Table 2 Embryo development after medium injection into non activated oocytes prior to fertilization.
From: Optimization of somatic cell injection in the perspective of nuclear transfer in goldfish
| Â | Â | Embryo development (%) | ||
|---|---|---|---|---|
Injected medium (50 pL) | Spawn number | Mid-blastula | 24 h stage | Hatching |
Pricked (no injection) | 4 | 97.1 ± 3.6 (a) | 93.5 ± 4.7 (a) | 89.7 ± 12.2 (a) |
PBS | 3 | 27.4 ± 4.2 (b) | 25.7 ± 9.5 (b) | 14.1 ± 7.7 (b) |
Culture medium | 3 | 56.0 ± 21.5 (b) | 42.0 ± 28.0 (b) | 13.0 ± 22.5 (b) |
SOF | 3 | 68.0 ± 18.3 (b) | 52.7 ± 20.2 (b) | 14.3 ± 5.0 (b) |
TCF | 6 | 87.5 ± 5.6 (c) | 76.7 ± 12.8 (b) | 60.4 ± 16.6 (c) |
GCF | 4 | 79.0 ± 15.4 (b, c) | 74.8 ± 14.4 (b) | 13.0 ± 10.4 (b) |
TCF + antibiotics | 6 | 80.6 ± 10.5 (c) | 73.0 ± 19.9 (b) | 57.0 ± 23.2 (c) |