Early development of the root-knot nematode Meloidogyne incognita
© Calderón-Urrea et al. 2016
Received: 8 January 2016
Accepted: 15 April 2016
Published: 28 April 2016
Detailed descriptions of the early development of parasitic nematodes are seldom available. The embryonic development of the plant-parasitic nematode Meloidogyne incognita was studied, focusing on the early events.
A fixed pattern of repeated cell cleavages was observed, resulting in the appearance of the six founder cells 3 days after the first cell division. Gastrulation, characterized by the translocation of cells from the ventral side to the center of the embryo, was seen 1 day later. Approximately 10 days after the first cell division a rapidly elongating two-fold stage was reached. The fully developed second stage juvenile hatched approximately 21 days after the first cell division.
When compared to the development of the free-living nematode Caenorhabditis elegans, the development of M. incognita occurs approximately 35 times more slowly. Furthermore, M. incognita differs from C. elegans in the order of cell divisions, and the early cleavage patterns of the germ line cells. However, cytoplasmic ruffling and nuclear migration prior to the first cell division as well as the localization of microtubules are similar between C. elegans and M. incognita.
The root-knot nematode Meloidogyne incognita (Tylenchida) is an economically important plant parasite with a wide host range, and abundant field populations can develop quickly under appropriate conditions. This rapid population growth is mainly due to the completion of several generations during a single growing season, combined with the high females fecundity. The exact number of eggs produced varies depending on environmental conditions. Under favorable conditions, a single female may produce 500–2000 eggs . The eggs have transparent protective chitin-containing shells and are deposited by the female in a desiccation resistant gelatinous matrix secreted by the female. Although males do exist, reproduction occurs exclusively via mitotic parthenogenesis (apomixis) .
Since there is no sperm contribution during reproduction in M. incognita, questions concerning determination of axis polarity and early events during development are very important, particularly considering the fact that these events are significant during the evolution of developmental patterns [3, 4]. In C. elegans, upon fertilization, the egg continues through meiosis, eventually leading to formation of the zygote and the first zygotic cellular division. The position of sperm entry into the oocyte designates the future posterior region of the developing embryo . The sperm also brings the centrosome needed for cell division, and paternal pronucleus that are required to carry out the formation of the zygote . The centrosome is required for microtubule formation, and is also needed to position the pronuclei for fusion. After the sperm enters, a series of events occurs that organize the cell. Among these are cytoplasmic ruffling and nuclear migration. In C. elegans cytoplasmic ruffling occurs after the moment of fertilization. This process involves movement of cytoplasmic material from the posterior side of the egg to the anterior region, or vice versa . Inside the C. elegans early embryo, which at this point is called a Po cell, there are a series of movements referred to as cortical flows, which appear physically as pseudocleavages and invaginations in the cell . Cortical flow is a result of contractions of the cytoskeleton, which move PAR proteins, such as PAR-3, in the anterior direction, establishing cell polarity . PAR-3 begin to locate to the anterior region [9, 10], while PAR-2 and P-granules move towards the posterior region, which was defined as such when the sperm entered the egg in that region . PAR-3 and PAR-2 proteins thus define the boundary of the anterior and posterior region of the single-celled embryo .
One of the major differences between C. elegans and M. incognita is the role of the sperm. Although sperm is not required for initiation of embryogenesis in C. elegans, a sexually reproducing nematode, the sperm does provide the centrosome, which is required for the first cell division. Furthermore, sperm entry is associated with proper positioning, determining anteriorposterior (A-P) polarity and leading to the future asymmetric cell division. It is not currently known what triggers development in the eggs off M. incognita. To better understand M. incognita’s reproductive mechanism, several cytological events during gametogenesis and oogenesis have been studied in detail (see ). In a SEM study, distinct developmental stages of embryo development could be distinguished . Another study followed the early stages of development of M. incognita, along with 36 other species of Nematoda, in order to provide a survey of early development for phylogenetic purposes; this early work reports that M. incognita has a synchronous pattern of development (i. e. the four blastomeres present are the same generation), that the first four blastomeres have the same size, and that they organize in tandem . However, there are no previous studies that investigated early cell lineages, including the timing of specific developmental events. This is mainly due to both the within-gall inaccessibility of this obligatory parasite and its slow development, making observations cumbersome and time consuming. In this study we documented the early developmental events of M. incognita, using 4D-microscopy, to draw comparisons with the well-characterized development of C. elegans.
Culturing nematodes and collecting eggs
Susceptible tomato plants (Rutger’s Select, Tomatoes, Augusta, GA) were grown in 0.5 l autoclaved white sand amended with slow release fertilizer (Osmocote® 19-6-12 formula, The Scotts Company LLC). The plants were grown at 28 °C with a 16 h light/8 h dark photoperiod and watered daily. Approximately 1-week-old tomato plants (10 cm shoot and approximately 4–5 cm long roots) were infected with pre-parasitic juveniles of M. incognita (race 1). The roots of an infected tomato plant (8–10 weeks post infection) were washed free of soil and heavily galled roots were gently chopped in M9 buffer (90 mM Na2HPO4, 22 mM KH2PO4, 9 mM NaCl and 19 mM NH4Cl) to release the eggs, shaken vigorously for 5 min with 10 % bleach, and subsequently poured through a 250 μm mesh screen. Eggs were collected from the flow-through on a 25 μm mesh screen and further purified by centrifugation for 10 min on a 35 % sucrose gradient at 500 × g. The egg-containing fraction was then subjected to two 10 min treatments in 10 % bleach followed by centrifugation at 500 × g for 5 min and several rinses in sterile distilled (DI) water.
Eggs from one infected tomato plant were harvested as described, observed with an inverted compound microscope and isolated using a drawn-out Pasteur pipette. The selected eggs were transferred to a microscope slide carrying a thin 5 % agar pad. The eggs were covered with a coverslip and sealed with petroleum jelly.
Approximately 105 fresh embryos were fixed in Histochoice Tissue Fixative MB (Amresco, Solon, OH) for 2 h and cleared in Histochoice Clearing Agent (Amresco, Solon, OH). 4',6-Diamidino-2-phenylindole (DAPI) was added to a final concentration of 0.1 μg/ml. The stained eggs were transferred to a slide, covered with a coverslip and sealed with clear nail polish. Eggs were viewed with an Olympus IX70 inverted microscope using a 40× (NA 0.75) objective lens and settings for both Nomarski (Differential Interference Contrast) and fluorescent DAPI imaging. Squash preparations to count nuclei of developing embryos were prepared as reported previously .
Developing embryos (n = 242) were observed at room temperature (22+/-1 °C) by Nomarski (Differential Interference Contrast microscopy) or bright field optics using a Nikon inverted microscope with a 40× oil objective (NA 1.3). A motorized stage controller and automatic shutter were incorporated in the system to create a 4D-imaging capacity. The essential software to control both the stage and the shutter was written in JAVA and integrated in Lasersharp 2000 v5.2 software (BioRad, Hercules, CA). Images (800 × 600 pixels; 468 kb) of developing embryos were taken every 15 or 30 min in 20 different focal planes during the first 2 weeks and every 12 h during the remaining period using a CoolSNAP HQ CCD camera (Photometrics, Tucson, AZ). To create time-lapse videos the optical section of interest for each time point was manually selected. Subsequent imaging procedures were performed using ImageJ version 1.371 (available via http://rsb.info.nih.gov/ij/). Twenty embryos were monitored for viability after finishing recordings and all hatched in to J2 nematodes.
A total of 242 embryos were imaged to generate 242 videos of various lengths. The longest videos, which were derived from 6 embryos followed for 12.5 days, resulted in over 500 time points (frames). We followed about 20 embryos after imaging and they all hatch, which suggest that the treatment during imaging does not affect their development.
Early cell lineage analysis
The early lineage was reconstructed using the Simi Biocell software (Simi Gmbh, D-85705 Unterschleissheim, Germany) based on three embryos, each developed up to the final embryonic stage . Specific events were studied in additional embryos (n = 242) for confirmation. The terminology introduced by  for C. elegans development was followed to denote the different cells and stages during embryonic development. Herein “a” and “p” stand for anterior and posterior respectively, and “l” and “r” stand for left and right respectively.
Western blotting was used to test the ability of the following three primary antibodies (obtained from the Hybridoma Bank at the University of Iowa) to cross-react with M. incognita proteins: Tetrahymena anti-α-tubulin (12G10; ), C. elegans anti-P-granule (K76, and OICD14; [20, 21]), and C. elegans anti-Par-3 (P4A1; ). Only the Tetrahymena anti-α-tubulin antibody showed cross reactivity (data not shown) and was used to conduct the immunohistochemistry experiments described here; the C. elegans antibodies tested did not react with M. incognita proteins.
Standard protocols developed by Susan Strome (University of California, Santa Cruz) were adapted for use with the nematode embryos. For each slide, freshly extracted M. incognita embryos were diluted to a concentration of 50 embryos/μl, and placed on a polylysine slide. A coverslip was placed on top of the embryos, and the slide was immediately transferred to dry ice. The coverslip was pressed down during the incubation on dry ice to ensure that the embryos adhered to the slide. The slide remained on dry ice until the embryos froze, after which the coverslip was quickly removed with a razor blade and the slide transferred to a coplin jar containing 100 % methanol at -20 °C, incubated for 10–15 min, then immediately transferred to a second coplin jar containing acetone at -20 °C, incubated for an additional 10–15 min. The slide was subsequently washed three times for, 5 min each, in a coplin jar containing freshly prepared phosphate buffered saline (PBS) with 0.1 % Tween-20, at room temperature. The slide was allowed to dry completely, and a perimeter denoted with a hydrophobic pen (Immedge™, Vector Laboratories, Inc., Burlingame, CA) around the region containing the embryos. The slide was incubated overnight at 4 °C with primary antibodies diluted 1:10 in PBS with 0.1 % Tween-20 and 0.1 % bovine serum albumin (BSA). The following day, the slide was washed with PBS with 0.1 % Tween-20 three times for five min each, and then incubated with FITC-conjugated anti-mouse secondary antibody, diluted 1:100 in PBS with 0.1 % Tween-20 and 0.1 % BSA, for two h at room temperature. The slide was again washed three times, 5 min each, with PBS with 0.1 % Tween-20 and a propidium iodide (PI) solution (0.2 μg/ml in PBS, 70 % glycerol) was added to the embryos and sealed with a coverslip and petroleum jelly or clear nail polish. These embryo containing slides were viewed and documented with an Olympus FluoView 300 confocal microscope.
During maturation of the egg in the female, the cellular mass filled the egg completely. Prior to the initiation of embryonic development, the cell condenses and the lipoid membrane pulls away from the eggshell, leaving a perivitelline space at both poles of the egg. Centrally located in the granular cytoplasm, the nucleus was observable as a large distinct sphere. The developmental stage of the egg when deposited in the egg mass was the single- or two-cell embryonic stage. Only in rare cases further developmental stages could be detected in the reproductive system of older females. These may be the last eggs that are not laid by an exhausted female .
Early cell lineage analysis
To study the early development of living embryos in detail, 4D-video recordings were made (Fig. 3; Additional file 1); in particular three recordings each representing the first 12.5 days of development were used to construct the early cell lineage. Prior to the first cell division, there was high cytoplasmic activity and extensive nuclear movement (Figs. 4 and 5). These events were accompanied with dramatic contractions and membrane ruffling of the single cell. During this period multiple pseudocleavage furrows could be seen, which distorted the shape of the cell (Fig. 4). The duration of this period was extremely variable, lasting less than one hour in some eggs to several days in others. Unfortunately, and since the exact moment when the eggs are laid is unknown, it is not possible to accurately determine if there is a pattern as to how long it takes for the eggs to enter the first cell division. Having said this, 50 eggs (at the one cell stage) were mounted and imaged, and the time between mounting and the first cell division was estimated. From this survey it appears that eggs can stay for long periods of time without undergoing development (up to 60 h). Once development started, all embryos took a similar time to complete early development, although none of these embryos was followed to determine if they would hatch to produce J2s.
Sequence of the first divisions
Cell number after division
Cell number after division
In M. incognita gastrulation started around 131 h, in the 26-cell stage (Fig. 3e). Like in C. elegans, the two daughter cells of the E cell were translocated from the ventral side to the center of the embryo. The posterior endodermal precursor cell Ep first migrated inwards between the four MS granddaughters MSaa, MSap, MSpa and MSpp and was then followed by its anterior sister cell, after which they divided left-right. Tracking individual cells beyond this stage (48 cells) was difficult because heavy granulation of the cells obscured details. As a result, cell divisions were not followed in detail beyond this time point, but some specific developmental stages could be distinguished.
Cell differentiation after gastrulation resulted in the formation of large endodermal cells surrounded by smaller ectodermal cells (Fig. 3g). Both layers developed further and resulted in the formation of two zones of dissimilar density within the embryo, a light anterior area packed with cells (as demonstrated with DAPI staining; data not shown) and a dark posterior zone (Fig. 3i). After the spheroid embryo started to elongate, it was squeezed against the eggshell and forced to bend, forming a worm-like two-fold embryo (Fig. 3j-k). Compared to the total early developmental time (approximately 21 days), elongation of the embryo took place quite rapidly (approximately 3 days) and resulted in a first stage juvenile, coiled three to four times within the eggshell. As soon as the nematode started to elongate, it moved frequently and vigorously, indicating that body muscles had started to function. These movements continued until the hatching of the J2 approximately 21 days after the first cell division.
Cytoskeleton organization at the single cell stage and during early cell divisions
Microtubules organization in opposite sides of the nucleus reached a maximum at the time when a line going through the two MTOC was parallel to the longer axis of the cell (Fig. 8b). After the first cell division (as seen in most of the studied lineages) the A-P axis was determined. In those eggs where the division was unequal, the smaller cell was the posterior (P1; posterior lineage) cell and the larger was the anterior (AB) cell (Fig. 8c). In the posterior cell, and prior to the next cell division, the two MTOC were localized along the A-P axis and the cell’s DNA could be clearly seen between them (Fig. 8d; in this image the P1 cell is at metaphase). Similarly, and prior to the cell division of the anterior AB cell, the MTOCs were localized parallel to the A-P axis (Fig. 8e; in this image the AB cell is at anaphase). After generation of the four blastomere stages (ABa, Abp, EMS and P2), the cells adjusted their positions so that the ABa and EMS cells were touching each other on what will become the ventral surface of the embryo, and the P2 cell divided to generate C and P3; at this stage EMS began to divide and the MTOCs organized parallel to the A-P axis (Fig. 8f).
M. incognita eggs are characterized by high cytoplasmic activity and extensive nuclear movement
The ellipsoid eggs of the root-knot nematode, M. incognita, lack overall uniformity. This observation has been recorded for the eggs of several nematodes [28, 29] and it was even suggested that intra-specific differences might be as great as inter-specific variations (described in ). Individual eggs were followed by 4D-microscopy during the developmental process to study successive cell divisions.
Two events that show similarity between C. elegans and M. incognita, prior to the first cell division, are cytoplasmic ruffling and nuclear migration. In C. elegans, adjustments are made to the newly fertilized embryo; cortical proteins, other cytoplasmic material and organelles are moved to defined regions by the cytoskeleton. These movements are reflected morphologically as a series of cytoplasmic ingressions and invaginations in the cell membrane [11, 30]. Proteins and ribonucleoproteins, such as the PAR proteins and P-granules, respectively, are repositioned in the newly fertilized zygote . Similarly, cytoplasmic ruffling takes place in the single-celled embryo of M. incognita, suggesting that there is a need to organize the embryo prior to the first cell division. However, it is unclear whether the redistribution of cytoplasmic materials and/or the unequal size of the daughter cells are responsible for determination of the A-P axis. It is also unclear whether a PAR-3-like mechanism is involved, since the antibodies tested did not return cross-reactivity. Further testing is required to demonstrate whether or not a PAR-3 like mechanism exists, and whether it is involved in the development of M. incognita. It is also possible that other PAR proteins, such as PAR-1, −2, −4 or −6, are involved; each of these proteins are known to be evolutionarily conserved in many organisms and function in cell polarization during development .
Multiple pathways initiate embryogenesis across many different species of nematodes. For instance, electrical pulses, changes in calcium levels or physical disruption of the oocyte’s membrane (poking) can initiate embryogenesis in some species [33, 34]. In C. elegans, an enucleated sperm was shown to initiate embryogenesis, activating the oocyte and leading to the first cell division . Few studies have described the embryonic development in parthenogenetic nematode species, and the nature of the signals that trigger embryonic development are not yet clear. Lahl et al.  describe the development of three free living parthenogenetic nematodes: Acrobeloides nanus, Diploscapter coronatus and Plectus sp. In these species, the eggs were deposited as single-celled embryos into the environment, and there was no evidence for oocyte organization prior to the first cell division. Furthermore, the single-celled eggs of M. incognita initially have a soft chorion before they are deposited into the external environment; only then do the chorionic shells harden. The vulva of the female compresses the egg when it is deposited into the gel matrix (female extracellular secretions, which will hold the eggs/embryos) and it is possible that this physical action is the trigger to initiate development. In our study with M. incognita, we only observed single-celled embryos transferred through the reproductive tract of the female, which are deposited as single- or two celled embryos into the egg mass. The eggs seem to be dormant for long, variable intervals (in one recording the egg was dormant for 4.5 days; see Additional file 4), and then a currently unidentified trigger initiates embryogenesis. As in other animals, it is possible that nuclear movement defines where the cleavage furrow will form, and whether the first cell division will be asymmetrical . Nuclear positioning in C. elegans is a PAR-3-dependent process, but it is unclear whether a similar mechanism is involved in M. incognita.
We identified the presence of polymerized α-tubulin in developing M. incognita embryos, using antibodies that have been shown to react with the α-tubulin of several diverse species . Using time-lapse microscopy and immunohistochemistry, we observed both cytosol containing a high density of smaller particles and a tightly knit microtubules in one highly compacted region of the single-celled embryo. Prior to the first cell division, the nucleus migrated away from the high density, small-granule region into the low density large-granule region, where the first asymmetrical cell division took place. It is possible that the combination of high cytoplasmic activity, extensive nuclear movement and the contractions and membrane ruffling of the single cell are caused by the constant reorganization of components of the cytoskeleton, for example contractility of the actin cytoskeleton as it is the case in C. elegans [7, 37]. Since the polarity cue in M. incognita is unknown (because no sperm contribution), it is tempting to speculate a self-activation mechanism of the actin cytoskeleton. The first cell division, generating the AB and P1 cell, are always located in the low-density region of the single cell. Hence, it is tempting to speculate that the cytoplasmic density gradient and asymmetrical distribution of the cytoskeleton induced a first asymmetric division. Our results of cytoplasmic rearrangements prior to the first cell division in M. incognita seem to contradict earlier findings , and we have no explanation for this contradiction.
M. incognita has a slow developmental tempo
M. incognita showed an extremely slow embryonic development compared to C. elegans; the development was at least 35 times slower (development from first cell cleavage to hatching almost 500 h in M. incognita and 14 h in C. elegans at room temperature). For early embryogenic events this difference is even grater: the time between division of the P0 and the division of the somatic founder cell E is less than 1 h for C. elegans (47 min), but approximately 114 h for M. incognita. Similarly, the completion of a cell cycle during early embryogenesis required several hours, whereas in early developmental stages C. elegans cells divide approximately every 10 min.
The optimal temperature for development of M. incognita is 28 °C, and corresponds to the geographical distribution of this nematode in subtropical regions. At this temperature it takes approximately 3 weeks to complete one life cycle. Higher temperatures (above 30 °C) have a devastating effect on the survival rate and lower temperatures result in an extension of the life cycle (discussed in Tyler, 1933b ). However, the high optimal temperature (28 °C) had a disturbing effect on the agar pad and resulted in out of focus drifts during imaging; this drift could be a consequence of the objective lens contacting the cover slip and the continuous moving of the slide from embryo to embryo causing a change in pressure and resulting in the out of focus drift. For this reason we chose to follow the development at room temperature (22 °C ± 1 °C). The use of this suboptimal temperature could partly explain the slow embryonic development, but it is unlikely that it affected the sequence of cell divisions [40, 41]. Vangestel et al.  studied the influence of temperature (15 °C, 20 °C and 25 °C) on early embryonic development in Pristionchus pacificus (Neodiplogasteridae) and found that besides developmental tempo, all the other examined parameters were found to be similar at different temperatures (division sequence, time of establishment of the P4 cell, gastrulation and cell-cell contacts). The slow development of M. incognita appears to be typical for tylenchids since similar times have been described for Neodolichodorus rostrulatus [9–10 days; ], Scutellonema cavenessi [10–11 days; ], Pratylenchus penetrans and P. zeae [10 days; ], and Hoplolaimus columbus [12 days; ]. Slowly developing nematodes are also found in clade 1 (Fig. 9) of the phylogeny of Holterman et al. . In the marine nematodes Enoplus brevis and Pontonema vulgare the time until hatching is 16–20 days at 25 °C  and 30 days at 16 °C , respectively. These enoplids of clade 1 (clades as defined in ) are found in a stable marine habitat. Schierenberg formulated that this slow development was probably necessary to preserve aspects of regulative development. Associated with the colonization of freshwater and terrestrial habitats, nematodes possibly needed to respond to more rapidly changing environments and thus nematodes that developed faster, or nematodes that were more tolerant to changing environmental conditions, had a selective advantage . Besides these slowly developing nematodes from clade 1 and M. incognita in clade 12, which shows a very slow development, more slowly developing nematodes were also found in other clades, e.g. Parascaris equorum (clade 8) having an embryonic development of 1–2 weeks at room temperature (28 °C). Hence, it appears that the speed of development has changed independently in several taxa.
The embryonic development shows similarities to C. elegans, but also features typical for Cephalobomorpha
M. incognita has a fixed cleavage pattern and the development is described as synchronous , similar to C. elegans. The cell-cell contacts MS-ABara and MS-alp, which induce pharyngeal potential in those cells in the C. elegans embryo [25–27], were also identified in M. incognita. As in C. elegans, only one member of two bilateral homologs of blastomeres came into contact with the signaling blastomere MS. Whether signaling from MS effectively results in left-right asymmetries through the Notch pathway, as in C. elegans, remains to be determined (reviewed by .
Essential differences from C. elegans exist in the timing of germline divisions. While in C. elegans the primordial germ cell P4 is present in the 24 cell stage, P4 is already present in the 9 cell stage in M. incognita. This is in contrast to the previous findings of Dolinski et al. , who reported a simultaneous cleaving for the EMS and P3 cell. Cephalobidae, however, are characterized by the division sequence P1-P2-AB-P3. Here, the P4 cell is established already at the 6-cell stage. Skiba and Schierenberg  stated that in these slow developing embryos the germline is separated relatively early, to preserve germline quality. Examining the configuration of the posterior cells, we observed a variable pattern. In C. elegans the germline daughter cells P0 and P1 are always positioned posterior to their somatic sister cell, while after the division of P2, the germline cell P3 will be in an anterior position and its somatic sister C in a posterior position. This phenomenon is described as “reversal of cleavage polarity” [PR; ]. In M. incognita, the position of the germline cell P3 was not determined and can be both posterior and anterior to its somatic C cell. This variable configuration is in contrast to observations of Goldstein et al.,  who stated an absence of polarity reversal for M. incognita. The variations in spatial patterns are not unique to M. incognita, but were first described for Cephalobus sp. (later referred to as Acobeloides nanus) by Skiba & Schierenberg . In this species the alternative orientation of the cleavage spindle in AB results in 2 different arrangements of blastomeres in the 5-cell stage [50, 52]. In M. incognita however, this did not depend on the orientation of AB’s cleavage spindle as the two AB cells were already present when P2 divided. This variable spatial pattern was also observed in four other members of the Cephalobomorpha, and is possibly a synapomorphy for Tylenchomorpha and Cephalobomorpha . Nevertheless, the variable configuration of these cells is also found in other clades. Using experimental interference, Laugsch and Schierenberg  found variable configurations in three Rhabditis species and Lahl et al.  mentioned variable configurations for Diploscapter coronatus. In these nematode embryos, subsequent cellular migrations restore contact between the germline and the endodermal precursors, leading to the C. elegans spatial arrangement (P4-D-C) before the onset of gastrulation. Skiba and Schierenberg  suggested that this configuration is required for further normal development. This phenomenon is also observed in other species: in Drosophila, Xenopus, chick and mouse the primordial germ cells associate with the developing gut, from which they migrate to the gonads during organogenesis .
The work presented here on the early development of the apomictic M. incognita reveals some common features with the early development of the sexually reproducing C. elegans such as cytoplasmic rearrangements prior to the first cell division, a fixed cleavage pattern and early synchronous development. However, major differences also exist such as the timing of germline divisions (P4 being present in the 9-cell stage), the developmental tempo (at least 35 times slower in C. elegans), and the variable configuration of the posterior cells.
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Availability of data and material
The data supporting the conclusions of this article is included within the article and its additional file(s).
BV received a postdoctoral grant from Ghent University. The work conducted by SK for this publication was part of his MS thesis work at Fresno State. The authors would like to thank Geert Meesen for software development, Paul Crosbie and Glenda W. Polack for critically reading the manuscript, Paul Sternberg for comments and suggestions, and John Constable and two anonymous reviewers for insightful comments.
This work was funded by grants from the California Agricultural Research Initiative (grant #: ARI/CATI/Calderón-Urrea/Cell Death/03-2-006-31), the College of Science and Mathematics at Fresno State, the California State University Program for Education and Research in Biotechnology (CSUPERB), and the RIMI Facility at Fresno State (development of this facility was funded by NIH-NIMHD grant “Research Infrastructure for Minority Institutions P20MD002732”).
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