Identification of known and novel pancreas genes expressed downstream of Nkx2.2 during development

Table 2 Upregulated genes in Nkx2.2^-/- versus Nkx2.2^+/+ pancreata at e12.5 and e13.5.

Genbank Accession Number	Gene Symbol	Gene Name	e12.5 Fold Change
Secreted Factors
NM_021488	Ghrl	GHRELIN	6.5
NM_010491	Iapp	ISLET AMYLOID POLYPEPTIDE	5.2
NM_025684	Nepn	RIKEN CDNA 5730521E12 GENE	3.1
Transmembrane Proteins
NM_145539	Tm4sf4	TRANSMEMBRANE 4 SUPERFAMILY MEMBER 4	4.5
NM_021286	Sez6	SEIZURE RELATED GENE 6	2.4
NM_030701	Gpr109a	G PROTEIN-COUPLED RECEPTOR 109A	1.9
NM_008150	Gpc4	GLYPICAN 4	1.8
NM_007417	Adra2a	ADRENERGIC RECEPTOR, ALPHA 2A	1.7
Peptidases
NM_033612	Ela1	ELASTASE 1, PANCREATIC	1.9
Metabolism
NM_011107	Pla2g1b	PHOSPHOLIPASE A2, GROUP IB, PANCREAS	2.7
NM_007981	Acsl1	ACYL-COA SYNTHETASE LONG-CHAIN FAMILY MEMBER 1	2.0
NM_007482	Arg1	ARGINASE 1, LIVER	1.8
Chromatin
NM_011417	Smarca4	SWI/SNF RELATED, MATRIX ASSOCIATED, ACTIN DEPENDENT REGULATOR OF CHROMATIN, SUBFAMILY A, MEMBER 4	3.6
Other
CV677510	IMAGE = 6437263	Unknown	1.8
NM_145616	Lrrc49	LEUCINE RICH REPEAT CONTAINING 49	1.7
NM_173405	6530401C20Rik	RIKEN CDNA 6530401C20 GENE	1.7

16 genes were upregulated in Nkx2.2^-/- pancreata at e12.5 and e13.5. Genes were identified using PancChip 6.1 microarray analysis and a fold change of 1.5 was used as a cutoff for determining expression changes. Genes were categorized based on function determined by gene ontology (GO) term associations.

ISSN: 1471-213X