Figure 5
From: Optimization of somatic cell injection in the perspective of nuclear transfer in goldfish
Rupture of fin cell plasma membrane upon injection. A: bright field view showing an intact cell at the tip of the capillary (grey arrow). B: Same view as A under fluorescent light, see the green dot of calcein entrapped into the cell cytoplasm. C: fluorescent view few seconds after cell injection. Four cells were injected in order to get a better representation of the different cases. Two cells were still intact: see the two bright spots of calcein still entrapped into cell cytoplasm, whereas the 2 others had a ruptured membrane: calcein was released and its green fluorescence diffused in the surroundings. Scale bar = 500 μm.