Figure 3

TBX3 over-expression promotes cell proliferation. A) Edu cell proliferation assay. The fourth mammary glands from doxycycline induced and un-induced double transgenic mice were harvested at 10.5 dpc and 5μm thick sections were embedded in paraffin and subjected to the Click-iT EdU proliferation Assay (Promega). EdU that had been incorporated into newly synthesized DNA was detected by Alexa Fluor 594 azide (red) and cell nuclei were stained with Hoechst 33342 (blue). All Images were captured at 20× magnification. B) Quantification of Edu positive cells. Fifteen random 20× fields were taken from each group of litter matched doxycycline induced and un-induced double transgenic mice. The proliferating cells were quantified and normalized to the total cell number in each field. The graph is generated from the average ratio (Edu/Hoechst) of fifteen 20× fields in each group. Error bars indicate SD; n = 1. **,P < 0.001.