- Research article
- Open Access
The frizzledpathway regulates the development of arista laterals.
© He and Adler; licensee BioMed Central Ltd. 2002
Received: 25 March 2002
Accepted: 3 May 2002
Published: 3 May 2002
The frizzled pathway in Drosophila has been studied intensively for its role in the development of planar polarity in wing hairs, thoracic bristles and ommatidia. Selected cells in the arista (the terminal segment of the antenna) elaborate a lateral projection that shares characteristics with both hairs and bristles.
We found that mutations in putative downstream members of the frizzled pathway, such as inturned resulted in multipled and split laterals, but no obvious polarity defects. Mutations in upstream genes such as frizzled did not show an adult arista phenotype. When we examined lateral development in the mutants we found that, as is the case on the wing for hair development the frizzled pathway regulated the subcellular location for lateral initiation. However, on the arista an altered location for lateral initiation did not result in altered polarity, as did an altered site for hair initiation. The regulation of lateral development involved the preferential accumulation of Frizzled protein at the distal edges of lateral cells much as is seen on the wing. In contrast to the situation in wing cells, in arista cells the location for lateral initiation was close to but did not overlap the region of preferential Frizzled accumulation.
Our data indicates that a modified version of the frizzled pathway regulates arista development. We conclude that the lack of a polarity defect in mutant aristae is likely to be a consequence of inherent differences in the cell biology of wing hair and lateral forming cells.
The adult cuticular surface of insects is covered with hairs, sensory bristles, ridges, scales and other structures. In most body regions individual cuticular structures are aligned in the plane of the epithelium. The basis for the development of this planar or tissue polarity has principally been studied in Drosophila [1–4] where it is under the control of the frizzled (fz) pathway . Many Drosophila epidermal cells produce cuticular hairs that are outgrowths from the apical surface of pupal epidermal cells. The development of epidermal hairs has principally been studied on the wing where each pupal cell produces a single distally pointing hair. The fz pathway controls hair polarity by directing the location for hair initiation . In a wild type fly hairs are formed at the distal most vertex of the wing cells and extend distally. Mutations in fz pathway genes result in altered sites for hair initiation, which results in altered hair polarity and in some cases altered hair number . Sensory bristles are found in all regions of the adult epidermis. Bristles are polarized within the plane of the epidermis and in most body region neighbouring bristles are aligned in parallel and point in the same direction as neighbouring hairs. The fz pathway controls bristle polarity by regulating the orientation of the spindle for the first differentiative division of the sensory organ precursor cell . The ommatidia of the compound eye also display a dramatic planar polarity that is under the control of the fz pathway. In this tissue the fz pathway controls polarity by regulating the R3/R4 cell fate decision .
The arista is the terminal segment of the antenna and it is comprised of a central core of epidermal cells, some of which elaborate a single lateral extension . The laterals and the cells that form them share a number of characteristics of both hairs and bristles. The laterals are longer than many bristles, but thinner. At the ultrastructural level developing laterals resemble bristles and not hairs . They contain a series of large bundles of F-actin that are juxtaposed to the plasma membrane. In addition, microtubules are distributed throughout the central region of the lateral. The developing laterals resemble hairs and not bristle in that they are formed at the apical surface of epithelial cells and they are not innervated.
Mutations in the genes of the fz pathway result in altered hair and bristle polarity and in some cases large numbers of cells that produce multiple hairs. To determine if the fz pathway played a role in arista development we examined the aristae of various fz pathway mutants [1, 5]. We found that multiple wing hairs (mwh), which results in an average of almost 4 hairs per cell on the wing also resulted in the formation of multipled and branched laterals. However unlike the situation on the wing, where mwh results in altered hair polarity, on the arista we did not see any evidence of altered lateral polarity. We also examined aristae from inturned (in) and fuzzy (fy) mutants. On the wing mutations in these genes result in altered hair polarity and in many cells forming two or three hairs . On mutant aristae we found that in and fy caused multipled and branched laterals, but as was the case for mwh we did not see any evidence for altered polarity. We did not detect any mutant phenotype in adult aristae of fz, Van Gogh (Vang), dishevelled (dsh), starry night (stan) also known as - Flamingo (fmi) or prickle (pk) mutants . It was surprising that upstream members of the fz pathway did not show a mutant phenotype while downstream members did. We therefore examined the situation further. In developing wild type pupal aristae laterals formed near but not at the distal edge of aristae central core cells . When we examined fz, dsh andstan mutants we noted that lateral initiation was no longer restricted to the distal region of central core cells. Although these laterals formed at an unusual subcellular location this did not produce a noticeable phenotype in the adult. The altered location for lateral initiation in fz and other mutants was similar to their phenotype in the wing . Prior to hair initiation in wing cells Fz, Dsh, Fmi and Dgo accumulate on the distal side of wing cells (Stan and Dgo are also thought to accumulate on the proximal side) [9–13]. To see if this could be the case for lateral development we examined the distribution of Fz in developing arista using a Fz-GFP encoding transgene gene. We found that Fz-GFP preferentially accumulated on the distal side of arista central core cells much as it does in wing cells. In addition, we found that this asymmetric localization was lost in dsh mutants but retained in in and fy mutants as is the case in wing cells. As is the case in wing cells the distal accumulation of Fz did not appear to influence lateral development in an in or fy mutant. Overall the data argue that the fz pathway functions in a similar but modified way to regulate lateral morphogenesis as compared to wing hair morphogenesis. We suggest that the absence of a noticeable adult phenotype in fz aristae while there is a dramatic phenotype on the wing is due to inherent differences in the cell biology of the relevant cells.
The arista phenotype of inturned and fuzzy
The adult arista phenotypes in single and selected double mutants.
Number of lateral ends per cell mean (sd)
P value compared to (t-test)
p < 0.0001 (Ore R)
p < 0.0001 (Ore R)
p = 0.94 (fy), 0.80 (in)
p = 0.67 (in)
p = 0.90 (fy)
p = 0.29 (fy)
p < 0.0001 (Ore R)
p = 0.68 (mwh)
p = 0.002 (in), 0.0005 (mwh)
p = 0.0001 (fy), 0.007 (mwh)
frizzled pathway mutations and the position of lateral formation
P* compared to Oregon R
P compared to frizzled
2 × 10-10
2 × 10-10
6.2 × 10-14
1.3 × 10-4
3.5 × 10-2
1 × 10-6
1 × 10-2
3.5 × 10-6
3.9 × 10-5
4.2 × 10-7
On the wing single and double mutant combinations of in and fy result in a similar phenotype . This lack of additivity has been interpreted as evidence that these genes function and are required in a common process. We compared the multipled lateral phenotypes of in and fy single mutants and fy; in double mutants and found no difference consistent with in and fy functioning in the same process in lateral development (Table 1). In the wing in and fy are epistatic to mutations in fz, dishevelled (dsh), prickle (pk), Van Gogh (Vang) (aka strabismus) and starry night (stan) (aka flamingo) [5, 21]. This was also the case in the arista (Table 1).
The arista phenotype of mutiple wing hairs
In the wing multiple wing hairs (mwh) results in similar polarity disruptions and a stronger multiple hair cell phenotype than in or fy[5, 14]. mwh aristae had almost 5 lateral ends per cell (Table 1) (Fig. 1D). The laterals were also substantially shorter than wild type (40–45% of normal length). Thus, in the arista laterals as in wing hairs mwh has a substantially stronger phenotype than in or fy. At early stages in mwh lateral morphogenesis we saw multiple locations within individual cells where F-actin accumulated (Fig. 1H). At later stages these appeared as multiple independent laterals produced by individual cells (Figure 1L,1M). These conclusions were confirmed by time-lapse observations ((data not shown)). In addition, we saw examples where a lateral split during outgrowth ((data not shown)). There was no alteration in the time of lateral initiation in mwh although we did see a decrease in the rate of lateral elongation that was consistent with the short final length of mwh laterals ((data not shown)). The short length and slow growth may simply be a consequence of the extreme multiple lateral phenotype as laterals that were only slightly branched were of relatively normal length. In the wing all double mutant combinations of mwh and other fz pathway genes result in a mwh like phenotype . For the lateral phenotype we found that mwh fz double mutants resembled mwh, as is the case in the wing (Table 1). However, mwh in and fy; mwh laterals produced an intermediate phenotype in terms of the mean number of lateral ends per cell (Table 1). Thus, the relationship between in and fy and mwh appears different in the wing and arista, presumably reflecting differences in the genetic circuitry in these two tissues.
The arista phenotype of fz mutants
We did not see any obvious mutant phenotype in aristae from fz, dsh, pk, Vang or stan flies (Fig. 1B). In the wing, mutations in fz and these other fz like genes result in hairs with abnormal polarity due to a failure to restrict prehair initiation to the distal most part of the cell . Only infrequently does this result in a cell forming more than one hair. When we examined the development of fz mutant laterals by phalloidin staining we found that lateral initiation was not as restricted to the distal part of cells as is the case in wild type (Fig. 1F) (Table 2). Rather laterals formed at a wide range of locations along the proximal distal axis of the extended central core cells. For fz, dsh (Fig. 1G) and stan (data not shown) the lateral formed at about 70% of the way toward the distal edge of the cell, which was significantly different from that of wild type. This was slightly more distal than we saw for in and fy but not significantly so (Table 2). For pk and Vang mutants the average location for lateral initation was slightly less distal than in wild type (≅ 85% vs 90%) and this difference was only marginally significant. The position for lateral initiation in pk and Vang was clearly different from that of fz.
Fz accumulates asymmetrically in arista cells
In the wing the accumulation of Fz at the distal edge of the cell requires the function of dsh. We examined aristae from dsh1; arm-fz-GFP pupae and found that the preferential distal accumulation of Fz was lost (Fig. 4). The ratio of GFP fluorescence on the distal versus laterals sides of cells ranged from 1.2 – 0.9. Thus, as in the wing the distal accumulation of Fz in arista cells is dependent on dsh function. In the wing Dsh accumulates preferentially along the distal edge of wing cells [12, 13]. We found that Dsh-GFP also accumulated along the distal edge of arista cells (Fig. 4).
In the wing the distal accumulation of Fz is not dependent on the function of in or fy, although in in and fy mutants the distal accumulation of Fz is no longer able to restrict hair formation to the distal edge of the cell . We examined the distribution of Fz in arm-fz-GFP; in and fy; arm-fz-GFP aristae. The preferential distal accumulation of Fz was still present in these arista cells (Fig. 4) (we obtained distal to lateral edge ratios of 1.7 – 2.0), although the in and fy mutant lateral phenotype was obvious. Thus, fz, in and fy appear to have a similar relationship in the arista and wing.
Based on the precedent from wing cells it is at first glance surprising that an alteration in the site for lateral initiation does not produce obvious phenotypic consequences . Pupal wing cells are relatively isotropic in terms of their shape in the planar direction. The assembly of the hair at the cell periphery puts it in close proximity to the junctional complex perhaps allowing for direct interactions between the cortical and hair cytoskeletons . This could provide orientation guidance for the growing hair. In a fz mutant, where hair formation no longer takes place at the cell periphery this guidance would be lost (Fig. 7). In an in mutant, where hair formation takes place at alternative locations on the cell periphery, it would be incorrect (Fig. 7). Lateral forming cells are elongated in the proximal distal direction (Fig. 7) and laterals grow out at a large angle to the apical surface of the central core cells. In this way wild type laterals resemble fz mutant wing cells . Thus, early in their development laterals are not close to the junctional complex or the prominent cortical cytoskeleton at the cell periphery and are unlikely to be getting any orientation guidance in this way. In a fz mutant lateral the location along the proximal distal axis of the cell is changed but there is no disruption of a close connection to the cortical cytoskeleton as there is in hair cells. In the mutant there is no change in the dorsal/ventral position for lateral outgrowth or the angle of the outgrowth to the apical surface. In part this may be due to limitations inherent in a relatively large lateral growing out of a long thin cell. It is also worth considering that the elongated shape of the lateral forming cells becomes much more extreme as development proceeds (Fig 4) . This brings the laterals close to the cell edges that are parallel to the proximal distal axis of the central core and the junctional complexes at these edges could provide orientation guidance.
Previous studies on in and fy function in regulating wing hair development have focused on their role in regulating the site for hair initiation [5, 20]. Observations reported in this paper show that multiple laterals associated with in and fy mutants can arise either from independent initiation events or from the splitting of an elongating lateral. The splitting of laterals could be due to a need for in and fy function during the outgrowth process or to a need for in and fy function at initiation with the consequences only being manifested at a later time. We carried out a set of temperature shift experiments to distinguish between these possibilities. These shift experiments showed that the essential time for in function in lateral development is around the time of lateral initiation. This is similar to what we previously found for in function in wing hair development . It is interesting that the consequences of a lack of in function in laterals are manifested some hours after the end of the in temperature sensitive period. We suggest that in an in mutant there is a subtle defect in a cytoskeleton-organizing center that promotes lateral outgrowth. The defect is not severe enough to block or dramatically slow lateral outgrowth. In some cells it leads to the formation of multiple initiation sites and in others to an unstable lateral that is subject to splitting. A similar phenomenon could explain the formation of occasional branched wing hairs in in, fy and mwh wings. It could also explain differences in the hair, lateral and bristle phenotypes of tricornered (trc) and furry (fry) mutants [19, 26]. In such mutants bristles and laterals are often branched. In contrast, trc and fry wing cells have a very dramatic multiple hair cells phenotype (average more than 5 hairs per cell). The multiple hairs are found primarily in clusters of independent hairs with only occasional branched hairs. We suggest that there is a common defect in trc and fry bristles, laterals and hairs. Differences in the phenotypes would be due to inherent differences in the sensitivity of these cell types to the defective organizing centers. The smaller hairs would be more sensitive to the defects leading to "spitting" during the initial stages of growth, which would result in what appears to be a cluster of independent hairs. The larger structures (laterals and bristles) could be less sensitive so that the consequences of the abnormal organizing center would only be manifested later in morphogenesis if at all.
Previous observations had indicated that the fz pathway functioned in the development of planar polarity in hairs, bristles and ommatidia [5–7, 14]. Consistent with much current thinking on signal transduction pathways it has been suggested that there is a core group of fz pathway of genes that function similarly in different cell types . Downstream genes would function to interpret the signal from the core group of genes in a cell type specific way. As attractive as this idea is a number of observations suggest that much of the fz pathway is modified in cell type specific ways. For example, the asymmetric accumulation of Fmi/Stan in wing cells is essential for marking the distal edge of wing cells . However, Fmi/Stan does not accumulate asymmetrically in sensory organ precursor cell that gives rise to the bristle sense organ although the orientation of the pI cell spindle is altered in fmi/stan mutants . Our observations on the arista suggest that the genetic circuitry of the fz pathway is modified in a different way in this cell type. The most notable differences were the lack of a substantial phenotype in pk and Vang and that mwh is not epistatic to in and fy as it the case on the wing.
In this paper we provide evidence that a modified version of the frizzled pathway regulates the development of arista laterals. As is the case for the wing the asymmetrical accumulation of Fz protein appears to produce a cortical mark that is used to control where the cytoskeleton is activated to produce a lateral.
Materials and methods
Fruit flies were grown on standard media. Many of the mutations were isolated in this lab and described previously [5, 20, 27, 28]. Several were obtained from the Drosophila stock center at Indiana University. D. Strutt kindly provided flies carrying the arm-fz-GFP transgene  and J. Axelrod kindly provided flies carrying a dsh-GFP transgene (this construct uses the endogenous dsh promoter) . Temperature shift experiments were done as previously .
Standard techniques were used. In most staining experiments pupae were fixed in phosphate buffered saline supplemented with 4% paraformaldehyde. Actin staining was done as described previously  using Alexa 568 phalloidin (Molecular Probes). For experiments where we visualized GFP we made an effort to use the minimum amount of fixation compatible with maintaining the morphology of the aristae.
Confocal images were obtained using a BioRad confocal microscope at the Keck Center for Biological Imaging at the University of Virginia. Other images were obtained using a Spot digital camera (National Diagnostics) on a Zeiss Axioskop microscope using bright field optics. Length measurements were made using either the software provided with the Spot camera or by using the ruler tool in Adobe Photoshop. To measure the intensity of GFP-fluorescence we took maximal projections of optical sections and used the Psion port of the NIH Image software to analyze the data. The tif images were inverted and the intensity plotted along an 8 pixel line drawn across the relevant cell boundary. The background was estimated by the lowest reading along the line and this was subtracted from the highest reading to obtain an intensity measurement. The same procedure was done for a line drawn along a "low" intensity cell border. Multiple cells were scored (usually 10) from the same projection, averages obtained used to provide an intensity ratio. Microsoft Excel was used to analyze the data (calculating means, standard deviation, and plotting). To measure the location along the proximal/distal axes of arista cells we took micrographs of actin stained aristae where the lateral was small (less than 1/3 the length of the cell) and measured the distance from the proximal to distal sides of the cell. We also measured the distance from the proximal side to the distal edge of the base of the growing lateral where it was connected to the central core. The ratio of these two values is used as a measure of the location for lateral initiation.
Scoring of arista phenotypes
Adult aristae were mounted in Gary's Magic Mountant and examined under bright field microscope.
In vivoimaging of the arista laterals
Carefully aged pupae were washed and air-dried. In early experiments they were then attached to standard microscope slides with double-stick tape or to shallow well slides using super glue. In both preparations the animals were placed with their ventral side up. Pupal cases were opened anteriorly. At various time points they were examined by microscope under bright field optics in a drop of water using a water immersion objective (40×). After observation water was removed from the slides and the pupae were then kept in a humid chamber at 25°C for further development and observations. Most pupae tolerated the treatment, reached the pharate adult stage and were morphologically normal. More recently we built a chamber using rubber spacers, double stick tape and a cover-slip. The pupae were placed on the double stick tape ventral side up and the pupal case opened. A small amount of 10% gelatin, 10% glycerol was place on the anterior end of the pupae and between the opened pupae and the cover slip. Although the optics are not quite as good as with our earlier approaches, the chamber approach is less time consuming and can be used for automated time lapse.
Excel was used in the quantitative analysis of phenotypes. We used t-test and Z test in different experiments for comparing different genotypes.
This research was supported by a grant from the NIGMS to PNA.
We thank David Strutt for kindly providing the arm fz-GFP transgenic flies and Jeff Axelrod for the dsh-GFP flies. We thank Job Taylor for his helpful comments on the manuscript.
- Adler P, Lee H: Frizzled signaling and cell-cell interactions in planar polarity. Curr Opin Cell Biol. 2001, 13: 635-640. 10.1016/S0955-0674(00)00263-5.View ArticlePubMedGoogle Scholar
- Shulman JM, Perrimon N, Axelrod JD: Frizzled signaling and the developmental control of cell polarity. Trends Genet. 1998, 14: 452-8. 10.1016/S0168-9525(98)01584-4.View ArticlePubMedGoogle Scholar
- Strutt H, Strutt D: Polarity determination in the Drosophila eye. Curr Opin Genet Dev. 1999, 9: 442-6. 10.1016/S0959-437X(99)80067-7.View ArticlePubMedGoogle Scholar
- Adler PN, Taylor J: Asymmetric cell division: Plane but not simple. Curr Biol. 2001, 11: R233-6. 10.1016/S0960-9822(01)00113-0.View ArticlePubMedGoogle Scholar
- Wong LL, Adler PN: Tissue polarity genes of Drosophila regulate the subcellular location for prehair initiation in pupal wing cells. J Cell Biol. 1993, 123: 209-21.View ArticlePubMedGoogle Scholar
- Gho M, Schweisguth F: Frizzled signalling controls orientation of asymmetric sense organ precursor cell divisions in Drosophila. Nature. 1998, 393: 178-81. 10.1038/30265.View ArticlePubMedGoogle Scholar
- Zheng L, Zhang J, Carthew RW: frizzled regulates mirror-symmetric pattern formation in the Drosophila eye. Development. 1995, 121: 3045-55.PubMedGoogle Scholar
- He B, Adler PN: Cellular mechanisms in the development of the epithelial arista. Mechanisms of Development. 2001Google Scholar
- Usui T, Shima Y, Shimada Y, Hirano S, Burgess RW, Schwarz TL, Takeichi M, Uemura T: Flamingo, a seven-pass transmembrane cadherin, regulates planar cell polarity under the control of Frizzled. Cell. 1999, 98: 585-95.View ArticlePubMedGoogle Scholar
- Strutt DI: Asymmetric localization of frizzled and the establishment of cell polarity in the Drosophila wing. Mol Cell. 2001, 7: 367-75.View ArticlePubMedGoogle Scholar
- Feiguin F, Hannus M, Mlodzik M, Eaton S: The ankyrin repeat protein Diego mediates Frizzled-dependent planar polarization. Dev Cell. 2001, 1: 93-101.View ArticlePubMedGoogle Scholar
- Axelrod JD: Unipolar membrane association of Dishevelled mediates Frizzled planar cell polarity signaling. Genes Dev. 2001, 15: 1182-7.PubMed CentralPubMedGoogle Scholar
- Shimada Y, Usui T, Yanagawa S, Takeichi M, Uemura T: Asymmetric colocalization of Flamingo, a seven-pass transmembrane cadherin, and Dishevelled in planar cell polarization. Curr Biol. 2001, 11: 859-63. 10.1016/S0960-9822(01)00233-0.View ArticlePubMedGoogle Scholar
- Gubb D, Garcia-Bellido A: A genetic analysis of the determination of cuticular polarity during development in Drosophila melanogaster. J Embryol Exp Morphol. 1982, 68: 37-57.PubMedGoogle Scholar
- Collier S, Gubb D: Drosophila tissue polarity requires the cell-autonomous activity of the fuzzy gene, which encodes a novel transmembrane protein. Development. 1997, 124: 4029-4037.PubMedGoogle Scholar
- Park WJ, Liu J, Sharp EJ, Adler PN: The Drosophila tissue polarity gene inturned acts cell autonomously and encodes a novel protein. Development. 1996, 122: 961-9.PubMedGoogle Scholar
- He B: Genetic Control of Cell Extensions. Charlottesville: University of Virginia;. 2001Google Scholar
- He B, Adler PN: Genetic control of lateral morphogenesis. Development, genes and evolution. 2002Google Scholar
- Cong J, Geng W, He B, Liu J, Charlton J, Adler P: The furry gene of Drosophila is important for maintaining the integrity of cellular extensions during morphogenesis. Development. 2001, 128: 2793-2802.PubMedGoogle Scholar
- Adler PN, Charlton J, Park WJ: The Drosophila tissue polarity gene inturned functions prior to wing hair morphogenesis in the regulation of hair polarity and number. Genetics. 1994, 137: 829-36.PubMed CentralPubMedGoogle Scholar
- Taylor J, Abramova N, Charlton J, Adler PN: Van Gogh: a new Drosophila tissue polarity gene. Genetics. 1998, 150: 199-210.PubMed CentralPubMedGoogle Scholar
- Lu B, Usui T, Uemura T, Jan L, Jan YN: Flamingo controls the planar polarity of sensory bristles and asymmetric division of sensory organ precursors in Drosophila. Curr Biol. 1999, 9: 1247-50. 10.1016/S0960-9822(99)80505-3.View ArticlePubMedGoogle Scholar
- Weber U, Paricio N, Mlodzik M: Jun mediates Frizzled-induced R3/R4 cell fate distinction and planar polarity determination in the Drosophila eye. Development. 2000, 127: 3619-29.PubMedGoogle Scholar
- Paricio N, Feiguin F, Boutros M, Eaton S, Mlodzik M: The Drosophila STE20-like kinase misshapen is required downstream of the Frizzled receptor in planar polarity signaling. Embo J. 1999, 18: 4669-78. 10.1093/emboj/18.17.4669.PubMed CentralView ArticlePubMedGoogle Scholar
- Boutros M, Paricio N, Strutt DI, Mlodzik M: Dishevelled activates JNK and discriminates between JNK pathways in planar polarity and wingless signaling. Cell. 1998, 94: 109-18.View ArticlePubMedGoogle Scholar
- Geng W, He B, Wang M, Adler PN: The tricornered gene, which encodes the Drosophila NDR kinase is required to maintain the integrity of cellular extensions. Genetics. 2000, 156: 1817-1828.PubMed CentralPubMedGoogle Scholar
- Adler PN, Charlton J, Vinson C: Allelic variation at the frizzled locus of Drosophila. Dev. Genet. 1987, 8: 99-119.View ArticleGoogle Scholar
- Jones KH, Liu J, Adler PN: Molecular analysis of EMS-induced frizzled mutations in Drosophila melanogaster. Genetics. 1996, 142: 205-15.PubMed CentralPubMedGoogle Scholar
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