Figure 6
From: Targets downstream of Cdk8 in Dictyostelium development
Expression of hpd and sdrA in cdk8- 2 cells. Cells were harvested after vegetative growth (0hrs) or development on KK2 agar for 3hrs at 3 x 106 cells/cm2. RNA was extracted from these samples and resolved on a 1% formaldehyde gel, transferred to a nylon membrane and probed with 32P labelled fragments of the hpd and sdrA genes. The blot was reprobed with a 32P labelled fragment of the IG7 gene to control for loading.