The RGS gene loco is essential for male reproductive system differentiation in Drosophila melanogaster

Table 2 Complementation of male semi-sterile lines with deficiency lines

Cross	Total Eggs	Total Larvae	% viability	Chi²
OrR	217	214	98.6	/
loco ³¹⁸ /OrR	299	273	92.3	0.32 NS
loco ³⁵⁸ /OrR	237	217	91.6	0.33 NS
loco ³⁷⁰ /OrR	240	223	92.9	0.22 NS
loco ³⁸⁷ /OrR	313	285	91.1	0.51 NS
OrR/Df15	435	208	47.8	45.25 S
OrR/Df17	407	332	81.6	3.46 NS
OrR/loco ^Δ13	275	249	90.5	0.49 NS
loco ³¹⁸ /Df15	366	121	33.1	5.86 NS
loco ³¹⁸ /Df17	791	459	58.0	17.67 S
loco ³¹⁸ /loco ^Δ13	1062	693	65.3	24.70 S
loco ³⁵⁸ /Df15	259	112	43.2	0.31 NS
loco ³⁵⁸ /Df17	368	280	76.1	0.39 NS
loco ³⁵⁸ /loco ^Δ13	228	131	57.5	9.94 S
loco ³⁷⁰ /Df15	250	76	30.4	6.90 S
loco ³⁷⁰ /Df17	403	284	70.5	1.98 NS
loco ³⁷⁰ /loco ^Δ13	731	491	67.2	14.23 S
loco ³⁸⁷ /Df15	755	226	29.9	22.06 S
loco ³⁸⁷ /Df17	569	368	64.7	6.75 S
loco ³⁸⁷ /loco ^Δ13	873	570	65.3	35.65 S

Heterozygous flies, containing the loco mutation and the deficiency chromosome (Df(3R)15CE1, Df(3R)17D1, loco^Δ13) or the OrR, chromosome, were crossed with OrR. The ratio of eggs laid and eggs hatched is represented as a percentage. Significance was calculated by calculating Chi² values between heterozygous mutant males and OrR, and the hemizygous mutants to the heterozygous deficiency lines. Chi² > 6.64 suggests the two lines are closely linked. S: significant, NS: not significant.

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