Age- and calorie-independent life span extension from dietary restriction by bacterial deprivation in Caenorhabditis elegans

Table 1 BD increases life span independent of the age at which it is initiated.

Cohort	Number of animals	Mean Survival After Transfer	Mean Control Fed Survival After Transfer	p-value
CF	30	-	23.6 ± 0.7	-
Day 4 BD	110	37.6 ± 0.5	23.6 ± 0.7	1.4 × 10^-15
Day 8 BD	79	38.6 ± 0.5	23.6 ± 0.7	2.1 × 10^-15
Day 14 BD	158	38.1 ± 0.4	23.6 ± 0.7	4.8 × 10^-17
Day 20 BD	112	35.3 ± 0.7	25.0 ± 0.7	9.1 × 10^-8
Day 24 BD	52	35.2 ± 0.8	29.7 ± 1.2	0.01

'Mean survival after transfer' is the average life span of animals in each group that were alive at the time the cohort was switched from control fed (CF) to bacterial deprivation (BD). 'Mean control fed survival after transfer' is the average life span of control fed animals that were alive at the time the corresponding cohort was switched to BD. Survival data are shown as mean ± standard error. P-values were calculated by the Wilcoxon Rank-Sum test using the MATLAB 'ranksum' function.

ISSN: 1471-213X