Open Access

Lbx2regulates formation of myofibrils

BMC Developmental Biology20099:13

https://doi.org/10.1186/1471-213X-9-13

Received: 18 August 2008

Accepted: 12 February 2009

Published: 12 February 2009

Abstract

Background

Skeletal muscle differentiation requires assembly of contractile proteins into organized myofibrils. The Drosophila ladybird homeobox gene (lad) functions in founder cells of the segmental border muscle to promote myoblast fusion and muscle shaping. Tetrapods have two homologous genes (Lbx). Lbx1 functions in migration and/or proliferation of hypaxial myoblasts, whereas the function of Lbx2 is poorly understood.

Results

To elucidate the role of Lbx in vertebrate myogenesis, we examined Lbx function in zebrafish. Zebrafish lbx2 transcripts appear in newly formed paraxial mesoderm and become restricted to adaxial cells, precursors of slow muscle. Slow muscles lose lbx2 expression as they differentiate, while a subset of differentiating fast muscle cells transiently expresses lbx2. Fin and hyoid muscle express lbx2 later. In contrast, lbx1b expression first appears lateral to the somites at late segmentation stages and is later restricted to fin muscle. Morpholino knockdown of Lbx1b and Lbx2 suppresses hypaxial muscle development. Moreover, knockdown of Lbx2 results in malformation of muscle fibers and reduced fusion of fast precursors, although no obvious effects on induction or specification are observed. Expression of myofilament genes, including actin and myosin, requires the engrailed repressor domain of Lbx2.

Conclusion

Our results elucidate a new function of Lbx2 as a regulator of myofibril formation.

Background

Skeletal muscle progenitors are specified as a population of multipotent mesodermal cells. These cells subsequently commit to a muscle fate and differentiate into muscle fibers by cell fusion and assembly of contractile myofibrils composed of myosin thick filaments and actin thin filaments. Although the molecular mechanisms that regulate induction of muscle progenitors, commitment of these cells to the muscle lineage, and myoblast fusion are well characterized [15], relatively less is known about the mechanisms that regulate the formation of myofibrils [6, 7].

The ladybird (Lbx) protein is a member of the homeobox transcription factor family, characterized by an N-terminal engrailed repressor domain. In Drosophila, lbx is expressed by progenitor cells, founder cells, and syncytial precursors of a larval somatic muscle, the segmental border muscle (SBM, muscle 8) [8, 9]. Embryos lacking lbx function have missing muscle fibers, unfused myoblasts, or abnormally shaped cells in the SBM. Ectopic lbx expression leads to the formation of enlarged or duplicated SBMs [8]. Recent studies showed that Lbx is also required for establishment of morphological, ultrastructural, and functional properties of leg muscles in Drosophila [10]. Thus, the lbx gene functions in fusion of myoblasts, development of muscle fibers, and the establishment of muscle morphology in Drosophila.

In mouse, two Lbx genes, Lbx1 and Lbx2, have also been identified [11]. Lbx1 is expressed in a subset of hypaxial myoblasts that migrate into the limbs, tongue, and diaphragm. Lbx2 is expressed in the central nervous system, in neural crest derived structures such as dorsal root ganglia and other parts of the peripheral nervous system, and in the urogenital system [11]. Loss of Lbx1 function results in lack of specific limb musculature, attributed to migration defects [12, 13]. Although, Lbx2 knock out mice develop relatively normally, one study suggested that Lbx2 functions in neural-derived tissues under the regulation of Pax3 [14]. Lbx1 has also been studied in Xenopus, where it represses myod expression and promotes myoblast proliferation before the onset of terminal differentiation [15]. Thus in both mammals and frogs, Lbx1 is thought to function in migrating myoblasts, whereas functions of Lbx2 are still unclear [14].

Many aspects of the molecular pathway that regulates myogenesis are conserved in Drosophila and vertebrates [5, 16]. However, because studies of vertebrate Lbx have focused mainly on migration or proliferation of myoblasts in hypaxial myogenesis, little is known about whether Lbx functions in other aspects of muscle development that have been characterized in Drosophila, such as fusion of myoblasts, formation of fibers, or the establishment of muscle shapes. To study these potential roles of Lbx in vertebrates, we examined lbx gene function in zebrafish. Zebrafish axial skeletal muscles contain four fiber types; slow muscle, muscle pioneers, fast muscle, and medial fast fibers. Slow muscle cells are located superficially just under the skin, with fast muscle cells located deeper [1]. Muscle pioneers are located near the horizontal myoseptum that separates dorsal and ventral parts of the myotomes, and medial fast fibers are also located medially in the somite. We previously showed that adaxial cells located adjacent to the notochord are precursors of slow muscle cells and muscle pioneers [17, 18]. Adaxial cells are specified to form slow or muscle pioneers by Hh signaling from the notochord [19], Then, slow muscle precursors migrate to the lateral surface of the somite [18]. After the somite forms, non-adaxial muscle precursors in the segmental plate differentiate into fast muscle cells. During this period, both slow and fast muscle precursors are dynamically rearranged in the somite [20, 21].

The zebrafish genome contains three lbx genes [22].lbx2, previously named lbx1, is expressed in the ventral region of the somite, in the hindbrain, and in the fin bud [23]. lbx1a is expressed in the nervous system and fin bud [24]. Analysis of lbx1b expression in zebrafish has not been previously reported[22].

Here, we show that zebrafish lbx2 expression first appears at late gastrula stages (70%–80% epiboly) in cells near the margin that later form head muscle and pronephros. Subsequently, lbx2 expression is present in the paraxial mesoderm and adaxial cells, which are the precursors of slow muscle and muscle pioneers [1, 18]. Although a subset of adaxial cells migrates into the somite during segmentation stages[18], lbx2 is not detected in slow muscle cells during or after their migration. As development proceeds to late segmentation stages, a subset of fast muscle cells in both dorsal and ventral regions of the somite turns on lbx2 expression that subsequently disappears by the second day of development. Thus, myoblasts in both the slow and fast muscle lineages express lbx2 transiently. As development proceeds to the end of the second day, lbx2 expression is detected in fin bud and hyoid, similar to Xenopus lbx1 expression. In contrast to lbx2, lbx1b expression is not detected during gastrulation, but first appears in the hindbrain and caudal regions of the neural tube around the 5-somite stage. As development proceeds, a subset of cells lateral to the somites (presumptive fin bud) begins to express lbx1b, whereas somite cells never express lbx1b. Later, lbx1b expression is present in the fin buds. Thus, myoblasts only in fin lineages express lbx1b. Morpholino analysis shows that both Lbx1b and Lbx2 function in hypaxial myogenesis. In addition, although Lbx2 is not required for induction of myogenesis or specification of zebrafish skeletal muscle cell fates, Lbx2 is required for normal fusion of fast muscle precursors, as in Drosophila. Lbx2 also plays an important role in the formation of myofibrils by regulating expression of filament genes, such as myosin and actin. We further demonstrate that the engrailed repressor domain of the Lbx2 protein is required for this induction of filament genes. Thus, although previous studies in vertebrates have suggested that Lbx1 functions in proliferation and migration of muscle precursors, our results demonstrate that vertebrate Lbx2 also plays an important role as a regulator of muscle cell differentiation.

Results

Slow and fast muscle precursors transiently express lbx2mRNA

To study the function of lbx genes in vertebrate skeletal muscle development, we examined expression of lbx1b and lbx2 in zebrafish. Previous analysis of lbx1a [24] showed that it is not expressed in the somites, so we excluded it from this study. lbx2 expression becomes detectable by the 70%–80% epiboly stage (Fig. 1A). Double labeling with no tail (ntl), which marks the blastoderm margin, reveals that lbx2 first appears adjacent to the margin (Fig. 1H) in the region that later contributes to head muscle and pronephros [25]. By the end of bud stage, lbx2 expression appears in paraxial mesoderm and adaxial cells (Fig. 1C, J, brackets), precursors of slow muscle cells and muscle pioneers [18]. lbx2 expression is not detected in the somites (Fig. 1D, K, rostral region), even though a subset of adaxial cells, the slow muscle precursors, migrates through the somites during segmentation stages [18, 20, 21].
Figure 1

Muscle precursors express lbx1b and lbx2. A-L: Muscle precursors transiently express lbx2 . A-G, L: Expression of lbx2 at 70%-epiboly (A), 90%-epiboly (B), bud (C), segmentation (D), 24 hpf (E, L), 48 hfp (F, G). Whole-mounts. H-K: lbx2 (blue) and ntl (red) expression at 70%-epiboly (H), 90%-epiboly (I), bud (J), and segmentation (K). Flat-mounts. L: Transverse section, 24 hpf embryo, F59 (green) and lbx2 mRNA (blue). (A, H) lbx2 mRNA appears at 70%-epiboly adjacent to ntl expressing cells in blastoderm margin. lbx2 expression in paraxial mesoderm by end of gastrulation (B-C, I,). lbx2 expression later restricted to adaxial cells (J-K, brackets). (L) Subset of fast muscle cells expresses lbx2 in epaxial (black arrow) and hypaxial (black arrowhead) domains. F59 and lbx2 labeling shows differentiated slow muscle cells lose lbx2 expression (L, white arrowhead). (F) lbx2 expression in trunk disappears by 48 hpf (F, bracket). lbx2 expression in fin primordia (F-G, black arrowheads), hindbrain (F-G, white arrowhead), and hyoid (F, white arrow). M: Diagram of zebrafish muscle. Adaxial cells (K, bracket) migrate superficially and differentiate into slow muscle fibers (green). Then, fast (magenta) and medial fast fibers (red) differentiate. N-R: 5-somite (N), 18-somite (O), 24 hpf (P-Q) and 48 hpf (R). (Q) Higher magnification of P. lbx1b mRNA appears at 5-somite stage in neural tube (N), along rostral-caudal axis (O, arrow), then lateral to somites (P-Q), and later in fin (R). (A-D, N-O) Dorsal views, rostral towards the top; (E-F, P) lateral views, rostral toward the left, dorsal toward the top; (H-K) rostral toward the top; (L) dorsal toward the top; (G, R) dorsal views, rostral toward the left. Scale bars: (A-D, N-O, Q) 200 μm, (E-G, P, R) 100 μm, (H-L) 50 μm.

To learn whether differentiated slow muscle cells express lbx2, we double-labeled embryos for lbx2 mRNA and with the F59 antibody, a marker of slow muscle [18]. We find that differentiated slow muscle cells do not express lbx2 (Fig. 1L, white arrowhead). Instead, lbx2 expression appears in the fast muscle domain by late segmentation stages (Fig. 1E, L). Interestingly, we find that both dorsal and ventral fast muscle cells express lbx2 (Fig. 1E, L, black arrow and arrowhead respectively), whereas previous studies reported that lbx2 expression is restricted to ventral regions of the somite [23]. By 48 hours post-fertilization (hpf), lbx2 disappears from the trunk (Fig. 1F, bracket) and appears in fin muscles as previously described (Fig. 1F, G, black arrowheads)[13, 23]. lbx2 is also expressed in a dorsal intermediate region of the neural tube and hindbrain (Fig. 1F, G, white arrowhead)[15, 23] and in the hyoid (Fig. 1F white arrow)[15, 23].

Unlike lbx2, lbx1b expression is not detected during gastrulation. lbx1b expression first appears within a dorsal intermediate region of the neural tube by the 5-somite stage (Fig. 1N, arrow), and this expression extends along the rostral caudal axis by late segmentation stages (Fig. 1O, arrow). As development proceeds, a group of cells lateral to the somites (presumptive fin bud) begins to express lbx1b (Fig, 1P, Q, arrowhead), whereas muscle precursors in the somites do not express lbx1b. Subsequently, lbx1b expression is detected in the fin bud (Fig. 1R, arrowheads). Unlike lbx2, the hyoid does not express lbx1b (Fig. 1R). Thus, during myogenesis, lbx2 expression appears in paraxial mesoderm, precursors of slow and fast muscles of the somite, fin bud, and hyoid, whereas lbx1b is detected only in myoblast lineages of the fin.

Conserved function of lbxin hypaxial muscle development

To examine Lbx function in myogenesis, we knocked down Lbx2 or Lbx1b activity using specific splice blocking morpholino oligonucleotides (MOs). To confirm the specificity of the MOs, we performed reverse transcriptase PCR on mRNA from MO injected embryos. We find that injection of lbx2 or lbx1b splice donor MO results in production of aberrantly spliced lbx2 or lbx1b transcripts, respectively (Fig. 2A), indicating that splicing of lbx2 and lbx1b is blocked by the specific lbx splice donor MOs.
Figure 2

Lbx2 and Lbx1b function in hypaxial muscle development. A: Splice donor MOs against lbx2 or lbx1b inhibit correct splicing of lbx2 or lbx1b, respectively. RT-PCR was performed using bud stage (lbx2) or segmentation stage (lbx1b) embryos. Spliced bands and unspliced bands in the lbx-splice donor-MO lane indicate aberrantly spliced message increases and correctly spliced message decreases. Arrows indicate primers. En: Engrailed domain, HD: homeodomain. Arrows indicate specific primers for amplification of correctly spliced or unspliced lbx genes. B-G: Expression of myod in control (ctrl) embryos (B, C), lbx2-MO injected embryos (D, E). lbx1b-MO injected embryos (F, G). The white arrowhead indicates the sternohyoideus primordium (B) and the white arrows indicate fin muscle precursors. myod expression in fin bud is suppressed by lbx2-MO or lbx1b-MO (B: 100%, n = 36; D: 12%, n = 56; F: 16%, n = 24). H-M: Expression of fgf8 in ectodermal cells of the fin bud. Control embryos (H, I, 100%, n = 12), lbx2-MO injected embryos (J-K, 100%, n = 12), lbx1b-MO injected embryos (L-M, 100%, n = 15). (B, D, F, H, J, L) Dorsal views, rostral towards the left, (C, E, G. I, K, M) lateral views, rostral toward the left, dorsal toward the top. Scale bar: (B-M) 100 μm.

Previous studies suggested that Lbx1 regulates the migration of limb, hypoglossal, and head muscle precursors in mouse and Xenopus [12, 13, 15]. Our observation that zebrafish lbx2 appears in fin bud and hyoid muscles suggests that Lbx2 may be involved in regulation of hypaxial and head muscle development. We find that inactivation of Lbx2 blocks formation of the fin bud and blocks myod expression in hyoid muscles (Fig. 2.B–E, arrows and arrowheads respectively). Embryos injected with lbx2-MO express fgf8 normally in the ectoderm (Fig. 2H–K, arrows)[26], indicating that other aspects of fin bud development are unaffected by MO injection. In addition to lbx2, zebrafish lbx1b is also expressed in the fin bud (Fig. 1P–R). Injection of lbx1b-MO results in decreased myod expression in the fin bud, and again, fgf8 expression is normal (Fig. 2F, G, L, M). Thus, zebrafish Lbx2 and Lbx1b are both required for proper development of hypaxial muscles that are derived from lbx2 and/or lbx1b expressing myoblasts, similar to Xenopus and mouse Lbx1 [12, 13, 15].

Functional roles of Lbx2 in the induction of myogenesis and the migration of slow muscle precursors

Our observation of transient expression of lbx2 in paraxial mesoderm and adaxial cells at the end of bud stage suggests that Lbx2 might be involved in specification of muscle cells at this stage. [27]. However, we find no obvious changes in expression of myogenic regulatory factors such as myod expression in adaxial cells or myf5 expression in paraxial mesoderm in lbx2 knock down embryos (Fig. 3A–D), although a few myod expressing cells fail to incorporate properly into the adaxial cell layer (Fig. 3H, arrow) and the distinctive cuboidal shape of the adaxial cell pseudo-epithelium is somewhat disrupted in (Fig. 3E, F, arrow). Transplantation analysis showed that, although morphogenesis of cells lacking Lbx2 activity is affected, they still express myod when located adjacent to the notochord (see Additional file 1). These results indicate that Lbx2 is not involved in the induction of myogenesis.
Figure 3

Lbx2 is not required for the induction of myogenesis. A-D: Suppression of Lbx2 activity does not affect myod or myf5 expression. The adaxial cells (precursors of slow muscles and muscle pioneers) express myod (A, 100%, n = 20). Embryos injected with lbx2-MO express myod at normal levels (B, 100%, n = 20). The paraxial mesoderm expresses myf5 in control embryos (C, 100%, n = 17) and lbx2-MO injected embryos (D, 100%, n = 12). E-F: Adaxial cell morphology at 3-somite stage in control (E) or lbx2-MO injected (F) embryos. Nomarski images of dorsal views. The brackets indicate the width of the adaxial cell row. The normally cuboidal adaxial cells are aberrantly shaped in the lbx2-MO injected embryo. Arrows indicate individual adaxial cells. G-H: Several myod expressing cells fail to incorporate properly into the adaxial cell monolayer after Lbx2 knockdown. (A-H) Whole-mount embryos, dorsal views, rostral toward the top. Scale bar: (A-D) 200 μm (E, F) 25 μm, (G, H) 50 μm.

During segmentation stages, the slow muscle precursors, a subset of adaxial cells, migrate radially away from the notochord to form the superficial layer of slow muscle [18]. Because adaxial cells transiently express lbx2 (Fig. 1J, K), we examined whether Lbx2 is involved in their migration. Cross sections of lbx2-MO injected embryos indicate that muscle precursors migrate properly through the somite to form the superficial layer (Fig. 4D, H, L). Transplantation analysis also showed that cells lacking Lbx2 function migrate properly to the superficial layer in uninjected embryos (not shown). Thus, in the absence of Lbx2 activity, mesodermal cells form muscle precursors, as indicated by myod and myf5 expression, and slow muscle precursors migrate normally to the lateral surface of the somite.
Figure 4

Knockdown of Lbx2 activity results in malformation of slow muscle fibers. A-D: Control embryos. E-H: lbx2-MO injected embryos. I-L: lbx2 mRNA injected embryos. (A-B, E-F, I-J) Embryos labeled with the slow muscle marker, F59 (red). (B, F, J) Higher magnification. Rostral-caudal extension of slow fibers is severely affected by lbx2 knockdown (B, F, arrows). M: Statistical analyses of mean filament rostral-caudal length (arrows) and dorsoventral thickness (B, F, arrowheads). Analysis by ANOVA demonstrates significant differences in length (P < 0.01) and thickness (P < 0.05). (C, G, K, N) Embryos labeled with Prox1 (green, nuclear slow muscle and muscle pioneer marker), and 4D9 (red, Eng, muscle pioneer marker,). Green indicates slow muscle cells and yellow shows muscle pioneers (MP). (ctrl: n = 10, lbx2-MO: n = 6, lbx2-mRNA: n = 4). No apparent differences can be detected between controls and embryos injected with lbx2-MO. N: Analysis by ANOVA demonstrates no significant differences. The data represent the average +/- s.e.m. (D, H, L) Transverse sections of a 24 hpf embryo labeled with the slow muscle marker, F59 (green) and Hoechst to mark nuclei. Slow muscle cells migrate properly to the superficial layer. (A-C, E-G, I-K) Lateral views, rostral toward the left, dorsal toward the top; (D, H, L) dorsal toward the top. Scale bar: (A, C-E, G-I, K-L) 50 μm; (B, F, J) 25 μm.

Absence of Lbx2 activity results in malformed slow muscle cells

To explore the functions of Lbx2 further, we examined the number and structure of differentiated slow muscle fibers. Double labeling with Prox1 (labels all slow muscle cell nuclei, [28]) and 4D9 (engrailed, labels muscle pioneer nuclei and medial fast fiber cells, [29]) demonstrated that the number of slow muscle (green) and muscle pioneer cells (yellow) is unaffected by injection of lbx2 splice donor MO or lbx mRNA (Fig. 4C, G, K, N), indicating normal proliferation of slow muscle precursors and differentiation of slow muscle precursors into slow muscle and muscle pioneers. Thus, the specification and numbers of slow muscle cells and muscle pioneers are regulated independently of Lbx2. On the other hand, however, we find that lbx2 splice donor MO injected embryos fail to form normally shaped slow muscle fibers (Fig. 4A, B, E, F, I, J, M). Injection of lbx2 translation blocking MO produced the same phenotypes as lbx2 splice donor MO (see Additional file 2). Further analysis showed that although slow muscle cells normally contain extended and thickened myofibrils by 24 hpf (Fig. 4B, M and see Additional file 3; length: 60 +/- 0.8 μm, arrow; thickness: 3.52 +/- 0.4 μm, arrowhead; average +/- s.e.m.), lbx2 splice donor MO injected embryos exhibit shorter and thinner myofibrils (Fig. 4F, M; length: 32 +/- 4.5 μm, thickness: 1.5 +/- 0.1 μm, Fig. 4M). These malformed slow muscle fibers are still present at 48 hpf (Fig. 4M and see Additional file 4; control: 58 +/- 2.3 μm, 5.1 +/- 0.3 μm; lbx2 splice donor MO injected embryos: 46 +/- 1.5 μm, 3.27 +/- 0.4 μm). It is unlikely that these defects are due to a general developmental delay because other aspects of development, including muscle precursor cell migration (Fig. 4H) and rostral caudal extension of the somites (Fig. 4E) occur normally. Thus, Lbx2 contributes to formation of myofibrils in slow muscle cells.

Fast muscle fibers form abnormal in the absence of Lbx2

Because fast muscle cells transiently express lbx2 around 24 hpf (Fig. 1E, L), we also examined the structure of fast muscle cells. The EB165 antibody specifically recognizes fast myosin heavy chain protein (MyHC)[30]. We find that, although fast muscle cells in control embryos contain extended myofibrils by 24 hpf (length: 52 +/- 1.1 μm.), lbx2 splice donor MO injected embryos form shorter myofibrils (length: 29 +/- 3.1 μm; Fig. 5A, B, D, E, G, H, J), and these shortened myofibrils are still present at 48 hpf (control: 84 +/- 5.4 μm, lbx2-MO injected embryos: 61 +/- 1.9 μm). However, we observe no apparent difference in thickness between control and lbx2 splice donor MO injected embryos (24 hpf, control: 1.9 +/- 0.2 μm, lbx2-MO injected embryo: 1.8 +/- 0.2 μm; 48 hpf, control: 2.4 +/- 0.1 μm, lbx2-MO injected embryo: 2.6 +/- 0.2 μm). Thus, formation of myofibrils in fast muscle cells also depends upon Lbx2 function.
Figure 5

Fast muscle fibers are malformed in the absence of Lbx2. A-C: Control embryos. D-F: lbx2-MO injected embryos. G-I: lbx2 mRNA injected embryos. (A, B, D, E, G, H, J) Embryos labeled with the fast muscle marker, EB165 (red). (B, E, H) Higher magnification views. Rostral caudal extension of fast fibers is severely affected (B, E, arrow). J: Statistical analyses of mean filament rostral-caudal length (B, E, arrows) or dorsal ventral thickness (B, E, arrowhead). Analysis by ANOVA demonstrates significant differences in length (P < 0.01). (C, F, I) Embryos were mosaically labeled with membrane localized GFP (mGFP) by injection of DNA and labeled for nuclei (red, propidium iodide). mGFP expressing cells in the fast muscle domain at the level of somite 10 were randomly selected and the number of their nuclei counted. K: Fast muscle cells are multinucleate by 48 hpf in both control and lbx2 mRNA injected embryos, whereas unfused fast muscle cells are observed in lbx2-MO injected embryo (F, arrow). Analysis demonstrates significant differences among the three groups at 48 hpf (Kruskal-Wallis test, p = 0.0121). No apparent differences can be detected between controls and embryos injected with lbx2-MO at 24 hpf (Kruskal-Wallis test, p = 0.1509). (A-I) Lateral views, rostral toward the left, dorsal toward the top. Scale bars: (A, B, D, E, G, H) 50 μm, (C, F, I) 20 μm.

In zebrafish, fast muscle cell precursors are fusing by 24 hpf, and the majority of fast muscle cells are multinucleate [2]. In Drosophila that lack Lbx function, myoblasts fail to fuse [8]. Therefore, we examined whether fusion of fast muscle cells is affected by knockdown of Lbx2 activity in zebrafish. Fast muscle cells in somite 10 are multinucleate by 24 hpf and 48 hpf in both control and lbx2-mRNA injected embryos, whereas many fast muscle cells remain unfused in lbx2 splice donor MO injected embryos (Fig. 5C, F, I, K; the number of nuclei at 48 hpf is significantly different, Kruskal-Wallis test, p = 0.012, see Additional file 5). In addition, unfused cells sometimes exhibit a curved shape. Thus, both the formation of myofibrils and the fusion of fast muscle precursors depend upon Lbx2 activity.

Interference with Lbx2 activity downregulates myofilament gene expression

Our finding that knockdown of Lbx2 activity results in malformed slow and fast myofibrils suggested that expression of genes encoding components of the sarcomere may depend upon Lbx2 function. We therefore examined the expression of genes encoding components of thin, thick, and elastic filaments. skeletal muscle alpha-actin (acta1), skeletal muscle troponin T (tnnt1), fast skeletal muscle tropomyosin (tpma), skeletal fast troponin T3b (tnntt3b), and fast skeletal muscle troponin C (tnnc) encode thin filament proteins [24, 31]. Both slow and fast muscle cells express acta1, slow muscle cells express tnnt1, and fast muscle cells express tnnt3b [31]. We find that expression of the thin filament genes, acta1, tnnt1, tpma, and tnnt3b is reduced in Lbx2 deficient skeletal muscles, although tnnc expression appears normal (Fig. 6A–E). skeletal muscle myosin heavy chain (myhz1), slow type myosin binding protein C (mybpc1), slow myosin heavy chain 1 (smyhc1), fast muscle specific myosin heavy polypeptide 2 (myhz2), and fast skeletal muscle myosin light chain 2 (mylz2)encode thick filament proteins [7, 31, 32]. Slow muscle cells, including muscle pioneers, specifically express mybpc1 [7], and fast muscle cells express myhz2 [31]. Expression of the thick filament genes, myhz1, mybpc1, and myhz2 is reduced in embryos lacking Lbx2, whereas smyhc and mylz2 expression is normal (Fig. 6F–J). titin encodes a component of elastic filaments [33]. In contrast to thin and thick filaments, expression of titin is not affected by lbx2-MO. Thus, deficiency of Lbx2 activity results in decreased expression of specific thin and thick filament genes.
Figure 6

Expression of thin and thick myofilament genes is downregulated by interference with Lbx2 activity. A-J: Analysis of myofilament gene expression. Expression of acta1 (A), tnnt1 (B), tpma (C), tnnt3b (D), tnnc (E), myhz1 (F), mybpc1 (G), smyhc (H), myhz2 (I), mylz2 (J) in control, lbx2-MO injected, lbx2 mRNA injected, and Engrailed suppresser domain fused lbx2 (EnR-lbx2) mRNA injected embryos. Expression of acta1 (A, ctrl: 28/28, lbx2-MO: 0/33, lbx2-mRNA: 9/10, EnR-lbx2 mRNA: 8/8, numerator indicates number of embryos with normal expression, and denominator indicates number of examined embryos), tnnt1 (B, ctrl: 19/19, lbx2-MO: 7/22, lbx2-mRNA: 12/12, EnR-lbx2 mRNA: 17/17), tpma (C, ctrl: 16/16, lbx2-MO: 2/20, lbx2-mRNA: 13/14, EnR-lbx2 mRNA: 10/11) and tnnt3b (D, ctrl: 13/13, lbx2-MO: 0/7, lbx2-mRNA: 7/7, EnR-lbx2 mRNA: 4/4) is reduced by lbx2-MO, but not tnnc (E, ctrl: 17/17, lbx2-MO: 18/22, lbx2-mRNA: 10/10, EnR-lbx2 mRNA: 16/16). Expression of myhz1 (F, ctrl: 25/25, lbx2-MO: 3/16, lbx2-mRNA: 7/8, EnR-lbx2 mRNA: 8/8), mybpc1 (G, ctrl: 15/15, lbx2-MO: 10/20, lbx2-mRNA: 13/14, EnR-lbx2 mRNA: 5/8) and myhz2 (I, ctrl: 17/17, lbx2-MO: 0/5, lbx2-mRNA: 9/9, EnR-lbx2 mRNA: 8/8) is reduced by lbx2-MO, but smyhc (H, ctrl: 36/36, lbx2-MO: 24/29, lbx2-mRNA: 8/8, EnR-lbx2 mRNA: 12/12) and mylz2 are unaltered (J, ctrl: 15/15, lbx2-MO: 14/15, lbx2-mRNA: 10/10, EnR-lbx2 mRNA: 7/7). No obvious difference can be detected between embryos injected with lbx2 mRNA and embryos injected with EnR-lbx2 mRNA. (A-J) Whole mounts, lateral views, rostral toward the left, dorsal toward the top. Scale bar: 100 μm.

The engrailed domain of Lbx2 is required for regulation of myofilament gene expression

In Xenopus, overexpression of Lbx1 causes a decrease of myoD expression and an increase of myf5 expression in hypaxial myoblasts, and the engrailed repressor domain of Lbx1 is required for this function [15]. To learn whether the engrailed domain of zebrafish Lbx2 is required for myofilament formation, we tested whether Lbx2 protein that lacks the engrailed domain, rescues lbx2-MO injected embryos. lbx2-MO results in decreased atca1 and mybp1 expression (Fig. 7B, F) and lbx2 mRNA rescues atca1 and mybp1 expression in lbx2-MO injected embryos (Fig. 7C, G, arrow). However, mRNA encoding Lbx2 that lacks the engrailed domain fails to rescue atca1 and mybp1 expression in lbx2-MO injected embryos (Fig. 7D, H). In contrast to atca1 and mybp1, mef2c and myogenin expression is upregulated by lbx2-MO injection (Fig. 7I, J, M, N) and suppressed by overexpression of lbx2 mRNA (see Additional file 6). lbx2 mRNA rescues mef2c and myogenin in lbx2-MO injected embryos (Fig. 7K, O, arrow). However, mRNA encoding Lbx2 that lacks the engrailed domain fails to rescue mef2c and myogenin in lbx2-MO injected embryos. Thus, the engrailed domain of Lbx2 is required for Lbx2 function during the formation of myofilaments.
Figure 7

The Engrailed domain of Lbx2 is required for myofilament gene expression. A-H: Expression of acta1 (A-D) and mybpc1 (E-H). Embryos express acta1at 24 hpf (A: 93% with normal expression, n = 15). Injection of lbx2-MO results in decreased acta1 expression (B: 20% with normal expression, n = 20), whereas acta1 expression partially recovers in embryos injected with lbx2-MO + lbx2 mRNA (C: 42% with normal expression, n = 50). In contrast, embryos injected with lbx2-MO + lbx Δ En mRNA fail to express acta1 (D: 14% with normal expression, n = 37). mybpc1 (E: 94%, n = 18; F: 43%, n = 23; G: 81%, n = 31; H: 42%, n = 38). I-P: Expression of mef2ca (I-L) and myogenin (M-P). Embryos express mef2ca at 24 hpf (I: n = 11). Injection of lbx2-MO results in increased mef2ca expression (J: 72% with increased expression, n = 11), whereas mef2ca expression partially recovers in embryos injected with lbx2-MO + lbx2 mRNA (K: 21% with increased expression, n = 19). In contrast, embryos injected with lbx2-MO + lbx Δ En mRNA fail to express mef2ca (L: 61% with increased expression, n = 21). myogenin (M: n = 7, N: 78% with increased expression, n = 9, O: 23%, n = 13, P: 67%, n = 14). (A-P) Whole mounts, lateral views, rostral toward the left, dorsal toward the top. Scale bar: 100 μm.

Discussion

Previous studies suggested that Lbx1 regulates the migration of limb and hypoglossal myoblasts in mouse [12, 13]. Recently, an additional function of Lbx1 was reported, whereby Lbx1 represses myod expression and promotes myoblast proliferation before the onset of terminal differentiation [15]. In contrast, functions of Lbx2 are poorly understood, because Lbx2 deficiency does not impair mouse development [14]. Here, we demonstrate in zebrafish that lbx2 is expressed in mesodermal cells, migratory slow muscle precursors, fin bud, and hyoid [23], whereas lbx1b is expressed only in fin bud during myogenesis. Loss of Lbx2 function does not apparently affect induction of myogenesis, slow muscle migration, or proliferation, but rather reduces cell fusion, as in Drosophila, and, furthermore, Lbx2 knockdown blocks differentiation of myofibrils due to reduced expression of specific thin and thick filament genes.

Slow and fast muscle precursor cells transiently express Lbx2

lbx2 expression appears by the end of bud stage in paraxial mesoderm and in adaxial cells, the precursors of slow muscle including muscle pioneers. A subset of adaxial cells migrates through the somite during segmentation stages [18], but lbx2 is not expressed in nor required for migration of these cells. Interestingly, a subset of fast muscle cells begins to express lbx2 in both epaxial and hypaxial domains by the end of segmentation stages. A previous study in zebrafish concluded that lbx2 expression is restricted to ventral regions of the somites [23]. This discrepancy could be due to the rather weak expression of lbx2 in the epaxial domain (Fig. 1E), which may have been missed in the earlier study. By 48 hpf, expression of lbx2 disappears from somites in the trunk. Similarly in Xenopus, the ventrolateral region of rostral trunk somites, which contains precursors of hypaxial body wall, expresses lbx1, and this expression later becomes weak and eventually lost [15]. Satellite cells in mouse that express Lbx1 also extinguish expression during myogenic differentiation [34]. Thus, Lbx gene expression may be transient during myogenesis in all species.

During muscle development in chick, the myotome forms in three waves [35, 36]. The first wave of early post-mitotic progenitors appears along the entire dorsomedial aspect of the epithelial somite. Then in the second wave, cells migrate from all four lips of the dermomyotome, although myofibers are generated from only the rostral and caudal edges. During the third wave, there is a balance between differentiation and proliferation. Similarly, zebrafish muscle development can be divided into several steps. First, epithelial adaxial cells, precursors of slow and muscle pioneers, are specified to be slow or muscle pioneers by Hh signaling from the notochord [19]. Then, slow muscle precursors migrate to the lateral surface of the somite [18]. Second, a wave of fast muscle morphogenesis is induced by the migration of the slow muscle precursors [37]. Finally, new slow-muscle fibers are added in growth zones near the dorsal and ventral extremes of the myotome, and this muscle growth continues into larval life [38]. We demonstrated that lbx2 expression first appears in adaxial cells. Adaxial cells lose lbx2 expression before they incorporate into the somite. Then, a subset of fast muscle precursors begins to express lbx2. Further analysis is required to determine whether Lbx2 is transiently expressed in all or only a subset of muscle precursors and whether such transient expression is linked to particular waves of muscle development.

Migration of slow muscle precursors in Lbx2 knockdown embryos

In mouse, disruption of Lbx1 causes a severe loss of limb muscle, because muscle progenitors fail to migrate [13]. We showed that disruption of Lbx2 activity causes decreased myod expression in fin bud and hyoid muscles (Fig. 3), suggesting that Lbx2 could be involved in migration of these precursors in zebrafish. Additionally, we previously reported that a subset of adaxial cells migrate laterally to form slow muscle [18]. The expression of lbx2 in adaxial cells raised the possibility that Lbx2 may regulate migration of slow muscle precursors, too. Although transplantation analysis showed that the early movement of cells lacking Lbx2 is disrupted during gastrulation (see Additional file 1), slow muscle precursors eventually migrate radially (Fig. 4). Therefore, although it is likely that Lbx2 contributes to the migration of fin and head muscle precursors, Lbx2 is not required for migration of slow muscle precursors.

A molecular link between Lbx2, a transcriptional repressor, and filament gene expression

The Lbx1 protein contains the engrailed repressor domain [39] and Lbx1 protein that lacks the engrailed domain fails to suppress myod expression in Xenopus [15]. Moreover, Lbx1 interacts with Corl1, a transcriptional corepressor [40]. These observations strongly suggest that Lbx1 functions as a transcriptional repressor. We find that Lbx2 that lacks the engrailed domain fails to induce atca1 and mybpc1 in lbx2-MO injected embryos, and also fails to suppress mef2c and myogenin in lbx2-MO injected embryos (Fig. 7). This result indicates that the engrailed repressor domain is required for function of Lbx2 in the formation of myofibrils. However, our observation that lbx2-MO leads to a decrease in filament gene expression suggests that filament genes cannot be direct targets of Lbx2. Instead, this result indicates that Lbx2 acts through an intermediate factor (or factors) to regulate expression of filament genes.

Myogenin and Mef2 are known to function in filament formation [7, 41, 42], and we showed that expression of both myogenin and mef2c is reduced by overexpression of Lbx2. Thus, Myogenin and Mef2c may act downstream of Lbx2 to regulate myofilament gene expression during formation of myofibrils. However, Myogenin and Mef2c are thought to function as transcriptional activators. Our observations that myogenin and mef2c expression is upregulated in lbx2-MO injected embryos, whereas filament gene expression is decreased by lbx2-MO, suggest that there must be other factors downstream of Lbx2, most likely additional transcriptional repressors. Thus, further studies are required to identify the proximal downstream mediators of Lbx2 function.

Conclusion

Several steps are required for muscle progenitors to form functional skeletal muscle: (1) formation of a population of multipotent mesodermal cells, (2) specification and commitment to a muscle fate, and (3) differentiation accompanied by cell fusion and assembly of contractile myofibrils. The precise timing of these steps requires a fine balance between transcriptional activation and repression [43, 44]. Although many transcriptional activators and repressors involved in commitment, specification, and differentiation of muscle precursors have been identified [45], the transcriptional network that regulates terminal differentiation is still incompletely understood. Our study has identified Lbx2 as a regulator of myofibril formation through its action on expression of myofilament genes, including myosin and actin. A recent study showed that undifferentiated satellite cells express Lbx1, and Lbx1 expression diminishes as cells differentiate [34]. In addition, it is known that Lbx1 upregulates Pax7 expression and downregulates Myod in satellite cells [34], and we also find suppression of pax7 expression in lbx2-MO injected embryos (see Additional file 6). Thus, it is possible that Lbx1 maintains the immature state of satellite cells by regulating Pax7. Based on these observations, we suggest that the general function of Lbx2 may be to aid differentiation of myoblasts by promoting fusion and myofilament gene expression.

Methods

Animals

Embryos were obtained from the University of Oregon zebrafish facility, produced using standard procedures [46] and staged according to standard criteria [47]. The wild-type line used was AB.

Plasmid construction

Gene-specific primers to amplify full-length zebrafish lbx2 (AJ29516) and part of lbx1b cDNA were designed based on the available Ensemble zebrafish genome assembly (Zv4).

Full-length zebrafish lbx2 was obtained from 24 post-fertilization (hpf) embryos by RT-PCR with primers lbx2 F (5'-ATG ACC TCC AGC TCT AAA GA-3') and lbx2 R (5'-TTA ATC GTC GAC CTC GAT TT-3').

The PCR product was cloned into the EcoRI site of pCS2 (pCS2-lbx2) and pCS2+EnR (pCS-EnR-lbx2) [48]. To make removed engrailed domain, amino acids 47–257 of zebrafish lbx2 was amplified by PCR with the primers lbx2 Δ En (5'-GGT ACC ATG ATC TTA AAC AAG CCC TCC GTT) and lbx2 R, and cloned into the Asp718 and XbaI site of pCS (pCS-lbx2 Δ En domain). A part of zebrafish lbx1b was obtained from 24 post-fertilization (hpf) embryos by RT-PCR with primers lbx1bF (5'-CTCCACCTGCTAACTCAAAC3') and lbx1b R(5'-TCAGTCATCTACATCAATTTCCTCG-3'). Zebrafish genome informatics analysis (based on the Zv6 assembly) reveals that lbx1b lies chromosome 13 at location 20, 761, 204–20, 762, 898. basepairs (bp) and lbx2 lies on chromosome 14 at location 1, 185, 681–1, 188, 749 bp [22].

In vitromRNA synthesis

Capped mRNAs were transcribed from linearized DNA templates with SP6 RNA polymerase in vitro transcription kits (mMESSAGE mMACHINE SP6, Ambion, Inc., Austin, TX USA) according to the manufacturer's instructions. pCS2-lbx2 plasmid was linearized with XbaI. pCS2-EnR-lbx2 plasmid was linearized with Asp718. pCS-lbx2 Δ En domain was linearized with XbaI.

In situmRNA hybridization

The in situ labeling was performed as previously described [49] using the markers: myod, myf5, myogenin [27], no tail (ntl) [50], skeletal muscle alpha-actin (acta1), skeletal muscle troponin T (tnnt: currently named skeletal slow troponin T1, tnnt1), fast skeletal muscle tropomyosin (tpma), skeletal fast troponin T3b (tnntt3b), fast skeletal muscle troponin C (tnnc) [31, 24] (ZFIN), skeletal muscle myosin heavy chain (currently named myosin, skeletal muscle heavy polypeptide 1, myhz1), slow myosin heavy chain 1 (smyhc1), slow type myosin binding protein C (mybpc1), fast skeletal muscle myosin light chain 2 (currently named skeletal muscle myosin light polypeptide 2, mylz2), fast muscle specific myosin heavy polypeptide 2 (myhz2) [7, 31, 32]. Probes were synthesized using SP6 RNA polymerase or T7 RNA polymerase. Embryos processed for whole-mount in situ hybridization were photographed using a Leica MZFGIII microscope and Axiocam digital camera.

Microinjection

mRNA was dissolved in double distilled H2O to final concentrations of 30 ng/μl to 50 ng/μl. Phenol red was added to the solution. Approximately 1 nl of RNA or DNA was injected at the one-cell stage using published procedures [51]. lbx2-MO was directed to the translation start site, splice donor site (Gene Tools, LLC), lbx2-ATG MO: TCATGTCTTTAGAGCTGGAGGTCAT, lbx2-splice donor MO: TTATGAACTTTTTACCTTCTGCTGC, lbx1b-splice donor MO: ACACCGGGCCTTGTGTTTACCTTCT. Stock solutions were resuspended at 2.5 μg/μl.

To obtain mosaic expression, we performed the plasmid injected (100 ng/μl).

Antibody labeling and quantification of muscle fiber numbers

Labeling with F59, EB165, 4D9 and Prox1 was as previously described [3, 5, 17]. The primary antibodies were mAb F59 (anti-MyHC, slow muscle) at 1:20, mAb EB165 (anti-MyHC, fast muscle) at 1:5000, mAb 4D9 (anti-Engrailed) at a dilution of 1:20, rabbit anti-Prox1 (AngioBio Co.) at a dilution of 1:500. Secondary antibodies were AlexaFluor-594 goat anti mouse IgG at 1:1000 and AlexaFluore-488 goat anti-rabbit IgG at 1:1000 (Molecular Probes). The images ware collected using a LSM 5 PASCAL confocal microscope configured around an AXIO Imager M1 upright microscope. The numbers of slow muscle and muscle pioneers were calculated from the total number of cells labeled by the Prox1 antibody (slow muscle) and the total number of cells labeled by both the Prox1 and 4D9 antibodies (muscle pioneer cell) per embryo counted in four somites over the extended yolk at 24 hpf. Data represent the average number of slow muscle cells or muscle pioneer cells per somite. To quantify the phenotypes of muscle fibers, we examined somites over the extended yolk in 3–5 embryos. Fibers that were positioned in the middle of the dorsal half of the somite were measured. The lengths of muscle fibers were measured from the anterior somite border to the end of each fiber (Fig. 4B arrow). Because fiber thickness depends on how many sarcomeres line up in parallel [52], we measured the diameter of each fiber from its dorsal to its ventral edge (Fig. 4B arrowhead).

Declarations

Acknowledgements

We thank Simon M. Hughes, Zhiyan Gong and Yi-Lin Yan for filament gene probes. Supported by NIH HD22486 and AR45575 (Grant sponsor).

Authors’ Affiliations

(1)
Institute of Neuroscience, University of Oregon
(2)
Developmental Genomics Research Group, Nara Institute of Science and Technology, 8916-5

References

  1. Ochi H, Westerfield M: Signaling networks that regulate muscle development: lessons from zebrafish. Dev Growth Differ. 2007, 49: 1-11.View ArticlePubMedGoogle Scholar
  2. Moore CA, Parkin CA, Bidet Y, Ingham PW: A role for the Myoblast city homologues Dock1 and Dock5 and the adaptor proteins Crk and Crk-like in zebrafish myoblast fusion. Development. 2007, 134: 3145-53.View ArticlePubMedGoogle Scholar
  3. Ochi H, Pearson BJ, Chuang PT, Hammerschmidt M, Westerfield M: Hhip regulates zebrafish muscle development by both sequestering Hedgehog and modulating localization of Smoothened. Dev Biol. 2006, 297: 127-40.View ArticlePubMedGoogle Scholar
  4. Ochi H, Hans S, Westerfield M: Smarcd3 regulates the timing of zebrafish myogenesis onset. J Biol Chem. 2008, 283: 3529-36.View ArticlePubMedGoogle Scholar
  5. Srinivas BP, Woo J, Leong WY, Roy S: A conserved molecular pathway mediates myoblast fusion in insects and vertebrates. Nat Genet. 2007, 39: 781-6.View ArticlePubMedGoogle Scholar
  6. Sartorelli V, Webster KA, Kedes L: Muscle-specific expression of the cardiac alpha-actin gene requires MyoD1, CArG-box binding factor, and Sp1. Genes Dev. 1990, 4: 1811-22.View ArticlePubMedGoogle Scholar
  7. Hinits Y, Hughes SM: Mef2s are required for thick filament formation in nascent muscle fibres. Development. 2007, 134: 2511-9.PubMed CentralView ArticlePubMedGoogle Scholar
  8. Jagla T, Bellard F, Lutz Y, Dretzen G, Bellard M, Jagla K: ladybird determines cell fate decisions during diversification of Drosophila somatic muscles. Development. 1998, 125: 3699-708.PubMedGoogle Scholar
  9. Garcia-Fernandez J: The genesis and evolution of homeobox gene clusters. Nat Rev Genet. 2005, 6: 881-92.View ArticlePubMedGoogle Scholar
  10. Maqbool T, Soler C, Jagla T, Daczewska M, Lodha N, Palliyil S, VijayRaghavan K, Jagla K: Shaping leg muscles in Drosophila: role of ladybird, a conserved regulator of appendicular myogenesis. PLoS ONE. 2006, 1: e122-PubMed CentralView ArticlePubMedGoogle Scholar
  11. Chen F, Liu KC, Epstein JA: Lbx2, a novel murine homeobox gene related to the Drosophila ladybird genes is expressed in the developing urogenital system, eye and brain. Mech Dev. 1999, 84: 181-4.View ArticlePubMedGoogle Scholar
  12. Birchmeier C, Brohmann H: Genes that control the development of migrating muscle precursor cells. Curr Opin Cell Biol. 2000, 12: 725-30.View ArticlePubMedGoogle Scholar
  13. Gross MK, Moran-Rivard L, Velasquez T, Nakatsu MN, Jagla K, Goulding M: Lbx1 is required for muscle precursor migration along a lateral pathway into the limb. Development. 2000, 127: 413-24.PubMedGoogle Scholar
  14. Wei K, Chen J, Akrami K, Sekhon R, Chen F: Generation of mice deficient for Lbx2, a gene expressed in the urogenital system, nervous system, and Pax3 dependent tissues. Genesis. 2007, 45: 361-8.View ArticlePubMedGoogle Scholar
  15. Martin BL, Harland RM: A novel role for lbx1 in Xenopus hypaxial myogenesis. Development. 2006, 133 (2): 195-208. Epub 2005 Dec 8View ArticlePubMedGoogle Scholar
  16. Ingham PW, McMahon AP: Hedgehog signaling in animal development: paradigms and principles. Genes Dev. 2001, 15: 3059-87.View ArticlePubMedGoogle Scholar
  17. Du SJ, Devoto SH, Westerfield M, Moon RT: Positive and negative regulation of muscle cell identity by members of the hedgehog and TGF-beta gene families. J Cell Biol. 1997, 139: 145-56.PubMed CentralView ArticlePubMedGoogle Scholar
  18. Devoto SH, Melancon E, Eisen JS, Westerfield M: Identification of separate slow and fast muscle precursor cells in vivo, prior to somite formation. Development. 1996, 122: 3371-80.PubMedGoogle Scholar
  19. Hirsinger E, Stellabotte F, Devoto SH, Westerfield M: Hedgehog signaling is required for commitment but not initial induction of slow muscle precursors. Dev Biol. 2004, 275: 143-57.View ArticlePubMedGoogle Scholar
  20. Stellabotte F, Dobbs-McAuliffe B, Fernandez DA, Feng X, Devoto SH: Dynamic somite cell rearrangements lead to distinct waves of myotome growth. Development. 2007, 134: 1253-7.View ArticlePubMedGoogle Scholar
  21. Hollway GE, Bryson-Richardson RJ, Berger S, Cole NJ, Hall TE, Currie PD: Whole-somite rotation generates muscle progenitor cell compartments in the developing zebrafish embryo. Dev Cell. 2007, 12: 207-19.View ArticlePubMedGoogle Scholar
  22. Wotton KR, Weierud FK, Dietrich S, Lewis KE: Comparative genomics of Lbx loci reveals conservation of identical Lbx ohnologs in bony vertebrates. BMC Evol Biol. 2008, 8: 171-PubMed CentralView ArticlePubMedGoogle Scholar
  23. Neyt C, Jagla K, Thisse C, Thisse B, Haines L, Currie PD: Evolutionary origins of vertebrate appendicular muscle. Nature. 2000, 408: 82-6.View ArticlePubMedGoogle Scholar
  24. Thisse B, Heyer V, Lux A, Alunni V, Degrave A, Seiliez I, Kirchner J, Parkhill JP, Thisse C: Spatial and temporal expression of the zebrafish genome by large-scale in situ hybridization screening. Methods Cell Biol. 2004, 77: 505-19.View ArticlePubMedGoogle Scholar
  25. Kimmel CB, Warga RM, Schilling TF: Origin and organization of the zebrafish fate map. Development. 1990, 108: 581-94.PubMedGoogle Scholar
  26. Reifers F, Bohli H, Walsh EC, Crossley PH, Stainier DY, Brand M: Fgf8 is mutated in zebrafish acerebellar (ace) mutants and is required for maintenance of midbrain-hindbrain boundary development and somitogenesis. Development. 1998, 125: 2381-95.PubMedGoogle Scholar
  27. Weinberg ES, Allende ML, Kelly CS, Abdelhamid A, Murakami T, Andermann P, Doerre OG, Grunwald DJ, Riggleman B: Developmental regulation of zebrafish MyoD in wild-type, no tail and spadetail embryos. Development. 1996, 122: 271-80.PubMedGoogle Scholar
  28. Wolff C, Roy S, Ingham PW: Multiple muscle cell identities induced by distinct levels and timing of hedgehog activity in the zebrafish embryo. Curr Biol. 2003, 13: 1169-81.View ArticlePubMedGoogle Scholar
  29. Hatta K, Bremiller R, Westerfield M, Kimmel CB: Diversity of expression of engrailed-like antigens in zebrafish. Development. 1991, 112: 821-32.PubMedGoogle Scholar
  30. Blagden CS, Currie PD, Ingham PW, Hughes SM: Notochord induction of zebrafish slow muscle mediated by Sonic hedgehog. Genes Dev. 1997, 11: 2163-75.PubMed CentralView ArticlePubMedGoogle Scholar
  31. Xu Y, He J, Wang X, Lim TM, Gong Z: Asynchronous activation of 10 muscle-specific protein (MSP) genes during zebrafish somitogenesis. Dev Dyn. 2000, 219: 201-15.View ArticlePubMedGoogle Scholar
  32. Bryson-Richardson RJ, Daggett DF, Cortes F, Neyt C, Keenan DG, Currie PD: Myosin heavy chain expression in zebrafish and slow muscle composition. Dev Dyn. 2005, 233: 1018-22.View ArticlePubMedGoogle Scholar
  33. Yan YL, Miller CT, Nissen RM, Singer A, Liu D, Kirn A, Draper B, Willoughby J, Morcos PA, Amsterdam A, et al: A zebrafish sox9 gene required for cartilage morphogenesis. Development. 2002, 129: 5065-79.PubMedGoogle Scholar
  34. Watanabe S, Kondo S, Hayasaka M, Hanaoka K: Functional analysis of homeodomain-containing transcription factor Lbx1 in satellite cells of mouse skeletal muscle. J Cell Sci. 2007, 120: 4178-87.View ArticlePubMedGoogle Scholar
  35. Kahane N, Cinnamon Y, Bachelet I, Kalcheim C: The third wave of myotome colonization by mitotically competent progenitors: regulating the balance between differentiation and proliferation during muscle development. Development. 2001, 128: 2187-98.PubMedGoogle Scholar
  36. Kahane N, Cinnamon Y, Kalcheim C: The roles of cell migration and myofiber intercalation in patterning formation of the postmitotic myotome. Development. 2002, 129: 2675-87.PubMedGoogle Scholar
  37. Henry CA, Amacher SL: Zebrafish slow muscle cell migration induces a wave of fast muscle morphogenesis. Dev Cell. 2004, 7: 917-23.View ArticlePubMedGoogle Scholar
  38. Barresi MJ, D'Angelo JA, Hernandez LP, Devoto SH: Distinct mechanisms regulate slow-muscle development. Curr Biol. 2001, 11: 1432-8.View ArticlePubMedGoogle Scholar
  39. Jagla K, Bellard M, Frasch M: A cluster of Drosophila homeobox genes involved in mesoderm differentiation programs. Bioessays. 2001, 23: 125-33.View ArticlePubMedGoogle Scholar
  40. Mizuhara E, Nakatani T, Minaki Y, Sakamoto Y, Ono Y: Corl1, a novel neuronal lineage-specific transcriptional corepressor for the homeodomain transcription factor Lbx1. J Biol Chem. 2005, 280: 3645-55.View ArticlePubMedGoogle Scholar
  41. Nabeshima Y, Hanaoka K, Hayasaka M, Esumi E, Li S, Nonaka I, Nabeshima Y: Myogenin gene disruption results in perinatal lethality because of severe muscle defect. Nature. 1993, 364: 532-5.View ArticlePubMedGoogle Scholar
  42. Potthoff MJ, Arnold MA, McAnally J, Richardson JA, Bassel-Duby R, Olson EN: Regulation of skeletal muscle sarcomere integrity and postnatal muscle function by Mef2c. Mol Cell Biol. 2007, 27: 8143-51.PubMed CentralView ArticlePubMedGoogle Scholar
  43. Lu J, Webb R, Richardson JA, Olson EN: MyoR: a muscle-restricted basic helix-loop-helix transcription factor that antagonizes the actions of MyoD. Proc Natl Acad Sci USA. 1999, 96: 552-7.PubMed CentralView ArticlePubMedGoogle Scholar
  44. Davis CA, Haberland M, Arnold MA, Sutherland LB, McDonald OG, Richardson JA, Childs G, Harris S, Owens GK, Olson EN: PRISM/PRDM6, a transcriptional repressor that promotes the proliferative gene program in smooth muscle cells. Mol Cell Biol. 2006, 26: 2626-36.PubMed CentralView ArticlePubMedGoogle Scholar
  45. Olson EN, Perry M, Schulz RA: Regulation of muscle differentiation by the MEF2 family of MADS box transcription factors. Dev Biol. 1995, 172: 2-14.View ArticlePubMedGoogle Scholar
  46. Westerfield M: The Zebrafish Book: A Guide for the Laboratory Use of Zebrafish (Brachydanio rerio). 2007, Eugene, OR: Univ. of Oregon PressGoogle Scholar
  47. Kimmel CB, Ballard WW, Kimmel SR, Ullmann B, Schilling TF: Stages of embryonic development of the zebrafish. Dev Dyn. 1995, 203: 253-310.View ArticlePubMedGoogle Scholar
  48. Peng G, Westerfield M: Lhx5 promotes forebrain development and activates transcription of secreted Wnt antagonists. Development. 2006, 133: 3191-200.View ArticlePubMedGoogle Scholar
  49. Westerfield M: The Zebrafish Book: A Guide for the Laboratory Use of Zebrafish (Brachydanio rerio). 2000, Eugene, OR: Univ. of Oregon PressGoogle Scholar
  50. Schulte-Merker S, van Eeden FJ, Halpern ME, Kimmel CB, Nusslein-Volhard C: no tail (ntl) is the zebrafish homologue of the mouse T (Brachyury) gene. Development. 1994, 120: 1009-15.PubMedGoogle Scholar
  51. Westerfield M: The Zebrafish Book: A Guide for the Laboratory Use of Zebrafish (Brachydanio rerio). 1993, Eugene, OR: Univ. of Oregon PressGoogle Scholar
  52. Au Y: The muscle ultrastructure: a structural perspective of the sarcomere. Cell Mol Life Sci. 2004, 61: 3016-33.View ArticlePubMedGoogle Scholar

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© Ochi and Westerfield; licensee BioMed Central Ltd. 2009

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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