Fig. 4

Etomoxir treatment reduces the levels of acetylation of the male differentiation-specific genomic protein H3K27, leading to downregulation of male differentiation-specific gene expression. a-c Immunofluorescence staining and western blot analysis were performed to examine the expression of H3K27ac and H3k27me3 in the control group and the etomoxir-treated group. (Note: The gels and blots were cropped.) d Changes in the transcription levels of male differentiation-specific genes between the control group and the etomoxir-treated group, as determined by RT-qPCR. Data normalized to 18 s. Greater than nine embryos (samples) from each of the control and experimental groups were tested to obtain the data in d. Perform three independent experiments on the data in d, e. (Bar = 20 μm, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns, no significance; two-tailed unpaired Student’s t-test)