Fig. 6

Protein level assessment of HLA-G1/G5 and PGE-2 after 24 h of preconditioning: a A bar graph represents the % Positivity of BM-MSCs responds at 1000 ng/ml, AD and DP-MSCs responds at 2000 ng/ml whereas DP-MSCs shows significant upregulation at 3000 ng/ml and UC-MSCs shows significant upregulation at 3000 ng/ml, b Pictographs represents the histograms for tissue specific hMSCs using BD FACs Diva™ software. Intracellular expression of HLA-G in tissue specific hMSCs; c and d A pictograph and bar graph represent the relative intensity of intracellular HLA-G expression in tissue specific hMSCs. ELISA data for soluble HLA-G5 and PGE-2 in cell culture supernatant at 24 h of Dex preconditioning; e A bar graph represents the BM-MSC responds at 1000 ng/ml, AD-MSCs responds at 2000 ng/ml whereas AD and DP-MSCs showed significant upregulation at 3000 ng/ml and UC-MSCs did not showed significant secretion of sHLA-G, f Bar graph represents that BM-MSCs shows highest expression level at 1000 ng/ml, AD-MSCs at 3000 ng/ml, DP-MSCs at 3000 ng/ml whereas UC-MSCs showed significant upregulation at 1000 ng/ml. Data from three donors in duplicates and shown as mean ± SD; ***p < 0.001, **p < 0.01, *p < 0.05. Scale bar 100 µm