gaa ttg-3’. These daf-7 genomic fragments were inserted in frame with gfp in these constructs. Transgenic strains were generated by biolistic transformation using Biolistic PDS-1000/He particle delivery system (BioRad). 10-15μg linearized plasmid DNA was bombarded onto unc-119(ed3) mutant L4/adult hermaphrodites. Fluorescent images were taken by an Olympus BX51 epifluorescence microscope.
Quantification of GFP expression intensity
Measurements were performed by the Image J software. Briefly, images were captured with Olympus BX51 epifluorescence microscope with a given exposure time. Cells of interest were selected, and mean grey values were measured. Fluorescent intensity of selected area was calculated by subtracting mean grey value of the background from mean grey value of the object of interest (the same size of areas was selected).
Life span assays were carried out at 25°C, as described previously . Briefly, all strains were maintained at 20°C until the L4 larval stage, then transferred at 25°C, and scored for mean life span. For synchronization, 20–30 gravid well-fed adults were transferred to a new agar plate containing nematode growth medium (NGM) seeded with E. coli OP50 to lay eggs for 4–5 hours, and then removed. F1 young adults were transferred to NGM plates supplemented with 300μg/μl FUDR (5-fluoro-2'-deoxyuridine). Animals were considered dead when they stopped pharyngeal pumping and responding to touching. SPSS software was used to calculate mean life span and perform statistical analysis. P values for comparing Kaplan-Meyer survival curves between two groups were determined using log-rank (Mantel-Cox) tests.
Quantification of daf-7mRNA levels (qRT-PCR)
RNA samples were extracted from 100–200 synchronized L1 larvae, using a PureLink™ Micro-to-Midi Total RNA Purification System (Invitrogene). RNase-free DNase I (Fermentas) was used to eliminate genomic DNA contamination. DNA-free RNA samples were then converted to cDNA, using random hexamers by RevertAid First Strand cDNA Synthesis Kit. Amplification reactions were carried out in a total volume of 20 μl, using LightCycler® FastStart DNA Master SYBR Green I. The reaction conditions for the LightCycler® 2.0 Instrument (Roche) were: 95°C for 5 min, followed by 45 cycles of 95°C for 10s, 60°C for 5s, 72°C for 10s. Fluorescence was measured at each cycle at 72°C. Melting curve analysis was performed after PCR to assess the presence of a unique final product. Gene expression data are presented as the fold change in mRNA transcript abundance in mutant strains, normalized to one endogenous reference gene (pmp-3), relative to the wild-type strain. Primers for the housekeeping gene pmp-3: 5’-gtt ccc gtg ttc atc act cat-3’and 5’-aca ccg tcg aga agc tgt aga-3’, and for daf-7: 5’-caa caa tgt gat agg caa cga-3’and 5’-aac tac gca cgc aca gac ac-3’. The results represent the mean of 3 independent experiments. To compare the means of dauer progeny, variables were analyzed by Student’s t-test. Results are expressed as mean ± S.E.M.
5–10 gravid adults were allowed to lay eggs for 4–6 hours at 20°C to obtain a synchronous population. Plates were placed to the assaying temperatures (20°C, 23°C, 26,5°C), and percentage of dauer larvae was scored typically after 72, 60 and 44 hours, respectively. At least three plates per strain were assayed. Results are expressed as mean ± S.E.M. Data were analyzed by one-way ANOVA and student t-test.
An hsf-1 cDNA clone (yk1245f10, a gift of Yuji Kohara) was cut by HindIII and KpnI, and the resulting 1333bp-long cDNA fragment was cloned into the L4440 “feeding” vector. The construct was then transformed into the bacterial strain Escherichia coli HT115(DE3).
Hsu AL, Murphy CT, Kenyon C: Regulation of aging and age-related disease by DAF-16 and heath shock factor. Science. 2003, 300: 1142-1145. 10.1126/science.1083701.
Kenyon CJ: The genetics of ageing. Nature. 2010, 464: 504-512. 10.1038/nature08980.
Matyash V, Entchev EV, Mende F, Wilsch-Bräuninger M, Thiele C, Schmidt AW, Knölker HJ, Ward S, Kurzchalia TV: Sterol-derived hormone(s) controls entry into diapause in Caenorhabditis elegans by consecutive activation of DAF-12 and DAF-16. PLoS Biol. 2004, 2: e280-10.1371/journal.pbio.0020280.
Ogg S, Paradis S, Gottlieb S, Patterson GI, Lee L, Tissenbaum HA, Ruvkun G: The Fork head transcription factor DAF-16 transduces insulin-like metabolic and longevity signals in C. elegans. Nature. 1997, 389: 994-999. 10.1038/40194.
Shaw WM, Luo S, Landis J, Ashraf J, Murphy CT: The C. elegans TGF-beta Dauer pathway regulates longevity via insulin signaling. Curr Biol. 2007, 17: 1635-1645. 10.1016/j.cub.2007.08.058.
Gerisch B, Antebi A: Hormonal signals produced by DAF-9/cytochrome P450 regulate C. elegans dauer diapause in response to environmental cues. Development. 2004, 131: 1765-1776. 10.1242/dev.01068.
Jia K, Albert PS, Riddle DL: DAF-9, a cytochrome P450 regulating C. elegans larval development and adult longevity. Development. 2002, 129: 221-231.
Bargmann CI: Chemosensation in C. elegans. WormBook. 2006, 25: 1-29.
Morley JF, Morimoto RI: Regulation of longevity in Caenorhabditis elegans by heat shock factor and molecular chaperones. Mol Biol Cell. 2004, 15: 657-664.
Prahlad V, Morimoto RI: Integrating the stress response: lessons for neurodegenerative diseases from C. elegans. Trends Cell Biol. 2009, 19: 52-61. 10.1016/j.tcb.2008.11.002.
Westerheide SD, Anckar J, Stevens SM, Sistonen L, Morimoto RI: Stress-inducible regulation of heat shock factor 1 by the deacetylase SIRT1. Science. 2009, 323: 1063-1066. 10.1126/science.1165946.
Wu C: Heat shock transcription factors: structure and regulation. Annu Rev Cell Dev Biol. 1995, 11: 441-469. 10.1146/annurev.cb.11.110195.002301.
Xu D, Zalmas LP, La Thangue NB: A transcription cofactor required for the heat-shock response. EMBO Rep. 2008, 9: 662-669. 10.1038/embor.2008.70.
Zhou X, Tron VA, Li G, Trotter MJ: Heat shock transcription factor-1 regulates heat shock protein-72 expression in human keratinocytes exposed to ultraviolet B light. J Invest Dermatol. 1998, 111: 194-198. 10.1046/j.1523-1747.1998.00266.x.
Chiang WC, Ching TT, Lee HC, Mousigian C, Hsu AL: HSF-1 regulators DDL-1/2 link insulin-like signaling to heat-shock responses and modulation of longevity. Cell. 2012, 148: 322-334. 10.1016/j.cell.2011.12.019.
Ren P, Lim CS, Johnsen R, Albert PS, Pilgrim D, Riddle DL: Control of C. elegans larval development by neuronal expression of a TGF-beta homolog. Science. 1996, 274: 1389-1391. 10.1126/science.274.5291.1389.
Birnby DA, Link EM, Vowels JJ, Tian H, Colacurcio PL, Thomas JH: A transmembrane guanylyl cyclase (DAF-11) and Hsp90 (DAF-21) regulate a common set of chemosensory behaviors in Caenorhabditis elegans. Genetics. 2000, 155: 85-104.
Takács-Vellai K, Vellai T, Chen EB, Zhang Y, Guerry F, Stern MJ, Müller F: Transcriptional control of Notch signaling by a HOX and a PBX/EXD protein during vulval development in C. elegans. Dev Biol. 2007, 302: 661-669. 10.1016/j.ydbio.2006.09.049.
Szabó E, Hargitai B, Regős A, Tihanyi B, Barna J, Borsos E, Takács-Vellai K, Vellai T: TRA-1/GLI controls the expression of the Hox gene lin-39 during C. elegans vulval development. Dev Biol. 2009, 330: 339-348. 10.1016/j.ydbio.2009.04.005.
Hargitai B, Kutnyánszky V, Blauwkamp TA, Steták A, Csankovszki G, Takács-Vellai K, Vellai T: xol-1, the master sex-switch gene in C. elegans, is a transcriptional target of the terminal sex-determining factor TRA-1. Development. 2009, 136: 3881-3887. 10.1242/dev.034637.
Ailion M, Thomas JH: Dauer formation induced by high temperatures in Caenorhabditis elegans. Genetics. 2000, 156: 1047-1067.
Liu T, Zimmerman KK, Patterson GI: Regulation of signaling genes by TGFβ during entry into dauer diapause in C. elegans. BMC Dev Biol. 2004, 4: 11-10.1186/1471-213X-4-11.
Walker GA, Thompson FJ, Brawley A, Scanlon T, Devaney E: Heat shock factor functions at the convergence of stress response and developmental pathways in Caenorhabditis elegans. FASEB J. 2003, 17: 1960-1962.
Wang J, He H, Yu L, Xia HH-X, Lin MCM, Gu Q, Li M, Zou B, An X, Jiang B, Kung H-F, Wong BCY: HSF1 downregulates XAF1 through transcriptional regulation. J Biol Chem. 2006, 281: 2451-2459.
Xie Y, Zhong R, Chen C, Calderwood SK: Heat shock factor 1 contains two functional domains that mediate transcriptional repression of the c-fos and c-fms genes. J Biol Chem. 2003, 278: 4687-4698. 10.1074/jbc.M210189200.
Iser WB, Wilson MA, Wood WH, Becker K, Wolkow CA: Co-regulation of the DAF-16 target gene, cyp35B1/dod-13, by HSF-1 in C. elegans dauer larvae and daf-2 insulin pathway mutants. PLoS One. 2011, 6: e17369-10.1371/journal.pone.0017369.
Tóth ML, Sigmond T, Borsos E, Barna J, Erdélyi P, Takács-Vellai K, Orosz L, Kovács AL, Csikós G, Sass M, Vellai T: Longevity pathways converge on autophagy genes to regulate life span in Caenorhabditis elegans. Autophagy. 2008, 4: 330-338.
We thank A. Antebi, the Caenorhabditis Genetics Center funded by NIH and Shohei Mitani (National Bioresource Project, Japan) for nematode strains. This work was supported by the OTKA Hungarian Scientific Research Fund (Grants NK78012 and K75843) and the Central Hungary Operational Program (KMOP_1.1.1.-09/1-2009-0045). K. T-V. and T.V. are grantees of the János Bolyai Scholarship of the Hungarian Academy of Sciences.
The authors declare that they have no competing interests.
JB, KTV and TV designed research; JB, AP, MK, BH and KTV performed research; JB, KTV and TV analyzed data; JB and TV wrote the paper. All authors read and approved the final manuscript.
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Barna, J., Princz, A., Kosztelnik, M. et al. Heat shock factor-1 intertwines insulin/IGF-1, TGF-β and cGMP signaling to control development and aging. BMC Dev Biol 12, 32 (2012). https://doi.org/10.1186/1471-213X-12-32
- Heat shock factor-1
- C. elegans
- Dauer development
- Signaling crosstalk