Figure 1
From: Optimization of somatic cell injection in the perspective of nuclear transfer in goldfish
Assessment of spontaneous cortical reaction of oocytes incubated in different media. Goldfish ringer after 5 (A) and 25 min (B) incubation; Synthetic ovarian fluid after 5 (C) and 25 min (D) incubation; goldfish ringer with 0.1 mg/ml STI after 5 (E) and 25 min (F) incubation; trout coelomic fluid after 5 (G) and 25 min (H) incubation. Cortical reaction is visualized by the thin transparent layer appearing around the oocyte. Scale bar = 500 μm.