Conditional and constitutive expression of a Tbx1-GFP fusion protein in mice
© Freyer et al.; licensee BioMed Central Ltd. 2013
Received: 4 March 2013
Accepted: 20 August 2013
Published: 23 August 2013
Velo-cardio-facial syndrome/DiGeorge syndrome (VCFS/DGS) is caused by a 1.5-3 Mb microdeletion of chromosome 22q11.2, frequently referred to as 22q11.2 deletion syndrome (22q11DS). This region includes TBX1, a T-box transcription factor gene that contributes to the etiology of 22q11DS. The requirement for TBX1 in mammalian development is dosage-sensitive, such that loss-of-function (LOF) and gain-of-function (GOF) of TBX1 in both mice and humans results in disease relevant congenital malformations.
To further gain insight into the role of Tbx1 in development, we have targeted the Rosa26 locus to generate a new GOF mouse model in which a Tbx1-GFP fusion protein is expressed conditionally using the Cre/LoxP system. Tbx1-GFP expression is driven by the endogenous Rosa26 promoter resulting in ectopic and persistent expression. Tbx1 is pivotal for proper ear and heart development; ectopic activation of Tbx1-GFP in the otic vesicle by Pax2-Cre and Foxg1-Cre represses neurogenesis and produces morphological defects of the inner ear. Overexpression of a single copy of Tbx1-GFP using Tbx1 Cre/+ was viable, while overexpression of both copies resulted in neonatal lethality with cardiac outflow tract defects. We have partially rescued inner ear and heart anomalies in Tbx1 Cre/- null embryos by expression of Tbx1-GFP.
We have generated a new mouse model to conditionally overexpress a GFP-tagged Tbx1 protein in vivo. This provides a useful tool to investigate in vivo direct downstream targets and protein binding partners of Tbx1.
Velo-cardio-facial syndrome/DiGeorge syndrome (VCFS/DGS), also known as 22q11.2 deletion syndrome (22q11DS), is the most common microdeletion syndrome occurring de novo in approximately 1/4,000 live births . TBX1, encoding a T-box transcription factor, is located within the 1.5 Mb critically deleted region and haploinsufficiency of this gene is responsible for the congenital defects associated with 22q11DS . Tbx1 null mutant mice have malformations of the heart, thymus/parathyroid, craniofacial region, and ear that are similar but more severe, to what is typically found in 22q11DS patients [2–4]. Previously generated BAC316.23 transgenic mice expressing 8–10 copies of human TBX1 also have developmental defects of the same tissues affected in Tbx1 −/− null mice, resembling clinical features of a recently identified 22q11 duplication syndrome [5–9]. Ablation studies using Tbx1 flox conditional null mutants have proven to be indispensible for understanding the tissue and cell-type specific roles of the Tbx1 gene [10, 11]. COET, a transgenic mouse line with conditional overexpression of Tbx1, has previously been reported . However, the COET mouse line does not contain a protein affinity tag on the Tbx1 protein. We have generated a conditional allele targeted at the Rosa26 locus for the purpose of expressing additional copies of Tbx1 fused to a GFP reporter in a tissue-specific manner. We chose a GFP tag in part to detect live GFP as a readout for activation of Tbx1-GFP in vivo and to use it for future biochemical or chromatin immunoprecipitation experiments. Tbx1 mutant rescue experiments demonstrate that Tbx1-GFP fusion protein functions in vivo in a manner similar to that of endogenous Tbx1. The Tbx1-GFP mouse allele provides a new gain-of-function model that is targeted to the constitutively active Rosa26 locus; targeting Tbx1-GFP to the endogenous Tbx1 locus would simultaneously disrupt the normal function of the targeted allele (as is the case for Tbx1 Cre/+ mice, Huynh et al., 2007). As such, our model works to activate 1–2 ectopic copies of Tbx1 while simultaneously tracing cells with GFP, and should therefore be of high value in future experimental studies.
Results and discussion
Since inner ear and cardiac defects are two of the most prominent features in the Tbx1 null mutant, we decided to examine those structures in more detail in the Pax2-Cre;Tbx1-GFP flox/+ mutants and Foxg1-Cre;Tbx1-GFP flox/+ mutants, respectively. Foxg1-Cre and Pax2-Cre are both strongly expressed throughout the OV, from which the inner ear forms. Both loss or gain of Tbx1 function in the OV disrupts inner ear morphogenesis [5, 18, 19]. Paintfilling of the inner ear in Foxg1-Cre;Tbx1-GFP flox/+ and Pax2-Cre;Tbx1-GFP flox/+ mutants at E14.5 shows an enlarged endolymphatic duct (ED) and common crus (CC) that joins the anterior and posterior semicircular canals (SCC) (Figure 2B). The utricle, saccule, and lateral SCC are missing. The cochlea is hypoplastic with varying degree, but enlarged in two instances (Figure 2B). This phenotype is very similar to that of BAC316.23 transgenic mice ; with the exception that BAC316.23 mice still possess the lateral SCC, and in most cases the saccule and utricle are present, albeit malformed (Figure 2B).
Tbx1 is also known to repress neurogenesis of the VIIIth cranial ganglion that innervates the inner ear . Here we show that in Foxg1-Cre;Tbx1-GFP flox/+ mutants, expression of Neurogenic differentiation factor 1 (NeuroD) is nearly abolished in the VIIIth cranial ganglion, and highly attenuated in cranial ganglia V, VII, IX, and X (Figure 2C). Immunohistochemistry with anti-Neurofilament confirmed loss of the VIIIth cranial ganglion and abnormal size and misguided projections from other cranial ganglia in Foxg1-Cre;Tbx1-GFP flox/+ mutants (Figure 2C). In addition, we found that NeuroD expression is completely missing from the olfactory placode of Foxg1-Cre;Tbx1-GFP flox/+ mutants; a region where Foxg1-Cre is active and there is no Tbx1 expression in a wild type context suggesting that gain of function of Tbx1 in the olfactory placode leads to ectopic repression of NeuroD. These findings demonstrate that Tbx1 could act directly or indirectly on neurogenic factors that are common to both otic and olfactory placodes.
In summary, we have generated a new mouse model that conditionally expresses a Tbx1-GFP fusion protein. We show that Tbx1-GFP does not act in a dominant-negative manner and can functionally substitute for endogenous Tbx1 during heart and inner ear development by partial rescue of heart and inner ear morphological defects in a Tbx1 Cre/- null background. We cannot completely rule out deleterious effects of the GFP tag protein on the ability of Tbx1 to act as a transcriptional regulator either via direct binding of DNA or via protein: protein interactions as we have not directly tested this. However, ectopic activation of Tbx1-GFP by Foxg1-Cre and Pax2-Cre causes morphological defects of the inner ear that resemble those occurring in BAC316.23 transgenic mice. Taken together with the partial rescue of heart and inner ear defects on a Tbx1 −/− null background, it seems likely that the Tbx1-GFP fusion protein retains functional characteristics of the endogenous Tbx1 protein. As such, the Tbx1-GFP mouse line provides a genetic tool that is amenable to probing direct downstream targets of Tbx1 and identifying protein-protein interacting partners of Tbx1 in vivo.
A pTbx1-EGFP-N1 plasmid was generated by cloning Tbx1 cDNA from a Tbx1-TOPO plasmid into pEGFP-N1 (Clontech 6085–1). The following oligonucleotide adaptor sequences were used for an in-frame ligation of the C-terminus of Tbx1 to the N-terminus of EGFP, simultaneously changing the Tbx1 stop codon from TAG to TTG and introducing NotI and BamHI restriction enzyme sites: 5’-GGCCGCGCCGCCCGGTGCCTACGACTACTGCCCCAGATTG-3’; 5’GATCCAATCTGGGGCAGTAGTCGTAGGCACCGGGCGGCGC-3’. Adaptors were annealed by combining 10 nmol of each oligonucleotide in 200 μL of water and heating to 90°C followed by cooling at room temperature to 40°C. For 20 μL ligation reactions (Roche 1243292, Roche 10799009001), 5 μL of 1:500 diluted annealed adaptors were used. The sequence encoding the Tbx1-GFP fusion protein was inserted into the multiple cloning site of pBigT . The loxP-tpA-loxP-Tbx1-GFP fragment was excised by restriction enzyme digestion with PacI and AscI then cloned into the pROSA26PA plasmid  to generate the final targeting vector (Figure 1). The final targeting vector was linearized with BbvCI and purified by phenol chloroform extraction, then electroporated into WW6 ESC and selection performed with G418. Positively selected ESC clones were plated in duplicate for DNA isolation using the DNeasy Blood and Tissue Kit (Qiagen 69506). DNA was digested overnight at 37°C with EcoRV and Southern blot performed with a previously described 5’ probe .
The Tbx1-GFP fusion protein was expressed in COS7 cells by transient transfection of the pTbx1-EGFP-N1 plasmid with Polyfect (Qiagen 301105). Protein from whole cell lysate and embryonic tissue was collected in RIPA Lysis Buffer (50 mM Tris HCl pH 7.5, 200 mM NaCl, 1% Triton X-100, 1 mM EDTA, 0.25% deoxycholic acid, protease inhibitor). For tissue samples, the pharyngeal region was isolated from E9.5 embryos and the head, first pharyngeal arch, heart, and caudal part of the embryos (below the fourth pharyngeal pouch) were removed. Tissue was homogenized and lysed in RIPA buffer for 2 hours at 4°C. Samples were run on 7.5 or 10% polyacrylimide gels. Tbx1-GFP fusion protein (≈75 kD) was detected using polyclonal anti-Tbx1 (Zymed, 1:500), anti-GFP (Invitrogen A6455, 1:600), mouse anti-βactin (Abcam ab6276, 1:5,000), donkey anti-Rabbit IgG-HRP (Amersham NA934V, 1:20,000) and sheep anti-Mouse IgG-HRP (Amersham NA931V, 1:5,000) antibodies. Detection was performed using the ECL Western Blotting Detection Kit (Amersham RPN2106) and exposed on Kodak film.
Tbx1-GFP mice were genotyped with the FastStart High Fidelity PCR System (Roche 03553361001) and the following primers: TB3F (5’-CTGCACCACCATCCCTACAA-3’) and GFPR (5’- TGAACTTCAGGGTCAGCTTG-3’) for a 421 bp product from the targeted allele, and RO1F (5’-GCAATACCTTTCTGGGAGTT-3’) and GFP-wtR (5’- CAATGCTCTGTCTAGGGGTT-3’) for a 605 bp product from the wildtype allele. Foxg1-Cre, Pax2-Cre, and Tbx1 Cre/+ mice were kindly provided by Drs. Jean Hebert, Andrew K. Groves, and Antonio Baldini, respectively.. Embryos were dissected according to date of vaginal plug (E0.5). Embryonic stages <E11.5 were confirmed by counting pairs of somites. Animals were maintained in a 12 hr dark/12 hr light cycle in compliance with the Albert Einstein College of Medicine of Yeshiva University Institutional Animal Care and Use Committee (IACUC). Tbx1-GFP mice will become available to the research community upon acceptance of the manuscript.
RNA in situhybridization
Embryos were fixed in 4% paraformaldehyde (PFA) at 4°C overnight then dehydrated in a methanol series. RNA in situ hybridization (ISH) was performed following rehydration to 0.1% PBS/0.1% Tween-20 (PBT) . Anti-sense digoxigenin-labeled RNA probe for NeuroD was generated from a plasmid by standard protocol. The RNA probe template for netrin-1 was generated from amplified E9.5 mouse cDNA using the following primers: Netrin1-Fwd (5’-GGGGAATTAACCCTCACTAAAGGGTGATCCTTGCTCGGATGAGA-3’), Netrin1-Rev (5’-GGGGTAATACGACTCACTATAGGGTTCTTCTCCCGTTGCTGGAA-3’).
Primer sequences introduced T7 RNA polymerase binding sites for generating antisense probes and T3 RNA polymerase binding sites for generating sense probes. For wholemount ISH, we rehydrated embryos to PBT and digested them with Proteinase K. This was followed by washes in Glycine solution and PBT. Embryos were then fixed in 4% PFA/0.2% gluteraldehyde for 15 min on ice followed by PBT washes. Embryos were incubated with RNA probes in hybridization buffer overnight at 70°C then washed in a series of SSC, maleic acid buffer, and PBT washes. Then embryos were incubated overnight at 4°C in antibody buffer with 1:10,000 dilution of anti-Digoxigenin-AP antibody (Roche 11093274910) followed by washes in 0.1% BSA/PBT and AP1 buffer. Staining was performed using BM Purple (Roche 11442074001) followed by fixation in 4% PFA. RNA in situ hybridization to netrin-1 was performed on tissue cryosections according to the David Anderson laboratory protocol. Briefly, tissue sections were fixed in 4% PFA for 20 min at RT and digested with Proteinase K followed by post-fixation in 4% PFA for 15 min. Acetylation was performed in TEA buffer with acetic anhydride followed by washes in PBS and air-drying. Hybridization with RNA probes was done overnight at 68°C in a hyb chamber humidified with 50% formamide/4X SSC. Tissue sections were washed with SSC and PBT then incubated overnight at 4°C in blocking buffer with 1:2,000 dilution of anti-Digoxigenin-AP antibody followed by washes in PBT and AP1 buffer and staining with BM Purple.
Immunofluorescence and whole mount immunohistochemistry
Embryos were fixed overnight in 4% paraformaldehyde, placed in 30% sucrose/PBS, embedded in O.C.T., then cryosectioned at 12 μm thickness. For immunofluorescence, tissue sections were washed in PBS, permeabilized for 5 min in 0.5% Triton X-100, then incubated in blocking solution (5% serum in PBS/0.1% Triton X-100 i.e. PBT) for 1 hour. Primary antibodies (rabbit anti-mouse pan-laminin 1:200, Chemicon AB2034; rabbit anti-Tbx1 1:500, goat anti-GFP 1:500, Abcam ab6673) were diluted in block and incubated on tissue for 1 hr at room temperature. Sections were washed in PBT then incubated with secondary antibody (Alexa Fluor 568 goat anti-rabbit IgG 1:500; Alexa Fluor 568 donkey anti-goat IgG 1:500) for 30 min. DAPI (1:500) was added to secondary antibody. Sections were washed in PBT mounted in Vectashield hard-set mounting medium (Vector Labs H-1400). For whole mount immunohistochemistry, embryos were fixed overnight in 4:1 methanol/DMSO at 4°C. Endogenous peroxidase activity was blocked in 4:1:1 methanol/DMSO/30% H2O2 for 6 hrs at room temperature. Embryos were then rehydrated in a methanol series and blocked in 2% milk/0.5% Triton X-100/PBS (PBSMT) for 1 hour. Primary antibody (anti-neurofilament 1:200, DSHB mAb2H3) was diluted in PBSMT and incubated overnight at 4°C. Embryos were washed five times for an hour each in PBSMT at 4°C and then incubated with [HRP]-sheep anti-mouse IgG (Amersham NA931V, 1:500) diluted in PBSMT overnight at 4°C. Embryos were washed again in PBSMT for 5 hrs at 4°C and then 30 min in 0.2% BSA (Sigma A4503)/0.5% Triton X-100/PBS. Staining was achieved with DAB substrate with chromagen (DAKO K3466) for 15 min at room temperature. Embryos were post-fixed in 4% paraformaldehyde overnight at 4°C, dehydrated in a methanol series, then cleared in 1:2 Benzyl alcohol: Benzyl Benzoate (BABB) for imaging and long-term storage.
Inner ear paintfilling and histology
Embryos were sliced below the forelimbs then fixed in 5% glacial acetic acid, 2% formaldehyde, and 75% ethanol overnight followed by an overnight dehydration in 100% ethanol. Methyl salicylate was then used for clearing of the tissue and long-term storage. Prior to paintfilling, embryos were bisected dorsally and the brain was removed to reveal the inner ear capsule. A micropipette was used to microinject 0.2% correction fluid diluted in methyl salicylate into the inner ear labyrinth. For histological analysis, embryos were fixed in 4% paraformaldehyde overnight at 4°C. They were then dehydrated to 70% ethanol and embedded in paraffin. Tissue sectioning was performed at 10-12 μm thickness. Tissues were cleared in xylene, stained with hematoxylin and eosin (H&E) and then mounted in Permount.
We thank Dr. Frank Constantini for providing the pBigT and pROSA26PA plasmids. We are grateful to the AECOM Transgenic and Gene Targeting Facility. We thank Raquel Castellanos for helpful advice. The work was supported by NIH R01 HL088698 and DC05186 (BEM).
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