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Table 2 Cysteine 678 is not part of the RING finger.

From: Fertilization in C. elegans requires an intact C-terminal RING finger in sperm protein SPE-42

Transgene

Mutation

Progeny

n

xyEx208

C678A

117 ± 22

10

xyEx209

C678A

101 ± 22

9

xyEx212

C678A

113 ± 13

8

xyEx186

C681A

39 ± 6

10

xyEx187

C681A

51 ± 11

7

xyEx188

C681A

63 ± 12

5

xyEx207

C678A; C681A

3 ± 1

10

xyEx213

C678A; C681A

2 ± 1

10

xyEx220

C678A; C681A

1 ± 0.65

10

ebEx498

wild type

92 ± 16

11

xyEx175

wild type

123 ± 15

10

xyEx177

wild type

115 ± 17

11

N2 (wild type)

-

185 ± 5

10

spe-42(tn1231)

-

0

24

spe-42(tm2421)

-

0

15

  1. Counts were conducted as described for table 1.