Figure 3

Pattern of expression of CDT-2::GFP during vulva development. P3.p-P8.p cells express CDT-2 (A and B), but sometimes (30%) P3.p does not (K), perhaps reflecting fusion to hyp7 prior to first cell division (see Figure 1). At four-cell stage, two cells that will not proceed to the third division stop expressing CDT-2 (C, D and K). At Christmas-tree stage CDT-2 is not detected (E and F). Sequential pictures of P6.p undergoing first division showing that during mitosis CDT-2 can be detected in cytoplasm (G and H), and later that CDT-2 is relocalised at the nuclear membrane (I and J). Frequency of expression observed in vulval cells at different stages (K). Black or dark grey circles represent high frequency of expression (100% and 92%). Light grey circles represent a low to medium frequency of expression (18, 30, 36 or 77%). White circles represent undetected or very low frequency of expression (0 or 1%).