Fig. 2
From: MiR-21 is required for efficient kidney regeneration in fish
MiR-21 is up-regulated after renal injury and can be blocked specifically using LNA-antimiR-21. a Expression of miR-21, miR-30a, miR-194, miR-200a after induction of renal injury. MiRNA expression was measured using qRT-PCR. n = 4 fish/time point b Sequence analysis of miR-21 in different species. c In situ hybridization to show localization of miR-21 in fish kidneys at different time points after renal injury. For miR-21 detection an LNA-probe was used on kidney cryosections. Scale bar: 50 μm d EdU incorporation assay in combination with in situ hybridization to label proliferating cells and miR-21 expression. e Analysis of miR-21 expression after injection of antimiR-21 in two different concentrations and in four different tissues. n = 4 fish/time point f Time course of antimiR-21 stability in the kidney. g and h MiR-30 and miR-194 expression was measured by qRT-PCR in control and antimiR-21 treated kidneys. n = 4 fish/time point, one-way ANOVA or student’s t-test was used: *p < 0.05, **p < 0.01, ***p < 0.001