Figure 4

Galanin-like immunoreactivity in regenerating radial nerves. Longitudinal tissue sections of regenerating radial nerve cords at (A) 6, (B) 12 and (C) 28 days post injury (dpi) were labeled with anti-galanin (green) and counterstained with Hoescht (red) to label cell nuclei. (A) Galanin immunoreactivity disappears from the injury edge of the cord (asterisk) and the proximal stump acquires a punctuated labeling at 6 dpi. (B) At 12 dpi, the immunoreactive fibers have crossed the injury gap and labeling appears more homogeneous. (C) At 28 dpi labeling is similar to that in the uninjured cord (not shown). EN-ectoneural component, HN-hyponeural component, RNC-radial nerve cord. X's denote the injury site. Bar = 300 μm.