Figure 1

dsx^mand DSX^M in embryos. (A) RT-PCR of dsx^mfrom sex sorted embryos of ages 3–10 hours; 10–16 hours and 16–22 hours. (B) β3-tubulin amplification control. (C) Cartoon of dsx transcripts. The positions of the primers used for the amplification of the male-specific dsx^mproducts are marked (arrows). (D, F, H) Anti-DSX^M and (E, G, I) anti-VASA immunofluorescence in (D, E) female, (F, G) male, and (H, I) dsx^- embryos (Df(3R)dsx¹⁵/In(3R)dsx²³). Images in each row are from the same confocal section of an embryo. Because we used GFP to distinguish homozygous dsx^- from balancer and heterozygous control embryos, embryos in H, I were not sex sorted. However, we never observed DSX^M staining in dsx^- embryos, 50% of which were male. Secondary antibody for anti-DSX^M was biotin-coupled goat anti-rat with tyramide signal amplification, and secondary for anti-VASA was Cy5 goat anti-rabbit. Scale bar = 10 μm.