Figure 4

Identification of the various loco transcripts in pupae and testes. A: Transcripts loco c1, loco c2, loco c3, and loco c5 were detected in early pupae. B: The transcripts loco c1, loco c2, and loco c3 were detected in late staged pupae, however loco c5 was not. C: Expression of loco c1 was detected in loco³¹⁸as was the 5' end of loco c2. However the 3'end of loco c2 (3' c2) was not detected in loco³¹⁸as only the non-specific band was produced (Sequencing and alignment showed this band to be CaBP1). Expression of loco c3 was also absent in loco³¹⁸, however expression of loco c5 remained unaffected. D: Only expression of loco c3 was lost in the pupae of the mutant line loco³⁵⁸. E: All loco transcripts were detected in the pupae of the mutant line loco³⁸⁷. F: Expression of loco c3 was lost in the testes of the mutant line loco³⁵⁸. The true PCR product of loco c2 is marked with an asterisk.