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Table 3 Embryo development in relation to the TCF volume injected into the oocytes prior to fertilization.

From: Optimization of somatic cell injection in the perspective of nuclear transfer in goldfish

  

Embryo development (%)

Injected volume

Spawn number

Mid-blastula

24 h stage

Hatching

50 pL

7

76.8 ± 12.4 (a)

67.1 ± 16.6 (a)

50.4 ± 20.3 (a)

10 pL

4

90.6 ± 8.0 (b)

83.2 ± 16.0 (b)

84.8 ± 23.1 (b)

  1. Developments after fertilization are expressed as a percentage of the control oocytes in the same spawn at the same stage (mean ± SE); 25 to 28 oocytes were tested in each sample. TCF trout coelomic fluid. Different letters within rows indicate significant differences (p < 0.05).