Figure 8
From: Sex-specific DoublesexM expression in subsets of Drosophilasomatic gonad cells
DSXM expression does not require germ cells nor EYA or TJ. (A-D) Agametic testis of stage 15 males from gs(1)N26 mothers. Immunofluorescence using (A) anti-DSXM, (B) anti-TJ, and (C) anti-VASA. (D) Merged images A-C. (E-H) Isolated somatic gonadal precursors and germ cells formed in homozygous eya mutants. Immunofluorescence using (E) anti-DSXM, (F) anti-TJ, and (G) anti-VASA. (H) Merged images E-G. (I-L) Expression in tjeomutant testis revealed with immunofluorescence using (I) anti-DSXM, (J) anti-TJ, and (K) anti-VASA. (L) Merged images I-K. Expression of the non-functional truncated TJ protein in tjeomutant embryos is diffuse within the somatic gonadal precursors (J). The scale bars = 10 μm. Anterior is to the left. Secondary antibodies were: (A, E, I) biotin-coupled goat anti-rat and TSA, (B) Alexa 647 goat anti-guinea pig, (C) Alexa 488 goat anti-rabbit, (F, J) Alexa 488 goat anti-guinea pig, and (G, K) Cy5 goat anti-rabbit.