Figure 5
From: Sex-specific DoublesexM expression in subsets of Drosophilasomatic gonad cells
DSXM is not expressed in all somatic testis cells. (A-D) stage 17 testis immunofluorescence using (A) anti-DSXM, (B) anti-SOX100B, and (C) anti-VASA. (D) Merged images A-C. (E-H) stage 17 testis immunofluorescence (imaged in a focal plane with the hub) using (E) anti-DSXM, (F) anti-FAS III, and (G) anti-VASA. (H) Merged images E-G. The scale bars = 10 μm. Secondary antibodies were: (A, E) biotin-coupled goat anti-rat and TSA, (B) Alexa 488 goat anti-rabbit, (C) Alexa 647 goat anti-chicken, (F) Alexa 647 goat anti-mouse, and (G) Cy5 goat anti-rabbit.