Fertilization in C. elegans requires an intact C-terminal RING finger in sperm protein SPE-42

Table 1 Transgenic rescue with substitution mutations in RING finger cysteines.

Transgene	Mutation	Progeny	n
xyEx186	C681A	39 ± 6	10
xyEx187	C681A	51 ± 11	7
xyEx188	C681A	63 ± 12	5
xyEx69	C684A	6 ± 2	9
xyEx72	C684A	<1	6
xyEx77	C684A	0	9
xyEx48	C700A	<1	8
xyEx52	C700A	1 ± 0.68	8
xyEx63	C700A	<1	10
xyEx134	C703A	3 ± 1	12
xyEx135	C703A	<1	9
xyEx136	C703A	3 ± 1	10
xyEx73	C708A	0	5
xyEx78	C708A	0	5
xyEx80	C708A	0	5
xyEx74	C711A	<1	9
xyEx81	C711A	3 ± 1	10
xyEx129	C711A	2 ± 0.76	6
xyEx130	C718A	0	9
xyEx131	C718A	<1	9
xyEx132	C718A	0	9
xyEx85	C721A	2 ± 0.60	9
xyEx86	C721A	5 ± 2	10
xyEx87	C721A	11 ± 1	9
ebEx498	wild type	92 ± 16	11
xyEx175	wild type	123 ± 15	10
xyEx177	wild type	115 ± 17	11
N2 (wild type)	-	185 ± 5	10
spe-42(tn1231)	-	0	24
spe-42(tm2421)	-	0	15

Live progeny were counted for three independently-derived transgenes for each amino acid substitution. All transgenes are in a spe-42(tn1231 or tm2421) null genetic background. N2 and spe-42 worms listed at the bottom do not carry transgenes. All counts were conducted until the first day each worm produce no live progeny. Counts shown are ±SEM and were rounded to the nearest whole number with the exception of SEM values less than one. spe-42(tn1231) data are from [13].

ISSN: 1471-213X