Figure 2

Gene clustering based on increasing gene expression profiles across the four developmental stages. Four clusters with an increase in gene expression levels from 1-cell stage to 50% epiboly. A) Cluster 4 contains transcripts with initial low abundance. B) Cluster 5 transcripts (cldn e, slc16a1 and slc25a22 among others) are low in abundance and display a modest increase during development. C) Cluster 9 transcripts (cirbp, ef1a, h3f3a, hspa8, khdrbs1a and bactin2 among others) are relatively abundant at 1-cell stage to increase to RPKM levels above 1000 in 50% epiboly. D) Transcripts in cluster 13 (apoeb, krt18, krt8, krt4 and zgc:85717 among others) are moderately expressed at early stages and increase to high RPKM levels in 50% epiboly.