Figure 6
Comparison of 32PO 4 uptake by control and progesterone-treated Rana pipiens ovarian follicles. Upper panel: in-vitro [32PO4] uptake by control and progesterone-treated denuded oocytes. Oocytes were incubated in Ringers' solution containing 0.08 mM NaHPO4 and 32PO4 uptake is expressed as μmols/1 cell water. Lower panel: oocytes from the upper panel were homogenized in 7% TCA and protein isolated as described in Methods. 32PO4 uptake into total protein is expressed as μmols/kg wet weight.