Figure 6
Determination of specific gene expression profiles using semi-quantitative RT-PCR. Semi-quantitative RT-PCR was used to investigate specific gene expression profiles during early zebrafish development and correlated to the expression profile obtained using RNA-Seq. Left Y axis indicate fold change, while right Y axis indicate RPKM levels. The corresponding method used are given in the graph legend. A) Gene expression profile for plk1, foxA1 and foxA2. B) Gene expression profile for zgc:136359 and slc39a9. C) Gene expression profile for pou5f1, klf4 and foxA3.