Identification of known and novel pancreas genes expressed downstream of Nkx2.2 during development

Table 3 Downregulated genes in Nkx2.2^-/- versus Nkx2.2^+/+ pancreata at e12.5.

Genbank Accession Number	*Gene Symbol*	Gene Name	e13.5 Fold Change
Secreted Factors
NM_008387	Ins2	INSULIN II	-3.4
NM_170593	Disp2	DISPATCHED HOMOLOG 2 (DROSOPHILA)	-1.8
NM_009523	Wnt4	WINGLESS-RELATED MMTV INTEGRATION SITE 4	-1.6
Transcription Factors
NM_008393	Irx3	IROQUOIS RELATED HOMEOBOX 3 (DROSOPHILA)	-2.6
NM_008665	Myt1	MYELIN TRANSCRIPTION FACTOR 1	-2.4
NM_007960	Etv1	ETS VARIANT GENE 1	-1.8
NM_021459	Isl1	ISLET-1 TRANSCRIPTION FACTOR, LIM/HOMEODOMAIN	-1.5
Transmembrane Proteins
NM_001014761	Scn2b	SODIUM CHANNEL, VOLTAGE-GATED, TYPE II, BETA	-2.0
NM_024226	Rtn4	RETICULON 4	-1.9
Transporters
NM_007514	Slc7a2	SOLUTE CARRIER FAMILY 7 (CATIONIC AMINO ACID TRANSPORTER, Y+ SYSTEM), MEMBER 2	-2.1
NM_028924	Mtac2d1	MEMBRANE TARGETING (TANDEM) C2 DOMAIN CONTAINING 1	-1.6
Signaling
NM_007832	Dck	DEOXYCYTIDINE KINASE	-2.3
Metabolism
NM_007748	Cox6a1	CYTOCHROME C OXIDASE, SUBUNIT VI A, POLYPEPTIDE 1	-2.0
Chromatin
NM_175074	Hmgn3	HIGH MOBILITY GROUP NUCLEOSOMAL BINDING DOMAIN 3	-1.7
Other
NM_152800	Tor2a	TORSIN FAMILY 2, MEMBER A	-2.4

15 additional genes were downregulated in Nkx2.2^-/- pancreata at e13.5. Genes were identified using PancChip 6.1 microarray analysis and a fold change of 1.5 was used as a cutoff for determining expression changes. Genes were categorized based on function determined by gene ontology (GO) term associations.

ISSN: 1471-213X