- Research article
- Open Access
Nlz1/Znf703 acts as a repressor of transcription
- Mako Nakamura†1, 3,
- Seong-Kyu Choe†1,
- Alexander P Runko1, 4,
- Paul D Gardner2 and
- Charles G Sagerström1Email author
© Nakamura et al; licensee BioMed Central Ltd. 2008
- Received: 28 August 2008
- Accepted: 12 November 2008
- Published: 12 November 2008
Members of the NET subfamily of zinc-finger proteins are related to the Sp-family of transcription factors and are required during embryogenesis. In particular, Nlz1/Znf703 and Nlz2/Znf503 are required for formation of rhombomere 4 of the vertebrate hindbrain. While NET family proteins have been hypothesized to regulate transcription, it remains unclear if they function as activators or repressors of transcription.
Here we demonstrate that Nlz proteins repress transcription both in cell lines and in developing zebrafish embryos. We first use standard cell culture-based reporter assays to demonstrate that Nlz1/Znf703 represses transcription of a luciferase reporter in four different cell lines. Structure-function analyses and pharmacological inhibition further reveal that Nlz1-mediated repression requires histone deacetylase activity. We next generate a stable transgenic zebrafish reporter line to demonstrate that Nlz1 promotes histone deacetylation at the transgenic promoter and repression of transgene expression during embryogenesis. Lastly, taking a genetic approach we find that endogenous Nlz proteins are required for formation of hindbrain rhombomere 4 during zebrafish embryogenesis by repressing expression of non-rhombomere 4 genes.
We conclude that Nlz1/Znf703 acts as a repressor of transcription and hypothesize that other NET family members function in a similar manner.
- Luciferase Reporter
- Zebrafish Embryo
- Fgf3 Expression
- C2H2 Zinc Finger
- Antisense Morpholino Oligos
The zebrafish nlz1/znf703 and nlz2/znf503 genes are closely related to the Drosophila noc and elbow genes, the C. elegans tlp-1 gene and several mammalian genes [1–10]. Together these genes make up a subclass (the NET family) that is related to the Sp family of zinc finger transcription factors (reviewed in ). Specifically, NET family proteins share three sequence motifs (a 'buttonhead box', an 'Sp motif' and a C2H2 zinc finger) with Sp family proteins. While Sp family transcription factors (Sp1–Sp8) are sequence-specific transcription factors acting as activators or repressors depending on the cellular context [12–22], it is unclear if NET proteins play a similar role. For instance, Sp proteins contain three C2H2 zinc fingers that bind DNA, but NET proteins contain only one zinc finger and this may not be sufficient to bind DNA ([23, 24]; reviewed in ). Further, the single C2H2 finger in NET proteins is atypical and is unlikely to bind DNA directly (reviewed in ). Accordingly, direct sequence-specific DNA-binding has not been reported for any NET family proteins. Thus, while Sp proteins function as transcription factors, the biochemical activity of NET family proteins is unclear.
Though NET proteins may not bind DNA directly, the available evidence points to their having a role in transcriptional regulation. For instance, Elbow, TLP-1, Nlz1 and Nlz2 are located in the nucleus [2, 3, 6, 7] and Nlz1 must be nuclearly localized to be fully active . Furthermore, gain- and loss-of-function experiments suggest that NET family proteins modulate gene expression during embryogenesis. In particular, expression of spalt, a marker of the dorsal tracheal trunk in Drosophila, is abolished in response to ectopic expression of elbow and is expanded in elbow mutants . Similarly, ectopic expression of nlz1 or nlz2 disrupts krox20 expression in rhombomere 3 of the zebrafish hindbrain and leads to an expansion of hoxb1a expression from rhombomere 4. Disruption of nlz function has the opposite effect, leading to loss of hoxb1a expression and expansion of krox20 expression [2–4].
While NET proteins appear likely to regulate transcription, it is unclear if they function as activators or repressors or both, as is the case for SP-family proteins. Drosophila Elbow is reported to interact with the transcriptional repressor Groucho  and both Nlz1 and Nlz2 interact with Groucho as well as with several histone deacetylase (HDAC) co-repressors [2, 3]. Against this background, we have hypothesized that NET family proteins act as repressors of transcription during embryogenesis , but there is no direct evidence for NET family proteins repressing transcription and it remains plausible that they can function as both activators and repressors.
Here we demonstrate that Nlz proteins repress transcription both in cell lines and in developing zebrafish embryos. We first use standard cell culture-based reporter assays to demonstrate that Nlz1 represses transcription of a luciferase reporter in four different cell lines. We find that this repression requires a domain of Nlz1 that includes the HDAC binding site and that it is blocked by the HDAC inhibitor Trichostatin A, indicating that Nlz1-mediated repression requires HDACs. Next, we generate a stable transgenic zebrafish reporter line and use it to demonstrate that Nlz1 represses a luciferase reporter in the developing embryo. By adapting chromatin immunoprecipitation assays to zebrafish embryos, we further demonstrate that this repression is accompanied by histone deacetylation at the transgene promoter, again consistent with Nlz-mediated repression requiring HDACs. Lastly, we take a genetic approach to examine the function of endogenous Nlz proteins. We find that Nlz proteins are required for formation of hindbrain rhombomere 4 because they repress expression of non-rhombomere 4 genes. We do not find any evidence for Nlz proteins being required as activator during hindbrain formation. We conclude that Nlz proteins act as repressors of transcription and hypothesize that other NET family members function in a similar manner.
The UAS-SV40:Luciferase plasmid was generated by inserting oligonucleotides containing three UAS sites upstream of the SV40 promoter in the pGL3 plasmid (Promega). All Nlz1-GAL4DBD fusion constructs were Myc-tagged at the N-teminus and generated by PCR amplifying the appropriate regions of Nlz1 and subcloning them upstream of the GAL4DBD domain in the pCS2MT expression vector.
Cell culture, transfection and reporter assay
HeLa, NIH3T3 and SN17 cells were maintained in DMEM containing 10% FBS at 37°C and ZF4 cells were maintained in 1:1 DMEM/F10 with 10% FBS at 28°C. For transfections, 2.5 × 105 cells were plated in a single well of 6-well plates. The FuGENE6 (Roche) system was used for transfection of HeLa, NIH3T3 and SN17 cells and Effectene (Qiagen) for transfection of ZF4 cells. Luciferase reporter plasmid (1.5 μg, except for ZF4 which was 0.25 μg) was co-transfected with β-galactosidase plasmid (0.5 μg, except for ZF4 which was 0.083 μg) and 20 ng, 100 ng or 500 ng of Gal4DBD or Nlz1-Gal4DBD expression vector (except for ZF4, which was 0.0033, 0.017 and 0.083 ng). The corresponding empty vector was used to adjust the total amount of DNA to 2.5 μg per well (except ZF4, which was 0.42 μg). For TSA experiments, transfected cells were treated with DMSO, 0.1 μM or 0.3 μM TSA starting 24 hrs after transfection. Cells were harvested 48 hrs after transfection. Cells were lysed and analyzed for luciferase and β-galactosidase activities followed by normalization of luciferase activity based on β-galactosidase activity.
Samples were separated on 12.5% SDS PAGE and transferred to a PVDF membrane. The membrane was incubated in TBS-T Blocking buffer (Tris-Tween plus 5% BSA) for one hour, then with 1 μg/ml anti-Myc antibody in TBS-T 1% BSA at 4°C O/N with gentle shaking. The membrane was then washed with TBS-T, incubated with 1:2000 anti-mouse HRT antibody in 5% skim milk for one hour with gentle agitation, washed again with TBS-T and developed with Supersignal Ultra reagent (Pierce).
Generation of transgenic UAS-SV40:Luciferase reporter line
The UAS-SV40:Luciferase vector was linearized with NotI. DNA (50 pg) was injected into embryos at the 1-cell stage. Injected embryos were raised to adulthood (F0 generation) and out-crossed to wild type fish. Embryos from each cross were pooled and assayed by PCR (Forward oligo:5-TGC ATC TCA ATT AGT CAG CCA CCA TAG-3; reverse oligo:5-ACC GGA ATG CCA AGC TTT TTG-3) for presence of the transgene. F0 carriers were then out-crossed and the offspring raised to adulthood (F1 generation). F1 fish were genotyped by PCR of fin clip biopsies. Confirmed F1 carriers were used to produce embryos for reporter experiments.
Promoter assay using TG(UAS-SV40:Luciferase) embryos
F1 TG(UAS-SV40:Luciferase) heterozygous carriers were out crossed and the F2 embryos injected with 400 pg mRNA encoding Gal4DBD or Nlz1-Gal4DBD at the 2–4 cell stage. Injected embryos were raised 11–12 hours (to early somitogenesis stage) and homogenized in 100 μl luciferase lysis buffer (Promega). Each sample was centrifuged and 20 μl of supernatant was used for luciferase assays (Promega). Injection of equivalent amounts of mRNA was confirmed by quantitative PCR.
ChIPs were performed as described previously [26, 27] with modifications to allow dissociation of zebrafish embryos into single cells. Specifically, 30 zebrafish embryos were collected at 16hpf, dechorionated and incubated with 40 units of collagenase (Invitrogen) in PBS for 30 minutes at 37°C prior to cross-linking. 1 μl anti-acetyl histone H4 antibody (Upstate) was used for the ChIP. The purified DNA was dissolved in 50 μl Tris-EDTA and 2 μl was used for quantitative PCR. 1% of chromatin taken before immunoprecipitation was used as input. Quantitative PCR was performed with 7.5 μl of QuantiTect SYBR Green PCR mix (Qiagen), 5.5 μl of dH2O and 2 μl of DNA with 0.67 μM of the primers (Forward: AAGAAAGGCCCGGCGCCATTCTATCCGCTG, Reverse: GAAGTACTCAGCGTAAGTGATGTCCACCTC). PCR amplification was detected and normalized as a ratio to the input for each sample by using a DNA Engine Opticon (MJ Research).
Zebrafish lines and in situ hybridization
vhnf1hi2169 mutants were obtained from N. Hopkins . Nlz loss-of-function embryos were prepared by microinjecting anti-sense morpholino oligos targeting nlz1 and nlz2 as reported previously . The whole-mount in situ hybridization protocol and probes were described previously [2, 3].
Nlz1 represses transcription in a diverse set of cell lines
The HDAC binding domain in Nlz1 is required for repression
To test which domains of Nlz1 are required for repression in the reporter assay, we fused a series of Nlz1 deletion constructs to the GAL4DBD (Fig. 2A). Western blotting experiments demonstrate that all constructs are well expressed upon transfection into HeLa cells (Fig. 2B). Additionally, the GAL4DBD contains a nuclear localization signal ensuring that all fusion proteins localize to the nucleus (not shown). We next examined each of the deletion constructs for its ability to repress the UAS-SV40:Luciferase reporter in HeLa cells (Fig. 2A). As expected, we find that intact Nlz1-GAL4DBD represses Luciferase expression in a robust, dose-dependent manner. Further, deleting the buttonhead box (Δ385–408) has a minimal effect on HDAC binding (Fig. 2A; ) and this construct retains repressor activity (~2 fold less repression than full-length Nlz1; Fig. 2A). In contrast, Nlz1 constructs lacking the HDAC-interaction domain (Δ385–460, Δ408–589) repress the reporter only weakly (~4–5 fold less repression than full-length Nlz1). Together these results demonstrate that the HDAC-interaction domain is required for optimal activity of Nlz1 and are consistent with Nlz1 recruiting HDACs to repress transcription. However, we note some residual repressor activity (~3 fold above GAL4DBD alone) in the two constructs that do not bind HDACs (Δ385–460 and Δ408–589) suggesting that Nlz1 may also repress transcription by a HDAC-independent mechanism.
Nlz family proteins share an N-terminal sequence motif (S-P-L-A-L/M-L-A-A/Q-T-C) with Sp-family proteins (SP; Fig. 2A). While the function of this domain is not fully understood, it has been implicated in regulating the transcription activity of Sp1 . Further, although this motif is not involved in binding to co-repressors, we have shown that it is required for Nlz1 function in zebrafish embryos [2, 3]. We therefore tested a construct lacking the N-terminal 115 amino acids, including the Sp motif, in the reporter assay. We find that ΔN115Nlz1-GAL4DBD has robust repressor activity (Fig. 2A), indicating that this domain is not required for Nlz1-mediated repression in HeLa cells (see discussion).
Nlz1-mediated repression is sensitive to Trichostatin A
Nlz1 represses a UAS-SV40:Luciferase transgene in zebrafish embryos
Nlz proteins are required for rhombomere-restricted expression of gbx1 and vhnf1
Nlz proteins act as repressors in r4
Our results suggest that Nlz proteins are required to repress expression of vhnf1 and gbx1 in r4. Failure to repress these genes could account for the reported loss of hoxb1a expression in r4 of nlz- embryos. In particular, vhnf1 is known to repress hoxb1a expression [28, 32–34] and expression of vhnf1 in r4 of nlz- embryos might therefore suppress hoxb1a expression.
We took a genetic approach to determine if Nlz proteins are required in r4 because they repress vhnf1 expression. In particular, if hoxb1a expression in r4 requires that Nlz proteins repress expression of vhnf1, then removing vhnf1 function on a nlz loss-of-function background might restore hoxb1a expression. Put more generally, if expression of hoxb1a requires repression of a repressor, then removing both repressors should restore hoxb1a expression. As reported, hoxb1a expression is lost in nlz- embryos (Fig. 6J; ) by the 14 somite stage. Strikingly, we find that hoxb1a expression is robustly restored when vhnf1 function is removed on the nlz- background (Fig. 6L). We also note the same behavior for fgf3 expression, as fgf3 expression is lost in r4 of nlz- embryos (Fig. 6R) and restored to nlz-/vhnf1- embryos (Fig. 6T). Thus, when vhnf1 is missing, Nlz proteins are not required for hoxb1a or fgf3 expression – consistent with Nlz proteins acting to repress vhnf1 expression. This finding also excludes the possibility that Nlz proteins are required to activate hoxb1a or fgf3 expression in r4. Notably, since vhnf1 function is lacking in r5/r6 of nlz-/vhnf1- embryos, krox20 expression is lost from r5 and r4 expression extends into r5/r6 in these embryos, as also observed in vhnf1- embryos (Fig. 6K, S; [28, 33, 34]). Lastly, nlz-/vhnf1- embryos display expanded r3 krox20 expression, perhaps as a result of a failure to repress gbx1 expression. In summary, our genetic analysis supports a role for Nlz proteins in repressing expression of non-r4 genes.
Rhombomere 4 has been postulated as an important signaling center required for segmentation of the hindbrain [36, 37]. Yet, while nlz- embryos lack r4, the adjacent r3 and r5 territories appear relatively intact (Fig. 6J, R; ). We therefore examined earlier stage embryos and find that hoxb1a is robustly expressed in nlz- embryos at the tailbud stage (Fig. 6N). Similarly, although fgf3 expression is weak in tailbud stage embryos, we routinely observe fgf3 expression in the region corresponding to r4 of nlz- embryos (arrow in Fig. 6V). Thus, r4 gene expression appears to be initiated in nlz- embryos, further supporting our hypothesis that Nlz proteins are not required to induce gene expression in r4, but to repress expression of non-r4 genes.
Based on their sequence relationship to Sp transcription factors and their roles during embryonic development, Nlz proteins and other NET family members have been postulated to regulate transcription. In particular, Nlz proteins share several domains, including a C2H2 zinc finger, with Sp transcription factors and Nlz proteins localize to the nucleus. Additionally, mis-expression of nlz1 or nlz2 blocks expression of krox20 in rhombomere 3 of the zebrafish hindbrain and permits expansion of r4 gene expression . Conversely, disruption of Nlz function leads to expansion of krox20 expression accompanied by loss of hoxb1a expression, albeit to various degrees [2–4]. Here we use a combination of biochemical and genetic approaches to demonstrate that Nlz proteins function as repressors, at least in part by recruiting HDACs, and that they act to repress non-r4 genes during zebrafish hindbrain development.
Biochemical activity of Nlz proteins
Our results demonstrate that Nlz proteins repress transcription of a luciferase reporter gene both in cell lines and in zebrafish embryos. Our structure-function analysis revealed that Nlz proteins require a region containing the HDAC interaction domain for optimal repression activity. In addition, Nlz1-mediated repression in cell lines is blocked by the HDAC antagonist Trichostatin A (TSA) and Nlz1 expression promotes histone deacetylation at a transgenic promoter in zebrafish embryos. These findings suggest that Nlz proteins repress transcription by recruiting HDACs to target promoters. Notably, HDACs are broadly expressed in zebrafish embryos , suggesting that they are available for Nlz-mediated repression. Our results also indicate that Nlz proteins may repress transcription partly by an HDAC-independent mechanism. Specifically, we observe residual repressor activity for Nlz constructs that cannot bind HDACs and Nlz1 retains some repressor activity even in the presence of TSA. Although we do not presently know how this residual repressor effect is mediated, our finding that Nlz proteins repress transcription by recruiting HDACs represents an important advance in the understanding of Nlz function.
We have noted that NET family proteins are unlikely to bind DNA directly. These proteins may therefore need to be recruited to DNA by other DNA binding factors, but it is presently unclear how this may be achieved. A potential clue may be provided by our structure-function analysis, which revealed that the N-terminal SP domain (that is conserved between NET and Sp family proteins) is not required for Nlz-mediated repression in cell lines. This is in contrast to our previous analysis in zebrafish embryos, where we found that the Sp domain is required for optimal Nlz1 function . We note that these analyses differ such that our current report utilizes a Nlz1-GAL4DBD fusion protein – where Nlz1 is recruited to DNA via the GAL4 DNA binding domain – whereas our previous work utilized wild type Nlz1. Hence, it is possible that the Sp domain acts as a binding site for a protein that recruits Nlz1 to target promoters in the embryo, although this possibility awaits confirmation by the identification of proteins that bind the Sp motif. The role of this motif in Sp protein function is not clear and it has been ascribed several potential functions (e.g. regulation of protein stability), but there are reports that the Sp motif acts as a protein interaction site .
Lastly, while we have not detected any evidence that Nlz proteins can activate transcription, we cannot exclude the possibility that Nlz proteins function as activators in specific cellular contexts. Indeed, some Sp proteins are thought to function as activators or repressors depending on the availability of cofactors (reviewed in ). Since such an activator function might be obscured by the high basal activity of the SV40 promoter in our reporter construct, we have tested if Nlz1-GAL4DBD can activate transcription from a reporter construct containing a minimal β-globin promoter, but we have not observed activation of transcription (not shown).
Biological role of Nlz proteins
Disruption of Nlz function leads to loss of hoxb1a expression in r4 and expansion of krox20 expression from r3 and r5 of the hindbrain. While hoxb1a is one of the earliest-acting genes in r4, there are several genes acting earlier than krox20 in r3 and r5. In particular, vhnf1 is reported to act very early in the formation of r5 and to be required for krox20 expression in r5 [28, 33, 34], while gbx1 acts early in r1-r3 . Hence, we examined expression of vhnf1 and gbx1 in Nlz loss-of-function embryos and found that expression of these genes expands into r4 – indicating that gbx1 and vhnf1 expression is normally repressed in r4 by Nlz proteins. Since vHnf1 represses hoxb1a expression [28, 32–34], we postulated that Nlz proteins might function to repress vhnf1 expression in r4 – thereby preventing vhnf1-mediated repression of hoxb1a expression. In support of this hypothesis we find that removing vhnf1 function on a Nlz loss-of-function background restores hoxb1a expression, indicating that Nlz proteins are required to repress vhnf1 expression in cells that will form r4. At present, we do not know if this repression is direct (i.e. whether Nlz acts at the vhnf1 promoter to repress transcription) or if there are additional intervening steps. This issue is complicated by the fact that Nlz proteins may need to be recruited to target promoters by as yet unknown DNA binding proteins (see above), further underscoring that an important next step will be to identify Nlz-binding proteins. We also note that gbx1 expression expands throughout the caudal embryo in the absence of Nlz function. This indicates that Nlz may repress gene expression also outside r4, consistent with the broad expression of nlz genes at early stages of development.
We demonstrate that Nlz1 represses transcription of a luciferase reporter in four different cell lines and that this repression requires HDACs. We generate a transgenic zebrafish reporter line and demonstrate that Nlz1 represses transcription and promotes histone deacetylation at the transgene promoter in developing embryos, consistent with Nlz1-mediated repression requiring HDACs also in vivo. Lastly, we take a genetic approach to demonstrate that Nlz proteins are required as repressors, but not activators, of transcription in formation of hindbrain rhombomere 4 during zebrafish embryogenesis. We conclude that Nlz proteins act as repressors of transcription and hypothesize that other NET family members function in a similar manner. Our results also demonstrate the utility of stable transgenic zebrafish-based reporter assays as a complement to cell culture-based reporter assays.
The authors thank Dr Scot Wolfe for helpful discussions about zinc finger proteins and Letitiah Etheridge for expert assistance in preparation of the TG(UAS-SV40:Luciferase) line. We are grateful to Yasuyuki Ohkawa and Anthony Imbalzano for assistance with the ChIP assays ant Cecilia Moens for fgf plasmids. We acknowledge Dr Nancy Hopkins for providing the vhnf1hi2169 strain. This work was funded by grants FY04-105 and FY07-430 from the March of Dimes.
- McGlinn E, van Bueren KL, Fiorenza S, Mo R, Poh AM, Forrest A, Soares MB, Bonaldo Mde F, Grimmond S, Hui CC, Wainwright B, Wicking C: Pax9 and Jagged1 act downstream of Gli3 in vertebrate limb development. Mech Dev. 2005, 122: 1218-33. 10.1016/j.mod.2005.06.012.View ArticlePubMedGoogle Scholar
- Runko AP, Sagerstrom CG: Nlz belongs to a family of zinc-finger-containing repressors and controls segmental gene expression in the zebrafish hindbrain. Developmental Biology. 2003, 262: 254-267. 10.1016/S0012-1606(03)00388-9.View ArticlePubMedGoogle Scholar
- Runko AP, Sagerstrom CG: Isolation of nlz2 and characterization of essential domains in Nlz family proteins. J Biol Chem. 2004, 279: 11917-25. 10.1074/jbc.M310076200.View ArticlePubMedGoogle Scholar
- Hoyle J, Tang YP, Wiellette EL, Wardle FC, Sive H: nlz gene family is required for hindbrain patterning in the zebrafish. Dev Dyn. 2004, 229: 835-46. 10.1002/dvdy.20001.View ArticlePubMedGoogle Scholar
- Cheah PY, Meng YB, Yang X, Kimbrell D, Ashburner M, Chia W: The Drosophila l(2)35Ba/nocA gene encodes a putative Zn finger protein involved in the development of the embryonic brain and the adult ocellar structures. Molecular and Cellular Biology. 1994, 14: 1487-1499.View ArticlePubMed CentralPubMedGoogle Scholar
- Dorfman R, Glazer L, Weihe U, Wernet MF, Shilo BZ: Elbow and Noc define a family of zinc finger proteins controlling morphogenesis of specific tracheal branches. Development. 2002, 129: 3585-96.PubMedGoogle Scholar
- Zhao X, Yang Y, Fitch DH, Herman MA: TLP-1 is an asymmetric cell fate determinant that responds to Wnt signals and controls male tail tip morphogenesis in C. elegans. Development. 2002, 129: 1497-508.PubMedGoogle Scholar
- Weihe U, Dorfman R, Wernet MF, Cohen SM, Milan M: Proximodistal subdivision of Drosophila legs and wings: the elbow-no ocelli gene complex. Development. 2004, 131: 767-74. 10.1242/dev.00979.View ArticlePubMedGoogle Scholar
- Andreazzoli M, Broccoli V, Dawid IB: Cloning and expression of noz1, a zebrafish zinc finger gene related to Drosophila nocA. Mech Dev. 2001, 104: 117-20. 10.1016/S0925-4773(01)00359-8.View ArticlePubMedGoogle Scholar
- Chang CW, Tsai CW, Wang HF, Tsai HC, Chen HY, Tsai TF, Takahashi H, Li HY, Fann MJ, Yang CW, Hayashizaki Y, Saito T, Liu FC: Identification of a developmentally regulated striatum-enriched zinc-finger gene, Nolz-1, in the mammalian brain. Proc Natl Acad Sci USA. 2004, 101: 2613-8. 10.1073/pnas.0308645100.View ArticlePubMed CentralPubMedGoogle Scholar
- Nakamura M, Runko AP, Sagerstrom CG: A novel subfamily of zinc finger genes involved in embryonic development. J Cell Biochemistry. 2004, 93: 887-895. 10.1002/jcb.20255.View ArticleGoogle Scholar
- Wong WK, Chen K, Shih JC: Regulation of human monoamine oxidase B gene by Sp1 and Sp3. Mol Pharmacol. 2001, 59: 852-9.PubMedGoogle Scholar
- Kwon HS, Kim MS, Edenberg HJ, Hur MW: Sp3 and Sp4 can repress transcription by competing with Sp1 for the core cis-elements on the human ADH5/FDH minimal promoter. J Biol Chem. 1999, 274: 20-8. 10.1074/jbc.274.1.20.View ArticlePubMedGoogle Scholar
- Kennett SB, Moorefield KS, Horowitz JM: Sp3 represses gene expression via the titration of promoter-specific transcription factors. J Biol Chem. 2002, 277: 9780-9. 10.1074/jbc.M108661200.View ArticlePubMedGoogle Scholar
- Ihn H, Trojanowska M: Sp3 is a transcriptional activator of the human alpha2(I) collagen gene. Nucleic Acids Res. 1997, 25: 3712-7. 10.1093/nar/25.18.3712.View ArticlePubMed CentralPubMedGoogle Scholar
- Hagen G, Muller S, Beato M, Suske G: Sp1-mediated transcriptional activation is repressed by Sp3. Embo J. 1994, 13: 3843-51.PubMed CentralPubMedGoogle Scholar
- Phan D, Cheng CJ, Galfione M, Vakar-Lopez F, Tunstead J, Thompson NE, Burgess RR, Najjar SM, Yu-Lee LY, Lin SH: Identification of Sp2 as a transcriptional repressor of carcinoembryonic antigen-related cell adhesion molecule 1 in tumorigenesis. Cancer Res. 2004, 64: 3072-8. 10.1158/0008-5472.CAN-03-3730.View ArticlePubMedGoogle Scholar
- Bakovic M, Waite KA, Vance DE: Functional significance of Sp1, Sp2, and Sp3 transcription factors in regulation of the murine CTP:phosphocholine cytidylyltransferase alpha promoter. J Lipid Res. 2000, 41: 583-94.PubMedGoogle Scholar
- Marin M, Karis A, Visser P, Grosveld F, Philipsen S: Transcription factor Sp1 is essential for early embryonic development but dispensable for cell growth and differentiation. Cell. 1997, 89: 619-28. 10.1016/S0092-8674(00)80243-3.View ArticlePubMedGoogle Scholar
- Saffer JD, Jackson SP, Annarella MB: Developmental expression of Sp1 in the mouse. Mol Cell Biol. 1991, 11: 2189-99.View ArticlePubMed CentralPubMedGoogle Scholar
- Courey AJ, Tjian R: Analysis of Sp1 in vivo reveals multiple transcriptional domains, including a novel glutamine-rich activation motif. Cell. 1988, 55: 887-98. 10.1016/0092-8674(88)90144-4.View ArticlePubMedGoogle Scholar
- Courey AJ, Holtzman DA, Jackson SP, Tjian R: Synergistic activation by the glutamine-rich domains of human transcription factor Sp1. Cell. 1989, 59: 827-36. 10.1016/0092-8674(89)90606-5.View ArticlePubMedGoogle Scholar
- Iuchi S: Three classes of C2H2 zinc finger proteins. Cell Mol Life Sci. 2001, 58: 625-35. 10.1007/PL00000885.View ArticlePubMedGoogle Scholar
- Berg J: Zinc Fingers and other metal-binding domains. J Biol Chem. 1990, 265: 6513-6516.PubMedGoogle Scholar
- Wolfe SA, Nekludova L, Pabo CO: DNA recognition by Cys2His2 zinc finger proteins. Annu Rev Biophys Biomol Struct. 2000, 29: 183-212. 10.1146/annurev.biophys.29.1.183.View ArticlePubMedGoogle Scholar
- Salma N, Xiao H, Mueller E, Imbalzano AN: Temporal recruitment of transcription factors and SWI/SNF chromatin-remodeling enzymes during adipogenic induction of the peroxisome proliferator-activated receptor gamma nuclear hormone receptor. Mol Cell Biol. 2004, 24: 4651-63. 10.1128/MCB.24.11.4651-4663.2004.View ArticlePubMed CentralPubMedGoogle Scholar
- de la Serna IL, Ohkawa Y, Berkes CA, Bergstrom DA, Dacwag CS, Tapscott SJ, Imbalzano AN: MyoD targets chromatin remodeling complexes to the myogenin locus prior to forming a stable DNA-bound complex. Mol Cell Biol. 2005, 25: 3997-4009. 10.1128/MCB.25.10.3997-4009.2005.View ArticlePubMed CentralPubMedGoogle Scholar
- Sun Z, Hopkins N: vhnf1, the MODY5 and familial GCKD-associated gene, regulates regional specification of the zebrafish gut, pronephros, and hindbrain. Genes Dev. 2001, 15: 3217-29. 10.1101/gad946701.View ArticlePubMed CentralPubMedGoogle Scholar
- Murata Y, Kim HG, Rogers KT, Udvadia AJ, Horowitz JM: Negative regulation of Sp1 trans-activation is correlated with the binding of cellular proteins to the amino terminus of the Sp1 trans- activation domain. J Biol Chem. 1994, 269: 20674-81.PubMedGoogle Scholar
- Yoshida M, Kijima M, Akita M, Beppu T: Potent and specific inhibition of mammalian histone deacetylase both in vivo and in vitro by trichostatin A. J Biol Chem. 1990, 265: 17174-9.PubMedGoogle Scholar
- Moens CB, Prince VE: Constructing the hindbrain: Insights from the zebrafish. Dev Dyn. 2002, 224: 1-17. 10.1002/dvdy.10086.View ArticlePubMedGoogle Scholar
- Choe S, Sagerstrom CG: Paralog group 1 hox genes regulate rhombomere 5/6 expression of vnhf1, a repressor of rostral hindbrain fates, in a meis-dependent manner. Developmental Biology. 2004, 271: 350-361. 10.1016/j.ydbio.2004.04.007.View ArticlePubMedGoogle Scholar
- Hernandez RE, Rikhof HA, Bachmann R, Moens CB: vhnf1 integrates global RA patterning and local FGF signals to direct posterior hindbrain development in zebrafish. Development. 2004, 131: 4511-20. 10.1242/dev.01297.View ArticlePubMedGoogle Scholar
- Wiellette EL, Sive H: vhnf1 and Fgf signals synergize to specify rhombomere identity in the zebrafish hindbrain. Development. 2003, 130: 3821-9. 10.1242/dev.00572.View ArticlePubMedGoogle Scholar
- Rhinn M, Lun K, Amores A, Yan YL, Postlethwait JH, Brand M: Cloning, expression and relationship of zebrafish gbx1 and gbx2 genes to Fgf signaling. Mech Dev. 2003, 120: 919-36. 10.1016/S0925-4773(03)00135-7.View ArticlePubMedGoogle Scholar
- Walshe J, Maroon H, McGonnell IM, Dickson C, Mason I: Establishment of Hindbrain Segmental Identity Requires Signaling by FGF3 and FGF8. Curr Biol. 2002, 12: 1117-23. 10.1016/S0960-9822(02)00899-0.View ArticlePubMedGoogle Scholar
- Maves L, Jackman W, Kimmel CB: FGF3 and FGF8 mediate a rhombomere 4 signaling activity in the zebrafish hindbrain. Development. 2002, 129: 3825-37.PubMedGoogle Scholar
- Cunliffe VT: Histone deacetylase 1 is required to repress Notch target gene expression during zebrafish neurogenesis and to maintain the production of motoneurones in response to hedgehog signalling. Development. 2004, 131: 2983-95. 10.1242/dev.01166.View ArticlePubMedGoogle Scholar
- Su K, Roos MD, Yang X, Han I, Paterson AJ, Kudlow JE: An N-terminal region of Sp1 targets its proteasome-dependent degradation in vitro. J Biol Chem. 1999, 274: 15194-202. 10.1074/jbc.274.21.15194.View ArticlePubMedGoogle Scholar
- Kaczynski J, Cook T, Urrutia R: Sp1- and Kruppel-like transcription factors. Genome Biol. 2003, 4: 206-10.1186/gb-2003-4-2-206.View ArticlePubMed CentralPubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.