Fertilization in C. elegans requires an intact C-terminal RING finger in sperm protein SPE-42

Table 3 C. elegans /C. briggsae spe-42 final exon swap.

Transgene	Progeny	n
ebEx498 xyEx175 xyEx177	92 ± 16 123 ± 15 115 ± 17	11 10 11
xyEx1 xyEx2 xyEx3	2 ± 1 1 ± 0.62 6 ± 2	10 9 9
xyEx27 xyEx30, xyEx34	11 ± 4 8 ± 4 17 ± 4	10 10 10
xyEx36 xyEx37 xyEx41	83 ± 15 110 ± 10 123 ± 15	9 8 10
N2 (wild type) spe-42(tn1231) spe-42(tm2421)	185 ± 5 0 0	10 24 15

Transgenic rescue was quantified for three independently-derived transgenes for each construct. Exon shading scheme is the same as in Fig. 4B. Constructs were fused to the C. elegans promoter and 3' UTR at the start and stop codons. All transgenes are in a spe-42 null genetic background. N2 and spe-42 worms listed at the bottom do not carry transgenes. All counts were conducted until the first day each worm produced no live progeny. Counts are ± SEM rounded to the nearest whole number except for values less than one. The spe-42(tn1231) data are from [13].

ISSN: 1471-213X