- Research article
- Open Access
A genome-wide in situhybridization map of RNA-binding proteins reveals anatomically restricted expression in the developing mouse brain
- Adrienne E McKee†1, 2,
- Emmanuel Minet†2, 3,
- Charlene Stern2,
- Shervin Riahi2,
- Charles D Stiles2, 4 and
- Pamela A Silver1, 2Email author
© McKee et al; licensee BioMed Central Ltd. 2005
- Received: 06 May 2005
- Accepted: 20 July 2005
- Published: 20 July 2005
In eukaryotic cells, RNA-binding proteins (RBPs) contribute to gene expression by regulating the form, abundance, and stability of both coding and non-coding RNA. In the vertebrate brain, RBPs account for many distinctive features of RNA processing such as activity-dependent transcript localization and localized protein synthesis. Several RBPs with activities that are important for the proper function of adult brain have been identified, but how many RBPs exist and where these genes are expressed in the developing brain is uncharacterized.
Here we describe a comprehensive catalogue of the unique RBPs encoded in the mouse genome and provide an online database of RBP expression in developing brain. We identified 380 putative RBPs in the mouse genome. Using in situ hybridization, we visualized the expression of 323 of these RBP genes in the brains of developing mice at embryonic day 13.5, when critical fate choice decisions are made and at P0, when major structural components of the adult brain are apparent. We demonstrate i) that 16 of the 323 RBPs examined show neural-specific expression at the stages we examined, and ii) that a far larger subset (221) shows regionally restricted expression in the brain. Of the regionally restricted RBPs, we describe one group that is preferentially expressed in the E13.5 ventricular areas and a second group that shows spatially restricted expression in post-mitotic regions of the embryonic brain. Additionally, we find a subset of RBPs that share the same complex pattern of expression, in proliferating regions of the embryonic and postnatal NS and peripheral tissues.
Our data show that, in contrast to their proposed ubiquitous involvement in gene regulation, most RBPs are not uniformly expressed. Here we demonstrate the region-specific expression of RBPs in proliferating vs. post-mitotic brain regions as well as cell-type-specific RBP expression. We identify uncharacterized RBPs that exhibit neural-specific expression as well as novel RBPs that show expression in non-neural tissues. The data presented here and in an online database provide a visual filter for the functional analysis of individual RBPs.
- Inner Nuclear Layer
- Restricted Expression
- Develop Mouse Brain
- Localize Protein Synthesis
- External Granule Layer
The ordered production and differentiation of cell types that occurs during nervous system (NS) development relies upon tightly regulated gene expression. In neural cells, spatial and temporal gene regulation occurs through both transcriptional and post-transcriptional mechanisms. While the transcriptional networks that direct neural cell fate and govern cell shape, position, and connectivity have been well studied [1–3], the post-transcriptional influences on neural development and gene expression are less well understood.
At the core of post-transcriptional gene regulation are RNA-binding proteins (RBPs). Proteins containing canonical RNA-binding domains (RBDs) are involved in numerous steps of nuclear and cytoplasmic RNA processing . Through mRNA capping, splicing, editing, polyadenylation and nonsense-mediated decay, RBPs modulate the diversity of transcribed genes [4–6]. RBPs also affect the processing of non-coding RNAs . Specific RBPs additionally enable asymmetric RNA distribution and translational regulation [8–10], two phenomena that are critical for affecting localized protein synthesis [11, 12].
The importance of post-transcriptional processing in NS gene regulation is underscored by functional examples of specific RBPs [13, 14]. For instance, the neuronal-specific factor Nova-1 regulates splicing of pre-mRNAs that encode components of inhibitory synapses . Mice lacking Nova-1 die postnatally due to aberrant regulation of apoptotic neuronal death . As a second example, RBPs encoded by the quaking and Musashi loci promote glial cell fate  and CNS stem cell self-renewal  by stabilizing transcripts involved in cell differentiation. Thirdly, the fragile X mental retardation protein, members of the ELAV/Hu protein family, and the Staufen proteins are involved in targeting and translational regulation of dendritic transcripts [19–21]. Additionally, the finding that long-term memory requires de novo protein synthesis highlights the significance of post-transcriptional processes in neural function [22, 23].
Despite our knowledge of several key RBPs, much of the understanding of RBPs in the brain comes from studies of adult animals or neural cell lines. Thus, how the functional class of RBPs contributes to the positioning, growth, and diversification of cells in the developing brain is not well understood. One step towards increasing our understanding RBPs is to resolve where they are expressed. Here, we utilize the approach of in situ hybridization mapping [24–26] to investigate the expression of 323 RBPs within the developing mouse brain. Two stages of development were characterized, embryonic day 13.5 (E13.5), when critical cellular fate choice decisions are made and postnatal day 0 (P0), when the major structural components of the brain are apparent. We find that, in contrast to their proposed ubiquitous involvement in gene regulation, most RBPs are not uniformly expressed. The majority of RBPs profiled demonstrates spatially restricted expression in the brain or in other peripheral tissues examined. The data presented here and in an online database afford a visual filter for the functional analysis of individual RBPs in the developing mammalian NS.
Mouse RBPs were identified according to gene sequence
The RNA recognition motif (RRM), the hnRNP K-homology (KH) domain, and the double-stranded RNA-binding domain (dsRM) are evolutionarily conserved, well-characterized domains known to bind either single or double-stranded RNA [27–29]. Sequence similarity searches and structural analyses of these domains have led to the ability to predict other RBPs based on primary coding sequence . To identify unique genomic loci that encode putative RBPs in the mouse genome, we analyzed existing public [30, 31] and private  databases for sequences containing one or more RBD. Candidates were classified as RBPs only if their predicted protein sequence contained a Protein Families Database (Pfam)-defined RBD .
We identified 290 genes harboring one or more RRM, KH, or dsRM sequences. We also identified 32 genes encoding other domains shown to interact with RNA, including the zinc knuckle, G-patch, PIWI, DEAD box RNA helicase, and TUDOR domains. Finally, as the absence of a canonical RBD does not preclude interaction with RNA, we sought 58 additional genes known or predicted to be associated with RNA processing. In total, this collection contains 380 putative RBPs. Additional file 1 lists the number of genes, per RBD, identified and analyzed by in situ hybridization. A list of all genes and primer sequences is given in Additional file 2.
RBP expression in the developing mouse brain was analyzed by in situhybridization
To localize RBP expression, we preformed in situ hybridization on whole head tissue sections of E13.5 embryos and P0 mice. We designed gene-specific primers to produce 400–700 bp probes for 340 candidate RBPs. These primer sets were used to perform PCR on cDNA prepared from embryonic or P0 mouse brains. A small number of probes were obtained from mouse intestine, liver, kidney, or testes cDNA. 323 genes (95%) showed positive PCR products (data not shown). Following subcloning, anti-sense digoxygenin-labeled riboprobes were prepared and hybridized against coronal head and transverse upper-body sections (to include the brain and spinal cord, respectively). Digital images of the entire in situ hybridization set have been deposited in the Mahoney RNA-Binding Protein Expression Database .
RBPs exhibit restricted expression in the developing mouse brain
Several neural-specific RBPs have been identified, yet how many others demonstrate this degree of specificity is unknown. Of the genes examined we found 16 RBPs (listed in Additional file 2) that exhibit NS-restricted expression in the tissues analyzed. Among this list are known examples of neuronal-specific RBPs including Nova-1 , the ELAV/Hu proteins B, C, and D , and Ataxin 2 binding protein 1 (A2bp1)  but additionally include putative RBPs for which expression has not been reported. With the exception of one gene that was only detected at E13.5, all (15/16) of these RBPs appear brain or NS-specific at both developmental stages in the tissues analyzed. Overall, these RBP encoding genes are not limited in expression to one brain region but are found in multiple brain or NS structures.
RBPs show spatially restricted expression in anatomically distinct brain regions
We find that greater than half of the RBPs profiled exhibit spatially restricted expression. Of the 323 genes examined, 221 demonstrate localized, enriched expression in one or more discrete brain regions in addition to detectable expression in non-NS tissues. We divided the E13.5 and P0 CNS into five and eight general areas for annotation, respectively: the E13.5 precortical area, the striatum (and other basal ganglia), the periventrical areas, hindbrain, and spinal cord, as well as the P0 cortex, striatum, hippocampus, thalamus, hypothalamus, midbrain, hindbrain, and spinal cord. The presence or absence of expression for each RBP was analyzed visually at each location and is annotated in Additional file 3. Very few of the 221 RBPs with spatially restricted expression patterns were expressed in only one brain region, however most (73%) showed restricted expression at both developmental stages (Additional file 3).
RBPs demonstrate cell-type specific expression in the P0 mouse retina
A systems-based view of RBP expression
RBPs show restricted expression in non-NS tissues
Neural cells utilize multiple forms of post-transcriptional gene regulation. While RBPs are believed to be potent modulators of post-transcriptional processes, little is known about how this functional class is expressed in the developing brain. As a first step towards increasing our knowledge of RBPs we chose to investigate the spatial and temporal expression of genes that encode motifs known to interact with RNA. We find a small set of RBPs that show neural-specific expression in the tissues analyzed. An even greater number of RBP genes however demonstrate spatially restricted expression in distinct regions of the developing brain.
Within the CNS, most of the RBPs examined show non-uniform, heightened expression in anatomically discrete structures. Tissue differences in the expression levels of individual genes could indicate distinctive protein requirements among cell types, beyond that of tissue-specific RBPs . There is precedent for differential requirements of individual RBPs, as tissue-specific RNA splicing is achieved partly through combinatorial, stoichiometric differences among splicing factors within various cells . It is from this local enrichment within different cell types or tissues that we can begin to hypothesize as to the functional significance of individual genes as well as to the importance of groups of similarly expressed RBPs.
Our study has identified RBPs that display spatially restricted expression in distinct regions of the developing mouse brain. One set of RBPs (Fig. 1) is found in the E13.5 ventricular areas. A second set demonstrates spatially restricted expression in post-mitotic regions of E13.5 brain (Fig. 2). Based on their pattern of expression, these RBPs may have roles in neural proliferation, cell fate choice and cell migration, or in neuronal function, respectively. We also identified novel RBPs that are expressed in tissues of mesodermal and endodermal origin (Fig. 5). The highly restricted expression of these genes may indicate an explicit role for these RBPs in their respective epithelia. Additionally, the cell-type specificity RBPs found in the P0 retina (Fig. 3) illustrates the diversity of RBP expression. The specialized expression of these RBPs may be indicative of a dedicated function in the specified tissues.
By visual inspection of in situ hybridization data, we find a subset of RBPs that are coordinately expressed in multiple tissue types. These genes display heightened expression in the periventricular areas of the E13.5 brain and spinal cord as well as marked expression in the external granule layer of the P0 cerebellum, the lateral subventricular zones, and in teeth, nasal epithelia, and thymus (Fig. 4, Additional file 5, ). While not excluded from post-mitotic tissues, these RBPs are predominately expressed in structures that are undergoing cell division.
Notably, the term 'synexpression group' has been used to describe collections of genes that function in a common process and share a similar complex spatial expression pattern in multiple tissues . Among the synexpression group identified here we find examples of RBPs that are known to interact either physically or genetically (Additional file 4). For example, PTBP1 binds the splicing factors PSF  and hnRNP L  while SF2/ASF and hnRNP A1 select for 5' exon or exclusion or inclusion, respectively . Our data provide visual support to a growing body of evidence that functionally-related transcripts are post-transcriptionally co-regulated .
Although the significance of certain splicing and mRNA export factor enrichment in proliferating regions is not known, data from multiple studies point to a role for RBPs in cell proliferation. During hippocampal development expression levels of RBPs were found to be high and then to dramatically decrease, as neurons transition from a proliferating to a post-mitotic state . A number of RBPs were also identified as highly expressed in a molecular characterization of gastric epithelial progenitor cells [49, 50]. Furthermore, protein levels of hnRNPs and snRNPs were found to be down-regulated upon stimulated growth inhibition of myeloid cells . Therefore, it is likely that a role for RBPs during cell proliferation and cell fate determination exists in multiple tissue types.
In summary, the data presented here provide new insight into how a distinct functional gene class is expressed in the developing NS. We find that RBPs demonstrate region-specific as well as cell-type specific expression. In addition, we find that specific, proliferating regions of the embryonic and postnatal NS and peripheral tissues are similar in the expression of certain RBPs. These data serve as a starting point for functional investigations into the roles of RBPs in neural development and physiology.
In silicoRBP identification
Putative RBP gene sequences were identified by homology-based whole genome screening using public and private databases: Celera Panther Families, Protein Families Database (Pfam), and Genbank [30–32]. Classification as an RBP was based on the presence of one or more RRM, KH, or dsRMs, as defined by Pfam databases . Databases were also mined for zinc-knuckle, G-patch, PIWI, DEAD-box helicase and Tudor domain-containing sequences and for known factors involved in mRNA splicing, editing, transport, and stability. Genes with multiple RNA-binding domains were assigned to a single subfamily. Unique gene identity was verified by LocusID numbers. As of March 1, 2004, a total of 357 unique genes were identified from these sources. An additional 26 RRM, KH, and dsRM proteins have been identified as of March 7, 2005.
PCR primer design
PCR primer pairs were designed for each identified RNA-binding protein locus. PCR primer sequences were designed with approximately 60% GC content, spanning 400–700 base pairs of primarily the gene's coding sequence. Additional primer pairs were designed for targets that did not initially yield PCR products.
Total RNA was obtained from E13.5, P0, or adult C57/BL6 mouse brains (Charles River Laboratories) by Trizol extraction (Invitrogen). Reverse transcription was performed using Superscript II reverse transcriptase and oligo-dT (Invitrogen). PCR was performed with cDNA templates using 40 cycles, 60–65°C annealing temperature, and Platinum Taq (Invitrogen) as polymerase. For a few genes, PCR was performed with cDNA templates prepared from adult brain, kidney, gut, liver, or testis tissues. Positive PCR products were cloned into TA cloning vectors (Invitrogen) and verified by restriction digest or DNA sequencing.
Gene fragments from verified plasmids were amplified by PCR using plasmid specific primers. Digoxigenin-labeled RNA probes were made, using PCR products as template and T7 or SP6 RNA polymerases (Roche). cRNA probes were ethanol precipitated and quantified by spectrophotometry.
E13.5 embryos were directly fixed overnight in 4% paraformaldehyde (0.1M PBS). P0 mice were transcardially perfused with 4% paraformaldehyde (0.1M PBS) and postfixed overnight at 4°C. After fixation, embryos and P0 mice were transferred to 20% sucrose overnight. The head, neck, and trunk were embedded separately in OCT (Tissue-Tek) on dry ice and stored at -80°C. Serial cryostat sections (14 μm) were cut and mounted on Superfrost Plus slides (Fisher). Ten and twenty adjacent sets of sections were prepared from E13.5 embryos and P0 mice, respectively, and were stored at -20°C until use.
Section in situhybridization
In situ hybridization was performed according to Gray et al. . Following pretreatment (Proteinase K), slides were pre-hybridized for 1h at 65°C in hybridization solution (50% formamide (Ambion), 5X SSC, 0.3 mg/ml yeast tRNA (Sigma), 100 μg/ml heparin (Sigma), 1X Denhardt's (Sigma), 0.1% tween, 5 mM EDTA). P0 and E13.5 brain sections were hybridized overnight with labeled RNA probe(0.8–1.2 μg/ml) at 65°C, washed in 2X SSC at 67°C, incubated with RNase A (1 μg/ml, 2X SSC) at 37°C, washed in 0.2X SSC at 65°C, blocked in PBS with 10% lamb sera, and incubated in alkaline phosphatase labeled anti-DIG antibody (Roche) (1:2000, 10% sera) overnight. Sections were washed and color was visualized using NBT and BCIP in alkaline phosphatase buffer (100 mM Tris pH 9.5, 50 mM MgCl2, 100 mM NaCl, 0.1% tween-20) containing 75 μg/ml NBT (BioRad), 600 μg/ml BCIP (Roche). Staining was stopped after visual inspection. Sections were washed, fixed in 4% paraformaldehyde, and coverslipped in glycerol .
Image acquisition and RBP expression database
Images were acquired and analyzed as described . Images were either scanned using a Nikon Coolscan 8000 slide scanner (4000 DPI) or digitally acquired using a Leica digital camera. Image levels have been modified in Photoshop (Adobe) for clarity. Full resolution scanned images were compressed using JPEG compression, quality 10, and have been deposited in the Mahoney RNA-Binding Protein Expression Database .
We are grateful to Drs. Qiufu Ma and John Alberta for critical review of this manuscript and for assistance in this work. We thank Eric Tsung, Zhaohui Cai, and Matthew McCormack for designing the website. This work has been supported by the Bernard A. and Wendy J. Goldhirsh Foundation for Brain Tumor Research and by the Charles A. Dana Foundation. AEM is supported by an institutional training grant from the National Cancer Institute (T32CA09361). EM is funded as a FNRS Researcher through the Belgian National Research Fund and by the D. Collen Research Foundation VZW and BAEF. CS received support from the American Cancer Society (PF-02-128-01-MBC).
- Ross SE, Greenberg ME, Stiles CD: Basic helix-loop-helix factors in cortical development. Neuron. 2003, 39: 13-25. 10.1016/S0896-6273(03)00365-9.View ArticlePubMedGoogle Scholar
- Wilson SW, Houart C: Early steps in the development of the forebrain. Dev Cell. 2004, 6: 167-181. 10.1016/S1534-5807(04)00027-9.PubMed CentralView ArticlePubMedGoogle Scholar
- Bally-Cuif L, Hammerschmidt M: Induction and patterning of neuronal development, and its connection to cell cycle control. Curr Opin Neurobiol. 2003, 13: 16-25. 10.1016/S0959-4388(03)00015-1.View ArticlePubMedGoogle Scholar
- Dreyfuss G, Kim VN, Kataoka N: Messenger-RNA-binding proteins and the messages they carry. Nat Rev Mol Cell Biol. 2002, 3: 195-205. 10.1038/nrm760.View ArticlePubMedGoogle Scholar
- Lasko P: Gene regulation at the RNA layer: RNA binding proteins in intercellular signaling networks. Sci STKE. 2003, 2003: RE6-PubMedGoogle Scholar
- Orphanides G, Reinberg D: A unified theory of gene expression. Cell. 2002, 108: 439-451. 10.1016/S0092-8674(02)00655-4.View ArticlePubMedGoogle Scholar
- Cullen BR: Transcription and processing of human microRNA precursors. Mol Cell. 2004, 16: 861-865. 10.1016/j.molcel.2004.12.002.View ArticlePubMedGoogle Scholar
- Huang YS, Carson JH, Barbarese E, Richter JD: Facilitation of dendritic mRNA transport by CPEB. Genes Dev. 2003, 17: 638-653. 10.1101/gad.1053003.PubMed CentralView ArticlePubMedGoogle Scholar
- Antar LN, Bassell GJ: Sunrise at the synapse: the FMRP mRNP shaping the synaptic interface. Neuron. 2003, 37: 555-558. 10.1016/S0896-6273(03)00090-4.View ArticlePubMedGoogle Scholar
- Tang SJ, Meulemans D, Vazquez L, Colaco N, Schuman E: A role for a rat homolog of staufen in the transport of RNA to neuronal dendrites. Neuron. 2001, 32: 463-475. 10.1016/S0896-6273(01)00493-7.View ArticlePubMedGoogle Scholar
- Martin KC: Local protein synthesis during axon guidance and synaptic plasticity. Curr Opin Neurobiol. 2004, 14: 305-310. 10.1016/j.conb.2004.05.009.View ArticlePubMedGoogle Scholar
- Huang YS, Richter JD: Regulation of local mRNA translation. Curr Opin Cell Biol. 2004, 16: 308-313. 10.1016/j.ceb.2004.03.002.View ArticlePubMedGoogle Scholar
- Agnes F, Perron M: RNA-binding proteins and neural development: a matter of targets and complexes. Neuroreport. 2004, 15: 2567-2570. 10.1097/00001756-200412030-00001.View ArticlePubMedGoogle Scholar
- Perrone-Bizzozero N, Bolognani F: Role of HuD and other RNA-binding proteins in neural development and plasticity. J Neurosci Res. 2002, 68: 121-126. 10.1002/jnr.10175.View ArticlePubMedGoogle Scholar
- Ule J, Jensen KB, Ruggiu M, Mele A, Ule A, Darnell RB: CLIP identifies Nova-regulated RNA networks in the brain. Science. 2003, 302: 1212-1215. 10.1126/science.1090095.View ArticlePubMedGoogle Scholar
- Jensen KB, Dredge BK, Stefani G, Zhong R, Buckanovich RJ, Okano HJ, Yang YY, Darnell RB: Nova-1 regulates neuron-specific alternative splicing and is essential for neuronal viability. Neuron. 2000, 25: 359-371. 10.1016/S0896-6273(00)80900-9.View ArticlePubMedGoogle Scholar
- Larocque D, Galarneau A, Liu HN, Scott M, Almazan G, Richard S: Protection of p27(Kip1) mRNA by quaking RNA binding proteins promotes oligodendrocyte differentiation. Nat Neurosci. 2005, 8: 27-33. 10.1038/nn1359.View ArticlePubMedGoogle Scholar
- Sakakibara S, Nakamura Y, Yoshida T, Shibata S, Koike M, Takano H, Ueda S, Uchiyama Y, Noda T, Okano H: RNA-binding protein Musashi family: roles for CNS stem cells and a subpopulation of ependymal cells revealed by targeted disruption and antisense ablation. Proc Natl Acad Sci U S A. 2002, 99: 15194-15199. 10.1073/pnas.232087499.PubMed CentralView ArticlePubMedGoogle Scholar
- Pascale A, Gusev PA, Amadio M, Dottorini T, Govoni S, Alkon DL, Quattrone A: Increase of the RNA-binding protein HuD and posttranscriptional up-regulation of the GAP-43 gene during spatial memory. Proc Natl Acad Sci U S A. 2004, 101: 1217-1222. 10.1073/pnas.0307674100.PubMed CentralView ArticlePubMedGoogle Scholar
- Jin P, Alisch RS, Warren ST: RNA and microRNAs in fragile X mental retardation. Nat Cell Biol. 2004, 6: 1048-1053. 10.1038/ncb1104-1048.View ArticlePubMedGoogle Scholar
- Dubnau J, Chiang AS, Grady L, Barditch J, Gossweiler S, McNeil J, Smith P, Buldoc F, Scott R, Certa U, Broger C, Tully T: The staufen/pumilio pathway is involved in Drosophila long-term memory. Curr Biol. 2003, 13: 286-296. 10.1016/S0960-9822(03)00064-2.View ArticlePubMedGoogle Scholar
- Miller S, Yasuda M, Coats JK, Jones Y, Martone ME, Mayford M: Disruption of dendritic translation of CaMKIIalpha impairs stabilization of synaptic plasticity and memory consolidation. Neuron. 2002, 36: 507-519. 10.1016/S0896-6273(02)00978-9.View ArticlePubMedGoogle Scholar
- Kang H, Schuman EM: A requirement for local protein synthesis in neurotrophin-induced hippocampal synaptic plasticity. Science. 1996, 273: 1402-1406.View ArticlePubMedGoogle Scholar
- Reymond A, Marigo V, Yaylaoglu MB, Leoni A, Ucla C, Scamuffa N, Caccioppoli C, Dermitzakis ET, Lyle R, Banfi S, Eichele G, Antonarakis SE, Ballabio A: Human chromosome 21 gene expression atlas in the mouse. Nature. 2002, 420: 582-586. 10.1038/nature01178.View ArticlePubMedGoogle Scholar
- Gray PA, Fu H, Luo P, Zhao Q, Yu J, Ferrari A, Tenzen T, Yuk DI, Tsung EF, Cai Z, Alberta JA, Cheng LP, Liu Y, Stenman JM, Valerius MT, Billings N, Kim HA, Greenberg ME, McMahon AP, Rowitch DH, Stiles CD, Ma Q: Mouse brain organization revealed through direct genome-scale TF expression analysis. Science. 2004, 306: 2255-2257. 10.1126/science.1104935.View ArticlePubMedGoogle Scholar
- Gitton Y, Dahmane N, Baik S, Ruiz i Altaba A, Neidhardt L, Scholze M, Herrmann BG, Kahlem P, Benkahla A, Schrinner S, Yildirimman R, Herwig R, Lehrach H, Yaspo ML: A gene expression map of human chromosome 21 orthologues in the mouse. Nature. 2002, 420: 586-590. 10.1038/nature01270.View ArticlePubMedGoogle Scholar
- Saunders LR, Barber GN: The dsRNA binding protein family: critical roles, diverse cellular functions. Faseb J. 2003, 17: 961-983. 10.1096/fj.02-0958rev.View ArticlePubMedGoogle Scholar
- Nagai K: RNA-protein complexes. Curr Opin Struct Biol. 1996, 6: 53-61. 10.1016/S0959-440X(96)80095-9.View ArticlePubMedGoogle Scholar
- Burd CG, Dreyfuss G: Conserved structures and diversity of functions of RNA-binding proteins. Science. 1994, 265: 615-621.View ArticlePubMedGoogle Scholar
- Wheeler DL, Church DM, Edgar R, Federhen S, Helmberg W, Madden TL, Pontius JU, Schuler GD, Schriml LM, Sequeira E, Suzek TO, Tatusova TA, Wagner L: Database resources of the National Center for Biotechnology Information: update. Nucleic Acids Res. 2004, 32: D35-40. 10.1093/nar/gkh073.PubMed CentralView ArticlePubMedGoogle Scholar
- Sonnhammer EL, Eddy SR, Birney E, Bateman A, Durbin R: Pfam: multiple sequence alignments and HMM-profiles of protein domains. Nucleic Acids Res. 1998, 26: 320-322. 10.1093/nar/26.1.320.PubMed CentralView ArticlePubMedGoogle Scholar
- Thomas PD, Campbell MJ, Kejariwal A, Mi H, Karlak B, Daverman R, Diemer K, Muruganujan A, Narechania A: PANTHER: a library of protein families and subfamilies indexed by function. Genome Res. 2003, 13: 2129-2141. 10.1101/gr.772403.PubMed CentralView ArticlePubMedGoogle Scholar
- http://mahoney.chip.org/mahoney/RBP.Google Scholar
- Buckanovich RJ, Posner JB, Darnell RB: Nova, the paraneoplastic Ri antigen, is homologous to an RNA-binding protein and is specifically expressed in the developing motor system. Neuron. 1993, 11: 657-672. 10.1016/0896-6273(93)90077-5.View ArticlePubMedGoogle Scholar
- Okano HJ, Darnell RB: A hierarchy of Hu RNA binding proteins in developing and adult neurons. J Neurosci. 1997, 17: 3024-3037.PubMedGoogle Scholar
- Kiehl TR, Shibata H, Vo T, Huynh DP, Pulst SM: Identification and expression of a mouse ortholog of A2BP1. Mamm Genome. 2001, 12: 595-601. 10.1007/s00335-001-2056-4.View ArticlePubMedGoogle Scholar
- Sakakibara S, Okano H: Expression of neural RNA-binding proteins in the postnatal CNS: implications of their roles in neuronal and glial cell development. J Neurosci. 1997, 17: 8300-8312.PubMedGoogle Scholar
- Sakakibara S, Nakamura Y, Satoh H, Okano H: Rna-binding protein Musashi2: developmentally regulated expression in neural precursor cells and subpopulations of neurons in mammalian CNS. J Neurosci. 2001, 21: 8091-8107.PubMedGoogle Scholar
- Blackshaw S, Harpavat S, Trimarchi J, Cai L, Huang H, Kuo WP, Weber G, Lee K, Fraioli RE, Cho SH, Yung R, Asch E, Ohno-Machado L, Wong WH, Cepko CL: Genomic analysis of mouse retinal development. PLoS Biol. 2004, 2: E247-10.1371/journal.pbio.0020247.PubMed CentralView ArticlePubMedGoogle Scholar
- Lillevali K, Kulla A, Ord T: Comparative expression analysis of the genes encoding polypyrimidine tract binding protein (PTB) and its neural homologue (brPTB) in prenatal and postnatal mouse brain. Mech Dev. 2001, 101: 217-220. 10.1016/S0925-4773(00)00566-9.View ArticlePubMedGoogle Scholar
- Zhang W, Liu H, Han K, Grabowski PJ: Region-specific alternative splicing in the nervous system: implications for regulation by the RNA-binding protein NAPOR. Rna. 2002, 8: 671-685. 10.1017/S1355838202027036.PubMed CentralView ArticlePubMedGoogle Scholar
- Grabowski PJ, Black DL: Alternative RNA splicing in the nervous system. Prog Neurobiol. 2001, 65: 289-308. 10.1016/S0301-0082(01)00007-7.View ArticlePubMedGoogle Scholar
- Niehrs C, Pollet N: Synexpression groups in eukaryotes. Nature. 1999, 402: 483-487. 10.1038/990025.View ArticlePubMedGoogle Scholar
- Patton JG, Porro EB, Galceran J, Tempst P, Nadal-Ginard B: Cloning and characterization of PSF, a novel pre-mRNA splicing factor. Genes Dev. 1993, 7: 393-406.View ArticlePubMedGoogle Scholar
- Hahm B, Cho OH, Kim JE, Kim YK, Kim JH, Oh YL, Jang SK: Polypyrimidine tract-binding protein interacts with HnRNP L. FEBS Lett. 1998, 425: 401-406. 10.1016/S0014-5793(98)00269-5.View ArticlePubMedGoogle Scholar
- Eperon IC, Makarova OV, Mayeda A, Munroe SH, Caceres JF, Hayward DG, Krainer AR: Selection of alternative 5' splice sites: role of U1 snRNP and models for the antagonistic effects of SF2/ASF and hnRNP A1. Mol Cell Biol. 2000, 20: 8303-8318. 10.1128/MCB.20.22.8303-8318.2000.PubMed CentralView ArticlePubMedGoogle Scholar
- Hieronymus H, Silver PA: A systems view of mRNP biology. Genes Dev. 2004, 18: 2845-2860. 10.1101/gad.1256904.View ArticlePubMedGoogle Scholar
- Mody M, Cao Y, Cui Z, Tay KY, Shyong A, Shimizu E, Pham K, Schultz P, Welsh D, Tsien JZ: Genome-wide gene expression profiles of the developing mouse hippocampus. Proc Natl Acad Sci U S A. 2001, 98: 8862-8867. 10.1073/pnas.141244998.PubMed CentralView ArticlePubMedGoogle Scholar
- Mills JC, Andersson N, Hong CV, Stappenbeck TS, Gordon JI: Molecular characterization of mouse gastric epithelial progenitor cells. Proc Natl Acad Sci U S A. 2002, 99: 14819-14824. 10.1073/pnas.192574799.PubMed CentralView ArticlePubMedGoogle Scholar
- Stappenbeck TS, Hooper LV, Gordon JI: Developmental regulation of intestinal angiogenesis by indigenous microbes via Paneth cells. Proc Natl Acad Sci U S A. 2002, 99: 15451-15455. 10.1073/pnas.202604299.PubMed CentralView ArticlePubMedGoogle Scholar
- Harris MN, Ozpolat B, Abdi F, Gu S, Legler A, Mawuenyega KG, Tirado-Gomez M, Lopez-Berestein G, Chen X: Comparative proteomic analysis of all-trans-retinoic acid treatment reveals systematic posttranscriptional control mechanisms in acute promyelocytic leukemia. Blood. 2004, 104: 1314-1323. 10.1182/blood-2004-01-0046.View ArticlePubMedGoogle Scholar
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