Table 1 Embryo development after oocyte incubation in different media prior to fertilization.
From: Optimization of somatic cell injection in the perspective of nuclear transfer in goldfish
| Â | Â | Embryo development (%) | |
|---|---|---|---|
Incubation medium (30 min 20°C) | Spawn number | 24 h stage | Hatching |
SOF | 6 | 3.9 ± 3.2 (a) | 0 (a) |
GFR | 5 | 3.2 ± 3.0 (a) | 0 (a) |
+ STI 0.1 mg/mL | 10 | 62.0 ± 15.3 (b) | 26.6 ± 8.4 (b) |
+ STI 0,25 mg/mL | 6 | 62.3 ± 18.3 (b) | 26.6 ± 7.0 (b) |
+ STI 0,5 mg/mL | 4 | 44.3 ± 10.0 (b) | 25.7 ± 12.9 (b) |
+ STI 1 mg/mL | 4 | 29.2 ± 18.9 (c) | 19.8 ± 12.4 (b) |
+ STI - BSA 0.5% | 6 | 41.9 ± 28.9 (b, c) | nd |
TCF | 8 | 104.4 ± 6.1 (d) | 105.8 ± 18.5 (d) |